DDX1 ORF clone pFN21AB5738

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5738

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Clone info.

Species

human

Clone Name

pFN21AB5738 Sequence (with vector) Restriction Map

Product ID

FHC03715

Flexi Clone PID

FXC03715

ORF Sequence

DNA sequence (2220 bp)
Protein sequence (740 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527788]

Gene

KIBB5738

Symbol

DDX1

Alias : DBP-RB, UKVH5d

Description

DEAD (Asp-Glu-Ala-Asp) box helicase 1

Original Clone ID

cs01733

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2220 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 740 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1686.1 0 100.0 DDX1 CCDS6951.1 2.1e-17 25.0 DDX31 CCDS12577.1 7.4e-16 25.4 HNRNPUL1 CCDS82351.1 7.5e-16 25.4 HNRNPUL1 CCDS12576.1 8.3e-16 25.4 HNRNPUL1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 26 418 PF00270 DNA/RNA helicase IPR003877 130 245 PF00622 SPla/RYanodine receptor SPRY IPR001650 538 609 PF00271 Helicase HMMSmart IPR014001 21 444 SM00487 DEAD-like helicase IPR018355 130 246 SM00449 SPla/RYanodine receptor subgroup IPR001650 520 610 SM00490 Helicase ProfileScan IPR014014 2 30 PS51195 RNA helicase IPR001870 70 247 PS50188 B302/SPRY domain IPR014001 255 428 PS51192 DEAD-like helicase IPR001650 493 681 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004930 740 601257 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5738 Download

>KIBB5738  2220 bp
ATGGCGGCCTTCTCCGAGATGGGTGTAATGCCTGAGATTGCACAAGCTGTGGAAGAGATG
GATTGGCTCCTCCCAACTGATATCCAGGCTGAATCTATCCCATTGATCTTAGGAGGAGGT
GATGTACTTATGGCTGCAGAAACAGGAAGTGGCAAAACTGGTGCTTTTAGTATTCCAGTT
ATCCAGATAGTTTATGAAACTCTGAAAGACCAACAGGAAGGCAAAAAAGGAAAAACAACA
ATTAAAACTGGTGCTTCAGTGCTGAACAAATGGCAGATGAACCCATATGACAGAGGATCT
GCTTTTGCAATTGGGTCAGATGGTCTTTGTTGTCAAAGCAGAGAAGTAAAGGAATGGCAT
GGGTGTAGAGCTACTAAAGGATTAATGAAAGGGAAACACTACTATGAAGTATCCTGTCAT
GACCAAGGGTTATGCAGGGTCGGGTGGTCTACCATGCAGGCCTCTTTGGACCTAGGTACT
GACAAGTTTGGATTTGGCTTTGGTGGAACAGGAAAGAAATCCCATAACAAACAATTTGAT
AATTATGGAGAGGAATTCACTATGCATGATACCATTGGATGTTACCTGGATATAGATAAG
GGACATGTCAAGTTCTCCAAAAATGGAAAAGATCTTGGTCTGGCATTTGAAATACCACCA
CATATGAAAAACCAAGCCCTCTTTCCTGCCTGTGTTTTGAAGAATGCTGAACTGAAATTT
AACTTCGGTGAAGAGGAATTTAAGTTTCCACCAAAAGATGGCTTTGTTGCTCTTTCCAAG
GCACCGGATGGTTACATTGTCAAATCACAGCACTCAGGTAATGCACAGGTGACACAAACA
AAGTTTCTCCCCAATGCTCCGAAAGCTCTCATTGTTGAACCTTCCCGGGAGTTAGCTGAA
CAAACTTTGAATAACATCAAGCAGTTTAAGAAATACATTGATAATCCTAAATTAAGGGAG
CTTCTGATAATTGGAGGTGTTGCAGCCCGGGATCAGCTCTCTGTTTTGGAAAATGGAGTA
GATATAGTTGTAGGTACTCCGGGAAGACTAGATGACTTGGTGTCAACTGGAAAGCTGAAC
TTATCTCAAGTTAGATTCCTGGTCCTGGATGAAGCTGATGGGCTTCTTTCTCAAGGTTAT
TCTGATTTTATAAATAGGATGCACAATCAGATTCCTCAGGTTACCTCTGATGGAAAAAGA
CTTCAGGTGATTGTTTGCTCTGCCACTTTGCATTCTTTCGATGTAAAGAAACTGTCCGAG
AAGATAATGCATTTTCCTACATGGGTTGACTTAAAAGGAGAAGACTCTGTTCCAGATACT
GTACACCATGTTGTTGTCCCAGTAAATCCCAAAACTGACAGACTCTGGGAAAGGCTTGGA
AAGAGCCACATTAGAACTGATGATGTACATGCAAAAGATAACACAAGACCTGGTGCTAAT
AGTCCAGAGATGTGGTCTGAAGCTATTAAAATCCTGAAAGGGGAGTATGCTGTCCGGGCA
ATCAAGGAACATAAGATGGATCAAGCAATTATCTTCTGTAGAACCAAAATTGACTGTGAT
AACTTGGAGCAGTACTTTATACAACAAGGAGGAGGACCTGATAAAAAAGGACACCAGTTC
TCATGTGTTTGTCTTCATGGTGACAGAAAGCCTCATGAGAGAAAGCAAAACTTGGAAAGA
TTTAAGAAAGGAGATGTAAGATTCTTGATTTGCACAGATGTAGCTGCTAGAGGAATTGAT
ATCCACGGTGTTCCTTATGTTATAAATGTCACTCTGCCCGATGAAAAGCAAAACTACGTA
CATCGAATTGGCAGAGTAGGAAGAGCTGAAAGGATGGGTCTGGCAATTTCCCTGGTGGCA
ACAGAAAAAGAAAAGGTTTGGTACCATGTATGTAGCAGCCGTGGAAAAGGGTGTTATAAC
ACAAGACTCAAGGAAGATGGAGGCTGTACCATATGGTACAACGAGATGCAGTTACTATCT
GAGATAGAAGAACACCTGAACTGTACCATTTCTCAGGTTGAGCCGGATATAAAGGTACCA
GTGGATGAATTTGATGGGAAAGTTACCTACGGTCAGAAAAGGGCTGCTGGTGGTGGAAGC
TATAAAGGCCATGTGGATATTTTGGCACCTACTGTTCAAGAGTTGGCTGCCCTTGAAAAG
GAGGCGCAGACATCTTTCCTGCATCTTGGCTACCTTCCTAACCAGCTGTTCAGAACCTTC

ORF Protein

Cloned ORF protein sequence for pFN21AB5738 Download

>KIBB5738  740 aa
MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPV
IQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWH
GCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFD
NYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPPHMKNQALFPACVLKNAELKF
NFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQVTQTKFLPNAPKALIVEPSRELAE
QTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLENGVDIVVGTPGRLDDLVSTGKLN
LSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSE
KIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGAN
SPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQF
SCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYV
HRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLS
EIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEK
EAQTSFLHLGYLPNQLFRTF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5738 Download

>pFN21AB5738  6925 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCCTTCTCCGAGATGGGTGTAATGCCTGA
GATTGCACAAGCTGTGGAAGAGATGGATTGGCTCCTCCCAACTGATATCCAGGCTGAATC
TATCCCATTGATCTTAGGAGGAGGTGATGTACTTATGGCTGCAGAAACAGGAAGTGGCAA
AACTGGTGCTTTTAGTATTCCAGTTATCCAGATAGTTTATGAAACTCTGAAAGACCAACA
GGAAGGCAAAAAAGGAAAAACAACAATTAAAACTGGTGCTTCAGTGCTGAACAAATGGCA
GATGAACCCATATGACAGAGGATCTGCTTTTGCAATTGGGTCAGATGGTCTTTGTTGTCA
AAGCAGAGAAGTAAAGGAATGGCATGGGTGTAGAGCTACTAAAGGATTAATGAAAGGGAA
ACACTACTATGAAGTATCCTGTCATGACCAAGGGTTATGCAGGGTCGGGTGGTCTACCAT
GCAGGCCTCTTTGGACCTAGGTACTGACAAGTTTGGATTTGGCTTTGGTGGAACAGGAAA
GAAATCCCATAACAAACAATTTGATAATTATGGAGAGGAATTCACTATGCATGATACCAT
TGGATGTTACCTGGATATAGATAAGGGACATGTCAAGTTCTCCAAAAATGGAAAAGATCT
TGGTCTGGCATTTGAAATACCACCACATATGAAAAACCAAGCCCTCTTTCCTGCCTGTGT
TTTGAAGAATGCTGAACTGAAATTTAACTTCGGTGAAGAGGAATTTAAGTTTCCACCAAA
AGATGGCTTTGTTGCTCTTTCCAAGGCACCGGATGGTTACATTGTCAAATCACAGCACTC
AGGTAATGCACAGGTGACACAAACAAAGTTTCTCCCCAATGCTCCGAAAGCTCTCATTGT
TGAACCTTCCCGGGAGTTAGCTGAACAAACTTTGAATAACATCAAGCAGTTTAAGAAATA
CATTGATAATCCTAAATTAAGGGAGCTTCTGATAATTGGAGGTGTTGCAGCCCGGGATCA
GCTCTCTGTTTTGGAAAATGGAGTAGATATAGTTGTAGGTACTCCGGGAAGACTAGATGA
CTTGGTGTCAACTGGAAAGCTGAACTTATCTCAAGTTAGATTCCTGGTCCTGGATGAAGC
TGATGGGCTTCTTTCTCAAGGTTATTCTGATTTTATAAATAGGATGCACAATCAGATTCC
TCAGGTTACCTCTGATGGAAAAAGACTTCAGGTGATTGTTTGCTCTGCCACTTTGCATTC
TTTCGATGTAAAGAAACTGTCCGAGAAGATAATGCATTTTCCTACATGGGTTGACTTAAA
AGGAGAAGACTCTGTTCCAGATACTGTACACCATGTTGTTGTCCCAGTAAATCCCAAAAC
TGACAGACTCTGGGAAAGGCTTGGAAAGAGCCACATTAGAACTGATGATGTACATGCAAA
AGATAACACAAGACCTGGTGCTAATAGTCCAGAGATGTGGTCTGAAGCTATTAAAATCCT
GAAAGGGGAGTATGCTGTCCGGGCAATCAAGGAACATAAGATGGATCAAGCAATTATCTT
CTGTAGAACCAAAATTGACTGTGATAACTTGGAGCAGTACTTTATACAACAAGGAGGAGG
ACCTGATAAAAAAGGACACCAGTTCTCATGTGTTTGTCTTCATGGTGACAGAAAGCCTCA
TGAGAGAAAGCAAAACTTGGAAAGATTTAAGAAAGGAGATGTAAGATTCTTGATTTGCAC
AGATGTAGCTGCTAGAGGAATTGATATCCACGGTGTTCCTTATGTTATAAATGTCACTCT
GCCCGATGAAAAGCAAAACTACGTACATCGAATTGGCAGAGTAGGAAGAGCTGAAAGGAT
GGGTCTGGCAATTTCCCTGGTGGCAACAGAAAAAGAAAAGGTTTGGTACCATGTATGTAG
CAGCCGTGGAAAAGGGTGTTATAACACAAGACTCAAGGAAGATGGAGGCTGTACCATATG
GTACAACGAGATGCAGTTACTATCTGAGATAGAAGAACACCTGAACTGTACCATTTCTCA
GGTTGAGCCGGATATAAAGGTACCAGTGGATGAATTTGATGGGAAAGTTACCTACGGTCA
GAAAAGGGCTGCTGGTGGTGGAAGCTATAAAGGCCATGTGGATATTTTGGCACCTACTGT
TCAAGAGTTGGCTGCCCTTGAAAAGGAGGCGCAGACATCTTTCCTGCATCTTGGCTACCT
TCCTAACCAGCTGTTCAGAACCTTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT