UBA3 ORF clone pFN21AB8668

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Halotag ORF Clone pFN21AB8668

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Clone info.

Species

human

Clone Name

pFN21AB8668 Sequence (with vector) Restriction Map

Product ID

FHC02921

Flexi Clone PID

FXC02921

ORF Sequence

DNA sequence (1389 bp)
Protein sequence (463 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463332]

Gene

KIBB8668

Symbol

UBA3

Alias : NAE2, UBE1C, hUBA3

Description

ubiquitin-like modifier activating enzyme 3, transcript variant 1

Original Clone ID

oc00331

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1389 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 463 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2909.1 1.5e-211 99.6 UBA3 CCDS2910.1 1.5e-202 96.5 UBA3 CCDS12439.1 4e-20 41.9 UBA2 CCDS2805.1 8.2e-19 33.9 UBA7 CCDS3516.1 1e-14 34.7 UBA6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000594 69 211 PF00899 UBA/THIF-type NAD/FAD binding fold IPR019572 216 260 PF10585 Ubiquitin-activating enzyme IPR000127 273 334 PF02134 Ubiquitin-activating enzyme repeat IPR014929 374 462 PF08825 E2 binding
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003959 463 603172 99.6 100.0 NP_937838 449 603172 96.5 100.0 XP_011532513 422 603172 90.7 100.0 XP_011532512 436 603172 93.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8668 Download

>KIBB8668  1389 bp
ATGGCGGATGGCGAGGAGCCGGAGAAGAAAAGAAGGAGAATAGAGGAGCTGCTGGCTGAG
AAAATGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGGAACCAT
GTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCACACACCCTGATTTCGAACCGAGCACT
GAATCTCTCCAGTTCTTGTTAGATACATGTAAAGTTCTAGTCATTGGAGCTGGCGGCTTA
GGATGTGAGCTCCTGAAAAATCTGGCCTTGTCTGGTTTTAGACAGATTCATGTTATAGAT
ATGGACACTATAGATGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCCTAAAGATATT
GGAAGACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAACTGCAAT
GTAGTTCCACATTTCTACAAGATTCAAGATTTTAACGACACTTTCTATCGACAATTTCAT
ATTATTGTATGTGGACTGGACTCTATCATCGCCAGAAGATGGATAAATGGCATGCTGATA
TCTCTTCTAAATTATGAAGATGGTGTCTTAGATCCAAGCTCCATTGTCCCTTTGATAGAT
GGGGGGACAGAAGGTTTTAAAGGAAATGCCCGGGTGATTCTGCCTGGAATGACTGCTTGT
ATCGAATGCACGCTGGAACTTTATCCACCACAGGTTAATTTTCCCATGTGCACCATTGCA
TCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGATGTTGCAGTGGCCTAAG
GAGCAGCCTTTTGGAGAAGGGGTTCCATTAGGTGGAGATGATCCTGAACATATACAATGG
ATTTTCCAAAAATCCCTAGAGAGAGCATCACAATATAATATTAGGGGTGTTACGTATAGG
CTCACTCAAGGGGTAGTAAAAAGAATCATTCCTGCAGTAGCTTCCACAAATGCAGTCATT
GCAGCTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAAGTGCATACATTCCCTTGAAT
AATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATACACATTTGAAGCAGAAAGA
AAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTCAAAATATTCAGTTTTCTCCATCAGCT
AAACTACAGGAGGTTTTGGATTATCTAACCAATAGTGCTTCTCTGCAAATGAAATCTCCA
GCCATCACAGCCACCCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCT
ATTGAAGAACGAACAAGGCCAAATCTCTCCAAAACATTGAAAGAATTGGGGCTTGTTGAT
GGACAAGAACTGGCGGTTGCTGATGTCACCACCCCACAGACTGTACTATTCAAACTTCAT
TTTACTTCT

ORF Protein

Cloned ORF protein sequence for pFN21AB8668 Download

>KIBB8668  463 aa
MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPST
ESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDI
GRPKAEVAAEFLNDRVPNCNVVPHFYKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLI
SLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIA
SMPRLPEHCIEYVRMLQWPKEQPFGEGVPLGGDDPEHIQWIFQKSLERASQYNIRGVTYR
LTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAER
KENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTS
IEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8668 Download

>pFN21AB8668  6094 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGATGGCGAGGAGCCGGAGAAGAAAAGAAG
GAGAATAGAGGAGCTGCTGGCTGAGAAAATGGCTGTTGATGGTGGGTGTGGGGACACTGG
AGACTGGGAAGGTCGCTGGAACCATGTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCAC
ACACCCTGATTTCGAACCGAGCACTGAATCTCTCCAGTTCTTGTTAGATACATGTAAAGT
TCTAGTCATTGGAGCTGGCGGCTTAGGATGTGAGCTCCTGAAAAATCTGGCCTTGTCTGG
TTTTAGACAGATTCATGTTATAGATATGGACACTATAGATGTTTCCAATCTAAATAGGCA
GTTTTTATTTAGGCCTAAAGATATTGGAAGACCTAAGGCTGAAGTTGCTGCAGAATTTCT
AAATGACAGAGTTCCTAACTGCAATGTAGTTCCACATTTCTACAAGATTCAAGATTTTAA
CGACACTTTCTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATCGCCAG
AAGATGGATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGTGTCTTAGATCC
AAGCTCCATTGTCCCTTTGATAGATGGGGGGACAGAAGGTTTTAAAGGAAATGCCCGGGT
GATTCTGCCTGGAATGACTGCTTGTATCGAATGCACGCTGGAACTTTATCCACCACAGGT
TAATTTTCCCATGTGCACCATTGCATCTATGCCCAGGCTACCAGAACACTGTATTGAGTA
TGTAAGGATGTTGCAGTGGCCTAAGGAGCAGCCTTTTGGAGAAGGGGTTCCATTAGGTGG
AGATGATCCTGAACATATACAATGGATTTTCCAAAAATCCCTAGAGAGAGCATCACAATA
TAATATTAGGGGTGTTACGTATAGGCTCACTCAAGGGGTAGTAAAAAGAATCATTCCTGC
AGTAGCTTCCACAAATGCAGTCATTGCAGCTGTGTGTGCCACTGAGGTTTTTAAAATAGC
CACAAGTGCATACATTCCCTTGAATAATTACTTGGTGTTTAATGATGTAGATGGGCTGTA
TACATACACATTTGAAGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTCA
AAATATTCAGTTTTCTCCATCAGCTAAACTACAGGAGGTTTTGGATTATCTAACCAATAG
TGCTTCTCTGCAAATGAAATCTCCAGCCATCACAGCCACCCTAGAGGGAAAAAATAGAAC
ACTTTACTTACAGTCGGTAACCTCTATTGAAGAACGAACAAGGCCAAATCTCTCCAAAAC
ATTGAAAGAATTGGGGCTTGTTGATGGACAAGAACTGGCGGTTGCTGATGTCACCACCCC
ACAGACTGTACTATTCAAACTTCATTTTACTTCTGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT