Restriction map for
pFN21AB8714
(Circular) (Six-base) MAPSORT of: pFN21AB8714.seq Check: 8547 from: 1 to: 6034
pFN21AB8714 6034 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3377 1937
Size: 1440 4594
Acc65I G'GTAC_C
Cuts at: 3355 3355
Size: 6034
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2471 4097 5481 5749 1632
Size: 521 318 1626 1384 268 1917
Fragments arranged by size:
1917 1626 1384 521 318 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 4933 5910 1203
Size: 3730 977 1327
Fragments arranged by size:
3730 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2387 1184
Size: 1203 4831
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2099 2131 2099
Size: 32 6002
AlwNI CAG_nnn'CTG
Cuts at: 2109 2792 3777 5349 2109
Size: 683 985 1572 2794
Fragments arranged by size:
2794 1572 985 683
ApaI G_GGCC'C
Cuts at: 2622 2657 2693 2784 2913 3236 2622
Size: 35 36 91 129 323 5420
Fragments arranged by size:
5420 323 129 91 36 35
ApaLI G'TGCA_C
Cuts at: 4091 5247 4091
Size: 1156 4878
ApoI r'AATT_y
Cuts at: 1493 3343 3569 3721 5830 1493
Size: 1850 226 152 2109 1697
Fragments arranged by size:
2109 1850 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4588 160
Size: 872 3556 1606
Fragments arranged by size:
3556 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2053 2615 3359 1870
Size: 73 96 14 562 744 4545
Fragments arranged by size:
4545 744 562 96 73 14
BamHI G'GATC_C
Cuts at: 3364 3731 3364
Size: 367 5667
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2726 3248 3355 4810
Size: 325 217 627 372 567 522 107 1455
Cuts at: 4810 618
Size: 1842
Fragments arranged by size:
1842 1455 627 567 522 372 325 217
107
BanII G_rGCy'C
Cuts at: 1391 2046 2352 2622 2657 2693 2705 2784 2913
Size: 655 306 270 35 36 12 79 129
Cuts at: 2913 3204 3236 3353 1391
Size: 291 32 117 4072
Fragments arranged by size:
4072 655 306 291 270 129 117 79
36 35 32 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5063
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2306 2340 4225 4259 1903
Size: 34 369 34 1885 34 3678
Fragments arranged by size:
3678 1885 369 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3926 5136 3926
Size: 1210 4824
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2761 3060 3240
Size: 107 122 71 631 605 1089 299 180
Cuts at: 3240 4645 136
Size: 1405 1525
Fragments arranged by size:
1525 1405 1089 631 605 299 180 122
107 71
BglII A'GATC_T
Cuts at: 6029 6029
Size: 6034
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2301 2622 2657 2693 2729 2784 2913 3236
Size: 511 321 35 36 36 55 129 323
Cuts at: 3236 4095 5251 1790
Size: 859 1156 2573
Fragments arranged by size:
2573 1156 859 511 323 321 129 55
36 36 35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2898 3130 3765
Size: 416 53 83 399 24 1481 232 635
Cuts at: 3765 4723 442
Size: 958 1753
Fragments arranged by size:
1753 1481 958 635 416 399 232 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2763 2763
Size: 6034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 2570 2736 2754 2880 2889 4694 1239
Size: 1241 90 166 18 126 9 1805 2579
Fragments arranged by size:
2579 1805 1241 166 126 90 18 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 5024 5322 4159
Size: 865 298 4871
Fragments arranged by size:
4871 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2230 2823 2852 3093 4697 882
Size: 617 731 593 29 241 1604 2219
Fragments arranged by size:
2219 1604 731 617 593 241 29
BsaAI yAC'GTr
Cuts at: 493 1204 3825 493
Size: 711 2621 2702
Fragments arranged by size:
2702 2621 711
BsaBI GATnn'nnATC
Cuts at: 6028 6028
Size: 6034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3841 4223 4858 275
Size: 53 83 186 936 2308 382 635 1451
Fragments arranged by size:
2308 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2096 3297 4467 4865 5139 5286 5697 2096
Size: 1201 1170 398 274 147 411 2433
Fragments arranged by size:
2433 1201 1170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 2339 2369 2878
Size: 30 165 30 696 30 210 30 509
Cuts at: 2878 2908 2947 2977 1178
Size: 30 39 30 4235
Fragments arranged by size:
4235 696 509 210 165 39 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6034
BseYI C'CCAG_C
Cuts at: 2785 2820 3068 3217 3254 5237 5733 2785
Size: 35 248 149 37 1983 496 3086
Fragments arranged by size:
3086 1983 496 248 149 37 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 1163
Size: 573 435 5026
Fragments arranged by size:
5026 573 435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3484 4245 5273 1540
Size: 275 186 1483 761 1028 2301
Fragments arranged by size:
2301 1483 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2117 4095 4180 5251 2117
Size: 1978 85 1071 2900
Fragments arranged by size:
2900 1978 1071 85
BsmI GAATG_Cn'
Cuts at: 3545 3638 3545
Size: 93 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2117 2301 2352 2622 2657 2693
Size: 399 256 71 184 51 270 35 36
Cuts at: 2693 2705 2729 2784 2913 3204 3236 3353 4095
Size: 12 24 55 129 291 32 117 742
Cuts at: 4095 4180 5251 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 291 270 256 184
129 117 85 71 55 51 36 35
32 24 12
BspEI T'CCGG_A
Cuts at: 2096 5697 2096
Size: 3601 2433
BspHI T'CATG_A
Cuts at: 3923 5653 3923
Size: 1730 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3389 844
Size: 2545 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2446 2539 3921 1394
Size: 429 623 93 1382 3507
Fragments arranged by size:
3507 1382 623 429 93
BsrDI GCAATG_nn'
Cuts at: 65 1248 2834 4529 4703 65
Size: 1183 1586 1695 174 1396
Fragments arranged by size:
1695 1586 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2624 2642 3239 4678 4865 1700
Size: 110 814 18 597 1439 187 2869
Fragments arranged by size:
2869 1439 814 597 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 2256 96
Size: 2160 3874
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6034
BssSI C'ACGA_G
Cuts at: 4094 5106 4094
Size: 1012 5022
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6034
BstBI TT'CG_AA
Cuts at: 4838 4838
Size: 6034
BstEII G'GTnAC_C
Cuts at: 1184 2125 1184
Size: 941 5093
BstYI r'GATC_y
Cuts at: 1972 2814 3364 3731 4116 4133 5585 5671 5683
Size: 842 550 367 385 17 1452 86 12
Cuts at: 5683 6029 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 842 550 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2523 3093 2523
Size: 570 5464
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 2913 513
Size: 552 939 11 898 3634
Fragments arranged by size:
3634 939 898 552 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 3550 4344 4364 3550
Size: 794 20 5220
Fragments arranged by size:
5220 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5999
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2707 3007 3481
Size: 54 1425 50 275 18 877 300 474
Cuts at: 3481 4370 5999 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 877 474 300 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3481 1537
Size: 275 1669 4090
Fragments arranged by size:
4090 1669 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3107 3964 1353
Size: 1754 857 3423
Fragments arranged by size:
3423 1754 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2392 3294 4621 4995 5141 387
Size: 1064 138 803 902 1327 374 146 1280
Fragments arranged by size:
1327 1280 1064 902 803 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2471 2480 2570 2736 2754 2880
Size: 393 521 318 9 90 166 18 126
Cuts at: 2880 2889 4097 4694 5481 5749 1239
Size: 9 1208 597 787 268 1524
Fragments arranged by size:
1524 1208 787 597 521 393 318 268
166 126 90 18 9 9
EcoO109I rG'GnC_Cy
Cuts at: 2527 2546 2653 2689 2690 2744 2762 2781 2882
Size: 19 107 36 1 54 18 19 101
Cuts at: 2882 2900 2909 2918 2972 2981 3233 2527
Size: 18 9 9 54 9 252 5328
Fragments arranged by size:
5328 252 107 101 54 54 36 19
19 18 18 9 9 9 1
EcoRI G'AATT_C
Cuts at: 3343 3721 3343
Size: 378 5656
FspI TGC'GCA
Cuts at: 4540 4540
Size: 6034
HaeII r_GCGC'y
Cuts at: 1678 2030 2067 2387 5181 1678
Size: 352 37 320 2794 2531
Fragments arranged by size:
2794 2531 352 320 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2947 2979 3206 3238 4675
Size: 32 135 32 1375 32 227 32 1437
Cuts at: 4675 4707 4749 4781 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1437 1375 227 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3378 3624 669
Size: 988 281 1440 246 3079
Fragments arranged by size:
3079 1440 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6034
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KpnI G_GTAC'C
Cuts at: 3359 3359
Size: 6034
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2531 2868 5148 5332 1305
Size: 1226 337 2280 184 2007
Fragments arranged by size:
2280 2007 1226 337 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3009 10
Size: 54 1425 343 1177 3035
Fragments arranged by size:
3035 1425 1177 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3992 4351 4510 518
Size: 648 2826 359 159 2042
Fragments arranged by size:
2826 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 3076 3450 4127 5275 5520 1606
Size: 411 1059 374 677 1148 245 2120
Fragments arranged by size:
2120 1148 1059 677 411 374 245
NaeI GCC'GGC
Cuts at: 2644 3241 2644
Size: 597 5437
NcoI C'CATG_G
Cuts at: 513 1065 2004 2913 513
Size: 552 939 909 3634
Fragments arranged by size:
3634 939 909 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NgoMIV G'CCGG_C
Cuts at: 2642 3239 2642
Size: 597 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 3287 3392 4937 1132
Size: 97 2058 105 1545 2229
Fragments arranged by size:
2229 2058 1545 105 97
PasI CC'CwG_GG
Cuts at: 1348 2294 2560 2696 2777 2878 2924 2951 1348
Size: 946 266 136 81 101 46 27 4431
Fragments arranged by size:
4431 946 266 136 101 81 46 27
PciI A'CATG_T
Cuts at: 4933 4933
Size: 6034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6034
PfoI T'CCnGG_A
Cuts at: 2586 2586
Size: 6034
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2099 2131 4103 4135 5616 5648 2099
Size: 32 1972 32 1481 32 2485
Fragments arranged by size:
2485 1972 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2546 2744 2882 2918 2972 2527
Size: 19 198 138 36 54 5589
Fragments arranged by size:
5589 198 138 54 36 19
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6034
PsiI TTA'TAA
Cuts at: 3604 3604
Size: 6034
PspOMI G'GGCC_C
Cuts at: 2618 2653 2689 2780 2909 3232 2618
Size: 35 36 91 129 323 5420
Fragments arranged by size:
5420 323 129 91 36 35
PstI C_TGCA'G
Cuts at: 830 2184 2423 3386 830
Size: 1354 239 963 3478
Fragments arranged by size:
3478 1354 963 239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
SacII CC_GC'GG
Cuts at: 2018 2018
Size: 6034
SalI G'TCGA_C
Cuts at: 1936 3376 1936
Size: 1440 4594
SanDI GG'GwC_CC
Cuts at: 2546 2882 2918 2546
Size: 336 36 5662
Fragments arranged by size:
5662 336 36
SapI GCTCTTCn'nnn_
Cuts at: 3107 3107
Size: 6034
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SexAI A'CCwGG_T
Cuts at: 2677 2677
Size: 6034
SfcI C'TryA_G
Cuts at: 826 1045 2180 2221 2419 3382 4517 5198 5389
Size: 219 1135 41 198 963 1135 681 191
Cuts at: 5389 826
Size: 1471
Fragments arranged by size:
1471 1135 1135 963 681 219 198 191
41
SfiI GGCCn_nnn'nGGCC
Cuts at: 2761 2761
Size: 6034
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 2055 2617 3361 1872
Size: 183 562 744 4545
Fragments arranged by size:
4545 744 562 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4138 5039 5301 820
Size: 197 926 2195 901 262 1553
Fragments arranged by size:
2195 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 3392 1132
Size: 2260 3774
SspI AAT'ATT
Cuts at: 5 52 3958 5
Size: 47 3906 2081
Fragments arranged by size:
3906 2081 47
StuI AGG'CCT
Cuts at: 1517 2583 1517
Size: 1066 4968
StyI C'CwwG_G
Cuts at: 513 1065 2004 2343 2631 2913 3058 3473 513
Size: 552 939 339 288 282 145 415 3074
Fragments arranged by size:
3074 939 552 415 339 288 282 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3027 4061 4078 4231 4416 4861 325
Size: 1255 1447 1034 17 153 185 445 1498
Fragments arranged by size:
1498 1447 1255 1034 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 4280 5992
Size: 274 80 33 51 1443 279 2024 1712
Cuts at: 5992 96
Size: 138
Fragments arranged by size:
2024 1712 1443 279 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2339 2371 2878 2910 5994 6026 2339
Size: 32 507 32 3084 32 2347
Fragments arranged by size:
3084 2347 507 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2594 1316
Size: 1278 4756
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2153 2377 2885 3065 2153
Size: 224 508 180 5122
Fragments arranged by size:
5122 508 224 180
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6034
XmaI C'CCGG_G
Cuts at: 1870 2053 2615 3359 1870
Size: 183 562 744 4545
Fragments arranged by size:
4545 744 562 183
XmnI GAAnn'nnTTC
Cuts at: 4163 4163
Size: 6034
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacI SfoI
SrfI SwaI