DDX43 ORF clone pFN21AB8687

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Halotag ORF Clone pFN21AB8687

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Clone info.

Species

human

Clone Name

pFN21AB8687 Sequence (with vector) Restriction Map

Product ID

FHC02937

Flexi Clone PID

FXC02937

ORF Sequence

DNA sequence (1944 bp)
Protein sequence (648 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463006]

Gene

KIBB8687

Symbol

DDX43

Alias : CT13, HAGE

Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 43

Original Clone ID

oc00350

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1944 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 648 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4977.1 0 99.5 DDX43 CCDS35214.1 1.4e-144 61.3 DDX53 CCDS34240.1 3.6e-64 42.2 DDX46 CCDS75306.1 3.6e-64 42.2 DDX46 CCDS11659.1 4.6e-64 42.1 DDX5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018111 76 128 PF00013 K Homology IPR011545 266 436 PF00270 DNA/RNA helicase IPR001650 511 582 PF00271 Helicase HMMSmart IPR004087 66 133 SM00322 K Homology IPR014001 261 464 SM00487 DEAD-like helicase IPR001650 501 582 SM00490 Helicase ProfileScan IPR004088 67 128 PS50084 K Homology IPR014014 242 270 PS51195 RNA helicase IPR014001 273 448 PS51192 DEAD-like helicase IPR001650 460 621 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_061135 648 606286 99.5 100.0 XP_011534229 529 606286 99.4 81.6 XP_011534230 529 606286 99.4 81.6 XP_011534228 604 606286 92.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8687 Download

>KIBB8687  1944 bp
ATGTCCCACCACGGAGGAGCTCCCAAGGCCTCTACGTGGGTCGTTGCTAGTCGGCGAAGC
TCGACAGTGTCCCGAGCGCCAGAGAGGAGGCCGGCGGAGGAGTTGAATCGAACAGGTCCT
GAGGGATATAGTGTCGGCAGAGGTGGTCGCTGGAGAGGCACCTCTAGGCCCCCGGAGGCC
GTGGCCGCTGGTCACGAGGAACTGCCGCTGTGTTTTGCTTTGAAGAGCCACTTTGTTGGC
GCGGTAATCGGTCGTGGTGGGTCAAAAATAAAGAATATACAAAGTACAACAAACACCACA
ATCCAAATAATACAAGAACAACCAGAATCATTAGTCAAAATTTTTGGCAGCAAGGCAATG
CAAACGAAAGCAAAAGCAGTGATAGACAATTTTGTTAAAAAGCTAGAAGAAAATTACAAT
TCAGAATGCGGAATTGATACTGCATTCCAACCTTCTGTTGGAAAAGATGGAAGCACAGAT
AACAATGTTGTTGCAGGAGATCGGCCATTGATAGATTGGGATCAAATTAGAGAGGAAGGT
TTGAAATGGCAAAAAACAAAGTGGGCAGATTTACCACCAATTAAGAAAAACTTTTATAAA
GAGTCCACTGCCACAAGTGCCATGTCAAAAGTAGAAGCAGATAGTTGGAGGAAAGAAAAT
TTTAATATAACGTGGGATGACTTGAAGGATGGGGAGAAACGACCTATCCCCAATCCTACC
TGCACATTTGATGACGCCTTTCAATGTTATCCTGAGGTTATGGAAAACATTAAAAAGGCA
GGTTTTCAAAAGCCAACACCTATTCAGTCACAGGCATGGCCCATTGTGTTGCAAGGAATA
GATCTTATAGGAGTAGCCCAGACTGGAACAGGAAAGACATTGTGTTATTTAATGCCTGGA
TTTATTCATCTGGTCCTTCAACCCAGCCTTAAAGGTCAAAGGAATAGACCCGGCATGTTA
GTTCTAACTCCCACTCGGGAATTAGCACTTCAAGTAGAAGGAGAATGTTGCAAATATTCA
TATAAAGGGCTTCGGAGTGTTTGTGTATATGGTGGTGGAAATGGAGATGAACAAATAGAA
GAGCTTAAAAAAGGTGTAGATATCATAATTGCAACTCCCGGAAGATTGAATGATCTGCAA
ATGAGTAACTTCGTCAATCTGAAGAATATAACCTACTTGGTTTTAGATGAAGCAGACAAG
ATGTTGGACATGGGATTTGAACCCCAGATAATGAAGATTTTGTTAGATGTGCGCCCAGAT
AGGCAGACAGTTATGACCAGTGCTACATGGCCTCATTCAGTTCATCGCCTCGCACAATCT
TATTTGAAAGAACCAATGATTGTCTATGTTGGTACATTGGATCTAGTTGCTGTAAGTTCA
GTGAAGCAAAATATAATTGTAACCACCGAGGAAGAGAAATGGAGTCACATGCAAACTTTT
CTACAGAGTATGTCATCCACAGACAAAGTCATTGTCTTCGTTTCTCGAAAAGCTGTTGCG
GATCACTTATCAAGTGACCTAATACTTGGAAATATATCAGTAGAGTCTCTGCATGGAGAT
AGAGAACAGAGAGATCGGGAGAAAGCATTAGAGAACTTTAAAACAGGCAAAGTGAGAATA
CTAATTGCAACTGATCTAGCCTCTAGAGGACTTGATGTCCATGACGTTACACATGTCTAT
AATTTTGACTTTCCACGGAATATTGAAGAATACGTACACCGAATAGGGCGCACGGGAAGA
GCAGGGAGGACTGGTGTTTCCATTACAACTTTGACTAGAAATGATTGGAGGGTTGCCTCT
GAATTGATTAATATTCTGGAAAGAGCAAATCAGAGTATTCCAGAGGAGCTTGTATCAATG
GCTGAGAGGTTTGAGGCACATCAACGGAAAAGGGAAATGGAAAGAAAAATGGAAAGACCT
CAAGGAAGGCCCAAGAAGTTTCAT

ORF Protein

Cloned ORF protein sequence for pFN21AB8687 Download

>KIBB8687  648 aa
MSHHGGAPKASTWVVASRRSSTVSRAPERRPAEELNRTGPEGYSVGRGGRWRGTSRPPEA
VAAGHEELPLCFALKSHFVGAVIGRGGSKIKNIQSTTNTTIQIIQEQPESLVKIFGSKAM
QTKAKAVIDNFVKKLEENYNSECGIDTAFQPSVGKDGSTDNNVVAGDRPLIDWDQIREEG
LKWQKTKWADLPPIKKNFYKESTATSAMSKVEADSWRKENFNITWDDLKDGEKRPIPNPT
CTFDDAFQCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPG
FIHLVLQPSLKGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRSVCVYGGGNGDEQIE
ELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDVRPD
RQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTF
LQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRI
LIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRNDWRVAS
ELINILERANQSIPEELVSMAERFEAHQRKREMERKMERPQGRPKKFH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8687 Download

>pFN21AB8687  6649 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCCACCACGGAGGAGCTCCCAAGGCCTCTAC
GTGGGTCGTTGCTAGTCGGCGAAGCTCGACAGTGTCCCGAGCGCCAGAGAGGAGGCCGGC
GGAGGAGTTGAATCGAACAGGTCCTGAGGGATATAGTGTCGGCAGAGGTGGTCGCTGGAG
AGGCACCTCTAGGCCCCCGGAGGCCGTGGCCGCTGGTCACGAGGAACTGCCGCTGTGTTT
TGCTTTGAAGAGCCACTTTGTTGGCGCGGTAATCGGTCGTGGTGGGTCAAAAATAAAGAA
TATACAAAGTACAACAAACACCACAATCCAAATAATACAAGAACAACCAGAATCATTAGT
CAAAATTTTTGGCAGCAAGGCAATGCAAACGAAAGCAAAAGCAGTGATAGACAATTTTGT
TAAAAAGCTAGAAGAAAATTACAATTCAGAATGCGGAATTGATACTGCATTCCAACCTTC
TGTTGGAAAAGATGGAAGCACAGATAACAATGTTGTTGCAGGAGATCGGCCATTGATAGA
TTGGGATCAAATTAGAGAGGAAGGTTTGAAATGGCAAAAAACAAAGTGGGCAGATTTACC
ACCAATTAAGAAAAACTTTTATAAAGAGTCCACTGCCACAAGTGCCATGTCAAAAGTAGA
AGCAGATAGTTGGAGGAAAGAAAATTTTAATATAACGTGGGATGACTTGAAGGATGGGGA
GAAACGACCTATCCCCAATCCTACCTGCACATTTGATGACGCCTTTCAATGTTATCCTGA
GGTTATGGAAAACATTAAAAAGGCAGGTTTTCAAAAGCCAACACCTATTCAGTCACAGGC
ATGGCCCATTGTGTTGCAAGGAATAGATCTTATAGGAGTAGCCCAGACTGGAACAGGAAA
GACATTGTGTTATTTAATGCCTGGATTTATTCATCTGGTCCTTCAACCCAGCCTTAAAGG
TCAAAGGAATAGACCCGGCATGTTAGTTCTAACTCCCACTCGGGAATTAGCACTTCAAGT
AGAAGGAGAATGTTGCAAATATTCATATAAAGGGCTTCGGAGTGTTTGTGTATATGGTGG
TGGAAATGGAGATGAACAAATAGAAGAGCTTAAAAAAGGTGTAGATATCATAATTGCAAC
TCCCGGAAGATTGAATGATCTGCAAATGAGTAACTTCGTCAATCTGAAGAATATAACCTA
CTTGGTTTTAGATGAAGCAGACAAGATGTTGGACATGGGATTTGAACCCCAGATAATGAA
GATTTTGTTAGATGTGCGCCCAGATAGGCAGACAGTTATGACCAGTGCTACATGGCCTCA
TTCAGTTCATCGCCTCGCACAATCTTATTTGAAAGAACCAATGATTGTCTATGTTGGTAC
ATTGGATCTAGTTGCTGTAAGTTCAGTGAAGCAAAATATAATTGTAACCACCGAGGAAGA
GAAATGGAGTCACATGCAAACTTTTCTACAGAGTATGTCATCCACAGACAAAGTCATTGT
CTTCGTTTCTCGAAAAGCTGTTGCGGATCACTTATCAAGTGACCTAATACTTGGAAATAT
ATCAGTAGAGTCTCTGCATGGAGATAGAGAACAGAGAGATCGGGAGAAAGCATTAGAGAA
CTTTAAAACAGGCAAAGTGAGAATACTAATTGCAACTGATCTAGCCTCTAGAGGACTTGA
TGTCCATGACGTTACACATGTCTATAATTTTGACTTTCCACGGAATATTGAAGAATACGT
ACACCGAATAGGGCGCACGGGAAGAGCAGGGAGGACTGGTGTTTCCATTACAACTTTGAC
TAGAAATGATTGGAGGGTTGCCTCTGAATTGATTAATATTCTGGAAAGAGCAAATCAGAG
TATTCCAGAGGAGCTTGTATCAATGGCTGAGAGGTTTGAGGCACATCAACGGAAAAGGGA
AATGGAAAGAAAAATGGAAAGACCTCAAGGAAGGCCCAAGAAGTTTCATGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT