ISL1 ORF clone pFN21AB7691

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Halotag ORF Clone pFN21AB7691

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Clone info.

Species

human

Clone Name

pFN21AB7691 Sequence (with vector) Restriction Map

Product ID

FHC11363

Flexi Clone PID

FXC11363

ORF Sequence

DNA sequence (1047 bp)
Protein sequence (349 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528956]

Gene

KIBB7691

Symbol

ISL1

Alias : ISLET1, Isl-1

Description

ISL LIM homeobox 1

Original Clone ID

cs00191

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1047 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 349 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43314.1 1.9e-83 100.0 ISL1 CCDS10290.1 6.4e-63 75.6 ISL2 CCDS1247.1 2e-16 35.0 LMX1A CCDS55343.1 3.6e-16 32.5 LMX1B CCDS6866.2 2.4e-15 32.3 LMX1B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001781 17 74 PF00412 Zinc finger IPR001781 79 133 PF00412 Zinc finger IPR001356 183 238 PF00046 Homeobox HMMSmart IPR001781 16 70 SM00132 Zinc finger IPR001781 78 132 SM00132 Zinc finger IPR001356 181 243 SM00389 Homeobox ProfileScan IPR001781 15 77 PS50023 Zinc finger IPR001781 78 139 PS50023 Zinc finger IPR001356 179 239 PS50071 Homeobox
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002193 349 600366 100.0 100.0 NP_665804 359 609481 75.6 99.7 XP_016877994 534 609481 75.5 91.1 NP_001167540 382 600298 35.0 84.8 NP_796372 382 600298 35.0 84.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7691 Download

>KIBB7691  1047 bp
ATGGGAGACATGGGAGATCCACCAAAAAAAAAACGTCTGATTTCCCTATGTGTTGGTTGC
GGCAATCAGATTCACGATCAGTATATTCTGAGGGTTTCTCCGGATTTGGAATGGCATGCG
GCATGTTTGAAATGTGCGGAGTGTAATCAGTATTTGGACGAGAGCTGTACATGCTTTGTT
AGGGATGGGAAAACCTACTGTAAAAGAGATTATATCAGGTTGTACGGGATCAAATGCGCC
AAGTGCAGCATCGGCTTCAGCAAGAACGACTTCGTGATGCGTGCCCGCTCCAAGGTGTAT
CACATCGAGTGTTTCCGCTGTGTGGCCTGCAGCCGCCAGCTCATCCCTGGGGACGAATTT
GCGCTTCGGGAGGACGGTCTCTTCTGCCGAGCAGACCACGATGTGGTGGAGAGGGCCAGT
CTAGGCGCTGGCGACCCGCTCAGTCCCCTGCATCCAGCGCGGCCACTGCAAATGGCAGCG
GAGCCCATCTCCGCCAGGCAGCCAGCCCTGCGGCCCCACGTCCACAAGCAGCCGGAGAAG
ACCACCCGCGTGCGGACTGTGCTGAACGAGAAGCAGCTGCACACCTTGCGGACCTGCTAC
GCCGCAAACCCGCGGCCAGATGCGCTCATGAAGGAGCAACTGGTAGAGATGACGGGCCTC
AGTCCCCGTGTGATCCGGGTCTGGTTTCAAAACAAGCGGTGCAAGGACAAGAAGCGAAGC
ATCATGATGAAGCAACTCCAGCAGCAGCAGCCCAATGACAAAACTAATATCCAGGGGATG
ACAGGAACTCCCATGGTGGCTGCCAGTCCAGAGAGACACGACGGTGGCTTACAGGCTAAC
CCAGTGGAAGTACAAAGTTACCAGCCACCTTGGAAAGTACTGAGCGACTTCGCCTTGCAG
AGTGACATAGATCAGCCTGCTTTTCAGCAACTGGTCAATTTTTCAGAAGGAGGACCGGGC
TCTAATTCCACTGGCAGTGAAGTAGCATCAATGTCCTCTCAACTTCCAGATACACCTAAC
AGCATGGTAGCCAGTCCTATTGAGGCA

ORF Protein

Cloned ORF protein sequence for pFN21AB7691 Download

>KIBB7691  349 aa
MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFV
RDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEF
ALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEK
TTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRS
IMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQ
SDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7691 Download

>pFN21AB7691  5752 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGAGACATGGGAGATCCACCAAAAAAAAAACG
TCTGATTTCCCTATGTGTTGGTTGCGGCAATCAGATTCACGATCAGTATATTCTGAGGGT
TTCTCCGGATTTGGAATGGCATGCGGCATGTTTGAAATGTGCGGAGTGTAATCAGTATTT
GGACGAGAGCTGTACATGCTTTGTTAGGGATGGGAAAACCTACTGTAAAAGAGATTATAT
CAGGTTGTACGGGATCAAATGCGCCAAGTGCAGCATCGGCTTCAGCAAGAACGACTTCGT
GATGCGTGCCCGCTCCAAGGTGTATCACATCGAGTGTTTCCGCTGTGTGGCCTGCAGCCG
CCAGCTCATCCCTGGGGACGAATTTGCGCTTCGGGAGGACGGTCTCTTCTGCCGAGCAGA
CCACGATGTGGTGGAGAGGGCCAGTCTAGGCGCTGGCGACCCGCTCAGTCCCCTGCATCC
AGCGCGGCCACTGCAAATGGCAGCGGAGCCCATCTCCGCCAGGCAGCCAGCCCTGCGGCC
CCACGTCCACAAGCAGCCGGAGAAGACCACCCGCGTGCGGACTGTGCTGAACGAGAAGCA
GCTGCACACCTTGCGGACCTGCTACGCCGCAAACCCGCGGCCAGATGCGCTCATGAAGGA
GCAACTGGTAGAGATGACGGGCCTCAGTCCCCGTGTGATCCGGGTCTGGTTTCAAAACAA
GCGGTGCAAGGACAAGAAGCGAAGCATCATGATGAAGCAACTCCAGCAGCAGCAGCCCAA
TGACAAAACTAATATCCAGGGGATGACAGGAACTCCCATGGTGGCTGCCAGTCCAGAGAG
ACACGACGGTGGCTTACAGGCTAACCCAGTGGAAGTACAAAGTTACCAGCCACCTTGGAA
AGTACTGAGCGACTTCGCCTTGCAGAGTGACATAGATCAGCCTGCTTTTCAGCAACTGGT
CAATTTTTCAGAAGGAGGACCGGGCTCTAATTCCACTGGCAGTGAAGTAGCATCAATGTC
CTCTCAACTTCCAGATACACCTAACAGCATGGTAGCCAGTCCTATTGAGGCAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT