(Circular) (Six-base) MAPSORT of: pFN21AB5738.seq Check: 5377 from: 1 to: 6925 pFN21AB5738 6925 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4735 278 Size: 53 83 186 936 3199 2468 Fragments arranged by size: 3199 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2453 4268 1937 Size: 516 1815 4594 Fragments arranged by size: 4594 1815 516 Acc65I G'GTAC_C Cuts at: 3885 4039 4246 3885 Size: 154 207 6564 Fragments arranged by size: 6564 207 154 AclI AA'CG_TT Cuts at: 1563 5052 5425 1563 Size: 3489 373 3063 Fragments arranged by size: 3489 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2244 3191 3483 4988 6372 6640 1632 Size: 612 947 292 1505 1384 268 1917 Fragments arranged by size: 1917 1505 1384 947 612 292 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6728 AflIII A'CryG_T Cuts at: 1203 2608 5824 6801 1203 Size: 1405 3216 977 1327 Fragments arranged by size: 3216 1405 1327 977 AgeI A'CCGG_T Cuts at: 5756 5756 Size: 6925 AhdI GACnn_n'nnGTC Cuts at: 3064 5654 3064 Size: 2590 4335 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6925 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6925 AlwNI CAG_nnn'CTG Cuts at: 3323 3369 4668 6240 3323 Size: 46 1299 1572 4008 Fragments arranged by size: 4008 1572 1299 46 ApaLI G'TGCA_C Cuts at: 4982 6138 4982 Size: 1156 5769 ApoI r'AATT_y Cuts at: 1493 2558 2720 2741 4052 4234 4460 4612 6721 Size: 1065 162 21 1311 182 226 152 2109 Cuts at: 6721 1493 Size: 1697 Fragments arranged by size: 2109 1697 1311 1065 226 182 162 152 21 AseI AT'TA_AT Cuts at: 160 1032 2387 5479 160 Size: 872 1355 3092 1606 Fragments arranged by size: 3092 1606 1355 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 AvaI C'yCGr_G Cuts at: 1870 1943 2889 2991 4250 1870 Size: 73 946 102 1259 4545 Fragments arranged by size: 4545 1259 946 102 73 AvrII C'CTAG_G Cuts at: 2476 2476 Size: 6925 BamHI G'GATC_C Cuts at: 4255 4622 4255 Size: 367 6558 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2785 3885 4039 4129 4246 Size: 325 217 627 998 1100 154 90 117 Cuts at: 4246 5701 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1100 998 627 325 217 154 117 90 BanII G_rGCy'C Cuts at: 1391 4244 1391 Size: 2853 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3055 3312 3629 928 Size: 971 1156 257 317 4224 Fragments arranged by size: 4224 1156 971 317 257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5116 5150 1903 Size: 34 3179 34 3678 Fragments arranged by size: 3678 3179 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2425 4817 6027 2425 Size: 2392 1210 3323 Fragments arranged by size: 3323 2392 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6925 BfrBI ATG'CAT Cuts at: 2569 3274 2569 Size: 705 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5536 136 Size: 107 122 71 631 605 337 3527 1525 Fragments arranged by size: 3527 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2635 6920 2635 Size: 4285 2640 BlpI GC'TnA_GC Cuts at: 4342 4342 Size: 6925 Bme1580I G_kGCm'C Cuts at: 1790 4986 6142 1790 Size: 3196 1156 2573 Fragments arranged by size: 3196 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6925 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3338 4656 5614 Size: 416 53 83 399 24 1921 1318 958 Cuts at: 5614 442 Size: 1753 Fragments arranged by size: 1921 1753 1318 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6925 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5585 1239 Size: 4346 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3075 3120 3917 5050 5915 6213 3075 Size: 45 797 1133 865 298 3787 Fragments arranged by size: 3787 1133 865 797 298 45 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5588 882 Size: 617 4089 2219 Fragments arranged by size: 4089 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3802 4716 493 Size: 711 2598 914 2702 Fragments arranged by size: 2702 2598 914 711 BsaBI GATnn'nnATC Cuts at: 6919 6919 Size: 6925 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4732 5114 5749 275 Size: 53 83 186 936 3199 382 635 1451 Fragments arranged by size: 3199 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2788 5358 5756 6030 6177 6588 2788 Size: 2570 398 274 147 411 3125 Fragments arranged by size: 3125 2570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2112 2142 1178 Size: 30 165 30 709 30 5961 Fragments arranged by size: 5961 709 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2133 3609 1186 Size: 877 70 1476 4502 Fragments arranged by size: 4502 1476 877 70 BseYI C'CCAG_C Cuts at: 6128 6624 6128 Size: 496 6429 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4375 5136 6164 1540 Size: 275 186 2374 761 1028 2301 Fragments arranged by size: 2374 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4986 5071 6142 4986 Size: 85 1071 5769 Fragments arranged by size: 5769 1071 85 BsmI GAATG_Cn' Cuts at: 2713 3235 4436 4529 2713 Size: 522 1201 93 5109 Fragments arranged by size: 5109 1201 522 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4244 4986 5071 6142 1391 Size: 399 2454 742 85 1071 2174 Fragments arranged by size: 2454 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6588 6588 Size: 6925 BspHI T'CATG_A Cuts at: 2422 3658 4814 6544 2422 Size: 1236 1156 1730 2803 Fragments arranged by size: 2803 1730 1236 1156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4280 844 Size: 3436 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4812 1394 Size: 429 2989 3507 Fragments arranged by size: 3507 2989 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5420 5594 65 Size: 1183 4172 174 1396 Fragments arranged by size: 4172 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5569 5756 1700 Size: 110 3759 187 2869 Fragments arranged by size: 3759 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3325 3409 96 Size: 3229 84 3612 Fragments arranged by size: 3612 3229 84 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6925 BssSI C'ACGA_G Cuts at: 4985 5997 4985 Size: 1012 5913 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6925 BstBI TT'CG_AA Cuts at: 5729 5729 Size: 6925 BstEII G'GTnAC_C Cuts at: 1184 3184 1184 Size: 2000 4925 BstXI CCAn_nnnn'nTGG Cuts at: 3456 3456 Size: 6925 BstYI r'GATC_y Cuts at: 1972 2300 2635 4255 4622 5007 5024 6476 6562 Size: 328 335 1620 367 385 17 1452 86 Cuts at: 6562 6574 6920 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1620 1452 385 367 346 335 328 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3180 3180 Size: 6925 BtgI C'CryG_G Cuts at: 513 1065 2004 3748 3904 513 Size: 552 939 1744 156 3534 Fragments arranged by size: 3534 1744 939 552 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6186 Fragments arranged by size: 6186 683 56 BtsI GCAGTG_nn' Cuts at: 4441 5235 5255 4441 Size: 794 20 6111 Fragments arranged by size: 6111 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6925 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6890 DraI TTT'AAA Cuts at: 4229 4229 Size: 6925 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5932 809 Size: 507 4616 1802 Fragments arranged by size: 4616 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4372 5261 6890 Size: 54 1425 50 275 18 2542 889 1629 Cuts at: 6890 8 Size: 43 Fragments arranged by size: 2542 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4372 1537 Size: 275 2560 4090 Fragments arranged by size: 4090 2560 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2730 3820 4855 1353 Size: 699 678 1090 1035 3423 Fragments arranged by size: 3423 1090 1035 699 678 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5512 5886 6032 387 Size: 1064 138 3923 374 146 1280 Fragments arranged by size: 3923 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 3191 3483 4988 5585 6372 6640 Size: 393 612 947 292 1505 597 787 268 Cuts at: 6640 1239 Size: 1524 Fragments arranged by size: 1524 1505 947 787 612 597 393 292 268 EcoNI CCTnn'n_nnAGG Cuts at: 4160 4160 Size: 6925 EcoO109I rG'GnC_Cy Cuts at: 3599 3599 Size: 6925 EcoRI G'AATT_C Cuts at: 2558 4234 4612 2558 Size: 1676 378 4871 Fragments arranged by size: 4871 1676 378 EcoRV GAT'ATC Cuts at: 2086 3745 2086 Size: 1659 5266 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3100 3691 3100 Size: 591 6334 FspI TGC'GCA Cuts at: 5431 5431 Size: 6925 HaeII r_GCGC'y Cuts at: 1678 6072 1678 Size: 4394 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2987 3019 3050 Size: 32 135 32 713 32 670 32 31 Cuts at: 3050 3082 3090 3122 3154 3186 3443 3475 5566 Size: 32 8 32 32 32 257 32 2091 Cuts at: 5566 5598 5640 5672 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2091 713 670 257 135 42 32 32 32 32 32 32 32 32 32 32 32 31 8 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3292 4269 4515 669 Size: 988 281 1131 223 977 246 3079 Fragments arranged by size: 3079 1131 988 977 281 246 223 HindIII A'AGCT_T Cuts at: 748 748 Size: 6925 HpaI GTT'AAC Cuts at: 4515 4515 Size: 6925 KpnI G_GTAC'C Cuts at: 3889 4043 4250 3889 Size: 154 207 6564 Fragments arranged by size: 6564 207 154 MfeI C'AATT_G Cuts at: 2313 4524 2313 Size: 2211 4714 MluI A'CGCG_T Cuts at: 6801 6801 Size: 6925 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6039 6223 1305 Size: 4734 184 2007 Fragments arranged by size: 4734 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5103 Fragments arranged by size: 5103 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2406 4883 5242 5401 518 Size: 648 1240 2477 359 159 2042 Fragments arranged by size: 2477 2042 1240 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4210 4341 5018 6166 6411 1606 Size: 2604 131 677 1148 245 2120 Fragments arranged by size: 2604 2120 1148 677 245 131 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5434 Fragments arranged by size: 5434 939 552 NdeI CA'TA_TG Cuts at: 387 2290 2667 3956 387 Size: 1903 377 1289 3356 Fragments arranged by size: 3356 1903 1289 377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6925 NotI GC'GGCC_GC Cuts at: 4372 4372 Size: 6925 NsiI A_TGCA'T Cuts at: 2571 3276 2571 Size: 705 6220 NspI r_CATG'y Cuts at: 1132 1229 2612 3416 4283 5828 1132 Size: 97 1383 804 867 1545 2229 Fragments arranged by size: 2229 1545 1383 867 804 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6925 PciI A'CATG_T Cuts at: 2608 5824 2608 Size: 3216 3709 PflMI CCAn_nnn'nTGG Cuts at: 1108 2762 1108 Size: 1654 5271 PfoI T'CCnGG_A Cuts at: 3043 3108 3043 Size: 65 6860 PmeI GTTT'AAAC Cuts at: 4229 4229 Size: 6925 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4994 5026 6507 6539 4994 Size: 32 1481 32 5380 Fragments arranged by size: 5380 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3599 3599 Size: 6925 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6925 PsiI TTA'TAA Cuts at: 3155 3767 3921 4495 3155 Size: 612 154 574 5585 Fragments arranged by size: 5585 612 574 154 PstI C_TGCA'G Cuts at: 830 2143 4277 830 Size: 1313 2134 3478 Fragments arranged by size: 3478 2134 1313 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6925 PvuII CAG'CTG Cuts at: 4210 4210 Size: 6925 SalI G'TCGA_C Cuts at: 1936 4267 1936 Size: 2331 4594 SapI GCTCTTCn'nnn_ Cuts at: 3820 3820 Size: 6925 SbfI CC_TGCA'GG Cuts at: 4277 4277 Size: 6925 ScaI AGT'ACT Cuts at: 3578 5173 6885 3578 Size: 1595 1712 3618 Fragments arranged by size: 3618 1712 1595 SexAI A'CCwGG_T Cuts at: 3433 3433 Size: 6925 SfcI C'TryA_G Cuts at: 826 1045 2139 3541 4273 5408 6089 6280 826 Size: 219 1094 1402 732 1135 681 191 1471 Fragments arranged by size: 1471 1402 1135 1094 732 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6925 SmaI CCC'GGG Cuts at: 1872 2891 2993 4252 1872 Size: 1019 102 1259 4545 Fragments arranged by size: 4545 1259 1019 102 SmlI C'TyrA_G Cuts at: 820 1017 1943 3090 3135 3932 5029 5930 6192 Size: 197 926 1147 45 797 1097 901 262 Cuts at: 6192 820 Size: 1553 Fragments arranged by size: 1553 1147 1097 926 901 797 262 197 45 SnaBI TAC'GTA Cuts at: 493 3802 4716 493 Size: 3309 914 2702 Fragments arranged by size: 3309 2702 914 SpeI A'CTAG_T Cuts at: 152 152 Size: 6925 SphI G_CATG'C Cuts at: 1132 4283 1132 Size: 3151 3774 SspI AAT'ATT Cuts at: 5 52 4849 5 Size: 47 4797 2081 Fragments arranged by size: 4797 2081 47 StuI AGG'CCT Cuts at: 1517 2465 1517 Size: 948 5977 StyI C'CwwG_G Cuts at: 513 1065 2004 2428 2476 2781 4364 513 Size: 552 939 424 48 305 1583 3074 Fragments arranged by size: 3074 1583 939 552 424 305 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2015 2355 4952 4969 5122 5307 5752 Size: 1255 435 340 2597 17 153 185 445 Cuts at: 5752 325 Size: 1498 Fragments arranged by size: 2597 1498 1255 445 435 340 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2128 3325 3409 Size: 274 80 33 51 1443 151 1197 84 Cuts at: 3409 3576 5171 6883 96 Size: 167 1595 1712 138 Fragments arranged by size: 1712 1595 1443 1197 274 167 151 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6885 6917 6885 Size: 32 6893 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6925 XbaI T'CTAG_A Cuts at: 4261 4261 Size: 6925 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6925 XmaI C'CCGG_G Cuts at: 1870 2889 2991 4250 1870 Size: 1019 102 1259 4545 Fragments arranged by size: 4545 1259 1019 102 XmnI GAAnn'nnTTC Cuts at: 5054 5054 Size: 6925 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4733 276 Size: 53 83 186 936 3199 2468 Fragments arranged by size: 3199 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI