(Circular) (Six-base) MAPSORT of: pFN21AB8115.seq Check: 2027 from: 1 to: 5905 pFN21AB8115 5905 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 2177 2507 3968 5352 5620 1632 Size: 497 48 330 1461 1384 268 1917 Fragments arranged by size: 1917 1461 1384 497 330 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 4804 5781 1203 Size: 3601 977 1327 Fragments arranged by size: 3601 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 3648 5220 3648 Size: 1572 4333 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2017 2327 2407 2657 2923 2984 3030 3214 Size: 524 310 80 250 266 61 46 184 Cuts at: 3214 3440 3592 5701 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 524 310 266 250 226 184 152 80 61 46 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 AvrII C'CTAG_G Cuts at: 2584 2584 Size: 5905 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3226 4681 618 Size: 325 217 627 1439 1455 1842 Fragments arranged by size: 1842 1455 1439 627 325 217 BanII G_rGCy'C Cuts at: 1391 2183 2817 3224 1391 Size: 792 634 407 4072 Fragments arranged by size: 4072 792 634 407 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2135 3078 928 Size: 971 236 943 3755 Fragments arranged by size: 3755 971 943 236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2749 5900 2749 Size: 3151 2754 BlpI GC'TnA_GC Cuts at: 2601 3322 2601 Size: 721 5184 Bme1580I G_kGCm'C Cuts at: 1790 3966 5122 1790 Size: 2176 1156 2573 Fragments arranged by size: 2573 2176 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3636 4594 442 Size: 416 53 83 399 24 2219 958 1753 Fragments arranged by size: 2219 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2891 1056 Size: 1835 4070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4565 1239 Size: 3326 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 2683 4030 4895 5193 2387 Size: 296 1347 865 298 3099 Fragments arranged by size: 3099 1347 865 298 296 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3712 4094 4729 275 Size: 53 83 186 936 2179 382 635 1451 Fragments arranged by size: 2179 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2114 4338 4736 5010 5157 5568 2114 Size: 2224 398 274 147 411 2451 Fragments arranged by size: 2451 2224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2736 2766 1178 Size: 30 165 30 1333 30 4317 Fragments arranged by size: 4317 1333 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2073 2825 1186 Size: 887 752 4266 Fragments arranged by size: 4266 887 752 BseYI C'CCAG_C Cuts at: 2998 5108 5604 2998 Size: 2110 496 3299 Fragments arranged by size: 3299 2110 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5332 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2817 3966 4051 5122 2817 Size: 1149 85 1071 3600 Fragments arranged by size: 3600 1149 1071 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2817 3224 3966 4051 5122 1391 Size: 399 393 634 407 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 634 407 399 393 85 BspEI T'CCGG_A Cuts at: 2114 5568 2114 Size: 3454 2451 BspHI T'CATG_A Cuts at: 2272 3794 5524 2272 Size: 1522 1730 2653 Fragments arranged by size: 2653 1730 1522 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3056 3260 844 Size: 2212 204 3489 Fragments arranged by size: 3489 2212 204 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2229 2837 4400 4574 65 Size: 1183 981 608 1563 174 1396 Fragments arranged by size: 1563 1396 1183 981 608 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4549 4736 1700 Size: 110 2739 187 2869 Fragments arranged by size: 2869 2739 187 110 BsrGI T'GTAC_A Cuts at: 96 2461 2863 96 Size: 2365 402 3138 Fragments arranged by size: 3138 2365 402 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 3180 794 Size: 2386 3519 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2749 3037 3235 3602 3987 4004 5456 5542 Size: 777 288 198 367 385 17 1452 86 Cuts at: 5542 5554 5900 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 777 385 367 346 288 198 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2153 513 Size: 552 1088 4265 Fragments arranged by size: 4265 1088 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 3178 3421 4215 4235 3178 Size: 243 794 20 4848 Fragments arranged by size: 4848 794 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3085 3120 529 Size: 35 2521 35 3314 Fragments arranged by size: 3314 2521 35 35 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3352 4241 5870 Size: 54 1425 50 275 18 1522 889 1629 Cuts at: 5870 8 Size: 43 Fragments arranged by size: 1629 1522 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2833 3835 1353 Size: 1480 1002 3423 Fragments arranged by size: 3423 1480 1002 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2815 2815 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2177 2507 3968 4565 5352 5620 Size: 393 497 48 330 1461 597 787 268 Cuts at: 5620 1239 Size: 1524 Fragments arranged by size: 1524 1461 787 597 497 393 330 268 48 EcoRI G'AATT_C Cuts at: 2327 2407 2984 3214 3592 2327 Size: 80 577 230 378 4640 Fragments arranged by size: 4640 577 378 230 80 EcoRV GAT'ATC Cuts at: 2278 2278 Size: 5905 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2459 2724 2459 Size: 265 5640 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 5052 1678 Size: 3374 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373 Size: 32 135 32 2974 32 42 32 2626 Fragments arranged by size: 2974 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2691 3249 3495 669 Size: 988 281 753 558 246 3079 Fragments arranged by size: 3079 988 753 558 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 2691 3495 2691 Size: 804 5101 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3166 3504 3166 Size: 338 5567 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5019 5203 1305 Size: 3714 184 2007 Fragments arranged by size: 3714 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4083 Fragments arranged by size: 4083 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3863 4222 4381 518 Size: 648 2697 359 159 2042 Fragments arranged by size: 2697 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3002 3321 3998 5146 5391 1606 Size: 1396 319 677 1148 245 2120 Fragments arranged by size: 2120 1396 1148 677 319 245 NcoI C'CATG_G Cuts at: 513 1065 2153 513 Size: 552 1088 4265 Fragments arranged by size: 4265 1088 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 2887 1052 Size: 1835 4070 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PstI C_TGCA'G Cuts at: 830 2993 3257 830 Size: 2163 264 3478 Fragments arranged by size: 3478 2163 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 3002 3002 Size: 5905 SacI G_AGCT'C Cuts at: 2817 2817 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2426 2989 3253 4388 5069 5260 826 Size: 219 1381 563 264 1135 681 191 1471 Fragments arranged by size: 1471 1381 1135 681 563 264 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 2698 4009 4910 5172 820 Size: 197 926 459 296 1311 901 262 1553 Fragments arranged by size: 1553 1311 926 901 459 296 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 2247 3121 3829 5 Size: 47 2195 874 708 2081 Fragments arranged by size: 2195 2081 874 708 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2153 2332 2584 3344 513 Size: 552 1088 179 252 760 3074 Fragments arranged by size: 3074 1088 760 552 252 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3932 3949 4102 4287 4732 325 Size: 1255 2352 17 153 185 445 1498 Fragments arranged by size: 2352 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 2863 4151 Size: 274 80 33 51 1443 484 402 1288 Cuts at: 4151 5863 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1288 484 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 2126 3241 2126 Size: 1115 4790 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 2772 3021 4034 2772 Size: 249 1013 4643 Fragments arranged by size: 4643 1013 249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI