(Circular) (Six-base) MAPSORT of: pFN21AB6013.seq Check: 6391 from: 1 to: 6433 pFN21AB6013 6433 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2038 2195 2870 2038 Size: 157 675 5601 Fragments arranged by size: 5601 675 157 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3708 4243 278 Size: 53 83 186 936 2172 535 2468 Fragments arranged by size: 2468 2172 936 535 186 83 53 AccI GT'mk_AC Cuts at: 1937 3776 1937 Size: 1839 4594 Acc65I G'GTAC_C Cuts at: 3754 3754 Size: 6433 AclI AA'CG_TT Cuts at: 1563 4560 4933 1563 Size: 2997 373 3063 Fragments arranged by size: 3063 2997 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3011 4496 5880 6148 1632 Size: 1379 1485 1384 268 1917 Fragments arranged by size: 1917 1485 1384 1379 268 AfeI AGC'GCT Cuts at: 2058 3335 2058 Size: 1277 5156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6236 AflIII A'CryG_T Cuts at: 1203 2333 5332 6309 1203 Size: 1130 2999 977 1327 Fragments arranged by size: 2999 1327 1130 977 AgeI A'CCGG_T Cuts at: 5264 5264 Size: 6433 AhdI GACnn_n'nnGTC Cuts at: 5162 5162 Size: 6433 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6433 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3060 3568 3635 1184 Size: 1876 508 67 3982 Fragments arranged by size: 3982 1876 508 67 AlwNI CAG_nnn'CTG Cuts at: 3378 4176 5748 3378 Size: 798 1572 4063 Fragments arranged by size: 4063 1572 798 ApaI G_GGCC'C Cuts at: 2014 2309 3186 2014 Size: 295 877 5261 Fragments arranged by size: 5261 877 295 ApaLI G'TGCA_C Cuts at: 2654 4490 5646 2654 Size: 1836 1156 3441 Fragments arranged by size: 3441 1836 1156 ApoI r'AATT_y Cuts at: 1493 3742 3968 4120 6229 1493 Size: 2249 226 152 2109 1697 Fragments arranged by size: 2249 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4987 160 Size: 872 3955 1606 Fragments arranged by size: 3955 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 AvaI C'yCGr_G Cuts at: 1870 1943 2081 3436 3758 1870 Size: 73 138 1355 322 4545 Fragments arranged by size: 4545 1355 322 138 73 BamHI G'GATC_C Cuts at: 3763 4130 3763 Size: 367 6066 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 3754 5209 618 Size: 325 217 627 299 1668 1455 1842 Fragments arranged by size: 1842 1668 1455 627 325 299 217 BanII G_rGCy'C Cuts at: 1391 2014 2309 2366 2430 3186 3752 1391 Size: 623 295 57 64 756 566 4072 Fragments arranged by size: 4072 756 623 566 295 64 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3701 928 Size: 971 1802 3660 Fragments arranged by size: 3660 1802 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4624 4658 1903 Size: 34 2687 34 3678 Fragments arranged by size: 3678 2687 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4325 5535 4325 Size: 1210 5223 BclI T'GATC_A Cuts at: 1548 1548 Size: 6433 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2313 2961 Size: 107 122 71 631 605 337 304 648 Cuts at: 2961 3228 5044 136 Size: 267 1816 1525 Fragments arranged by size: 1816 1525 648 631 605 337 304 267 122 107 71 BglII A'GATC_T Cuts at: 6428 6428 Size: 6433 BlpI GC'TnA_GC Cuts at: 3850 3850 Size: 6433 Bme1580I G_kGCm'C Cuts at: 1790 2014 2091 2309 2658 3186 3314 3658 4494 Size: 224 77 218 349 528 128 344 836 Cuts at: 4494 5650 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 836 528 349 344 224 218 128 77 BmgBI CAC'GTC Cuts at: 1318 2290 1318 Size: 972 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2303 3550 4164 Size: 416 53 83 399 24 886 1247 614 Cuts at: 4164 5122 442 Size: 958 1753 Fragments arranged by size: 1753 1247 958 886 614 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6433 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2512 3451 2512 Size: 939 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5093 1239 Size: 3854 2579 Bpu10I CC'TnA_GC Cuts at: 2613 2613 Size: 6433 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3476 3695 4558 5423 5721 3476 Size: 219 863 865 298 4188 Fragments arranged by size: 4188 865 863 298 219 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2040 2216 2567 2600 3104 5096 882 Size: 617 541 176 351 33 504 1992 2219 Fragments arranged by size: 2219 1992 617 541 504 351 176 33 BsaAI yAC'GTr Cuts at: 493 1204 3535 4224 493 Size: 711 2331 689 2702 Fragments arranged by size: 2702 2331 711 689 BsaBI GATnn'nnATC Cuts at: 6427 6427 Size: 6433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3705 4240 4622 5257 Size: 53 83 186 936 2172 535 382 635 Cuts at: 5257 275 Size: 1451 Fragments arranged by size: 2172 1451 936 635 535 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4866 5264 5538 5685 6096 4866 Size: 398 274 147 411 5203 Fragments arranged by size: 5203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2726 2756 1178 Size: 30 165 30 1323 30 4855 Fragments arranged by size: 4855 1323 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3317 3440 1186 Size: 2131 123 4179 Fragments arranged by size: 4179 2131 123 BseYI C'CCAG_C Cuts at: 2040 2429 2814 2835 2849 2882 3207 5636 6132 Size: 389 385 21 14 33 325 2429 496 Cuts at: 6132 2040 Size: 2341 Fragments arranged by size: 2429 2341 496 389 385 325 33 21 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2848 3181 1163 Size: 573 1112 333 4415 Fragments arranged by size: 4415 1112 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2982 3883 4644 5672 1540 Size: 275 186 981 901 761 1028 2301 Fragments arranged by size: 2301 1028 981 901 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2186 2658 4494 4579 5650 2186 Size: 472 1836 85 1071 2969 Fragments arranged by size: 2969 1836 1071 472 85 BsmI GAATG_Cn' Cuts at: 3944 4037 3944 Size: 93 6340 BsmBI CGTCTCn'nnnn_ Cuts at: 2198 2198 Size: 6433 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2091 2186 2309 2366 2430 2658 Size: 399 224 77 95 123 57 64 228 Cuts at: 2658 3186 3314 3658 3752 4494 4579 5650 1391 Size: 528 128 344 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 528 399 344 228 224 128 123 95 94 85 77 64 57 BspEI T'CCGG_A Cuts at: 6096 6096 Size: 6433 BspHI T'CATG_A Cuts at: 4322 6052 4322 Size: 1730 4703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2038 2195 2447 2534 2870 3788 844 Size: 1194 157 252 87 336 918 3489 Fragments arranged by size: 3489 1194 918 336 252 157 87 BsrBI CCG'CTC Cuts at: 1394 1823 3144 3583 4320 1394 Size: 429 1321 439 737 3507 Fragments arranged by size: 3507 1321 737 439 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2808 3274 4928 5102 65 Size: 1183 1560 466 1654 174 1396 Fragments arranged by size: 1654 1560 1396 1183 466 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2025 2132 2709 5077 5264 1700 Size: 110 215 107 577 2368 187 2869 Fragments arranged by size: 2869 2368 577 215 187 110 107 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6433 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6433 BssSI C'ACGA_G Cuts at: 4493 5505 4493 Size: 1012 5421 BstAPI GCAn_nnn'nTGC Cuts at: 794 3653 794 Size: 2859 3574 BstBI TT'CG_AA Cuts at: 5237 5237 Size: 6433 BstEII G'GTnAC_C Cuts at: 1184 2665 2800 1184 Size: 1481 135 4817 Fragments arranged by size: 4817 1481 135 BstXI CCAn_nnnn'nTGG Cuts at: 2786 2786 Size: 6433 BstYI r'GATC_y Cuts at: 1972 2853 3763 4130 4515 4532 5984 6070 6082 Size: 881 910 367 385 17 1452 86 12 Cuts at: 6082 6428 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 910 881 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2891 2891 Size: 6433 BtgI C'CryG_G Cuts at: 513 1065 2670 513 Size: 552 1605 4276 Fragments arranged by size: 4276 1605 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5694 Fragments arranged by size: 5694 683 56 BtsI GCAGTG_nn' Cuts at: 3082 3949 4743 4763 3082 Size: 867 794 20 4752 Fragments arranged by size: 4752 867 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6433 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2233 2268 529 Size: 35 1669 35 4694 Fragments arranged by size: 4694 1669 35 35 DraI TTT'AAA Cuts at: 3737 3737 Size: 6433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5440 809 Size: 507 4124 1802 Fragments arranged by size: 4124 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2386 2514 2979 Size: 54 1425 50 275 18 556 128 465 Cuts at: 2979 3079 3153 3615 3880 4769 6398 8 Size: 100 74 462 265 889 1629 43 Fragments arranged by size: 1629 1425 889 556 465 462 275 265 128 100 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2979 3880 1537 Size: 275 1167 901 4090 Fragments arranged by size: 4090 1167 901 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3015 4363 1353 Size: 1662 1348 3423 Fragments arranged by size: 3423 1662 1348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5020 5394 5540 387 Size: 1064 138 3431 374 146 1280 Fragments arranged by size: 3431 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3011 4496 5093 5880 6148 1239 Size: 393 1379 1485 597 787 268 1524 Fragments arranged by size: 1524 1485 1379 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3319 3503 3319 Size: 184 6249 EcoO109I rG'GnC_Cy Cuts at: 2011 2305 2887 3182 3183 3466 2011 Size: 294 582 295 1 283 4978 Fragments arranged by size: 4978 582 295 294 283 1 EcoRI G'AATT_C Cuts at: 3742 4120 3742 Size: 378 6055 FseI GG_CCGG'CC Cuts at: 2713 2713 Size: 6433 FspI TGC'GCA Cuts at: 4939 4939 Size: 6433 HaeII r_GCGC'y Cuts at: 1678 2060 2108 3337 5580 1678 Size: 382 48 1229 2243 2531 Fragments arranged by size: 2531 2243 1229 382 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5074 5106 5148 5180 1373 Size: 32 135 32 3502 32 42 32 2626 Fragments arranged by size: 3502 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3777 4023 669 Size: 988 281 1839 246 3079 Fragments arranged by size: 3079 1839 988 281 246 HindIII A'AGCT_T Cuts at: 748 3671 748 Size: 2923 3510 HpaI GTT'AAC Cuts at: 4023 4023 Size: 6433 KpnI G_GTAC'C Cuts at: 3758 3758 Size: 6433 MfeI C'AATT_G Cuts at: 4032 4032 Size: 6433 MluI A'CGCG_T Cuts at: 6309 6309 Size: 6433 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2724 5547 5731 1305 Size: 1419 2823 184 2007 Fragments arranged by size: 2823 2007 1419 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2388 3617 10 Size: 54 1425 343 556 1229 2826 Fragments arranged by size: 2826 1425 1229 556 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2288 2784 3042 3590 3617 4391 4750 Size: 648 1122 496 258 548 27 774 359 Cuts at: 4750 4909 518 Size: 159 2042 Fragments arranged by size: 2042 1122 774 648 548 496 359 258 159 27 MspA1I CmG'CkG Cuts at: 1606 2415 2472 2818 2839 2908 3033 3151 3849 Size: 809 57 346 21 69 125 118 698 Cuts at: 3849 4526 5674 5919 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 698 677 346 245 125 118 69 57 21 NaeI GCC'GGC Cuts at: 2134 2711 2134 Size: 577 5856 NcoI C'CATG_G Cuts at: 513 1065 2670 513 Size: 552 1605 4276 Fragments arranged by size: 4276 1605 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6433 NgoMIV G'CCGG_C Cuts at: 2132 2709 2132 Size: 577 5856 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6433 NotI GC'GGCC_GC Cuts at: 3880 3880 Size: 6433 NspI r_CATG'y Cuts at: 1132 1229 2320 2337 3130 3549 3791 5336 1132 Size: 97 1091 17 793 419 242 1545 2229 Fragments arranged by size: 2229 1545 1091 793 419 242 97 17 PasI CC'CwG_GG Cuts at: 1348 3321 1348 Size: 1973 4460 PciI A'CATG_T Cuts at: 2333 5332 2333 Size: 2999 3434 PflMI CCAn_nnn'nTGG Cuts at: 1108 2166 1108 Size: 1058 5375 PmeI GTTT'AAAC Cuts at: 3737 3737 Size: 6433 PmlI CAC'GTG Cuts at: 3535 3535 Size: 6433 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4502 4534 6015 6047 4502 Size: 32 1481 32 4888 Fragments arranged by size: 4888 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2887 3466 2887 Size: 579 5854 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6433 PsiI TTA'TAA Cuts at: 4003 4003 Size: 6433 PspOMI G'GGCC_C Cuts at: 2010 2305 3182 2010 Size: 295 877 5261 Fragments arranged by size: 5261 877 295 PstI C_TGCA'G Cuts at: 830 2192 2358 2844 2938 3180 3785 830 Size: 1362 166 486 94 242 605 3478 Fragments arranged by size: 3478 1362 605 486 242 166 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6433 PvuII CAG'CTG Cuts at: 2818 2839 2818 Size: 21 6412 SalI G'TCGA_C Cuts at: 1936 3775 1936 Size: 1839 4594 SapI GCTCTTCn'nnn_ Cuts at: 3015 3015 Size: 6433 SbfI CC_TGCA'GG Cuts at: 2192 3180 3785 2192 Size: 988 605 4840 Fragments arranged by size: 4840 988 605 ScaI AGT'ACT Cuts at: 4681 6393 4681 Size: 1712 4721 SfcI C'TryA_G Cuts at: 826 1045 2188 2354 2840 2934 3176 3781 4916 Size: 219 1143 166 486 94 242 605 1135 Cuts at: 4916 5597 5788 826 Size: 681 191 1471 Fragments arranged by size: 1471 1143 1135 681 605 486 242 219 191 166 94 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6433 SmaI CCC'GGG Cuts at: 1872 3760 1872 Size: 1888 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3491 3710 4537 5438 5700 820 Size: 197 926 1548 219 827 901 262 1553 Fragments arranged by size: 1553 1548 926 901 827 262 219 197 SnaBI TAC'GTA Cuts at: 493 4224 493 Size: 3731 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6433 SphI G_CATG'C Cuts at: 1132 2320 3130 3549 3791 1132 Size: 1188 810 419 242 3774 Fragments arranged by size: 3774 1188 810 419 242 SspI AAT'ATT Cuts at: 5 52 4357 5 Size: 47 4305 2081 Fragments arranged by size: 4305 2081 47 StuI AGG'CCT Cuts at: 1517 2492 3122 3296 1517 Size: 975 630 174 4654 Fragments arranged by size: 4654 975 630 174 StyI C'CwwG_G Cuts at: 513 1065 2015 2322 2670 3872 513 Size: 552 950 307 348 1202 3074 Fragments arranged by size: 3074 1202 950 552 348 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 2653 2663 3437 4460 4477 4630 Size: 1255 578 495 10 774 1023 17 153 Cuts at: 4630 4815 5260 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1023 774 578 495 445 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4679 6391 96 Size: 274 80 33 51 1443 2702 1712 138 Fragments arranged by size: 2702 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2201 2233 3661 3693 6393 6425 2201 Size: 32 1428 32 2700 32 2209 Fragments arranged by size: 2700 2209 1428 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3392 1316 Size: 2076 4357 XbaI T'CTAG_A Cuts at: 3769 3769 Size: 6433 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2266 2167 Size: 99 6334 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6433 XmaI C'CCGG_G Cuts at: 1870 3758 1870 Size: 1888 4545 XmnI GAAnn'nnTTC Cuts at: 4562 4562 Size: 6433 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3706 4241 276 Size: 53 83 186 936 2172 535 2468 Fragments arranged by size: 2468 2172 936 535 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI