(Circular) (Six-base) MAPSORT of: pFN21AB7359.seq Check: 2703 from: 1 to: 5722 pFN21AB7359 5722 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3785 5169 5437 1632 Size: 2153 1384 268 1917 Fragments arranged by size: 2153 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 2313 4621 5598 1203 Size: 1110 2308 977 1327 Fragments arranged by size: 2308 1327 1110 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5722 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2216 2424 2781 1184 Size: 1032 208 357 4125 Fragments arranged by size: 4125 1032 357 208 AlwNI CAG_nnn'CTG Cuts at: 3465 5037 3465 Size: 1572 4150 ApaLI G'TGCA_C Cuts at: 2065 3779 4935 2065 Size: 1714 1156 2852 Fragments arranged by size: 2852 1714 1156 ApoI r'AATT_y Cuts at: 1493 2417 2730 3004 3031 3257 3409 5518 1493 Size: 924 313 274 27 226 152 2109 1697 Fragments arranged by size: 2109 1697 924 313 274 226 152 27 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 3047 1870 Size: 73 1104 4545 Fragments arranged by size: 4545 1104 73 BamHI G'GATC_C Cuts at: 3052 3419 3052 Size: 367 5355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3043 4498 618 Size: 325 217 627 1256 1455 1842 Fragments arranged by size: 1842 1455 1256 627 325 217 BanII G_rGCy'C Cuts at: 1391 3041 1391 Size: 1650 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2620 2629 928 Size: 971 721 9 4021 Fragments arranged by size: 4021 971 721 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2380 2414 3913 3947 1903 Size: 34 443 34 1499 34 3678 Fragments arranged by size: 3678 1499 443 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 1548 Size: 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4333 136 Size: 107 122 71 631 605 2661 1525 Fragments arranged by size: 2661 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 2069 3783 4939 1790 Size: 279 1714 1156 2573 Fragments arranged by size: 2573 1714 1156 279 BmgBI CAC'GTC Cuts at: 1318 2053 1318 Size: 735 4987 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3453 4411 442 Size: 416 53 83 399 24 2036 958 1753 Fragments arranged by size: 2036 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4382 1239 Size: 3143 2579 Bpu10I CC'TnA_GC Cuts at: 2412 2412 Size: 5722 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3847 4712 5010 3847 Size: 865 298 4559 Fragments arranged by size: 4559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4385 882 Size: 617 2886 2219 Fragments arranged by size: 2886 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 5716 5716 Size: 5722 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2919 4155 4553 4827 4974 5385 2919 Size: 1236 398 274 147 411 3256 Fragments arranged by size: 3256 1236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2456 2486 1178 Size: 30 165 30 1053 30 4414 Fragments arranged by size: 4414 1053 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5722 BseYI C'CCAG_C Cuts at: 2320 2380 2553 4925 5421 2320 Size: 60 173 2372 496 2621 Fragments arranged by size: 2621 2372 496 173 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2967 1163 Size: 573 1231 3918 Fragments arranged by size: 3918 1231 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3172 3933 4961 1540 Size: 275 186 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2069 3783 3868 4939 2069 Size: 1714 85 1071 2852 Fragments arranged by size: 2852 1714 1071 85 BsmI GAATG_Cn' Cuts at: 2176 2672 3233 3326 2176 Size: 496 561 93 4572 Fragments arranged by size: 4572 561 496 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2025 2025 Size: 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 3041 3783 3868 4939 1391 Size: 399 279 972 742 85 1071 2174 Fragments arranged by size: 2174 1071 972 742 399 279 85 BspEI T'CCGG_A Cuts at: 2919 5385 2919 Size: 2466 3256 BspHI T'CATG_A Cuts at: 2446 3611 5341 2446 Size: 1165 1730 2827 Fragments arranged by size: 2827 1730 1165 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 844 Size: 2233 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2243 3609 1394 Size: 429 420 1366 3507 Fragments arranged by size: 3507 1366 429 420 BsrDI GCAATG_nn' Cuts at: 65 1248 4217 4391 65 Size: 1183 2969 174 1396 Fragments arranged by size: 2969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4366 4553 1700 Size: 110 2556 187 2869 Fragments arranged by size: 2869 2556 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 2160 794 Size: 1366 4356 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2498 3052 3419 3804 3821 5273 5359 5371 Size: 526 554 367 385 17 1452 86 12 Cuts at: 5371 5717 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 554 526 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5170 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 2281 2286 2734 2955 2988 3238 4032 4052 2281 Size: 5 448 221 33 250 794 20 3951 Fragments arranged by size: 3951 794 448 250 221 33 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2275 2310 529 Size: 35 1711 35 3941 Fragments arranged by size: 3941 1711 35 35 DraI TTT'AAA Cuts at: 2595 3026 2595 Size: 431 5291 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2980 3169 4058 Size: 54 1425 50 275 18 1150 189 889 Cuts at: 4058 5687 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1150 889 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2559 3652 1353 Size: 1206 1093 3423 Fragments arranged by size: 3423 1206 1093 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3785 4382 5169 5437 1239 Size: 393 2153 597 787 268 1524 Fragments arranged by size: 2153 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2417 3004 3031 3409 2417 Size: 587 27 378 4730 Fragments arranged by size: 4730 587 378 27 EcoRV GAT'ATC Cuts at: 2044 2044 Size: 5722 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 4869 1678 Size: 3191 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 2483 2515 4363 Size: 32 135 32 752 32 127 32 1848 Cuts at: 4363 4395 4437 4469 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1848 752 135 127 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3066 3312 669 Size: 988 281 1128 246 3079 Fragments arranged by size: 3079 1128 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5722 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4836 5020 1305 Size: 3531 184 2007 Fragments arranged by size: 3531 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2982 10 Size: 54 1425 343 1150 2750 Fragments arranged by size: 2750 1425 1150 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2964 3680 4039 4198 518 Size: 648 1798 716 359 159 2042 Fragments arranged by size: 2042 1798 716 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 2557 3138 3815 4963 5208 1606 Size: 600 351 581 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 600 581 351 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5170 NdeI CA'TA_TG Cuts at: 387 2488 387 Size: 2101 3621 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5722 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 2163 2317 3080 4625 1132 Size: 97 934 154 763 1545 2229 Fragments arranged by size: 2229 1545 934 763 154 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 2313 4621 2313 Size: 2308 3414 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5722 PfoI T'CCnGG_A Cuts at: 2474 2474 Size: 5722 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PstI C_TGCA'G Cuts at: 830 2032 2195 2519 3074 830 Size: 1202 163 324 555 3478 Fragments arranged by size: 3478 1202 555 324 163 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 PvuII CAG'CTG Cuts at: 2557 2557 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SapI GCTCTTCn'nnn_ Cuts at: 2559 2559 Size: 5722 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 2028 2191 2515 3070 4205 4886 5077 Size: 219 983 163 324 555 1135 681 191 Cuts at: 5077 826 Size: 1471 Fragments arranged by size: 1471 1135 983 681 555 324 219 191 163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3826 4727 4989 820 Size: 197 926 1883 901 262 1553 Fragments arranged by size: 1883 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 2352 3646 5 Size: 47 2300 1294 2081 Fragments arranged by size: 2300 2081 1294 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5722 StyI C'CwwG_G Cuts at: 513 1065 3161 513 Size: 552 2096 3074 Fragments arranged by size: 3074 2096 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2567 3029 3749 3766 3919 4104 4549 Size: 1255 987 462 720 17 153 185 445 Cuts at: 4549 325 Size: 1498 Fragments arranged by size: 1498 1255 987 720 462 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3968 5680 96 Size: 274 80 33 51 1443 1991 1712 138 Fragments arranged by size: 1991 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5682 5714 5682 Size: 32 5690 Tth111I GACn'n_nGTC Cuts at: 1316 2336 1316 Size: 1020 4702 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2352 2352 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5722 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 2217 3851 2217 Size: 1634 4088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI