EMID1 ORF clone pFN21AB8714

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB8714

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Clone info.

Species

human

Clone Name

pFN21AB8714 Sequence (with vector) Restriction Map

Product ID

FHC02957

Flexi Clone PID

FXC02957

ORF Sequence

DNA sequence (1329 bp)
Protein sequence (443 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463024]

Gene

KIBB8714

Symbol

EMID1

Alias : EMI5, EMU1

Description

EMI domain containing 1, transcript variant 1

Original Clone ID

oc00377

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1329 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 443 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33630.1 1.5e-65 100.0 EMID1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011489 33 101 PF07546 EMI domain IPR008160 214 267 PF01391 Collagen triple helix repeat IPR008160 291 344 PF01391 Collagen triple helix repeat IPR008160 315 370 PF01391 Collagen triple helix repeat ProfileScan IPR011489 33 106 PS51041 EMI domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_597712 443 608926 100.0 100.0 NP_001254824 441 608926 99.5 100.0 XP_005261385 445 608926 95.3 100.0 XP_005261386 417 608926 89.0 100.0 XP_011528171 460 608926 96.3 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8714 Download

>KIBB8714  1329 bp
ATGGGCGGCCCGCGGGCTTGGGCGCTGCTCTGCCTCGGGCTCCTGCTCCCGGGAGGCGGC
GCTGCGTGGAGCATCGGGGCAGCTCCGTTCTCCGGACGCAGGAACTGGTGCTCCTATGTG
GTGACCCGCACCATCTCATGCCATGTGCAGAATGGCACCTACCTTCAGCGAGTGCTGCAG
AACTGCCCCTGGCCCATGAGCTGTCCGGGGAGCAGCTACAGAACTGTGGTGAGACCCACA
TACAAGGTGATGTACAAGATAGTGACCGCCCGTGAGTGGAGGTGCTGCCCTGGGCACTCA
GGAGTGAGCTGCGAGGAAGTTGCAGCTTCCTCTGCCTCCTTGGAGCCCATGTGGTCGGGC
AGTACCATGCGGCGGATGGCGCTTCGGCCCACAGCCTTCTCAGGTTGTCTCAACTGCAGC
AAAGTGTCAGAGCTGACAGAGCGGCTGAAGGTGCTGGAGGCCAAGATGACCATGCTGACT
GTCATAGAGCAGCCAGTACCTCCAACACCAGCTACCCCTGAGGACCCTGCCCCGCTCTGG
GGTCCCCCTCCTGCCCAGGGCAGCCCCGGAGATGGAGGCCTCCAGGACCAAGTCGGTGCT
TGGGGGCTTCCCGGGCCCACCGGCCCCAAGGGAGATGCCGGCAGTCGGGGCCCAATGGGG
ATGAGAGGCCCACCAGGTCCACAGGGCCCCCCAGGGAGCCCTGGCCGGGCTGGAGCTGTG
GGCACCCCTGGAGAGAGGGGACCTCCTGGGCCACCAGGGCCTCCTGGCCCCCCTGGGCCC
CCAGCCCCTGTTGGGCCACCCCATGCCCGGATCTCCCAGCATGGAGACCCATTGCTGTCC
AACACCTTCACTGAGACCAACAACCACTGGCCCCAGGGACCCACTGGGCCTCCAGGCCCT
CCAGGGCCCATGGGTCCCCCTGGGCCTCCTGGCCCCACAGGTGTCCCTGGGAGTCCTGGT
CACATAGGACCCCCAGGCCCCACTGGACCCAAAGGAATCTCTGGCCACCCAGGAGAGAAG
GGCGAGAGAGGACTGCGTGGGGAGCCTGGCCCCCAAGGCTCTGCTGGGCAGCGGGGGGAA
CCTGGCCCTAAGGGAGACCCTGGTGAGAAGAGCCACTGGGGGGAGGGGTTGCACCAGCTA
CGCGAGGCTTTGAAGATTTTAGCTGAGAGGGTTTTAATCTTGGAAACAATGATTGGGCTC
TATGAACCAGAGCTGGGGTCTGGGGCGGGCCCTGCCGGCACAGGCACCCCCAGCCTCCTT
CGGGGCAAGAGGGGCGGACATGCAACCAACTACCGGATCGTGGCCCCCAGGAGCCGGGAC
GAGAGAGGC

ORF Protein

Cloned ORF protein sequence for pFN21AB8714 Download

>KIBB8714  443 aa
MGGPRAWALLCLGLLLPGGGAAWSIGAAPFSGRRNWCSYVVTRTISCHVQNGTYLQRVLQ
NCPWPMSCPGSSYRTVVRPTYKVMYKIVTAREWRCCPGHSGVSCEEVAASSASLEPMWSG
STMRRMALRPTAFSGCLNCSKVSELTERLKVLEAKMTMLTVIEQPVPPTPATPEDPAPLW
GPPPAQGSPGDGGLQDQVGAWGLPGPTGPKGDAGSRGPMGMRGPPGPQGPPGSPGRAGAV
GTPGERGPPGPPGPPGPPGPPAPVGPPHARISQHGDPLLSNTFTETNNHWPQGPTGPPGP
PGPMGPPGPPGPTGVPGSPGHIGPPGPTGPKGISGHPGEKGERGLRGEPGPQGSAGQRGE
PGPKGDPGEKSHWGEGLHQLREALKILAERVLILETMIGLYEPELGSGAGPAGTGTPSLL
RGKRGGHATNYRIVAPRSRDERG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8714 Download

>pFN21AB8714  6034 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCGGCCCGCGGGCTTGGGCGCTGCTCTGCCT
CGGGCTCCTGCTCCCGGGAGGCGGCGCTGCGTGGAGCATCGGGGCAGCTCCGTTCTCCGG
ACGCAGGAACTGGTGCTCCTATGTGGTGACCCGCACCATCTCATGCCATGTGCAGAATGG
CACCTACCTTCAGCGAGTGCTGCAGAACTGCCCCTGGCCCATGAGCTGTCCGGGGAGCAG
CTACAGAACTGTGGTGAGACCCACATACAAGGTGATGTACAAGATAGTGACCGCCCGTGA
GTGGAGGTGCTGCCCTGGGCACTCAGGAGTGAGCTGCGAGGAAGTTGCAGCTTCCTCTGC
CTCCTTGGAGCCCATGTGGTCGGGCAGTACCATGCGGCGGATGGCGCTTCGGCCCACAGC
CTTCTCAGGTTGTCTCAACTGCAGCAAAGTGTCAGAGCTGACAGAGCGGCTGAAGGTGCT
GGAGGCCAAGATGACCATGCTGACTGTCATAGAGCAGCCAGTACCTCCAACACCAGCTAC
CCCTGAGGACCCTGCCCCGCTCTGGGGTCCCCCTCCTGCCCAGGGCAGCCCCGGAGATGG
AGGCCTCCAGGACCAAGTCGGTGCTTGGGGGCTTCCCGGGCCCACCGGCCCCAAGGGAGA
TGCCGGCAGTCGGGGCCCAATGGGGATGAGAGGCCCACCAGGTCCACAGGGCCCCCCAGG
GAGCCCTGGCCGGGCTGGAGCTGTGGGCACCCCTGGAGAGAGGGGACCTCCTGGGCCACC
AGGGCCTCCTGGCCCCCCTGGGCCCCCAGCCCCTGTTGGGCCACCCCATGCCCGGATCTC
CCAGCATGGAGACCCATTGCTGTCCAACACCTTCACTGAGACCAACAACCACTGGCCCCA
GGGACCCACTGGGCCTCCAGGCCCTCCAGGGCCCATGGGTCCCCCTGGGCCTCCTGGCCC
CACAGGTGTCCCTGGGAGTCCTGGTCACATAGGACCCCCAGGCCCCACTGGACCCAAAGG
AATCTCTGGCCACCCAGGAGAGAAGGGCGAGAGAGGACTGCGTGGGGAGCCTGGCCCCCA
AGGCTCTGCTGGGCAGCGGGGGGAACCTGGCCCTAAGGGAGACCCTGGTGAGAAGAGCCA
CTGGGGGGAGGGGTTGCACCAGCTACGCGAGGCTTTGAAGATTTTAGCTGAGAGGGTTTT
AATCTTGGAAACAATGATTGGGCTCTATGAACCAGAGCTGGGGTCTGGGGCGGGCCCTGC
CGGCACAGGCACCCCCAGCCTCCTTCGGGGCAAGAGGGGCGGACATGCAACCAACTACCG
GATCGTGGCCCCCAGGAGCCGGGACGAGAGAGGCGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT