GMCL1 ORF clone pFN21AB5961

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5961

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Clone info.

Species

human

Clone Name

pFN21AB5961 Sequence (with vector) Restriction Map

Product ID

FHC03909

Flexi Clone PID

FXC03909

ORF Sequence

DNA sequence (1545 bp)
Protein sequence (515 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527982]

Gene

KIBB5961

Symbol

GMCL1

Alias : BTBD13, GCL, GCL1, SPATA29

Description

germ cell-less, spermatogenesis associated 1

Original Clone ID

cs00661

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1545 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 515 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1895.1 1e-218 100.0 GMCL1 CCDS3245.2 8.9e-12 25.8 KLHL6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 100 206 PF00651 BTB/POZ IPR011705 214 283 PF07707 BTB/Kelch-associated HMMSmart IPR000210 108 208 SM00225 BTB/POZ-like IPR011705 213 300 SM00875 BTB/Kelch-associated ProfileScan IPR000210 108 178 PS50097 BTB/POZ-like Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5961 Download

>KIBB5961  1545 bp
ATGGGATCGTTGAGCAGCCGGGTGCTGCGCCAGCCAAGACCAGCCCTTGCCCAGCAGGCG
CAGGGTGCCAGGGCGGGGGGCTCGGCCCGGAGGCCGGACACTGGAGACGATGCGGCGGGC
CACGGATTCTGTTACTGTGCGGGCAGCCACAAGCGCAAGCGGAGCAGCGGGTCCTTCTGC
TACTGTCACCCTGACTCGGAGACGGACGAGGATGAGGAGGAGGGGGACGAGCAGCAGCGG
CTCCTCAACACCCCTCGAAGGAAAAAATTAAAGAGTACATCTAAATATATTTATCAAACA
TTATTTTTGAATGGTGAAAACAGTGACATTAAGATTTGTGCTCTAGGAGAAGAATGGAGC
TTACACAAAATATATTTATGTCAATCTGGCTACTTTTCTAGTATGTTCAGTGGTTCTTGG
AAAGAATCCAGCATGAATATTATTGAACTGGAGATTCCTGACCAGAACATTGATGTAGAA
GCACTGCAGGTTGCATTTGGTTCACTGTATCGAGATGATGTCTTGATAAAGCCCAGTCGA
GTTGTTGCCATTTTGGCAGCAGCTTGTTTGCTGCAGTTGGACGGTTTAATACAGCAGTGT
GGTGAGACAATGAAGGAAACAGTTAATGTGAAAACTGTATGTGGCTATTACACATCAGCA
GGGACCTATGGATTAGATTCTGTAAAGAAAAAGTGCCTTGAATGGCTTCTAAACAATTTG
ATGACTCACCAGAATGTTGAACTTTTTAAAGAACTCAGTATAAATGTCATGAAACAGCTC
ATTGGTTCATCTAACTTATTTGTGATGCAAGTGGAGATGGATATATACACTGCTCTAAAA
AAGTGGATGTTCCTTCAACTTGTGCCTTCTTGGAATGGATCTTTAAAACAGCTTTTGACA
GAAACAGATGTCTGGTTTTCTAAACAGAGGAAAGATTTTGAAGGTATGGCCTTTCTTGAA
ACTGAACAAGGAAAACCATTTGTGTCAGTATTCAGACATTTAAGGTTACAATATATTATC
AGTGATCTGGCTTCTGCAAGAATTATTGAACAAGATGCTGTAGTACCTTCAGAATGGCTC
TCTTCTGTGTATAAACAGCAGTGGTTTGCTATGCTGCGGGCAGAACAGGACAGTGAGGTG
GGGCCTCAAGAAATCAATAAAGAAGAACTAGAGGGAAACAGCATGAGGTGTGGTAGAAAG
CTTGCCAAAGATGGTGAATACTGCTGGCGTTGGACAGGTTTTAACTTCGGCTTCGACCTA
CTTGTAACTTACACCAATCGATACATCATTTTCAAACGCAATACACTGAATCAGCCATGT
AGCGGATCTGTCAGTTTACAGCCTCGAAGGAGCATAGCATTTAGATTACGTTTGGCTTCT
TTTGATAGTAGTGGAAAACTAATATGTAGTAGAACAACTGGCTATCAAATACTTACACTT
GAAAAGGATCAGGAACAAGTGGTGATGAACTTGGACAGCAGGCTTCTGATCTTCCCTTTA
TATATCTGCTGTAACTTCTTGTATATATCACCAGAAAAAAAGAAT

ORF Protein

Cloned ORF protein sequence for pFN21AB5961 Download

>KIBB5961  515 aa
MGSLSSRVLRQPRPALAQQAQGARAGGSARRPDTGDDAAGHGFCYCAGSHKRKRSSGSFC
YCHPDSETDEDEEEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLNGENSDIKICALGEEWS
LHKIYLCQSGYFSSMFSGSWKESSMNIIELEIPDQNIDVEALQVAFGSLYRDDVLIKPSR
VVAILAAACLLQLDGLIQQCGETMKETVNVKTVCGYYTSAGTYGLDSVKKKCLEWLLNNL
MTHQNVELFKELSINVMKQLIGSSNLFVMQVEMDIYTALKKWMFLQLVPSWNGSLKQLLT
ETDVWFSKQRKDFEGMAFLETEQGKPFVSVFRHLRLQYIISDLASARIIEQDAVVPSEWL
SSVYKQQWFAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDL
LVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRTTGYQILTL
EKDQEQVVMNLDSRLLIFPLYICCNFLYISPEKKN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5961 Download

>pFN21AB5961  6250 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGATCGTTGAGCAGCCGGGTGCTGCGCCAGCC
AAGACCAGCCCTTGCCCAGCAGGCGCAGGGTGCCAGGGCGGGGGGCTCGGCCCGGAGGCC
GGACACTGGAGACGATGCGGCGGGCCACGGATTCTGTTACTGTGCGGGCAGCCACAAGCG
CAAGCGGAGCAGCGGGTCCTTCTGCTACTGTCACCCTGACTCGGAGACGGACGAGGATGA
GGAGGAGGGGGACGAGCAGCAGCGGCTCCTCAACACCCCTCGAAGGAAAAAATTAAAGAG
TACATCTAAATATATTTATCAAACATTATTTTTGAATGGTGAAAACAGTGACATTAAGAT
TTGTGCTCTAGGAGAAGAATGGAGCTTACACAAAATATATTTATGTCAATCTGGCTACTT
TTCTAGTATGTTCAGTGGTTCTTGGAAAGAATCCAGCATGAATATTATTGAACTGGAGAT
TCCTGACCAGAACATTGATGTAGAAGCACTGCAGGTTGCATTTGGTTCACTGTATCGAGA
TGATGTCTTGATAAAGCCCAGTCGAGTTGTTGCCATTTTGGCAGCAGCTTGTTTGCTGCA
GTTGGACGGTTTAATACAGCAGTGTGGTGAGACAATGAAGGAAACAGTTAATGTGAAAAC
TGTATGTGGCTATTACACATCAGCAGGGACCTATGGATTAGATTCTGTAAAGAAAAAGTG
CCTTGAATGGCTTCTAAACAATTTGATGACTCACCAGAATGTTGAACTTTTTAAAGAACT
CAGTATAAATGTCATGAAACAGCTCATTGGTTCATCTAACTTATTTGTGATGCAAGTGGA
GATGGATATATACACTGCTCTAAAAAAGTGGATGTTCCTTCAACTTGTGCCTTCTTGGAA
TGGATCTTTAAAACAGCTTTTGACAGAAACAGATGTCTGGTTTTCTAAACAGAGGAAAGA
TTTTGAAGGTATGGCCTTTCTTGAAACTGAACAAGGAAAACCATTTGTGTCAGTATTCAG
ACATTTAAGGTTACAATATATTATCAGTGATCTGGCTTCTGCAAGAATTATTGAACAAGA
TGCTGTAGTACCTTCAGAATGGCTCTCTTCTGTGTATAAACAGCAGTGGTTTGCTATGCT
GCGGGCAGAACAGGACAGTGAGGTGGGGCCTCAAGAAATCAATAAAGAAGAACTAGAGGG
AAACAGCATGAGGTGTGGTAGAAAGCTTGCCAAAGATGGTGAATACTGCTGGCGTTGGAC
AGGTTTTAACTTCGGCTTCGACCTACTTGTAACTTACACCAATCGATACATCATTTTCAA
ACGCAATACACTGAATCAGCCATGTAGCGGATCTGTCAGTTTACAGCCTCGAAGGAGCAT
AGCATTTAGATTACGTTTGGCTTCTTTTGATAGTAGTGGAAAACTAATATGTAGTAGAAC
AACTGGCTATCAAATACTTACACTTGAAAAGGATCAGGAACAAGTGGTGATGAACTTGGA
CAGCAGGCTTCTGATCTTCCCTTTATATATCTGCTGTAACTTCTTGTATATATCACCAGA
AAAAAAGAATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT