GJB5 ORF clone pFN21AB7114

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Halotag ORF Clone pFN21AB7114

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Clone info.

Species

human

Clone Name

pFN21AB7114 Sequence (with vector) Restriction Map

Product ID

FHC02259

Flexi Clone PID

FXC02259

ORF Sequence

DNA sequence (819 bp)
Protein sequence (273 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463669]

Gene

KIBB7114

Symbol

GJB5

Alias : CX31.1

Description

gap junction protein, beta 5, 31.1kDa

Original Clone ID

cp01119

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 819 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 273 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS382.1 1.1e-131 100.0 GJB5 CCDS383.1 5.8e-74 67.7 GJB4 CCDS384.1 1.7e-69 57.1 GJB3 CCDS5008.1 6.9e-59 55.2 GJB7 CCDS9291.1 6e-56 46.8 GJB6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000500 20 44 PR00206 Connexin IPR000500 51 73 PR00206 Connexin IPR000500 76 96 PR00206 Connexin IPR000500 127 153 PR00206 Connexin IPR002270 139 146 PR01141 Gap junction beta-4 protein (Cx31.1) IPR000500 164 184 PR00206 Connexin IPR000500 185 208 PR00206 Connexin IPR002270 213 224 PR01141 Gap junction beta-4 protein (Cx31.1) IPR002270 238 249 PR01141 Gap junction beta-4 protein (Cx31.1) IPR002270 250 260 PR01141 Gap junction beta-4 protein (Cx31.1) HMMPfam IPR013092 2 107 PF00029 Connexin IPR019570 142 208 PF10582 Gap junction protein HMMSmart IPR013092 42 75 SM00037 Connexin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 19 AFGRIWLSLVFIFRVLVYLVTAE 41 SECONDARY 23 2 76 LWALQLILVTCPSLLVVMHVAYR 98 PRIMARY 23 3 128 WWTYVCSLVFKASVDIAFLYVFH 150 PRIMARY 23 4 186 FTLFMVATAAICILLNLVELIYL 208 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005270808 273 604493 100.0 100.0 NP_005259 273 604493 100.0 100.0 NP_694944 266 133200,605425 67.7 82.8 XP_011538981 266 133200,605425 67.7 82.8 NP_001005752 270 133200,220290,603324,612644 57.1 87.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7114 Download

>KIBB7114  819 bp
ATGAACTGGAGTATCTTTGAGGGACTCCTGAGTGGGGTCAACAAGTACTCCACAGCCTTT
GGGCGCATCTGGCTGTCTCTGGTCTTCATCTTCCGCGTGCTGGTGTACCTGGTGACGGCC
GAGCGTGTGTGGAGTGATGACCACAAGGACTTCGACTGCAATACTCGCCAGCCCGGCTGC
TCCAACGTCTGCTTTGATGAGTTCTTCCCTGTGTCCCATGTGCGCCTCTGGGCCCTGCAG
CTTATCCTGGTGACATGCCCCTCACTGCTCGTGGTCATGCACGTGGCCTACCGGGAGGTT
CAGGAGAAGAGGCACCGAGAAGCCCATGGGGAGAACAGTGGGCGCCTCTACCTGAACCCC
GGCAAGAAGCGGGGTGGGCTCTGGTGGACATATGTCTGCAGCCTAGTGTTCAAGGCGAGC
GTGGACATCGCCTTTCTCTATGTGTTCCACTCATTCTACCCCAAATATATCCTCCCTCCT
GTGGTCAAGTGCCACGCAGATCCATGTCCCAATATAGTGGACTGCTTCATCTCCAAGCCC
TCAGAGAAGAACATTTTCACCCTCTTCATGGTGGCCACAGCTGCCATCTGCATCCTGCTC
AACCTCGTGGAGCTCATCTACCTGGTGAGCAAGAGATGCCACGAGTGCCTGGCAGCAAGG
AAAGCTCAAGCCATGTGCACAGGTCATCACCCCCACGGTACCACCTCTTCCTGCAAACAA
GACGACCTCCTTTCGGGTGACCTCATCTTTCTGGGCTCAGACAGTCATCCTCCTCTCTTA
CCAGACCGCCCCCGAGACCATGTGAAGAAAACCATCTTG

ORF Protein

Cloned ORF protein sequence for pFN21AB7114 Download

>KIBB7114  273 aa
MNWSIFEGLLSGVNKYSTAFGRIWLSLVFIFRVLVYLVTAERVWSDDHKDFDCNTRQPGC
SNVCFDEFFPVSHVRLWALQLILVTCPSLLVVMHVAYREVQEKRHREAHGENSGRLYLNP
GKKRGGLWWTYVCSLVFKASVDIAFLYVFHSFYPKYILPPVVKCHADPCPNIVDCFISKP
SEKNIFTLFMVATAAICILLNLVELIYLVSKRCHECLAARKAQAMCTGHHPHGTTSSCKQ
DDLLSGDLIFLGSDSHPPLLPDRPRDHVKKTIL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7114 Download

>pFN21AB7114  5524 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACTGGAGTATCTTTGAGGGACTCCTGAGTGG
GGTCAACAAGTACTCCACAGCCTTTGGGCGCATCTGGCTGTCTCTGGTCTTCATCTTCCG
CGTGCTGGTGTACCTGGTGACGGCCGAGCGTGTGTGGAGTGATGACCACAAGGACTTCGA
CTGCAATACTCGCCAGCCCGGCTGCTCCAACGTCTGCTTTGATGAGTTCTTCCCTGTGTC
CCATGTGCGCCTCTGGGCCCTGCAGCTTATCCTGGTGACATGCCCCTCACTGCTCGTGGT
CATGCACGTGGCCTACCGGGAGGTTCAGGAGAAGAGGCACCGAGAAGCCCATGGGGAGAA
CAGTGGGCGCCTCTACCTGAACCCCGGCAAGAAGCGGGGTGGGCTCTGGTGGACATATGT
CTGCAGCCTAGTGTTCAAGGCGAGCGTGGACATCGCCTTTCTCTATGTGTTCCACTCATT
CTACCCCAAATATATCCTCCCTCCTGTGGTCAAGTGCCACGCAGATCCATGTCCCAATAT
AGTGGACTGCTTCATCTCCAAGCCCTCAGAGAAGAACATTTTCACCCTCTTCATGGTGGC
CACAGCTGCCATCTGCATCCTGCTCAACCTCGTGGAGCTCATCTACCTGGTGAGCAAGAG
ATGCCACGAGTGCCTGGCAGCAAGGAAAGCTCAAGCCATGTGCACAGGTCATCACCCCCA
CGGTACCACCTCTTCCTGCAAACAAGACGACCTCCTTTCGGGTGACCTCATCTTTCTGGG
CTCAGACAGTCATCCTCCTCTCTTACCAGACCGCCCCCGAGACCATGTGAAGAAAACCAT
CTTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT