(Circular) (Six-base) MAPSORT of: pFN21AB8301.seq Check: 9746 from: 1 to: 6139 pFN21AB8301 6139 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2578 2578 Size: 6139 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2376 3351 3358 3482 1937 Size: 439 975 7 124 4594 Fragments arranged by size: 4594 975 439 124 7 Acc65I G'GTAC_C Cuts at: 3460 3460 Size: 6139 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4202 5586 5854 1632 Size: 2570 1384 268 1917 Fragments arranged by size: 2570 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 2553 5038 6015 1203 Size: 1350 2485 977 1327 Fragments arranged by size: 2485 1350 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 3365 4868 3365 Size: 1503 4636 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2423 1184 Size: 1239 4900 AlwNI CAG_nnn'CTG Cuts at: 2015 2644 2745 3430 3882 5454 2015 Size: 629 101 685 452 1572 2700 Fragments arranged by size: 2700 1572 685 629 452 101 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2469 3073 3464 1870 Size: 73 526 604 391 4545 Fragments arranged by size: 4545 604 526 391 73 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3460 4915 618 Size: 325 217 627 1673 1455 1842 Fragments arranged by size: 1842 1673 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2240 2508 3058 3458 1391 Size: 849 268 550 400 4072 Fragments arranged by size: 4072 849 550 400 268 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2273 2673 928 Size: 971 374 400 4394 Fragments arranged by size: 4394 971 400 374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3072 3106 4330 4364 1903 Size: 34 1135 34 1224 34 3678 Fragments arranged by size: 3678 1224 1135 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 4750 136 Size: 107 122 71 631 605 463 2615 1525 Fragments arranged by size: 2615 1525 631 605 463 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3020 3556 3020 Size: 536 5603 Bme1580I G_kGCm'C Cuts at: 1790 2173 2625 3286 4200 5356 1790 Size: 383 452 661 914 1156 2573 Fragments arranged by size: 2573 1156 914 661 452 383 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2755 2992 3273 Size: 416 53 83 399 24 1338 237 281 Cuts at: 3273 3870 4828 442 Size: 597 958 1753 Fragments arranged by size: 1753 1338 958 597 416 399 281 237 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2996 1056 Size: 1940 4199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2478 2654 2918 3288 4799 1239 Size: 1239 176 264 370 1511 2579 Fragments arranged by size: 2579 1511 1239 370 264 176 Bpu10I CC'TnA_GC Cuts at: 2192 2276 2192 Size: 84 6055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2581 3080 3089 3259 4264 5129 5427 2581 Size: 499 9 170 1005 865 298 3293 Fragments arranged by size: 3293 1005 865 499 298 170 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3207 4802 882 Size: 617 1708 1595 2219 Fragments arranged by size: 2219 1708 1595 617 BsaAI yAC'GTr Cuts at: 493 1204 3930 493 Size: 711 2726 2702 Fragments arranged by size: 2726 2702 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3946 4328 4963 275 Size: 53 83 186 936 2413 382 635 1451 Fragments arranged by size: 2413 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2433 2508 3205 4572 4970 5244 5391 5802 2433 Size: 75 697 1367 398 274 147 411 2770 Fragments arranged by size: 2770 1367 697 411 398 274 147 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2891 2921 1178 Size: 30 165 30 1488 30 4396 Fragments arranged by size: 4396 1488 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2299 3203 1186 Size: 1113 904 4122 Fragments arranged by size: 4122 1113 904 BseYI C'CCAG_C Cuts at: 2095 2119 2214 5342 5838 2095 Size: 24 95 3128 496 2396 Fragments arranged by size: 3128 2396 496 95 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2924 1163 Size: 573 1188 4378 Fragments arranged by size: 4378 1188 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2158 3223 3589 4350 5378 1540 Size: 275 186 157 1065 366 761 1028 2301 Fragments arranged by size: 2301 1065 1028 761 366 275 186 157 BsiHKAI G_wGCw'C Cuts at: 2076 2508 4200 4285 5356 2076 Size: 432 1692 85 1071 2859 Fragments arranged by size: 2859 1692 1071 432 85 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 BsmBI CGTCTCn'nnnn_ Cuts at: 3032 3032 Size: 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2173 2240 2508 2625 3058 3286 Size: 399 286 97 67 268 117 433 228 Cuts at: 3286 3458 4200 4285 5356 1391 Size: 172 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 286 268 228 172 117 97 85 67 BspEI T'CCGG_A Cuts at: 2508 3205 5802 2508 Size: 697 2597 2845 Fragments arranged by size: 2845 2597 697 BspHI T'CATG_A Cuts at: 2676 4028 5758 2676 Size: 1352 1730 3057 Fragments arranged by size: 3057 1730 1352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2578 2592 3494 844 Size: 1734 14 902 3489 Fragments arranged by size: 3489 1734 902 14 BsrBI CCG'CTC Cuts at: 1394 1823 2111 4026 1394 Size: 429 288 1915 3507 Fragments arranged by size: 3507 1915 429 288 BsrDI GCAATG_nn' Cuts at: 65 1248 4634 4808 65 Size: 1183 3386 174 1396 Fragments arranged by size: 3386 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2280 2988 4783 4970 1700 Size: 110 470 708 1795 187 2869 Fragments arranged by size: 2869 1795 708 470 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 2730 4199 5211 2730 Size: 1469 1012 3658 Fragments arranged by size: 3658 1469 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6139 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2356 2233 Size: 123 6016 BstYI r'GATC_y Cuts at: 1972 3099 3469 3836 4221 4238 5690 5776 5788 Size: 1127 370 367 385 17 1452 86 12 Cuts at: 5788 6134 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1127 385 370 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3033 513 Size: 552 939 1029 3619 Fragments arranged by size: 3619 1029 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2110 487 Size: 683 56 884 4516 Fragments arranged by size: 4516 884 683 56 BtsI GCAGTG_nn' Cuts at: 3144 3655 4449 4469 3144 Size: 511 794 20 4814 Fragments arranged by size: 4814 794 511 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2112 2358 Size: 54 1425 50 275 18 228 54 246 Cuts at: 2358 2831 2976 3216 3404 3586 4475 6104 8 Size: 473 145 240 188 182 889 1629 43 Fragments arranged by size: 1629 1425 889 473 275 246 240 228 188 182 145 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3586 1537 Size: 275 1774 4090 Fragments arranged by size: 4090 1774 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2933 4069 1353 Size: 1580 1136 3423 Fragments arranged by size: 3423 1580 1136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2855 4726 5100 5246 387 Size: 1064 138 1266 1871 374 146 1280 Fragments arranged by size: 1871 1280 1266 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2506 2506 Size: 6139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2478 2654 2918 3288 4202 4799 5586 Size: 393 846 176 264 370 914 597 787 Cuts at: 5586 5854 1239 Size: 268 1524 Fragments arranged by size: 1524 914 846 787 597 393 370 268 264 176 EcoO109I rG'GnC_Cy Cuts at: 2844 3427 2844 Size: 583 5556 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 EcoRV GAT'ATC Cuts at: 3329 3329 Size: 6139 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2016 5286 1678 Size: 338 3270 2531 Fragments arranged by size: 3270 2531 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2140 2172 3013 3045 3351 Size: 32 135 32 568 32 841 32 306 Cuts at: 3351 3383 4780 4812 4854 4886 1373 Size: 32 1397 32 42 32 2626 Fragments arranged by size: 2626 1397 841 568 306 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3359 3483 3729 669 Size: 988 281 1421 124 246 3079 Fragments arranged by size: 3079 1421 988 281 246 124 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KpnI G_GTAC'C Cuts at: 3464 3464 Size: 6139 MfeI C'AATT_G Cuts at: 2827 3738 2827 Size: 911 5228 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2635 5253 5437 1305 Size: 1330 2618 184 2007 Fragments arranged by size: 2618 2007 1330 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 2833 2978 3406 10 Size: 54 1425 343 528 473 145 428 2743 Fragments arranged by size: 2743 1425 528 473 428 343 145 54 MslI CAynn'nnrTG Cuts at: 518 1166 2675 4097 4456 4615 518 Size: 648 1509 1422 359 159 2042 Fragments arranged by size: 2042 1509 1422 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2600 2754 3035 3372 3555 4232 5380 5625 Size: 994 154 281 337 183 677 1148 245 Cuts at: 5625 1606 Size: 2120 Fragments arranged by size: 2120 1148 994 677 337 281 245 183 154 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4648 Fragments arranged by size: 4648 939 552 NdeI CA'TA_TG Cuts at: 387 2495 3113 387 Size: 2108 618 3413 Fragments arranged by size: 3413 2108 618 NheI G'CTAG_C Cuts at: 1052 2992 1052 Size: 1940 4199 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 2404 2542 2557 3497 5042 1132 Size: 97 1175 138 15 940 1545 2229 Fragments arranged by size: 2229 1545 1175 940 138 97 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 2553 5038 2553 Size: 2485 3654 PflMI CCAn_nnn'nTGG Cuts at: 1108 2829 1108 Size: 1721 4418 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2844 2844 Size: 6139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PstI C_TGCA'G Cuts at: 830 2412 2589 3234 3491 830 Size: 1582 177 645 257 3478 Fragments arranged by size: 3478 1582 645 257 177 PvuI CG_AT'CG Cuts at: 2001 2158 2001 Size: 157 5982 PvuII CAG'CTG Cuts at: 2600 3372 2600 Size: 772 5367 SacI G_AGCT'C Cuts at: 2508 2508 Size: 6139 SacII CC_GC'GG Cuts at: 3036 3036 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3357 3481 1936 Size: 1421 124 4594 Fragments arranged by size: 4594 1421 124 SanDI GG'GwC_CC Cuts at: 2844 2844 Size: 6139 SbfI CC_TGCA'GG Cuts at: 2412 2589 3234 3491 2412 Size: 177 645 257 5060 Fragments arranged by size: 5060 645 257 177 ScaI AGT'ACT Cuts at: 2723 4387 6099 2723 Size: 1664 1712 2763 Fragments arranged by size: 2763 1712 1664 SfcI C'TryA_G Cuts at: 826 1045 2408 2585 3230 3487 4622 5303 5494 Size: 219 1363 177 645 257 1135 681 191 Cuts at: 5494 826 Size: 1471 Fragments arranged by size: 1471 1363 1135 681 645 257 219 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2560 3095 3104 3238 4243 5144 Size: 197 926 617 535 9 134 1005 901 Cuts at: 5144 5406 820 Size: 262 1553 Fragments arranged by size: 1553 1005 926 901 617 535 262 197 134 9 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 3388 152 Size: 3236 2903 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 3236 3305 1517 Size: 1719 69 4351 Fragments arranged by size: 4351 1719 69 StyI C'CwwG_G Cuts at: 513 1065 2004 3294 3578 513 Size: 552 939 1290 284 3074 Fragments arranged by size: 3074 1290 939 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 4166 4183 4336 4521 4966 325 Size: 1255 513 2073 17 153 185 445 1498 Fragments arranged by size: 2073 1498 1255 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 2721 4385 Size: 274 80 33 51 1443 579 165 1664 Cuts at: 4385 6097 96 Size: 1712 138 Fragments arranged by size: 1712 1664 1443 579 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6099 6131 6099 Size: 32 6107 Tth111I GACn'n_nGTC Cuts at: 1316 2966 1316 Size: 1650 4489 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 3130 3389 2086 Size: 1044 259 4836 Fragments arranged by size: 4836 1044 259 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI