USP50 ORF clone pFN21AB7359

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Halotag ORF Clone pFN21AB7359

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Clone info.

Species

human

Clone Name

pFN21AB7359 Sequence (with vector) Restriction Map

Product ID

FHC02389

Flexi Clone PID

FXC02389

ORF Sequence

DNA sequence (1017 bp)
Protein sequence (339 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464334]

Gene

KIBB7359

Symbol

USP50

Description

ubiquitin specific peptidase 50

Original Clone ID

oc00573

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1017 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 339 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS53944.1 1.7e-124 98.2 USP50 CCDS61632.1 2.2e-35 37.8 USP8 CCDS10137.1 2.4e-35 37.8 USP8 CCDS58189.1 1.7e-18 34.9 USP2 CCDS8423.1 4.4e-18 35.3 USP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001394 41 335 PF00443 Peptidase C19 ProfileScan IPR001394 44 339 PS50235 Peptidase C19
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016878211 928 603158 37.8 88.8 XP_016878210 928 603158 37.8 88.8 NP_001269978 1012 603158 37.8 88.8 XP_016878207 1089 603158 37.8 88.8 XP_006720825 1089 603158 37.8 88.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7359 Download

>KIBB7359  1017 bp
ATGACTTCTCAGCCGTCTCTCCCTGCAGATGACTTCGATATCTACCACGTCCTTGCAGAG
TGCACAGATTACTATGATACCCTTCCAGTTAAGGAGGCTGATGGGAACCAGCCCCATTTT
CAGGGTGTCACTGGCTTGTGGAACTTGGGCAACACATGCTGCGTGAATGCCATCTCACAG
TGTCTCTGCAGCATCTTGCCGCTGGTGGAATACTTTCTCACCGGGAAGTATATCACCGCT
CTGCAAAAGTTTCTTCTTCCAAGCGATTGCAGTGAAGTTGCCACTGCTTTTGCCTATCTG
ATGACAGACATGTGGCTGGGAGACTCAGACTGTGTCTCACCAGAAATATTCTGGTCAGCT
CTTGGCAACCTCTACCCAGCATTTACGAAAAAGATGCAACAAGATGCTCAGGAATTCTTG
ATTTGTGTCCTAAATGAACTTCATGAAGCTCTAAAAAAGTACCACTACTCCCGGAGAAGA
TCATATGAGAAAGGATCTACTCAGAGATGCTGCAGGAAGTGGATTACCACTGAGACATCC
ATCATCACCCAGCTGTTTGAAGAGCAGCTCAATTATAGCATCGTATGTTTAAAGTGTGAG
AAATGCACCTACAAGAACGAAGTCTTCACTGTCTTCTCACTCCCCATTCCATCCAAATAT
GAATGCTCCCTTCGGGACTGTCTCCAATGTTTTTTTCAACAAGACGCACTGACCTGGAAC
AACGAAATTCACTGCTCCTTTTGTGAAACCAAGCAAGAAACTGCTGTGAGGGCCAGTATT
TCCAAAGCACCAAAAATAATTATTTTCCACCTAAAAAGGTTTGACATTCAGGGTACAACA
AAAAGGAAGCTGAGAACGGATATTCATTACCCACTCACTAACTTGGACCTCACTCCTTAT
ATTTGCTCAATTTTCCGGAAATATCCTAAATACAACCTCTGTGCAGTGGTGAACCATTTT
GGTGATTTGGATGGTGGCCACTACACTGCTTTCTGCAAGAATTCAGTCACCCAGGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB7359 Download

>KIBB7359  339 aa
MTSQPSLPADDFDIYHVLAECTDYYDTLPVKEADGNQPHFQGVTGLWNLGNTCCVNAISQ
CLCSILPLVEYFLTGKYITALQKFLLPSDCSEVATAFAYLMTDMWLGDSDCVSPEIFWSA
LGNLYPAFTKKMQQDAQEFLICVLNELHEALKKYHYSRRRSYEKGSTQRCCRKWITTETS
IITQLFEEQLNYSIVCLKCEKCTYKNEVFTVFSLPIPSKYECSLRDCLQCFFQQDALTWN
NEIHCSFCETKQETAVRASISKAPKIIIFHLKRFDIQGTTKRKLRTDIHYPLTNLDLTPY
ICSIFRKYPKYNLCAVVNHFGDLDGGHYTAFCKNSVTQA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7359 Download

>pFN21AB7359  5722 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACTTCTCAGCCGTCTCTCCCTGCAGATGACTT
CGATATCTACCACGTCCTTGCAGAGTGCACAGATTACTATGATACCCTTCCAGTTAAGGA
GGCTGATGGGAACCAGCCCCATTTTCAGGGTGTCACTGGCTTGTGGAACTTGGGCAACAC
ATGCTGCGTGAATGCCATCTCACAGTGTCTCTGCAGCATCTTGCCGCTGGTGGAATACTT
TCTCACCGGGAAGTATATCACCGCTCTGCAAAAGTTTCTTCTTCCAAGCGATTGCAGTGA
AGTTGCCACTGCTTTTGCCTATCTGATGACAGACATGTGGCTGGGAGACTCAGACTGTGT
CTCACCAGAAATATTCTGGTCAGCTCTTGGCAACCTCTACCCAGCATTTACGAAAAAGAT
GCAACAAGATGCTCAGGAATTCTTGATTTGTGTCCTAAATGAACTTCATGAAGCTCTAAA
AAAGTACCACTACTCCCGGAGAAGATCATATGAGAAAGGATCTACTCAGAGATGCTGCAG
GAAGTGGATTACCACTGAGACATCCATCATCACCCAGCTGTTTGAAGAGCAGCTCAATTA
TAGCATCGTATGTTTAAAGTGTGAGAAATGCACCTACAAGAACGAAGTCTTCACTGTCTT
CTCACTCCCCATTCCATCCAAATATGAATGCTCCCTTCGGGACTGTCTCCAATGTTTTTT
TCAACAAGACGCACTGACCTGGAACAACGAAATTCACTGCTCCTTTTGTGAAACCAAGCA
AGAAACTGCTGTGAGGGCCAGTATTTCCAAAGCACCAAAAATAATTATTTTCCACCTAAA
AAGGTTTGACATTCAGGGTACAACAAAAAGGAAGCTGAGAACGGATATTCATTACCCACT
CACTAACTTGGACCTCACTCCTTATATTTGCTCAATTTTCCGGAAATATCCTAAATACAA
CCTCTGTGCAGTGGTGAACCATTTTGGTGATTTGGATGGTGGCCACTACACTGCTTTCTG
CAAGAATTCAGTCACCCAGGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT