Restriction map for
pFN21AB8668
(Circular) (Six-base) MAPSORT of: pFN21AB8668.seq Check: 7888 from: 1 to: 6094
pFN21AB8668 6094 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 3437 1937
Size: 1122 378 4594
Fragments arranged by size:
4594 1122 378
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2400 4157 5541 5809 1632
Size: 768 1757 1384 268 1917
Fragments arranged by size:
1917 1757 1384 768 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 2210 4993 5970 1203
Size: 1007 2783 977 1327
Fragments arranged by size:
2783 1327 1007 977
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2790 2822 2790
Size: 32 6062
AlwNI CAG_nnn'CTG
Cuts at: 3837 5409 3837
Size: 1572 4522
ApaLI G'TGCA_C
Cuts at: 2713 4151 5307 2713
Size: 1438 1156 3500
Fragments arranged by size:
3500 1438 1156
ApoI r'AATT_y
Cuts at: 1493 2393 3403 3629 3781 5890 1493
Size: 900 1010 226 152 2109 1697
Fragments arranged by size:
2109 1697 1010 900 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2634 3419 1870
Size: 73 195 496 785 4545
Fragments arranged by size:
4545 785 496 195 73
BamHI G'GATC_C
Cuts at: 3424 3791 3424
Size: 367 5727
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3415 4870 618
Size: 325 217 627 1628 1455 1842
Fragments arranged by size:
1842 1628 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2256 3413 1391
Size: 865 1157 4072
Fragments arranged by size:
4072 1157 865
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2374 928
Size: 971 475 4648
Fragments arranged by size:
4648 971 475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 1903
Size: 34 2348 34 3678
Fragments arranged by size:
3678 2348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4705 136
Size: 107 122 71 631 605 3033 1525
Fragments arranged by size:
3033 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 2717 4155 5311 1790
Size: 927 1438 1156 2573
Fragments arranged by size:
2573 1438 1156 927
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2113 3825 4783
Size: 416 53 83 399 24 696 1712 958
Cuts at: 4783 442
Size: 1753
Fragments arranged by size:
1753 1712 958 696 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2118 2177 3186 4754 1239
Size: 879 59 1009 1568 2579
Fragments arranged by size:
2579 1568 1009 879 59
Bpu10I CC'TnA_GC
Cuts at: 2242 2242
Size: 6094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2895 4219 5084 5382 2895
Size: 1324 865 298 3607
Fragments arranged by size:
3607 1324 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4757 882
Size: 617 3258 2219
Fragments arranged by size:
3258 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2898 3885 493
Size: 711 1694 987 2702
Fragments arranged by size:
2702 1694 987 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3901 4283 4918 275
Size: 53 83 186 936 2368 382 635 1451
Fragments arranged by size:
2368 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4527 4925 5199 5346 5757 4527
Size: 398 274 147 411 4864
Fragments arranged by size:
4864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2790 2820 1178
Size: 30 165 30 1387 30 4452
Fragments arranged by size:
4452 1387 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2063 1186
Size: 847 30 5217
Fragments arranged by size:
5217 847 30
BseYI C'CCAG_C
Cuts at: 3097 5297 5793 3097
Size: 2200 496 3398
Fragments arranged by size:
3398 2200 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3544 4305 5333 1540
Size: 275 186 1543 761 1028 2301
Fragments arranged by size:
2301 1543 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2183 2256 2717 4155 4240 5311 2183
Size: 73 461 1438 85 1071 2966
Fragments arranged by size:
2966 1438 1071 461 85 73
BsmI GAATG_Cn'
Cuts at: 2675 3605 3698 2675
Size: 930 93 5071
Fragments arranged by size:
5071 930 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2256 2717 3413 4155 4240 5311
Size: 399 393 73 461 696 742 85 1071
Cuts at: 5311 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 696 461 399 393 85
73
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3449 844
Size: 2605 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3981 1394
Size: 429 2158 3507
Fragments arranged by size:
3507 2158 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2428 2718 2961 4589 4763 65
Size: 1183 1180 290 243 1628 174 1396
Fragments arranged by size:
1628 1396 1183 1180 290 243 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4738 4925 1700
Size: 110 2928 187 2869
Fragments arranged by size:
2928 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2963 794
Size: 2169 3925
BstBI TT'CG_AA
Cuts at: 2172 4898 2172
Size: 2726 3368
BstEII G'GTnAC_C
Cuts at: 1184 3256 1184
Size: 2072 4022
BstYI r'GATC_y
Cuts at: 1972 2575 3424 3791 4176 4193 5645 5731 5743
Size: 603 849 367 385 17 1452 86 12
Cuts at: 5743 6089 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 849 603 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3060 3060
Size: 6094
Bsu36I CC'TnA_GG
Cuts at: 2373 2781 2373
Size: 408 5686
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4603
Fragments arranged by size:
4603 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2497 487
Size: 683 56 1271 4084
Fragments arranged by size:
4084 1271 683 56
BtsI GCAGTG_nn'
Cuts at: 2780 3610 4404 4424 2780
Size: 830 794 20 4450
Fragments arranged by size:
4450 830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 2623 2992 3398 2623
Size: 369 406 5319
Fragments arranged by size:
5319 406 369
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3541 4430 6059
Size: 54 1425 50 275 18 1711 889 1629
Cuts at: 6059 8
Size: 43
Fragments arranged by size:
1711 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2553 4024 1353
Size: 1200 1471 3423
Fragments arranged by size:
3423 1471 1200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4681 5055 5201 387
Size: 1064 138 435 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2254 2254
Size: 6094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2177 2400 3186 4157 4754 5541
Size: 393 486 59 223 786 971 597 787
Cuts at: 5541 5809 1239
Size: 268 1524
Fragments arranged by size:
1524 971 787 786 597 486 393 268
223 59
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
EcoRV GAT'ATC
Cuts at: 2544 2544
Size: 6094
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 5241 1678
Size: 3563 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2238 2270 2567 2599 2855
Size: 32 135 32 666 32 297 32 256
Cuts at: 2855 2887 4735 4767 4809 4841 1373
Size: 32 1848 32 42 32 2626
Fragments arranged by size:
2626 1848 666 297 256 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3438 3684 669
Size: 988 281 1500 246 3079
Fragments arranged by size:
3079 1500 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5208 5392 1305
Size: 3903 184 2007
Fragments arranged by size:
3903 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4272
Fragments arranged by size:
4272 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4052 4411 4570 518
Size: 648 2886 359 159 2042
Fragments arranged by size:
2886 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2969 3510 4187 5335 5580 1606
Size: 1363 541 677 1148 245 2120
Fragments arranged by size:
2120 1363 1148 677 541 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4603
Fragments arranged by size:
4603 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 2214 2539 3452 4997 1132
Size: 97 985 325 913 1545 2229
Fragments arranged by size:
2229 1545 985 913 325 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6094
PciI A'CATG_T
Cuts at: 2210 4993 2210
Size: 2783 3311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2523 2816 1108
Size: 1415 293 4386
Fragments arranged by size:
4386 1415 293
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2790 2822 4163 4195 5676 5708 2790
Size: 32 1341 32 1481 32 3176
Fragments arranged by size:
3176 1481 1341 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PspXI vC'TCGA_Gb
Cuts at: 2138 2138
Size: 6094
PstI C_TGCA'G
Cuts at: 830 2392 2941 3446 830
Size: 1562 549 505 3478
Fragments arranged by size:
3478 1562 549 505
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
PvuII CAG'CTG
Cuts at: 2969 2969
Size: 6094
SacI G_AGCT'C
Cuts at: 2256 2256
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 2313 2388 2937 3149 3442 4577 5258
Size: 219 1268 75 549 212 293 1135 681
Cuts at: 5258 5449 826
Size: 191 1471
Fragments arranged by size:
1471 1268 1135 681 549 293 219 212
191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 2636 3421 1872
Size: 764 785 4545
Fragments arranged by size:
4545 785 764
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2138 2910 4198 5099 5361 820
Size: 197 926 195 772 1288 901 262 1553
Fragments arranged by size:
1553 1288 926 901 772 262 197 195
SnaBI TAC'GTA
Cuts at: 493 2898 3885 493
Size: 2405 987 2702
Fragments arranged by size:
2702 2405 987
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 2539 3452 1132
Size: 1407 913 3774
Fragments arranged by size:
3774 1407 913
SspI AAT'ATT
Cuts at: 5 52 2884 3124 4018 5
Size: 47 2832 240 894 2081
Fragments arranged by size:
2832 2081 894 240 47
StuI AGG'CCT
Cuts at: 1517 2353 1517
Size: 836 5258
StyI C'CwwG_G
Cuts at: 513 1065 2004 3533 513
Size: 552 939 1529 3074
Fragments arranged by size:
3074 1529 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2073 4121 4138 4291 4476 4921 325
Size: 1255 493 2048 17 153 185 445 1498
Fragments arranged by size:
2048 1498 1255 493 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3367 4340 6052
Size: 274 80 33 51 1443 1390 973 1712
Cuts at: 6052 96
Size: 138
Fragments arranged by size:
1712 1443 1390 973 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6054 6086 6054
Size: 32 6062
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XhoI C'TCGA_G
Cuts at: 1943 2138 1943
Size: 195 5899
XmaI C'CCGG_G
Cuts at: 1870 2634 3419 1870
Size: 764 785 4545
Fragments arranged by size:
4545 785 764
XmnI GAAnn'nnTTC
Cuts at: 2805 4223 2805
Size: 1418 4676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI DraIII EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI