BLZF1 ORF clone pFN21AB8115

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Halotag ORF Clone pFN21AB8115

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Clone info.

Species

human

Clone Name

pFN21AB8115 Sequence (with vector) Restriction Map

Product ID

FHC02588

Flexi Clone PID

FXC02588

Original Clone PID

ORK02588

ORF Sequence

DNA sequence (1200 bp)
Protein sequence (400 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463429]

Gene

KIBB8115

Symbol

BLZF1

Alias : GOLGIN-45, JEM-1, JEM-1s, JEM1

Description

basic leucine zipper nuclear factor 1

Original Clone ID

cp00475

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1200 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 400 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1278.1 6.1e-118 100.0 BLZF1 CCDS81397.1 7.6e-43 98.8 BLZF1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013183 181 226 PF08227 Protein of unknown function DUF1721
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011508394 400 608692 100.0 100.0 NP_001307902 400 608692 100.0 100.0 NP_003657 400 608692 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8115 Download

>KIBB8115  1200 bp
ATGACTACTAAAAATTTAGAAACCAAAGTCACCGTTACTTCATCCCCAATCCGAGGAGCA
GGAGATGGAATGGAAACTGAGGAACCACCTAAATCTGTTGAAGTTACCTCCGGAGTCCAA
TCTAGAAAGCATCATAGTCTTCAGAGTCCATGGAAGAAAGCAGTTCCATCAGAGAGCCCA
GGAGTTCTTCAGCTAGGGAAAATGCTCACTGAAAAAGCAATGGAAGTTAAAGCTGTAAGA
ATATTAGTTCCCAAAGCTGCTATAACTCATGATATCCCCAACAAAAATACAAAGGTTAAG
TCTCTGGGACATCATAAAGGAGAATTCCTTGGTCAGTCAGAGGGAGTTATAGAACCTAAT
AAGGAACTCTCAGAGGTAAAGAATGTATTGGAAAAGCTCAAGAATTCTGAAAGAAGGTTA
CTACAGGACAAAGAAGGTCTTTCAAACCAGCTCCGTGTACAGACAGAGGTAAATCGTGAG
TTAAAAAAGTTACTGGTGGCTTCTGTTGGGGATGATCTTCAGTATCACTTTGAACGTCTA
GCCCGTGAGAAAAATCAGCTTATTTTAGAAAATGAAGCCCTAGGTCGAAACACAGCTCAG
CTTTCTGAACAGTTAGAACGTATGTCAATACAGTGTGATGTATGGCGAAGTAAATTCCTT
GCAAGCAGGGTAATGGCAGATGAGTTAACCAACTCAAGAGCAGCTTTACAGCGTCAAAAC
CGTGATGCACACGGGGCTATACAAGATCTCCTAAGTGAACGGGAACAGTTTCGTCAAGAA
ATGATAGCTACCCAGAAATTATTGGAGGAGCTCTTAGTTTCCTTGCAATGGGGAAGAGAG
CAAACTTACTCCCCTAGTGTACAACCCCACAGCACAGCAGAGCTAGCATTAACAAATCAC
AAGTTGGCAAAAGCAGTAAATTCTCATCTTCTGGGAAATGTTGGCATTAACAATCAAAAA
AAGATTCCATCAACAGTTGAATTCTGCAGCACCCCAGCTGAGAAAATGGCTGAAACGGTT
CTAAGAATTTTAGATCCAGTTACCTGCAAAGAGAGTTCACCTGATAATCCATTTTTTGAG
TCTTCACCAACCACCTTACTTGCTACAAAGAAAAATATTGGACGATTTCATCCCTATACT
AGATATGAAAATATAACTTTCAATTGCTGCAATCACTGCCGGGGAGAACTGATTGCCCTT

ORF Protein

Cloned ORF protein sequence for pFN21AB8115 Download

>KIBB8115  400 aa
MTTKNLETKVTVTSSPIRGAGDGMETEEPPKSVEVTSGVQSRKHHSLQSPWKKAVPSESP
GVLQLGKMLTEKAMEVKAVRILVPKAAITHDIPNKNTKVKSLGHHKGEFLGQSEGVIEPN
KELSEVKNVLEKLKNSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERL
AREKNQLILENEALGRNTAQLSEQLERMSIQCDVWRSKFLASRVMADELTNSRAALQRQN
RDAHGAIQDLLSEREQFRQEMIATQKLLEELLVSLQWGREQTYSPSVQPHSTAELALTNH
KLAKAVNSHLLGNVGINNQKKIPSTVEFCSTPAEKMAETVLRILDPVTCKESSPDNPFFE
SSPTTLLATKKNIGRFHPYTRYENITFNCCNHCRGELIAL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8115 Download

>pFN21AB8115  5905 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACTACTAAAAATTTAGAAACCAAAGTCACCGT
TACTTCATCCCCAATCCGAGGAGCAGGAGATGGAATGGAAACTGAGGAACCACCTAAATC
TGTTGAAGTTACCTCCGGAGTCCAATCTAGAAAGCATCATAGTCTTCAGAGTCCATGGAA
GAAAGCAGTTCCATCAGAGAGCCCAGGAGTTCTTCAGCTAGGGAAAATGCTCACTGAAAA
AGCAATGGAAGTTAAAGCTGTAAGAATATTAGTTCCCAAAGCTGCTATAACTCATGATAT
CCCCAACAAAAATACAAAGGTTAAGTCTCTGGGACATCATAAAGGAGAATTCCTTGGTCA
GTCAGAGGGAGTTATAGAACCTAATAAGGAACTCTCAGAGGTAAAGAATGTATTGGAAAA
GCTCAAGAATTCTGAAAGAAGGTTACTACAGGACAAAGAAGGTCTTTCAAACCAGCTCCG
TGTACAGACAGAGGTAAATCGTGAGTTAAAAAAGTTACTGGTGGCTTCTGTTGGGGATGA
TCTTCAGTATCACTTTGAACGTCTAGCCCGTGAGAAAAATCAGCTTATTTTAGAAAATGA
AGCCCTAGGTCGAAACACAGCTCAGCTTTCTGAACAGTTAGAACGTATGTCAATACAGTG
TGATGTATGGCGAAGTAAATTCCTTGCAAGCAGGGTAATGGCAGATGAGTTAACCAACTC
AAGAGCAGCTTTACAGCGTCAAAACCGTGATGCACACGGGGCTATACAAGATCTCCTAAG
TGAACGGGAACAGTTTCGTCAAGAAATGATAGCTACCCAGAAATTATTGGAGGAGCTCTT
AGTTTCCTTGCAATGGGGAAGAGAGCAAACTTACTCCCCTAGTGTACAACCCCACAGCAC
AGCAGAGCTAGCATTAACAAATCACAAGTTGGCAAAAGCAGTAAATTCTCATCTTCTGGG
AAATGTTGGCATTAACAATCAAAAAAAGATTCCATCAACAGTTGAATTCTGCAGCACCCC
AGCTGAGAAAATGGCTGAAACGGTTCTAAGAATTTTAGATCCAGTTACCTGCAAAGAGAG
TTCACCTGATAATCCATTTTTTGAGTCTTCACCAACCACCTTACTTGCTACAAAGAAAAA
TATTGGACGATTTCATCCCTATACTAGATATGAAAATATAACTTTCAATTGCTGCAATCA
CTGCCGGGGAGAACTGATTGCCCTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT