HSPA14 ORF clone pFN21AB5949

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5949

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Clone info.

Species

human

Clone Name

pFN21AB5949 Sequence (with vector) Restriction Map

Product ID

FHC11954

Flexi Clone PID

FXC11954

ORF Sequence

DNA sequence (1527 bp)
Protein sequence (509 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587406]

Gene

KIBB5949

Symbol

HSPA14

Alias : HSP70-4, HSP70L1

Description

heat shock 70kDa protein 14, transcript variant 1

Original Clone ID

cs00649

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1527 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 509 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7103.1 0 100.0 HSPA14 CCDS9766.1 1.2e-70 35.6 HSPA2 CCDS34415.1 4.3e-70 35.2 HSPA1B CCDS34414.1 4.3e-70 35.2 HSPA1A CCDS1231.1 6e-70 35.7 HSPA6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001023 2 15 PR00301 Heat shock protein Hsp70 IPR001023 30 42 PR00301 Heat shock protein Hsp70 IPR001023 139 159 PR00301 Heat shock protein Hsp70 IPR001023 329 345 PR00301 Heat shock protein Hsp70 IPR001023 361 381 PR00301 Heat shock protein Hsp70 HMMPfam IPR013126 3 506 PF00012 Heat shock protein 70
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057383 509 610369 100.0 100.0 NP_068814 639 140560 35.6 98.8 NP_005336 641 140550 35.2 99.0 NP_005337 641 603012 35.2 99.0 NP_002146 643 140555 35.7 98.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5949 Download

>KIBB5949  1527 bp
ATGGCGGCCATCGGAGTTCACCTGGGCTGCACCTCAGCCTGTGTGGCCGTCTATAAGGAT
GGCCGGGCTGGTGTGGTTGCAAATGATGCCGGTGACCGAGTTACTCCAGCTGTTGTTGCT
TACTCAGAAAATGAAGAGATTGTTGGATTGGCAGCAAAACAAAGTAGAATAAGAAATATT
TCAAATACAGTAATGAAAGTAAAGCAGATCCTGGGCAGAAGCTCCAGTGATCCACAAGCT
CAGAAATACATCGCGGAAAGTAAATGTTTAGTCATTGAAAAAAATGGGAAATTACGATAT
GAAATAGATACTGGAGAAGAAACAAAATTTGTTAACCCAGAAGATGTTGCCAGACTGATA
TTTAGTAAAATGAAAGAAACGGCACATTCTGTATTGGGCTCAGATGCAAATGATGTAGTT
ATTACTGTCCCGTTTGATTTTGGAGAAAAGCAAAAAAATGCTCTTGGAGAAGCAGCTAGA
GCTGCTGGATTTAATGTTTTGCGATTAATTCACGAACCGTCTGCAGCTCTTCTTGCTTAT
GGAATTGGACAAGACTCCCCTACTGGAAAAAGCAATATTTTGGTGTTTAAGCTTGGAGGA
ACATCCTTATCTCTCAGCGTCATGGAAGTTAACAGTGGAATATATCGGGTTCTTTCAACA
AACACTGATGATAACATCGGTGGTGCACATTTCACAGAAACCTTAGCACAGTATCTAGCT
TCTGAGTTCCAAAGATCCTTCAAACATGATGTGAGAGGAAATGCGCGAGCCATGATGAAA
TTAACGAACAGTGCTGAAGTAGCGAAACATTCTTTGTCAACCTTGGGAAGTGCCAACTGT
TTTCTTGACTCATTATATGAAGGTCAAGATTTTGATTGCAATGTGTCCAGAGCAAGATTT
GAACTTCTTTGTTCTCCACTTTTTAATAAGTGTATAGAAGCAATCAGAGGACTCTTAGAT
CAAAATGGATTTACAGCAGATGATATCAACAAGGTTGTCCTTTGTGGAGGGTCTTCTCGA
ATCCCAAAGCTACAGCAACTGATTAAAGATCTTTTCCCAGCTGTTGAGCTTCTCAATTCT
ATCCCTCCTGATGAAGTGATCCCTATTGGTGCAGCTATAGAAGCAGGAATTCTTATTGGG
AAAGAAAACCTGTTGGTGGAAGACTCTCTTATGATAGAGTGTTCAGCCAGAGATATTTTA
GTTAAGGGTGTGGACGAATCAGGAGCCAGTAGATTCACAGTGCTGTTTCCATCAGGGACT
CCTTTGCCAGCTCGAAGACAACACACATTGCAAGCCCCTGGAAGCATATCTTCAGTGTGC
CTTGAACTCTATGAGTCTGATGGGAAGAACTCTGCCAAAGAGGAAACCAAGTTTGCACAG
GTTGTACTCCAGGATTTAGATAAAAAAGAAAATGGATTACGTGATATATTAGCTGTTCTT
ACTATGAAAAGGGATGGATCTTTACATGTGACATGCACAGATCAAGAAACTGGAAAATGT
GAAGCAATCTCTATTGAGATAGCATCT

ORF Protein

Cloned ORF protein sequence for pFN21AB5949 Download

>KIBB5949  509 aa
MAAIGVHLGCTSACVAVYKDGRAGVVANDAGDRVTPAVVAYSENEEIVGLAAKQSRIRNI
SNTVMKVKQILGRSSSDPQAQKYIAESKCLVIEKNGKLRYEIDTGEETKFVNPEDVARLI
FSKMKETAHSVLGSDANDVVITVPFDFGEKQKNALGEAARAAGFNVLRLIHEPSAALLAY
GIGQDSPTGKSNILVFKLGGTSLSLSVMEVNSGIYRVLSTNTDDNIGGAHFTETLAQYLA
SEFQRSFKHDVRGNARAMMKLTNSAEVAKHSLSTLGSANCFLDSLYEGQDFDCNVSRARF
ELLCSPLFNKCIEAIRGLLDQNGFTADDINKVVLCGGSSRIPKLQQLIKDLFPAVELLNS
IPPDEVIPIGAAIEAGILIGKENLLVEDSLMIECSARDILVKGVDESGASRFTVLFPSGT
PLPARRQHTLQAPGSISSVCLELYESDGKNSAKEETKFAQVVLQDLDKKENGLRDILAVL
TMKRDGSLHVTCTDQETGKCEAISIEIAS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5949 Download

>pFN21AB5949  6232 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCCATCGGAGTTCACCTGGGCTGCACCTC
AGCCTGTGTGGCCGTCTATAAGGATGGCCGGGCTGGTGTGGTTGCAAATGATGCCGGTGA
CCGAGTTACTCCAGCTGTTGTTGCTTACTCAGAAAATGAAGAGATTGTTGGATTGGCAGC
AAAACAAAGTAGAATAAGAAATATTTCAAATACAGTAATGAAAGTAAAGCAGATCCTGGG
CAGAAGCTCCAGTGATCCACAAGCTCAGAAATACATCGCGGAAAGTAAATGTTTAGTCAT
TGAAAAAAATGGGAAATTACGATATGAAATAGATACTGGAGAAGAAACAAAATTTGTTAA
CCCAGAAGATGTTGCCAGACTGATATTTAGTAAAATGAAAGAAACGGCACATTCTGTATT
GGGCTCAGATGCAAATGATGTAGTTATTACTGTCCCGTTTGATTTTGGAGAAAAGCAAAA
AAATGCTCTTGGAGAAGCAGCTAGAGCTGCTGGATTTAATGTTTTGCGATTAATTCACGA
ACCGTCTGCAGCTCTTCTTGCTTATGGAATTGGACAAGACTCCCCTACTGGAAAAAGCAA
TATTTTGGTGTTTAAGCTTGGAGGAACATCCTTATCTCTCAGCGTCATGGAAGTTAACAG
TGGAATATATCGGGTTCTTTCAACAAACACTGATGATAACATCGGTGGTGCACATTTCAC
AGAAACCTTAGCACAGTATCTAGCTTCTGAGTTCCAAAGATCCTTCAAACATGATGTGAG
AGGAAATGCGCGAGCCATGATGAAATTAACGAACAGTGCTGAAGTAGCGAAACATTCTTT
GTCAACCTTGGGAAGTGCCAACTGTTTTCTTGACTCATTATATGAAGGTCAAGATTTTGA
TTGCAATGTGTCCAGAGCAAGATTTGAACTTCTTTGTTCTCCACTTTTTAATAAGTGTAT
AGAAGCAATCAGAGGACTCTTAGATCAAAATGGATTTACAGCAGATGATATCAACAAGGT
TGTCCTTTGTGGAGGGTCTTCTCGAATCCCAAAGCTACAGCAACTGATTAAAGATCTTTT
CCCAGCTGTTGAGCTTCTCAATTCTATCCCTCCTGATGAAGTGATCCCTATTGGTGCAGC
TATAGAAGCAGGAATTCTTATTGGGAAAGAAAACCTGTTGGTGGAAGACTCTCTTATGAT
AGAGTGTTCAGCCAGAGATATTTTAGTTAAGGGTGTGGACGAATCAGGAGCCAGTAGATT
CACAGTGCTGTTTCCATCAGGGACTCCTTTGCCAGCTCGAAGACAACACACATTGCAAGC
CCCTGGAAGCATATCTTCAGTGTGCCTTGAACTCTATGAGTCTGATGGGAAGAACTCTGC
CAAAGAGGAAACCAAGTTTGCACAGGTTGTACTCCAGGATTTAGATAAAAAAGAAAATGG
ATTACGTGATATATTAGCTGTTCTTACTATGAAAAGGGATGGATCTTTACATGTGACATG
CACAGATCAAGAAACTGGAAAATGTGAAGCAATCTCTATTGAGATAGCATCTGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT