(Circular) (Six-base) MAPSORT of: pFN21AB8687.seq Check: 1009 from: 1 to: 6649 pFN21AB8687 6649 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4459 278 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3992 1937 Size: 2055 4594 Acc65I G'GTAC_C Cuts at: 3970 3970 Size: 6649 AclI AA'CG_TT Cuts at: 1563 4776 5149 1563 Size: 3213 373 3063 Fragments arranged by size: 3213 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3185 4712 6096 6364 1632 Size: 1553 1527 1384 268 1917 Fragments arranged by size: 1917 1553 1527 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6452 AflIII A'CryG_T Cuts at: 1203 3676 5548 6525 1203 Size: 2473 1872 977 1327 Fragments arranged by size: 2473 1872 1327 977 AgeI A'CCGG_T Cuts at: 5480 5480 Size: 6649 AhdI GACnn_n'nnGTC Cuts at: 3659 5378 3659 Size: 1719 4930 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6649 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2378 1184 Size: 1194 5455 AlwNI CAG_nnn'CTG Cuts at: 2123 4392 5964 2123 Size: 2269 1572 2808 Fragments arranged by size: 2808 2269 1572 ApaLI G'TGCA_C Cuts at: 4706 5862 4706 Size: 1156 5493 ApoI r'AATT_y Cuts at: 1493 2343 2662 3958 4184 4336 6445 1493 Size: 850 319 1296 226 152 2109 1697 Fragments arranged by size: 2109 1697 1296 850 319 226 152 AseI AT'TA_AT Cuts at: 160 1032 3813 5203 160 Size: 872 2781 1390 1606 Fragments arranged by size: 2781 1606 1390 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2979 3974 1870 Size: 73 133 903 995 4545 Fragments arranged by size: 4545 995 903 133 73 BamHI G'GATC_C Cuts at: 3979 4346 3979 Size: 367 6282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 3970 5425 618 Size: 325 217 627 375 1808 1455 1842 Fragments arranged by size: 1842 1808 1455 627 375 325 217 BanII G_rGCy'C Cuts at: 1391 2026 3968 1391 Size: 635 1942 4072 Fragments arranged by size: 4072 1942 635 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3472 928 Size: 971 1573 4105 Fragments arranged by size: 4105 1573 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4840 4874 1903 Size: 34 2903 34 3678 Fragments arranged by size: 3678 2903 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4541 5751 4541 Size: 1210 5439 BclI T'GATC_A Cuts at: 1548 1548 Size: 6649 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5260 136 Size: 107 122 71 631 605 3588 1525 Fragments arranged by size: 3588 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2845 6644 2845 Size: 3799 2850 BlpI GC'TnA_GC Cuts at: 4066 4066 Size: 6649 Bme1580I G_kGCm'C Cuts at: 1790 4710 5866 1790 Size: 2920 1156 2573 Fragments arranged by size: 2920 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6649 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4380 5338 442 Size: 416 53 83 399 24 2963 958 1753 Fragments arranged by size: 2963 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 5309 1239 Size: 937 3133 2579 Fragments arranged by size: 3133 2579 937 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3909 4774 5639 5937 3909 Size: 865 865 298 4621 Fragments arranged by size: 4621 865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5312 882 Size: 617 3813 2219 Fragments arranged by size: 3813 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2040 3718 4440 493 Size: 711 836 1678 722 2702 Fragments arranged by size: 2702 1678 836 722 711 BsaBI GATnn'nnATC Cuts at: 2523 6643 2523 Size: 4120 2529 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2739 4456 4838 5473 Size: 53 83 186 936 1206 1717 382 635 Cuts at: 5473 275 Size: 1451 Fragments arranged by size: 1717 1451 1206 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5082 5480 5754 5901 6312 5082 Size: 398 274 147 411 5419 Fragments arranged by size: 5419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3031 3061 3742 3772 1178 Size: 30 165 30 1628 30 681 30 4055 Fragments arranged by size: 4055 1628 681 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2034 2104 2117 3863 1186 Size: 848 70 13 1746 3972 Fragments arranged by size: 3972 1746 848 70 13 BseYI C'CCAG_C Cuts at: 2927 5852 6348 2927 Size: 2925 496 3228 Fragments arranged by size: 3228 2925 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2710 1163 Size: 573 974 5102 Fragments arranged by size: 5102 974 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2257 4099 4860 5888 1540 Size: 275 186 256 1842 761 1028 2301 Fragments arranged by size: 2301 1842 1028 761 275 256 186 BsiHKAI G_wGCw'C Cuts at: 2026 4710 4795 5866 2026 Size: 2684 85 1071 2809 Fragments arranged by size: 2809 2684 1071 85 BsmI GAATG_Cn' Cuts at: 2435 2447 4160 4253 2435 Size: 12 1713 93 4831 Fragments arranged by size: 4831 1713 93 12 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 3968 4710 4795 5866 1391 Size: 399 236 1942 742 85 1071 2174 Fragments arranged by size: 2174 1942 1071 742 399 236 85 BspEI T'CCGG_A Cuts at: 6312 6312 Size: 6649 BspHI T'CATG_A Cuts at: 4538 6268 4538 Size: 1730 4919 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 2774 4004 844 Size: 1888 42 1230 3489 Fragments arranged by size: 3489 1888 1230 42 BsrBI CCG'CTC Cuts at: 1394 1823 4536 1394 Size: 429 2713 3507 Fragments arranged by size: 3507 2713 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2367 5144 5318 65 Size: 1183 1119 2777 174 1396 Fragments arranged by size: 2777 1396 1183 1119 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2095 5293 5480 1700 Size: 110 285 3198 187 2869 Fragments arranged by size: 3198 2869 285 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6649 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6649 BssSI C'ACGA_G Cuts at: 2198 4709 5721 2198 Size: 2511 1012 3126 Fragments arranged by size: 3126 2511 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6649 BstBI TT'CG_AA Cuts at: 5453 5453 Size: 6649 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6649 BstXI CCAn_nnnn'nTGG Cuts at: 2240 2240 Size: 6649 BstYI r'GATC_y Cuts at: 1972 2845 3364 3979 4346 4731 4748 6200 6286 Size: 873 519 615 367 385 17 1452 86 Cuts at: 6286 6298 6644 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 873 615 519 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2124 2757 2124 Size: 633 6016 BtgI C'CryG_G Cuts at: 513 1065 2014 2184 3698 513 Size: 552 949 170 1514 3464 Fragments arranged by size: 3464 1514 949 552 170 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3293 487 Size: 683 56 2067 3843 Fragments arranged by size: 3843 2067 683 56 BtsI GCAGTG_nn' Cuts at: 2388 2610 4165 4959 4979 2388 Size: 222 1555 794 20 4058 Fragments arranged by size: 4058 1555 794 222 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6649 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6614 DraI TTT'AAA Cuts at: 3604 3953 3604 Size: 349 6300 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5656 809 Size: 507 4340 1802 Fragments arranged by size: 4340 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2507 4096 Size: 54 1425 50 275 18 357 320 1589 Cuts at: 4096 4985 6614 8 Size: 889 1629 43 Fragments arranged by size: 1629 1589 1425 889 357 320 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4096 1537 Size: 275 2284 4090 Fragments arranged by size: 4090 2284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 3078 3411 3736 4579 1353 Size: 869 856 333 325 843 3423 Fragments arranged by size: 3423 869 856 843 333 325 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2114 5236 5610 5756 387 Size: 1064 138 525 3122 374 146 1280 Fragments arranged by size: 3122 1280 1064 525 374 146 138 EcoICRI GAG'CTC Cuts at: 2024 2024 Size: 6649 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 3185 4712 5309 6096 6364 1239 Size: 393 544 1009 1527 597 787 268 1524 Fragments arranged by size: 1527 1524 1009 787 597 544 393 268 EcoO109I rG'GnC_Cy Cuts at: 2120 2172 2120 Size: 52 6597 EcoRI G'AATT_C Cuts at: 3958 4336 3958 Size: 378 6271 EcoRV GAT'ATC Cuts at: 3106 3106 Size: 6649 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2251 2611 2251 Size: 360 6289 FspI TGC'GCA Cuts at: 5155 5155 Size: 6649 HaeII r_GCGC'y Cuts at: 1678 2084 5796 1678 Size: 406 3712 2531 Fragments arranged by size: 3712 2531 406 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2153 3497 3529 3629 Size: 32 135 32 549 32 1344 32 100 Cuts at: 3629 3645 3661 3677 3842 3874 5290 5322 5364 Size: 16 16 16 165 32 1416 32 42 Cuts at: 5364 5396 1373 Size: 32 2626 Fragments arranged by size: 2626 1416 1344 549 165 135 100 42 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3993 4239 669 Size: 988 281 2055 246 3079 Fragments arranged by size: 3079 2055 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6649 HpaI GTT'AAC Cuts at: 4239 4239 Size: 6649 KpnI G_GTAC'C Cuts at: 3974 3974 Size: 6649 MfeI C'AATT_G Cuts at: 4248 4248 Size: 6649 MluI A'CGCG_T Cuts at: 6525 6525 Size: 6649 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2443 2476 2630 3189 5763 5947 1305 Size: 1138 33 154 559 2574 184 2007 Fragments arranged by size: 2574 2007 1138 559 184 154 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4827 Fragments arranged by size: 4827 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2818 4607 4966 5125 518 Size: 648 1652 1789 359 159 2042 Fragments arranged by size: 2042 1789 1652 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2192 2212 4065 4742 5890 6135 1606 Size: 586 20 1853 677 1148 245 2120 Fragments arranged by size: 2120 1853 1148 677 586 245 20 NaeI GCC'GGC Cuts at: 2097 2097 Size: 6649 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6097 NdeI CA'TA_TG Cuts at: 387 387 Size: 6649 NgoMIV G'CCGG_C Cuts at: 2095 2095 Size: 6649 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6649 NotI GC'GGCC_GC Cuts at: 4096 4096 Size: 6649 NspI r_CATG'y Cuts at: 1132 1229 2962 3436 3680 4007 5552 1132 Size: 97 1733 474 244 327 1545 2229 Fragments arranged by size: 2229 1733 1545 474 327 244 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6649 PciI A'CATG_T Cuts at: 3676 5548 3676 Size: 1872 4777 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6649 PfoI T'CCnGG_A Cuts at: 3121 3121 Size: 6649 PmeI GTTT'AAAC Cuts at: 3953 3953 Size: 6649 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4718 4750 6231 6263 4718 Size: 32 1481 32 5104 Fragments arranged by size: 5104 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2120 2120 Size: 6649 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6649 PsiI TTA'TAA Cuts at: 2601 4219 2601 Size: 1618 5031 PstI C_TGCA'G Cuts at: 830 4001 830 Size: 3171 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6649 SacI G_AGCT'C Cuts at: 2026 2026 Size: 6649 SalI G'TCGA_C Cuts at: 1936 3991 1936 Size: 2055 4594 SapI GCTCTTCn'nnn_ Cuts at: 2222 3078 3736 2222 Size: 856 658 5135 Fragments arranged by size: 5135 856 658 SbfI CC_TGCA'GG Cuts at: 4001 4001 Size: 6649 ScaI AGT'ACT Cuts at: 4897 6609 4897 Size: 1712 4937 SfcI C'TryA_G Cuts at: 826 1045 3446 3997 5132 5813 6004 826 Size: 219 2401 551 1135 681 191 1471 Fragments arranged by size: 2401 1471 1135 681 551 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6649 SmaI CCC'GGG Cuts at: 1872 3976 1872 Size: 2104 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3924 4753 5654 5916 820 Size: 197 926 1981 829 901 262 1553 Fragments arranged by size: 1981 1553 926 901 829 262 197 SnaBI TAC'GTA Cuts at: 493 3718 4440 493 Size: 3225 722 2702 Fragments arranged by size: 3225 2702 722 SpeI A'CTAG_T Cuts at: 152 152 Size: 6649 SphI G_CATG'C Cuts at: 1132 4007 1132 Size: 2875 3774 SspI AAT'ATT Cuts at: 5 52 3020 3706 3817 4573 5 Size: 47 2968 686 111 756 2081 Fragments arranged by size: 2968 2081 756 686 111 47 StuI AGG'CCT Cuts at: 1517 2033 1517 Size: 516 6133 StyI C'CwwG_G Cuts at: 513 1065 2028 4088 513 Size: 552 963 2060 3074 Fragments arranged by size: 3074 2060 963 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2243 4676 4693 4846 5031 5476 325 Size: 1255 663 2433 17 153 185 445 1498 Fragments arranged by size: 2433 1498 1255 663 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2288 4895 6607 Size: 274 80 33 51 1443 311 2607 1712 Cuts at: 6607 96 Size: 138 Fragments arranged by size: 2607 1712 1443 311 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3031 3063 3742 3774 6609 6641 3031 Size: 32 679 32 2835 32 3039 Fragments arranged by size: 3039 2835 679 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2071 3470 1316 Size: 755 1399 4495 Fragments arranged by size: 4495 1399 755 XbaI T'CTAG_A Cuts at: 3647 3985 3647 Size: 338 6311 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2459 2517 2459 Size: 58 6591 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6649 XmaI C'CCGG_G Cuts at: 1870 3974 1870 Size: 2104 4545 XmnI GAAnn'nnTTC Cuts at: 4778 4778 Size: 6649 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4457 276 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI