WFIKKN2 ORF clone pFN21AB6013

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Halotag ORF Clone pFN21AB6013

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Clone info.

Species

human

Clone Name

pFN21AB6013 Sequence (with vector) Restriction Map

Product ID

FHC10477

Flexi Clone PID

FXC10477

Original Clone PID

ORK10477

ORF Sequence

DNA sequence (1728 bp)
Protein sequence (576 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527284]

Gene

KIBB6013

Symbol

WFIKKN2

Alias : GASP-1, WFDC20B, WFIKKNRP, hGASP-1

Description

WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2

Original Clone ID

fk05082

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1728 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 576 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11575.1 1.4e-168 100.0 WFIKKN2 CCDS82159.1 3e-139 100.0 WFIKKN2 CCDS10414.1 2.6e-61 56.1 WFIKKN1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002223 383 397 PR00759 Proteinase inhibitor I2 IPR002223 411 421 PR00759 Proteinase inhibitor I2 IPR002223 421 436 PR00759 Proteinase inhibitor I2 HMMPfam IPR008197 42 90 PF00095 Whey acidic protein IPR011497 138 168 PF07648 Protease inhibitor IPR013098 214 304 PF07679 Immunoglobulin I-set IPR002223 328 379 PF00014 Proteinase inhibitor I2 IPR002223 385 436 PF00014 Proteinase inhibitor I2 IPR018933 457 559 PF01759 Netrin module HMMSmart IPR008197 42 92 SM00217 Whey acidic protein IPR003599 216 305 SM00409 Immunoglobulin subtype IPR003598 222 294 SM00408 Immunoglobulin subtype 2 IPR002223 326 379 SM00131 Proteinase inhibitor I2 IPR002223 384 437 SM00131 Proteinase inhibitor I2 ProfileScan IPR008197 39 92 PS51390 Whey acidic protein NULL 126 177 PS51465 NULL IPR007110 210 303 PS50835 Immunoglobulin-like IPR002223 328 378 PS50279 Proteinase inhibitor I2 IPR002223 386 436 PS50279 Proteinase inhibitor I2 IPR001134 445 566 PS50189 Netrin domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_783165 576 610895 100.0 100.0 NP_001317270 483 610895 100.0 83.9 NP_444514 548 608021 56.1 96.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6013 Download

>KIBB6013  1728 bp
ATGTGGGCCCCAAGGTGTCGCCGGTTCTGGTCTCGCTGGGAGCAGGTGGCAGCGCTGCTG
CTGCTGCTGCTACTGCTCGGGGTGCCCCCGCGAAGCCTGGCGCTGCCGCCCATCCGCTAT
TCCCACGCCGGCATCTGCCCCAACGACATGAATCCCAACCTCTGGGTGGACGCACAGAGC
ACCTGCAGGCGGGAGTGTGAGACGGACCAGGAGTGTGAGACCTATGAGAAGTGCTGCCCC
AACGTATGTGGGACCAAGAGCTGCGTGGCGGCCCGCTACATGGACGTGAAAGGGAAGAAG
GGCCCAGTGGGCATGCCCAAGGAGGCCACATGTGACCACTTCATGTGTCTGCAGCAGGGC
TCTGAGTGTGACATCTGGGATGGCCAGCCCGTGTGTAAGTGCAAAGACCGCTGTGAGAAG
GAGCCCAGCTTTACCTGCGCCTCGGACGGCCTCACCTACTATAACCGCTGCTACATGGAT
GCCGAGGCCTGCTCCAAAGGCATCACACTGGCCGTTGTAACCTGCCGCTATCACTTCACC
TGGCCCAACACCAGCCCCCCACCACCTGAGACCACCATGCACCCCACCACAGCCTCCCCA
GAGACCCCTGAGCTGGACATGGCGGCCCCTGCGCTGCTCAACAACCCTGTGCACCAGTCG
GTCACCATGGGTGAGACAGTGAGTTTCCTCTGTGATGTGGTGGGCCGGCCCCGGCCTGAG
ATCACCTGGGAGAAGCAGTTGGAGGATCGGGAGAATGTGGTCATGCGGCCCAACCATGTG
CGTGGCAACGTGGTGGTCACCAACATTGCCCAGCTGGTCATCTATAACGCCCAGCTGCAG
GATGCTGGGATCTACACCTGCACGGCCCGGAACGTGGCTGGGGTCCTGAGGGCTGATTTC
CCGCTGTCGGTGGTCAGGGGTCATCAGGCTGCAGCCACCTCAGAGAGCAGCCCCAATGGC
ACGGCTTTCCCGGCGGCCGAGTGCCTGAAGCCCCCAGACAGTGAGGACTGTGGCGAAGAG
CAGACCCGCTGGCACTTCGATGCCCAGGCCAACAACTGCCTGACCTTCACCTTCGGCCAC
TGCCACCGTAACCTCAACCACTTTGAGACCTATGAGGCCTGCATGCTGGCCTGCATGAGC
GGGCCGCTGGCCGCGTGCAGCCTGCCCGCCCTGCAGGGGCCCTGCAAAGCCTACGCGCCT
CGCTGGGCTTACAACAGCCAGACGGGCCAGTGCCAGTCCTTTGTCTATGGTGGCTGCGAG
GGCAATGGCAACAACTTTGAGAGCCGTGAGGCCTGTGAGGAGTCGTGCCCCTTCCCCAGG
GGGAACCAGCGCTGTCGGGCCTGCAAGCCTCGGCAGAAGCTCGTTACCAGCTTCTGTCGC
AGCGACTTTGTCATCCTGGGCCGAGTCTCTGAGCTGACCGAGGAGCCTGACTCGGGCCGC
GCCCTGGTGACTGTGGATGAGGTCCTAAAGGATGAGAAAATGGGCCTCAAGTTCCTGGGC
CAGGAGCCATTGGAGGTCACTCTGCTTCACGTGGACTGGGCATGCCCCTGCCCCAACGTG
ACCGTGAGCGAGATGCCGCTCATCATCATGGGGGAGGTGGACGGCGGCATGGCCATGCTG
CGCCCCGATAGCTTTGTGGGCGCATCGAGTGCCCGCCGGGTCAGGAAGCTTCGTGAGGTC
ATGCACAAGAAGACCTGTGACGTCCTCAAGGAGTTTCTTGGCTTGCAC

ORF Protein

Cloned ORF protein sequence for pFN21AB6013 Download

>KIBB6013  576 aa
MWAPRCRRFWSRWEQVAALLLLLLLLGVPPRSLALPPIRYSHAGICPNDMNPNLWVDAQS
TCRRECETDQECETYEKCCPNVCGTKSCVAARYMDVKGKKGPVGMPKEATCDHFMCLQQG
SECDIWDGQPVCKCKDRCEKEPSFTCASDGLTYYNRCYMDAEACSKGITLAVVTCRYHFT
WPNTSPPPPETTMHPTTASPETPELDMAAPALLNNPVHQSVTMGETVSFLCDVVGRPRPE
ITWEKQLEDRENVVMRPNHVRGNVVVTNIAQLVIYNAQLQDAGIYTCTARNVAGVLRADF
PLSVVRGHQAAATSESSPNGTAFPAAECLKPPDSEDCGEEQTRWHFDAQANNCLTFTFGH
CHRNLNHFETYEACMLACMSGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCE
GNGNNFESREACEESCPFPRGNQRCRACKPRQKLVTSFCRSDFVILGRVSELTEEPDSGR
ALVTVDEVLKDEKMGLKFLGQEPLEVTLLHVDWACPCPNVTVSEMPLIIMGEVDGGMAML
RPDSFVGASSARRVRKLREVMHKKTCDVLKEFLGLH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6013 Download

>pFN21AB6013  6433 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGGGCCCCAAGGTGTCGCCGGTTCTGGTCTCG
CTGGGAGCAGGTGGCAGCGCTGCTGCTGCTGCTGCTACTGCTCGGGGTGCCCCCGCGAAG
CCTGGCGCTGCCGCCCATCCGCTATTCCCACGCCGGCATCTGCCCCAACGACATGAATCC
CAACCTCTGGGTGGACGCACAGAGCACCTGCAGGCGGGAGTGTGAGACGGACCAGGAGTG
TGAGACCTATGAGAAGTGCTGCCCCAACGTATGTGGGACCAAGAGCTGCGTGGCGGCCCG
CTACATGGACGTGAAAGGGAAGAAGGGCCCAGTGGGCATGCCCAAGGAGGCCACATGTGA
CCACTTCATGTGTCTGCAGCAGGGCTCTGAGTGTGACATCTGGGATGGCCAGCCCGTGTG
TAAGTGCAAAGACCGCTGTGAGAAGGAGCCCAGCTTTACCTGCGCCTCGGACGGCCTCAC
CTACTATAACCGCTGCTACATGGATGCCGAGGCCTGCTCCAAAGGCATCACACTGGCCGT
TGTAACCTGCCGCTATCACTTCACCTGGCCCAACACCAGCCCCCCACCACCTGAGACCAC
CATGCACCCCACCACAGCCTCCCCAGAGACCCCTGAGCTGGACATGGCGGCCCCTGCGCT
GCTCAACAACCCTGTGCACCAGTCGGTCACCATGGGTGAGACAGTGAGTTTCCTCTGTGA
TGTGGTGGGCCGGCCCCGGCCTGAGATCACCTGGGAGAAGCAGTTGGAGGATCGGGAGAA
TGTGGTCATGCGGCCCAACCATGTGCGTGGCAACGTGGTGGTCACCAACATTGCCCAGCT
GGTCATCTATAACGCCCAGCTGCAGGATGCTGGGATCTACACCTGCACGGCCCGGAACGT
GGCTGGGGTCCTGAGGGCTGATTTCCCGCTGTCGGTGGTCAGGGGTCATCAGGCTGCAGC
CACCTCAGAGAGCAGCCCCAATGGCACGGCTTTCCCGGCGGCCGAGTGCCTGAAGCCCCC
AGACAGTGAGGACTGTGGCGAAGAGCAGACCCGCTGGCACTTCGATGCCCAGGCCAACAA
CTGCCTGACCTTCACCTTCGGCCACTGCCACCGTAACCTCAACCACTTTGAGACCTATGA
GGCCTGCATGCTGGCCTGCATGAGCGGGCCGCTGGCCGCGTGCAGCCTGCCCGCCCTGCA
GGGGCCCTGCAAAGCCTACGCGCCTCGCTGGGCTTACAACAGCCAGACGGGCCAGTGCCA
GTCCTTTGTCTATGGTGGCTGCGAGGGCAATGGCAACAACTTTGAGAGCCGTGAGGCCTG
TGAGGAGTCGTGCCCCTTCCCCAGGGGGAACCAGCGCTGTCGGGCCTGCAAGCCTCGGCA
GAAGCTCGTTACCAGCTTCTGTCGCAGCGACTTTGTCATCCTGGGCCGAGTCTCTGAGCT
GACCGAGGAGCCTGACTCGGGCCGCGCCCTGGTGACTGTGGATGAGGTCCTAAAGGATGA
GAAAATGGGCCTCAAGTTCCTGGGCCAGGAGCCATTGGAGGTCACTCTGCTTCACGTGGA
CTGGGCATGCCCCTGCCCCAACGTGACCGTGAGCGAGATGCCGCTCATCATCATGGGGGA
GGTGGACGGCGGCATGGCCATGCTGCGCCCCGATAGCTTTGTGGGCGCATCGAGTGCCCG
CCGGGTCAGGAAGCTTCGTGAGGTCATGCACAAGAAGACCTGTGACGTCCTCAAGGAGTT
TCTTGGCTTGCACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT