Restriction map for
pFN21AB7691
(Circular) (Six-base) MAPSORT of: pFN21AB7691.seq Check: 2436 from: 1 to: 5752
pFN21AB7691 5752 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2245 3815 5199 5467 1632
Size: 613 1570 1384 268 1917
Fragments arranged by size:
1917 1570 1384 613 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 2552 2826 1166
Size: 1386 274 4092
Fragments arranged by size:
4092 1386 274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 2935 3495 5067 2935
Size: 560 1572 3620
Fragments arranged by size:
3620 1572 560
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2360 3061 3287 3439 5548 1493
Size: 867 701 226 152 2109 1697
Fragments arranged by size:
2109 1697 867 701 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 3077 1870
Size: 73 1134 4545
Fragments arranged by size:
4545 1134 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2490 2966 3071 1391
Size: 1099 476 105 4072
Fragments arranged by size:
4072 1099 476 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2549 928
Size: 971 650 4131
Fragments arranged by size:
4131 971 650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4363 136
Size: 107 122 71 631 605 2691 1525
Fragments arranged by size:
2691 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2290 3813 4969 1790
Size: 500 1523 1156 2573
Fragments arranged by size:
2573 1523 1156 500
BmgBI CAC'GTC
Cuts at: 1318 2524 1318
Size: 1206 4546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2840 3483 4441
Size: 416 53 83 399 24 1423 643 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1423 958 643 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2118 2509 2118
Size: 391 5361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2726 4412 1239
Size: 1487 1686 2579
Fragments arranged by size:
2579 1686 1487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2387 4415 882
Size: 617 888 2028 2219
Fragments arranged by size:
2219 2028 888 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2104 4185 4583 4857 5004 5415 2104
Size: 2081 398 274 147 411 2441
Fragments arranged by size:
2441 2081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2776 2806 1178
Size: 30 165 30 1373 30 4124
Fragments arranged by size:
4124 1373 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5752
BseYI C'CCAG_C
Cuts at: 4955 5451 4955
Size: 496 5256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2269 2567 1163
Size: 573 533 298 4348
Fragments arranged by size:
4348 573 533 298
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3813 3898 4969 3813
Size: 85 1071 4596
Fragments arranged by size:
4596 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2290 2490 2966 3071 3813 3898 4969
Size: 399 500 200 476 105 742 85 1071
Cuts at: 4969 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 500 476 399 200 105
85
BspEI T'CCGG_A
Cuts at: 2104 5415 2104
Size: 3311 2441
BspHI T'CATG_A
Cuts at: 2631 2727 3641 5371 2631
Size: 96 914 1730 3012
Fragments arranged by size:
3012 1730 914 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2606 3107 844
Size: 1762 501 3489
Fragments arranged by size:
3489 1762 501
BsrBI CCG'CTC
Cuts at: 1394 1823 2292 2443 3639 1394
Size: 429 469 151 1196 3507
Fragments arranged by size:
3507 1196 469 429 151
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 2171 96
Size: 2075 3677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2590 794
Size: 1796 3956
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2020 3082 3449 3834 3851 5303 5389 5401
Size: 48 1062 367 385 17 1452 86 12
Cuts at: 5401 5747 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1062 385 367 346 86 48
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2615 2796 513
Size: 552 939 611 181 3469
Fragments arranged by size:
3469 939 611 552 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 2986 3268 4062 4082 2468
Size: 518 282 794 20 4138
Fragments arranged by size:
4138 794 518 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DraIII CAC_nnn'GTG
Cuts at: 2407 2407
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2465 2618 3199
Size: 54 1425 50 275 18 635 153 581
Cuts at: 3199 4088 5717 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 635 581 275 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2390 3682 1353
Size: 1037 1292 3423
Fragments arranged by size:
3423 1292 1037
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2485 4339 4713 4859 387
Size: 1064 138 896 1854 374 146 1280
Fragments arranged by size:
1854 1280 1064 896 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2245 2726 3815 4412 5199 5467 1239
Size: 393 613 481 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 613 597 481 393 268
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 5752
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2433 4899 1678
Size: 755 2466 2531
Fragments arranged by size:
2531 2466 755
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2202 2234 2407
Size: 32 135 32 493 32 105 32 173
Cuts at: 2407 2439 2776 2808 4393 4425 4467 4499 1373
Size: 32 337 32 1585 32 42 32 2626
Fragments arranged by size:
2626 1585 493 337 173 135 105 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 2729 2826 3710 4069 4228 518
Size: 648 1386 177 97 884 359 159 2042
Fragments arranged by size:
2042 1386 884 648 359 177 159 97
MspA1I CmG'CkG
Cuts at: 1606 2322 2483 2581 2617 3168 3845 4993 5238
Size: 716 161 98 36 551 677 1148 245
Cuts at: 5238 1606
Size: 2120
Fragments arranged by size:
2120 1148 716 677 551 245 161 98
36
NcoI C'CATG_G
Cuts at: 513 1065 2004 2796 513
Size: 552 939 792 3469
Fragments arranged by size:
3469 939 792 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2123 2130 2178 3110 4655 1132
Size: 97 894 7 48 932 1545 2229
Fragments arranged by size:
2229 1545 932 894 97 48 7
PasI CC'CwG_GG
Cuts at: 1348 2351 1348
Size: 1003 4749
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2407 1108
Size: 1299 4453
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2336 3104 830
Size: 1506 768 3478
Fragments arranged by size:
3478 1506 768
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2581 2581
Size: 5752
SacII CC_GC'GG
Cuts at: 2618 2618
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 2883 4000 5712 2883
Size: 1117 1712 2923
Fragments arranged by size:
2923 1712 1117
SfcI C'TryA_G
Cuts at: 826 1045 2332 3100 4235 4916 5107 826
Size: 219 1287 768 1135 681 191 1471
Fragments arranged by size:
1471 1287 1135 768 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 2123 3110 1132
Size: 991 987 3774
Fragments arranged by size:
3774 991 987
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2004 2295 2796 2873 3191 513
Size: 552 939 291 501 77 318 3074
Fragments arranged by size:
3074 939 552 501 318 291 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2171 2854 2881
Size: 274 80 33 51 1443 194 683 27
Cuts at: 2881 3998 5710 96
Size: 1117 1712 138
Fragments arranged by size:
1712 1443 1117 683 274 194 138 80
51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2348 2011
Size: 337 5415
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 2273 3881 2273
Size: 1608 4144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI