(Circular) (Six-base) MAPSORT of: pFN21AB8822.seq Check: 635 from: 1 to: 5893 pFN21AB8822 5893 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2262 2513 2262 Size: 251 5642 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3236 1937 Size: 1299 4594 Acc65I G'GTAC_C Cuts at: 2488 3214 2488 Size: 726 5167 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 3956 5340 5608 1632 Size: 575 1749 1384 268 1917 Fragments arranged by size: 1917 1749 1384 575 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 4792 5769 1203 Size: 3589 977 1327 Fragments arranged by size: 3589 1327 977 AgeI A'CCGG_T Cuts at: 2267 4724 2267 Size: 2457 3436 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5893 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5893 AlwNI CAG_nnn'CTG Cuts at: 2638 2725 2803 3636 5208 2638 Size: 87 78 833 1572 3323 Fragments arranged by size: 3323 1572 833 87 78 ApaI G_GGCC'C Cuts at: 3171 3171 Size: 5893 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 2942 3202 3428 3580 5689 1493 Size: 1449 260 226 152 2109 1697 Fragments arranged by size: 2109 1697 1449 260 226 152 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 3048 3218 1870 Size: 73 1105 170 4545 Fragments arranged by size: 4545 1105 170 73 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2270 2488 3214 4669 Size: 325 217 627 318 165 218 726 1455 Cuts at: 4669 618 Size: 1842 Fragments arranged by size: 1842 1455 726 627 325 318 218 217 165 BanII G_rGCy'C Cuts at: 1391 2298 2577 2926 3171 3212 1391 Size: 907 279 349 245 41 4072 Fragments arranged by size: 4072 907 349 279 245 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4922 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4084 4118 1903 Size: 34 2147 34 3678 Fragments arranged by size: 3678 2147 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4504 136 Size: 107 122 71 631 605 2832 1525 Fragments arranged by size: 2832 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 2576 2853 3310 2576 Size: 277 457 5159 Fragments arranged by size: 5159 457 277 Bme1580I G_kGCm'C Cuts at: 1790 3171 3954 5110 1790 Size: 1381 783 1156 2573 Fragments arranged by size: 2573 1381 1156 783 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2360 2748 3624 Size: 416 53 83 399 24 943 388 876 Cuts at: 3624 4582 442 Size: 958 1753 Fragments arranged by size: 1753 958 943 876 416 399 388 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2862 4553 1239 Size: 1623 1691 2579 Fragments arranged by size: 2579 1691 1623 Bpu10I CC'TnA_GC Cuts at: 2379 2993 2379 Size: 614 5279 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4018 4883 5181 4018 Size: 865 298 4730 Fragments arranged by size: 4730 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2823 4556 882 Size: 617 1324 1733 2219 Fragments arranged by size: 2219 1733 1324 617 BsaAI yAC'GTr Cuts at: 493 1204 3684 493 Size: 711 2480 2702 Fragments arranged by size: 2702 2480 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 3700 4082 4717 Size: 53 83 186 936 489 1678 382 635 Cuts at: 4717 275 Size: 1451 Fragments arranged by size: 1678 1451 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2267 2926 4326 4724 4998 5145 5556 2267 Size: 659 1400 398 274 147 411 2604 Fragments arranged by size: 2604 1400 659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2346 2376 1178 Size: 30 165 30 943 30 4695 Fragments arranged by size: 4695 943 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2288 2354 1186 Size: 1009 93 66 4725 Fragments arranged by size: 4725 1009 93 66 BseYI C'CCAG_C Cuts at: 2053 2079 2898 5096 5592 2053 Size: 26 819 2198 496 2354 Fragments arranged by size: 2354 2198 819 496 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2197 2240 2485 1163 Size: 573 461 43 245 4571 Fragments arranged by size: 4571 573 461 245 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2435 2926 3111 3954 4039 5110 2124 Size: 311 491 185 843 85 1071 2907 Fragments arranged by size: 2907 1071 843 491 311 185 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2298 2435 2577 2926 3111 3171 Size: 399 334 174 137 142 349 185 60 Cuts at: 3171 3212 3954 4039 5110 1391 Size: 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 349 334 185 174 142 137 85 60 41 BspEI T'CCGG_A Cuts at: 2926 5556 2926 Size: 2630 3263 BspHI T'CATG_A Cuts at: 3782 5512 3782 Size: 1730 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 2262 2513 2724 3248 844 Size: 1337 81 251 211 524 3489 Fragments arranged by size: 3489 1337 524 251 211 81 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2122 2367 4388 4562 65 Size: 1183 874 245 2021 174 1396 Fragments arranged by size: 2021 1396 1183 874 245 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2267 2529 4537 4724 1700 Size: 110 457 262 2008 187 2869 Fragments arranged by size: 2869 2008 457 262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstXI CCAn_nnnn'nTGG Cuts at: 2551 2683 3146 2551 Size: 132 463 5298 Fragments arranged by size: 5298 463 132 BstYI r'GATC_y Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888 Size: 1251 367 385 17 1452 86 12 346 Cuts at: 5888 1972 Size: 1977 Fragments arranged by size: 1977 1452 1251 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2109 513 Size: 552 1044 4297 Fragments arranged by size: 4297 1044 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2329 487 Size: 683 56 1103 4051 Fragments arranged by size: 4051 1103 683 56 BtsI GCAGTG_nn' Cuts at: 2587 3409 4203 4223 2587 Size: 822 794 20 4257 Fragments arranged by size: 4257 822 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3159 3197 3159 Size: 38 5855 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2451 2685 Size: 54 1425 50 275 18 309 312 234 Cuts at: 2685 3340 4229 5858 8 Size: 655 889 1629 43 Fragments arranged by size: 1629 1425 889 655 312 309 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2914 3823 1353 Size: 1561 909 3423 Fragments arranged by size: 3423 1561 909 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3125 4480 4854 5000 387 Size: 1064 138 1536 1355 374 146 1280 Fragments arranged by size: 1536 1355 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2924 2924 Size: 5893 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2862 3956 4553 5340 5608 1239 Size: 393 575 655 1094 597 787 268 1524 Fragments arranged by size: 1524 1094 787 655 597 575 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2281 2281 Size: 5893 EcoO109I rG'GnC_Cy Cuts at: 2750 3167 3168 2750 Size: 417 1 5475 Fragments arranged by size: 5475 417 1 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FseI GG_CCGG'CC Cuts at: 2533 2533 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2346 2378 2820 2852 2921 Size: 32 135 32 774 32 442 32 69 Cuts at: 2921 2953 4534 4566 4608 4640 1373 Size: 32 1581 32 42 32 2626 Fragments arranged by size: 2626 1581 774 442 135 69 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3237 3483 669 Size: 988 281 1299 246 3079 Fragments arranged by size: 3079 1299 988 281 246 HindIII A'AGCT_T Cuts at: 748 2381 748 Size: 1633 4260 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KpnI G_GTAC'C Cuts at: 2492 3218 2492 Size: 726 5167 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2700 5007 5191 1305 Size: 1395 2307 184 2007 Fragments arranged by size: 2307 2007 1395 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 2453 2687 10 Size: 54 1425 343 309 312 234 3216 Fragments arranged by size: 3216 1425 343 312 309 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2549 3851 4210 4369 518 Size: 648 1383 1302 359 159 2042 Fragments arranged by size: 2042 1383 1302 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3309 3986 5134 5379 1606 Size: 1703 677 1148 245 2120 Fragments arranged by size: 2120 1703 1148 677 245 NaeI GCC'GGC Cuts at: 2531 2531 Size: 5893 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5341 NdeI CA'TA_TG Cuts at: 387 3145 387 Size: 2758 3135 NgoMIV G'CCGG_C Cuts at: 2529 2529 Size: 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 3064 3251 4796 1132 Size: 97 1835 187 1545 2229 Fragments arranged by size: 2229 1835 1545 187 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 2725 2803 1108 Size: 1617 78 4198 Fragments arranged by size: 4198 1617 78 PfoI T'CCnGG_A Cuts at: 2718 2718 Size: 5893 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2750 2750 Size: 5893 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PspOMI G'GGCC_C Cuts at: 3167 3167 Size: 5893 PstI C_TGCA'G Cuts at: 830 2285 2510 3245 830 Size: 1455 225 735 3478 Fragments arranged by size: 3478 1455 735 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 SacI G_AGCT'C Cuts at: 2926 2926 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SapI GCTCTTCn'nnn_ Cuts at: 2914 2914 Size: 5893 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SfcI C'TryA_G Cuts at: 826 1045 2281 2493 2506 3241 4376 5057 5248 Size: 219 1236 212 13 735 1135 681 191 Cuts at: 5248 826 Size: 1471 Fragments arranged by size: 1471 1236 1135 735 681 219 212 191 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 2267 1700 Size: 567 5326 SmaI CCC'GGG Cuts at: 1872 3050 3220 1872 Size: 1178 170 4545 Fragments arranged by size: 4545 1178 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 3997 4898 5160 820 Size: 197 926 2054 901 262 1553 Fragments arranged by size: 2054 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5893 StyI C'CwwG_G Cuts at: 513 1065 2136 2406 3332 513 Size: 552 1071 270 926 3074 Fragments arranged by size: 3074 1071 926 552 270 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2810 3920 3937 4090 4275 4720 325 Size: 1255 1230 1110 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1230 1110 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4139 5851 96 Size: 274 80 33 51 1443 2162 1712 138 Fragments arranged by size: 2162 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5853 5885 5853 Size: 32 5861 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2548 2800 2191 Size: 357 252 5284 Fragments arranged by size: 5284 357 252 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3048 3218 1870 Size: 1178 170 4545 Fragments arranged by size: 4545 1178 170 XmnI GAAnn'nnTTC Cuts at: 4022 4022 Size: 5893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI