(Circular) (Six-base) MAPSORT of: pFN21AB7114.seq Check: 6714 from: 1 to: 5524 pFN21AB7114 5524 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2867 1937 Size: 930 4594 Acc65I G'GTAC_C Cuts at: 2702 2845 2702 Size: 143 5381 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3587 4971 5239 1632 Size: 1955 1384 268 1917 Fragments arranged by size: 1955 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 4423 5400 1203 Size: 3220 977 1327 Fragments arranged by size: 3220 1327 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 3267 4839 3267 Size: 1572 3952 ApaI G_GGCC'C Cuts at: 2239 2239 Size: 5524 ApaLI G'TGCA_C Cuts at: 2680 3581 4737 2680 Size: 901 1156 3467 Fragments arranged by size: 3467 1156 901 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2796 2849 1870 Size: 73 853 53 4545 Fragments arranged by size: 4545 853 73 53 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1999 2032 1999 Size: 33 5491 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2316 2346 2702 2845 4300 Size: 325 217 627 529 30 356 143 1455 Cuts at: 4300 618 Size: 1842 Fragments arranged by size: 1842 1455 627 529 356 325 217 143 30 BanII G_rGCy'C Cuts at: 1391 2239 2385 2619 2762 2843 1391 Size: 848 146 234 143 81 4072 Fragments arranged by size: 4072 848 234 146 143 81 BbeI G_GCGC'C Cuts at: 2350 2350 Size: 5524 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2080 928 Size: 971 181 4372 Fragments arranged by size: 4372 971 181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3715 3749 1903 Size: 34 1778 34 3678 Fragments arranged by size: 3678 1778 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2178 4135 136 Size: 107 122 71 631 605 506 1957 1525 Fragments arranged by size: 1957 1525 631 605 506 122 107 71 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 2239 2684 3585 4741 1790 Size: 449 445 901 1156 2573 Fragments arranged by size: 2573 1156 901 449 445 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3255 4213 442 Size: 416 53 83 399 24 1838 958 1753 Fragments arranged by size: 1838 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 4184 1239 Size: 793 2152 2579 Fragments arranged by size: 2579 2152 793 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2655 3649 4514 4812 2655 Size: 994 865 298 3367 Fragments arranged by size: 3367 994 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2793 4187 882 Size: 617 1294 1394 2219 Fragments arranged by size: 2219 1394 1294 617 BsaAI yAC'GTr Cuts at: 493 1204 2287 3315 493 Size: 711 1083 1028 2702 Fragments arranged by size: 2702 1083 1028 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2347 3331 3713 4348 Size: 53 83 186 936 814 984 382 635 Cuts at: 4348 275 Size: 1451 Fragments arranged by size: 1451 984 936 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3957 4355 4629 4776 5187 3957 Size: 398 274 147 411 4294 Fragments arranged by size: 4294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 1178 Size: 30 165 30 633 30 4636 Fragments arranged by size: 4636 633 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2766 1186 Size: 1580 3944 BseYI C'CCAG_C Cuts at: 4727 5223 4727 Size: 496 5028 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4951 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2124 2974 3735 4763 1540 Size: 275 186 123 850 761 1028 2301 Fragments arranged by size: 2301 1028 850 761 275 186 123 BsiHKAI G_wGCw'C Cuts at: 2619 2684 3585 3670 4741 2619 Size: 65 901 85 1071 3402 Fragments arranged by size: 3402 1071 901 85 65 BsmI GAATG_Cn' Cuts at: 3035 3128 3035 Size: 93 5431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2385 2619 2684 2762 2843 3585 Size: 399 449 146 234 65 78 81 742 Cuts at: 3585 3670 4741 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 399 234 146 85 81 78 65 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2879 844 Size: 2035 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3411 1394 Size: 429 1588 3507 Fragments arranged by size: 3507 1588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4168 4355 1700 Size: 110 2358 187 2869 Fragments arranged by size: 2869 2358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5524 BssSI C'ACGA_G Cuts at: 2273 2609 2645 3584 4596 2273 Size: 336 36 939 1012 3201 Fragments arranged by size: 3201 1012 939 336 36 BstAPI GCAn_nnn'nTGC Cuts at: 794 2640 794 Size: 1846 3678 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 2741 1184 Size: 1557 3967 BstXI CCAn_nnnn'nTGG Cuts at: 2521 2521 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2503 2854 3221 3606 3623 5075 5161 5173 Size: 531 351 367 385 17 1452 86 12 Cuts at: 5173 5519 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 531 385 367 351 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2329 2698 513 Size: 552 1264 369 3339 Fragments arranged by size: 3339 1264 552 369 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2416 487 Size: 683 56 1190 3595 Fragments arranged by size: 3595 1190 683 56 BtsI GCAGTG_nn' Cuts at: 2267 3040 3834 3854 2267 Size: 773 794 20 3937 Fragments arranged by size: 3937 794 773 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2044 2079 529 Size: 35 1480 35 3974 Fragments arranged by size: 3974 1480 35 35 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2577 2971 Size: 54 1425 50 275 18 291 456 394 Cuts at: 2971 3860 5489 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 456 394 291 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2121 2971 1537 Size: 275 309 850 4090 Fragments arranged by size: 4090 850 309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2306 2573 2716 3454 1353 Size: 953 267 143 738 3423 Fragments arranged by size: 3423 953 738 267 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4111 4485 4631 387 Size: 1064 138 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2617 2617 Size: 5524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 3587 4184 4971 5239 1239 Size: 393 400 1555 597 787 268 1524 Fragments arranged by size: 1555 1524 787 597 400 393 268 EcoO109I rG'GnC_Cy Cuts at: 2236 2236 Size: 5524 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 2350 4671 1678 Size: 672 2321 2531 Fragments arranged by size: 2531 2321 672 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373 Size: 32 135 32 2593 32 42 32 2626 Fragments arranged by size: 2626 2593 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2044 2868 3114 669 Size: 988 281 106 824 246 3079 Fragments arranged by size: 3079 988 824 281 246 106 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KasI G'GCGC_C Cuts at: 2346 2346 Size: 5524 KpnI G_GTAC'C Cuts at: 2706 2849 2706 Size: 143 5381 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 5400 5400 Size: 5524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 4638 4822 1305 Size: 906 2427 184 2007 Fragments arranged by size: 2427 2007 906 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 10 Size: 54 1425 343 747 2955 Fragments arranged by size: 2955 1425 747 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 3482 3841 4000 518 Size: 648 1119 1197 359 159 2042 Fragments arranged by size: 2042 1197 1119 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2585 2940 3617 4765 5010 1606 Size: 979 355 677 1148 245 2120 Fragments arranged by size: 2120 1148 979 677 355 245 NarI GG'CG_CC Cuts at: 2347 2347 Size: 5524 NcoI C'CATG_G Cuts at: 513 1065 2329 513 Size: 552 1264 3708 Fragments arranged by size: 3708 1264 552 NdeI CA'TA_TG Cuts at: 387 2395 387 Size: 2008 3516 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2262 2882 4427 1132 Size: 97 1033 620 1545 2229 Fragments arranged by size: 2229 1545 1033 620 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5524 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4622 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PmlI CAC'GTG Cuts at: 2287 2287 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2396 1613 Size: 783 4741 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PspOMI G'GGCC_C Cuts at: 2235 2235 Size: 5524 PstI C_TGCA'G Cuts at: 830 2244 2405 2876 830 Size: 1414 161 471 3478 Fragments arranged by size: 3478 1414 471 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 PvuII CAG'CTG Cuts at: 2585 2585 Size: 5524 SacI G_AGCT'C Cuts at: 2619 2619 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 2051 3772 5484 2051 Size: 1721 1712 2091 Fragments arranged by size: 2091 1721 1712 SexAI A'CCwGG_T Cuts at: 2112 2625 2112 Size: 513 5011 SfcI C'TryA_G Cuts at: 826 1045 2240 2401 2872 4007 4688 4879 826 Size: 219 1195 161 471 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 681 471 219 191 161 SfoI GGC'GCC Cuts at: 2348 2348 Size: 5524 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2851 1872 Size: 979 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2670 3628 4529 4791 820 Size: 197 926 727 958 901 262 1553 Fragments arranged by size: 1553 958 926 901 727 262 197 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2882 1132 Size: 1750 3774 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5524 StyI C'CwwG_G Cuts at: 513 1065 2329 2963 513 Size: 552 1264 634 3074 Fragments arranged by size: 3074 1264 634 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3551 3568 3721 3906 4351 325 Size: 1255 1971 17 153 185 445 1498 Fragments arranged by size: 1971 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2049 3770 5482 Size: 274 80 33 51 1443 72 1721 1712 Cuts at: 5482 96 Size: 138 Fragments arranged by size: 1721 1712 1443 274 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 5492 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2849 1870 Size: 979 4545 XmnI GAAnn'nnTTC Cuts at: 2558 3653 2558 Size: 1095 4429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI