(Circular) (Six-base) MAPSORT of: pFN21AB5949.seq Check: 424 from: 1 to: 6232 pFN21AB5949 6232 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3575 1937 Size: 1638 4594 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2820 3300 4295 5679 5947 1632 Size: 1188 480 995 1384 268 1917 Fragments arranged by size: 1917 1384 1188 995 480 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 3469 5131 6108 1203 Size: 2266 1662 977 1327 Fragments arranged by size: 2266 1662 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2478 1184 Size: 1294 4938 AlwNI CAG_nnn'CTG Cuts at: 2215 3043 3975 5547 2215 Size: 828 932 1572 2900 Fragments arranged by size: 2900 1572 932 828 ApaLI G'TGCA_C Cuts at: 2688 4289 5445 2688 Size: 1601 1156 3475 Fragments arranged by size: 3475 1601 1156 ApoI r'AATT_y Cuts at: 1493 2330 3132 3541 3767 3919 6028 1493 Size: 837 802 409 226 152 2109 1697 Fragments arranged by size: 2109 1697 837 802 409 226 152 AseI AT'TA_AT Cuts at: 160 1032 2510 4786 160 Size: 872 1478 2276 1606 Fragments arranged by size: 2276 1606 1478 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 3557 1870 Size: 73 1614 4545 Fragments arranged by size: 4545 1614 73 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3553 5008 618 Size: 325 217 627 1766 1455 1842 Fragments arranged by size: 1842 1766 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2405 3551 1391 Size: 1014 1146 4072 Fragments arranged by size: 4072 1146 1014 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3009 3171 3286 928 Size: 971 1110 162 115 3874 Fragments arranged by size: 3874 1110 971 162 115 BbvCI CC'TCA_GC Cuts at: 2038 2038 Size: 6232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2507 2541 4423 4457 1903 Size: 34 570 34 1882 34 3678 Fragments arranged by size: 3678 1882 570 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 5334 4124 Size: 1210 5022 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2048 4843 Size: 107 122 71 631 605 337 39 2795 Cuts at: 4843 136 Size: 1525 Fragments arranged by size: 2795 1525 631 605 337 122 107 71 39 BglII A'GATC_T Cuts at: 3052 6227 3052 Size: 3175 3057 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 2692 4293 5449 1790 Size: 902 1601 1156 2573 Fragments arranged by size: 2573 1601 1156 902 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3963 4921 442 Size: 416 53 83 399 24 2546 958 1753 Fragments arranged by size: 2546 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2212 2337 3377 4892 1239 Size: 855 118 125 1040 1515 2579 Fragments arranged by size: 2579 1515 1040 855 125 118 Bpu10I CC'TnA_GC Cuts at: 2038 2707 2038 Size: 669 5563 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 5222 5520 4357 Size: 865 298 5069 Fragments arranged by size: 5069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4895 882 Size: 617 3396 2219 Fragments arranged by size: 3396 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3425 4023 493 Size: 711 2221 598 2702 Fragments arranged by size: 2702 2221 711 598 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6007 Fragments arranged by size: 6007 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6232 BseYI C'CCAG_C Cuts at: 3061 5435 5931 3061 Size: 2374 496 3362 Fragments arranged by size: 3362 2374 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2017 3135 1163 Size: 573 281 1118 4260 Fragments arranged by size: 4260 1118 573 281 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2692 4293 4378 5449 2692 Size: 1601 85 1071 3475 Fragments arranged by size: 3475 1601 1071 85 BsmI GAATG_Cn' Cuts at: 3743 3836 3743 Size: 93 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2405 2692 3551 4293 4378 5449 1391 Size: 399 615 287 859 742 85 1071 2174 Fragments arranged by size: 2174 1071 859 742 615 399 287 85 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 4121 5851 4121 Size: 1730 4502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3587 844 Size: 2743 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2890 3290 4727 4901 65 Size: 1183 1642 400 1437 174 1396 Fragments arranged by size: 1642 1437 1396 1183 400 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 4876 5063 1700 Size: 110 283 2783 187 2869 Fragments arranged by size: 2869 2783 283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 2090 794 Size: 1296 4936 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 2096 1184 Size: 912 5320 BstYI r'GATC_y Cuts at: 1972 2211 2738 3052 3461 3562 3929 4314 4331 Size: 239 527 314 409 101 367 385 17 Cuts at: 4331 5783 5869 5881 6227 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 527 409 385 367 346 314 239 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4741 Fragments arranged by size: 4741 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2239 487 Size: 683 56 1013 4480 Fragments arranged by size: 4480 1013 683 56 BtsI GCAGTG_nn' Cuts at: 3748 4542 4562 3748 Size: 794 20 5418 Fragments arranged by size: 5418 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2049 2065 Size: 54 1425 50 275 18 180 39 16 Cuts at: 2065 3679 4568 6197 8 Size: 1614 889 1629 43 Fragments arranged by size: 1629 1614 1425 889 275 180 54 50 43 39 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2538 4162 1353 Size: 780 405 1624 3423 Fragments arranged by size: 3423 1624 780 405 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2212 2337 2820 3300 3377 4295 Size: 393 462 118 125 483 480 77 918 Cuts at: 4295 4892 5679 5947 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 918 787 597 483 480 462 393 268 125 118 77 EcoRI G'AATT_C Cuts at: 3132 3541 3919 3132 Size: 409 378 5445 Fragments arranged by size: 5445 409 378 EcoRV GAT'ATC Cuts at: 2989 2989 Size: 6232 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3188 3330 3188 Size: 142 6090 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 5379 1678 Size: 3701 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3037 3069 3512 3544 4873 Size: 32 135 32 1465 32 443 32 1329 Cuts at: 4873 4905 4947 4979 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1465 1329 443 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2338 2635 2823 3576 3822 669 Size: 988 281 400 297 188 753 246 3079 Fragments arranged by size: 3079 988 753 400 297 281 246 188 HindIII A'AGCT_T Cuts at: 748 2594 748 Size: 1846 4386 HpaI GTT'AAC Cuts at: 2338 2635 3822 2338 Size: 297 1187 4748 Fragments arranged by size: 4748 1187 297 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2128 5346 5530 1305 Size: 823 3218 184 2007 Fragments arranged by size: 3218 2007 823 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4410 Fragments arranged by size: 4410 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4190 4549 4708 518 Size: 648 3024 359 159 2042 Fragments arranged by size: 3024 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 3065 3648 4325 5473 5718 1606 Size: 508 951 583 677 1148 245 2120 Fragments arranged by size: 2120 1148 951 677 583 508 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4741 Fragments arranged by size: 4741 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 3473 3480 3590 5135 1132 Size: 97 2244 7 110 1545 2229 Fragments arranged by size: 2244 2229 1545 110 97 7 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6232 PciI A'CATG_T Cuts at: 3469 5131 3469 Size: 1662 4570 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6232 PfoI T'CCnGG_A Cuts at: 3393 3393 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PstI C_TGCA'G Cuts at: 830 2530 3584 830 Size: 1700 1054 3478 Fragments arranged by size: 3478 1700 1054 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2114 3065 2114 Size: 951 5281 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SapI GCTCTTCn'nnn_ Cuts at: 2538 2538 Size: 6232 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SfcI C'TryA_G Cuts at: 826 1045 2526 3035 3120 3580 4715 5396 5587 Size: 219 1481 509 85 460 1135 681 191 Cuts at: 5587 826 Size: 1471 Fragments arranged by size: 1481 1471 1135 681 509 460 219 191 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4336 5237 5499 820 Size: 197 926 2393 901 262 1553 Fragments arranged by size: 2393 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 2182 2581 4156 5 Size: 47 2130 399 1575 2081 Fragments arranged by size: 2130 2081 1575 399 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2004 2826 3671 513 Size: 552 939 822 845 3074 Fragments arranged by size: 3074 939 845 822 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2115 4259 4276 4429 4614 5059 325 Size: 1255 535 2144 17 153 185 445 1498 Fragments arranged by size: 2144 1498 1255 535 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3388 4478 6190 Size: 274 80 33 51 1443 1411 1090 1712 Cuts at: 6190 96 Size: 138 Fragments arranged by size: 1712 1443 1411 1090 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 2182 4361 2182 Size: 2179 4053 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI