MIEF1 ORF clone pFN21AB8301

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB8301

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Clone info.

Species

human

Clone Name

pFN21AB8301 Sequence (with vector) Restriction Map

Product ID

FHC02735

Flexi Clone PID

FXC02735

ORF Sequence

DNA sequence (1434 bp)
Protein sequence (478 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464225]

Gene

KIBB8301

Symbol

MIEF1

Alias : AltMIEF1, HSU79252, MID51, MIEF1-MP, SMCR7L, dJ1104E15.3

Description

mitochondrial elongation factor 1, transcript variant 2

Original Clone ID

oc00077

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1434 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 478 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS77678.1 2.1e-201 99.6 MIEF1 CCDS13995.1 2e-180 99.8 MIEF1 CCDS11193.1 5.9e-54 45.2 MIEF2 CCDS45624.1 6e-54 45.2 MIEF2 CCDS45625.1 4e-11 46.7 MIEF2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001291493 478 615497 99.6 100.0 XP_011528539 463 615497 99.8 90.2 XP_011528540 463 615497 99.8 90.2 NP_061881 463 615497 99.8 90.2 XP_011528538 463 615497 99.8 90.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8301 Download

>KIBB8301  1434 bp
ATGGCAGGCGCTGGTGAGCGCAAAGGCAAGAAGGATGACAATGGCATTGGCACGGCCATT
GACTTTGTGCTCTCCAATGCCCGGCTGGTGCTGGGGGTGGGTGGAGCGGCCATGCTGGGC
ATCGCCACGCTGGCAGTTAAGCGGATGTACGATCGGGCGATCAGTGCCCCTACCAGCCCC
ACCCGCCTGAGCCATTCGGGGAAAAGGAGCTGGGAAGAACCCAACTGGATGGGCTCCCCA
CGACTGCTGAACAGGGACATGAAGACGGGCCTGAGCCGGTCCTTGCAGACCCTTCCCACA
GACTCCTCCACCTTCGACACAGATACATTCTGCCCGCCCCGGCCCAAGCCAGTGGCCAGG
AAGGGCCAGGTAGACTTGAAGAAGTCACGACTCCGCATGTCCCTGCAGGAGAAACTTCTT
ACTTACTACCGGAACCGGGCAGCCATCCCTGCTGGAGAGCAGGCTCGGGCCAAGCAAGCT
GCTGTGGACATATGTGCCGAGCTCCGGAGCTTCCTGCGGGCCAAGTTGCCTGACATGCCG
CTTCGGGACATGTACTTGAGTGGCAGCCTCTACGATGACCTGCAGGTGGTGACAGCTGAC
CACATCCAACTCATTGTGCCCCTTGTGCTGGAGCAGAACCTGTGGTCATGTATTCCTGGT
GAAGACACCATCATGAATGTCCCTGGCTTCTTCCTGGTGCGTCGTGAGAATCCAGAGTAC
TTTCCTCGTGGGAGCAGTTACTGGGACCGCTGTGTAGTAGGGGGCTACCTCTCTCCAAAG
ACAGTCGCAAATACATTTGAGAAGGTAGTGGCTGGCTCCATCAATTGGCCAGCCATAGGG
TCCCTCTTGGACTATGTGATCCGCCCGGCCCCACCCCCAGAAGCCCTCACACTGGAGGTG
CAGTATGAGCGTGACAAACATCTCTTCATTGACTTCCTGCCATCAGTGACCCTCGGTGAC
ACAGTCTTGGTGGCCAAACCACACCGGCTAGCCCAGTATGACAACCTGTGGCGGCTGAGC
CTGCGTCCCGCGGAGACGGCACGCCTGCGGGCTCTGGACCAGGCTGACTCGGGCTGCCGA
TCTCTGTGCCTCAAGATCCTCAAGGCCATATGCAAGTCCACCCCGGCTCTGGGCCACCTC
ACTGCCAGCCAGCTAACCAATGTCATCCTCCACTTGGCCCAGGAGGAGGCTGACTGGTCT
CCGGATATGCTGGCCGACCGTTTCCTGCAGGCCTTGAGGGGACTTATCAGCTACTTAGAG
GCTGGAGTCCTGCCCAGTGCCCTAAACCCCAAGGACAAGGCCTTGAAGGAACGCAGCCTT
AGATATCAGGTGAGGATGATGGAGGTAGACAGTCGACTGAATGTCAGCTGGAAAATCCAG
TCACTAGTTGGGGTTTGGTGGCCATGTTTTCTACCCAGACAGGCCCTGCTTTTC

ORF Protein

Cloned ORF protein sequence for pFN21AB8301 Download

>KIBB8301  478 aa
MAGAGERKGKKDDNGIGTAIDFVLSNARLVLGVGGAAMLGIATLAVKRMYDRAISAPTSP
TRLSHSGKRSWEEPNWMGSPRLLNRDMKTGLSRSLQTLPTDSSTFDTDTFCPPRPKPVAR
KGQVDLKKSRLRMSLQEKLLTYYRNRAAIPAGEQARAKQAAVDICAELRSFLRAKLPDMP
LRDMYLSGSLYDDLQVVTADHIQLIVPLVLEQNLWSCIPGEDTIMNVPGFFLVRRENPEY
FPRGSSYWDRCVVGGYLSPKTVANTFEKVVAGSINWPAIGSLLDYVIRPAPPPEALTLEV
QYERDKHLFIDFLPSVTLGDTVLVAKPHRLAQYDNLWRLSLRPAETARLRALDQADSGCR
SLCLKILKAICKSTPALGHLTASQLTNVILHLAQEEADWSPDMLADRFLQALRGLISYLE
AGVLPSALNPKDKALKERSLRYQVRMMEVDSRLNVSWKIQSLVGVWWPCFLPRQALLF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8301 Download

>pFN21AB8301  6139 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGCGCTGGTGAGCGCAAAGGCAAGAAGGA
TGACAATGGCATTGGCACGGCCATTGACTTTGTGCTCTCCAATGCCCGGCTGGTGCTGGG
GGTGGGTGGAGCGGCCATGCTGGGCATCGCCACGCTGGCAGTTAAGCGGATGTACGATCG
GGCGATCAGTGCCCCTACCAGCCCCACCCGCCTGAGCCATTCGGGGAAAAGGAGCTGGGA
AGAACCCAACTGGATGGGCTCCCCACGACTGCTGAACAGGGACATGAAGACGGGCCTGAG
CCGGTCCTTGCAGACCCTTCCCACAGACTCCTCCACCTTCGACACAGATACATTCTGCCC
GCCCCGGCCCAAGCCAGTGGCCAGGAAGGGCCAGGTAGACTTGAAGAAGTCACGACTCCG
CATGTCCCTGCAGGAGAAACTTCTTACTTACTACCGGAACCGGGCAGCCATCCCTGCTGG
AGAGCAGGCTCGGGCCAAGCAAGCTGCTGTGGACATATGTGCCGAGCTCCGGAGCTTCCT
GCGGGCCAAGTTGCCTGACATGCCGCTTCGGGACATGTACTTGAGTGGCAGCCTCTACGA
TGACCTGCAGGTGGTGACAGCTGACCACATCCAACTCATTGTGCCCCTTGTGCTGGAGCA
GAACCTGTGGTCATGTATTCCTGGTGAAGACACCATCATGAATGTCCCTGGCTTCTTCCT
GGTGCGTCGTGAGAATCCAGAGTACTTTCCTCGTGGGAGCAGTTACTGGGACCGCTGTGT
AGTAGGGGGCTACCTCTCTCCAAAGACAGTCGCAAATACATTTGAGAAGGTAGTGGCTGG
CTCCATCAATTGGCCAGCCATAGGGTCCCTCTTGGACTATGTGATCCGCCCGGCCCCACC
CCCAGAAGCCCTCACACTGGAGGTGCAGTATGAGCGTGACAAACATCTCTTCATTGACTT
CCTGCCATCAGTGACCCTCGGTGACACAGTCTTGGTGGCCAAACCACACCGGCTAGCCCA
GTATGACAACCTGTGGCGGCTGAGCCTGCGTCCCGCGGAGACGGCACGCCTGCGGGCTCT
GGACCAGGCTGACTCGGGCTGCCGATCTCTGTGCCTCAAGATCCTCAAGGCCATATGCAA
GTCCACCCCGGCTCTGGGCCACCTCACTGCCAGCCAGCTAACCAATGTCATCCTCCACTT
GGCCCAGGAGGAGGCTGACTGGTCTCCGGATATGCTGGCCGACCGTTTCCTGCAGGCCTT
GAGGGGACTTATCAGCTACTTAGAGGCTGGAGTCCTGCCCAGTGCCCTAAACCCCAAGGA
CAAGGCCTTGAAGGAACGCAGCCTTAGATATCAGGTGAGGATGATGGAGGTAGACAGTCG
ACTGAATGTCAGCTGGAAAATCCAGTCACTAGTTGGGGTTTGGTGGCCATGTTTTCTACC
CAGACAGGCCCTGCTTTTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT