(Circular) (Six-base) MAPSORT of: pFN21AB5961.seq Check: 1132 from: 1 to: 6250 pFN21AB5961 6250 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4060 278 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3593 1937 Size: 1656 4594 Acc65I G'GTAC_C Cuts at: 3571 3571 Size: 6250 AclI AA'CG_TT Cuts at: 1563 4377 4750 1563 Size: 2814 373 3063 Fragments arranged by size: 3063 2814 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3057 4313 5697 5965 1632 Size: 1425 1256 1384 268 1917 Fragments arranged by size: 1917 1425 1384 1256 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6053 AflIII A'CryG_T Cuts at: 1203 5149 6126 1203 Size: 3946 977 1327 Fragments arranged by size: 3946 1327 977 AgeI A'CCGG_T Cuts at: 5081 5081 Size: 6250 AhdI GACnn_n'nnGTC Cuts at: 4979 4979 Size: 6250 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6250 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2042 3126 1184 Size: 858 1084 4308 Fragments arranged by size: 4308 1084 858 AlwNI CAG_nnn'CTG Cuts at: 3993 5565 3993 Size: 1572 4678 ApaLI G'TGCA_C Cuts at: 4307 5463 4307 Size: 1156 5094 ApoI r'AATT_y Cuts at: 1493 3559 3785 3937 6046 1493 Size: 2066 226 152 2109 1697 Fragments arranged by size: 2109 2066 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4804 160 Size: 872 3772 1606 Fragments arranged by size: 3772 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 3575 1870 Size: 73 1632 4545 Fragments arranged by size: 4545 1632 73 BamHI G'GATC_C Cuts at: 3580 3947 3580 Size: 367 5883 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 3571 5026 618 Size: 325 217 627 282 1502 1455 1842 Fragments arranged by size: 1842 1502 1455 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 2087 3569 1391 Size: 696 1482 4072 Fragments arranged by size: 4072 1482 696 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5279 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4441 4475 1903 Size: 34 2504 34 3678 Fragments arranged by size: 3678 2504 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4142 5352 4142 Size: 1210 5040 BclI T'GATC_A Cuts at: 1548 1548 Size: 6250 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 2558 4861 Size: 107 122 71 631 605 388 498 2303 Cuts at: 4861 136 Size: 1525 Fragments arranged by size: 2303 1525 631 605 498 388 122 107 71 BglII A'GATC_T Cuts at: 6245 6245 Size: 6250 BlpI GC'TnA_GC Cuts at: 3667 3667 Size: 6250 Bme1580I G_kGCm'C Cuts at: 1790 4311 5467 1790 Size: 2521 1156 2573 Fragments arranged by size: 2573 2521 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2532 3981 4939 Size: 416 53 83 399 24 1115 1449 958 Cuts at: 4939 442 Size: 1753 Fragments arranged by size: 1753 1449 1115 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 2474 4910 1239 Size: 888 347 2436 2579 Fragments arranged by size: 2579 2436 888 347 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3135 4375 5240 5538 3135 Size: 1240 865 298 3847 Fragments arranged by size: 3847 1240 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4913 882 Size: 617 3414 2219 Fragments arranged by size: 3414 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4041 493 Size: 711 2837 2702 Fragments arranged by size: 2837 2702 711 BsaBI GATnn'nnATC Cuts at: 6244 6244 Size: 6250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4057 4439 5074 275 Size: 53 83 186 936 2524 382 635 1451 Fragments arranged by size: 2524 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4683 5081 5355 5502 5913 4683 Size: 398 274 147 411 5020 Fragments arranged by size: 5020 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6025 Fragments arranged by size: 6025 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 2237 1186 Size: 1048 3 5199 Fragments arranged by size: 5199 1048 3 BseYI C'CCAG_C Cuts at: 2055 5453 5949 2055 Size: 3398 496 2356 Fragments arranged by size: 3398 2356 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5677 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3700 4461 5489 1540 Size: 275 186 1699 761 1028 2301 Fragments arranged by size: 2301 1699 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2347 4311 4396 5467 2347 Size: 1964 85 1071 3130 Fragments arranged by size: 3130 1964 1071 85 BsmI GAATG_Cn' Cuts at: 3761 3854 3761 Size: 93 6157 BsmBI CGTCTCn'nnnn_ Cuts at: 2103 2198 2103 Size: 95 6155 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2347 3569 4311 4396 5467 1391 Size: 399 297 260 1222 742 85 1071 2174 Fragments arranged by size: 2174 1222 1071 742 399 297 260 85 BspEI T'CCGG_A Cuts at: 5913 5913 Size: 6250 BspHI T'CATG_A Cuts at: 2772 4139 5869 2772 Size: 1367 1730 3153 Fragments arranged by size: 3153 1730 1367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2482 3605 844 Size: 1638 1123 3489 Fragments arranged by size: 3489 1638 1123 BsrBI CCG'CTC Cuts at: 1394 1823 4137 1394 Size: 429 2314 3507 Fragments arranged by size: 3507 2314 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4745 4919 65 Size: 1183 3497 174 1396 Fragments arranged by size: 3497 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4894 5081 1700 Size: 110 3084 187 2869 Fragments arranged by size: 3084 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6250 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6250 BssSI C'ACGA_G Cuts at: 4310 5322 4310 Size: 1012 5238 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6250 BstBI TT'CG_AA Cuts at: 5054 5054 Size: 6250 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6250 BstYI r'GATC_y Cuts at: 1972 2882 3329 3580 3947 4332 4349 5801 5887 Size: 910 447 251 367 385 17 1452 86 Cuts at: 5887 5899 6245 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 910 447 385 367 346 251 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2125 513 Size: 552 939 121 4638 Fragments arranged by size: 4638 939 552 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5511 Fragments arranged by size: 5511 683 56 BtsI GCAGTG_nn' Cuts at: 2486 2606 2832 3110 3766 4560 4580 2486 Size: 120 226 278 656 794 20 4156 Fragments arranged by size: 4156 794 656 278 226 120 20 ClaI AT'CG_AT Cuts at: 1554 3283 1554 Size: 1729 4521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6215 DraI TTT'AAA Cuts at: 2752 2889 3554 2752 Size: 137 665 5448 Fragments arranged by size: 5448 665 137 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5257 809 Size: 507 3941 1802 Fragments arranged by size: 3941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3697 4586 6215 Size: 54 1425 50 275 18 1867 889 1629 Cuts at: 6215 8 Size: 43 Fragments arranged by size: 1867 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3697 1537 Size: 275 1885 4090 Fragments arranged by size: 4090 1885 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3091 4180 1353 Size: 1738 1089 3423 Fragments arranged by size: 3423 1738 1089 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4837 5211 5357 387 Size: 1064 138 3248 374 146 1280 Fragments arranged by size: 3248 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2474 3057 4313 4910 5697 5965 Size: 393 495 347 583 1256 597 787 268 Cuts at: 5965 1239 Size: 1524 Fragments arranged by size: 1524 1256 787 597 583 495 393 347 268 EcoO109I rG'GnC_Cy Cuts at: 2175 2667 3146 2175 Size: 492 479 5279 Fragments arranged by size: 5279 492 479 EcoRI G'AATT_C Cuts at: 3559 3937 3559 Size: 378 5872 FspI TGC'GCA Cuts at: 4756 4756 Size: 6250 HaeII r_GCGC'y Cuts at: 1678 5397 1678 Size: 3719 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2329 2361 2508 2540 4891 Size: 32 135 32 757 32 147 32 2351 Cuts at: 4891 4923 4965 4997 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2351 757 147 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3594 3840 669 Size: 988 281 1656 246 3079 Fragments arranged by size: 3079 1656 988 281 246 HindIII A'AGCT_T Cuts at: 748 3203 748 Size: 2455 3795 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KpnI G_GTAC'C Cuts at: 3575 3575 Size: 6250 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 6126 6126 Size: 6250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2562 3215 5364 5548 1305 Size: 1257 653 2149 184 2007 Fragments arranged by size: 2149 2007 1257 653 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4428 Fragments arranged by size: 4428 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2736 4208 4567 4726 518 Size: 648 1570 1472 359 159 2042 Fragments arranged by size: 2042 1570 1472 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2172 2242 3666 4343 5491 5736 1606 Size: 566 70 1424 677 1148 245 2120 Fragments arranged by size: 2120 1424 1148 677 566 245 70 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4759 Fragments arranged by size: 4759 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6250 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6250 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NspI r_CATG'y Cuts at: 1132 1229 3608 5153 1132 Size: 97 2379 1545 2229 Fragments arranged by size: 2379 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6250 PciI A'CATG_T Cuts at: 5149 5149 Size: 6250 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6250 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4319 4351 5832 5864 4319 Size: 32 1481 32 4705 Fragments arranged by size: 4705 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2175 2667 2175 Size: 492 5758 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 3820 3820 Size: 6250 PstI C_TGCA'G Cuts at: 830 2493 2580 3602 830 Size: 1663 87 1022 3478 Fragments arranged by size: 3478 1663 1022 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 4498 6210 4498 Size: 1712 4538 SfcI C'TryA_G Cuts at: 826 1045 2489 2576 3063 3598 4733 5414 5605 Size: 219 1444 87 487 535 1135 681 191 Cuts at: 5605 826 Size: 1471 Fragments arranged by size: 1471 1444 1135 681 535 487 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 3577 1872 Size: 1705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3150 4354 5255 5517 820 Size: 197 926 1207 1204 901 262 1553 Fragments arranged by size: 1553 1207 1204 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6250 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SspI AAT'ATT Cuts at: 5 52 2443 4174 5 Size: 47 2391 1731 2081 Fragments arranged by size: 2391 2081 1731 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6250 StyI C'CwwG_G Cuts at: 513 1065 2004 3689 513 Size: 552 939 1685 3074 Fragments arranged by size: 3074 1685 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4277 4294 4447 4632 5077 325 Size: 1255 2697 17 153 185 445 1498 Fragments arranged by size: 2697 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2279 4496 6208 Size: 274 80 33 51 1443 302 2217 1712 Cuts at: 6208 96 Size: 138 Fragments arranged by size: 2217 1712 1443 302 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6210 6242 6210 Size: 32 6218 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6250 XbaI T'CTAG_A Cuts at: 3586 3586 Size: 6250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 3575 1870 Size: 1705 4545 XmnI GAAnn'nnTTC Cuts at: 2709 4379 2709 Size: 1670 4580 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4058 276 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI