MAN1A2 ORF clone pFN21AB5584

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5584

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Clone info.

Species

human

Clone Name

pFN21AB5584 Sequence (with vector) Restriction Map

Product ID

FHC01825

Flexi Clone PID

FXC01825

Original Clone PID

ORK01825

ORF Sequence

DNA sequence (1923 bp)
Protein sequence (641 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385126]

Gene

KIBB5584

Symbol

MAN1A2

Alias : MAN1B

Description

mannosidase, alpha, class 1A, member 2

Original Clone ID

ef06366

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1923 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 641 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS895.1 0 100.0 MAN1A2 CCDS5122.1 2.7e-130 63.8 MAN1A1 CCDS265.1 2.4e-110 52.4 MAN1C1 CCDS7029.1 1.4e-55 34.8 MAN1B1 CCDS13247.1 6e-15 33.5 EDEM2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001382 187 207 PR00747 Glycoside hydrolase IPR001382 224 238 PR00747 Glycoside hydrolase IPR001382 261 279 PR00747 Glycoside hydrolase IPR001382 300 319 PR00747 Glycoside hydrolase IPR001382 395 412 PR00747 Glycoside hydrolase IPR001382 456 472 PR00747 Glycoside hydrolase IPR001382 529 553 PR00747 Glycoside hydrolase IPR001382 590 610 PR00747 Glycoside hydrolase HMMPfam IPR001382 187 626 PF01532 Glycoside hydrolase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 35 EKFILLLILSAFITLCFGAFFFL 57 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006690 641 604345 100.0 100.0 XP_006710365 637 604345 100.0 99.1 XP_011538838 598 604345 100.0 93.1 NP_005898 653 604344 63.8 93.4 XP_005267043 736 604344 63.8 93.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5584 Download

>KIBB5584  1923 bp
ATGACTACCCCAGCCCTGCTGCCCCTCTCTGGACGTAGGATACCACCTCTGAACCTGGGG
CCGCCTTCCTTCCCACATCACAGGGCTACCTTGAGACTTTCTGAGAAGTTTATTCTTCTC
CTTATTCTTAGTGCCTTCATCACTCTGTGTTTTGGGGCATTCTTTTTCCTTCCAGACTCT
TCAAAACACAAACGCTTTGATTTGGGTTTAGAAGATGTGTTAATTCCACATGTAGATGCC
GGTAAAGGGGCTAAAAACCCCGGAGTCTTCCTGATCCATGGACCCGATGAACATAGACAC
AGGGAAGAGGAAGAACGTCTGAGAAATAAAATTCGAGCTGATCATGAGAAGGCCTTGGAA
GAAGCAAAAGAAAAATTAAGAAAGTCAAGAGAGGAAATTCGAGCAGAAATTCAGACAGAG
AAAAATAAGGTAGTCCAAGAAATGAAGATAAAAGAGAACAAGCCACTGCCACCAGTCCCT
ATTCCCAACCTTGTAGGAATACGTGGTGGAGACCCAGAAGATAATGACATAAGAGAGAAA
AGGGAAAAAATTAAAGAGATGATGAAACATGCTTGGGATAACTATAGGACATATGGGTGG
GGACATAATGAACTCAGACCTATTGCAAGGAAAGGACACTCCCCTAACATATTTGGAAGT
TCACAAATGGGTGCTACCATAGTAGATGCTTTGGATACCCTTTATATCATGGGACTTCAT
GATGAATTCCTAGATGGGCAAAGATGGATTGAAGACAACCTTGATTTCAGTGTGAATTCA
GAGGTGTCTGTGTTTGAAGTCAACATTCGATTTATTGGAGGCCTACTTGCAGCATATTAC
CTATCAGGAGAGGAGATATTCAAGATTAAAGCAGTGCAATTGGCTGAGAAACTCCTTCCT
GCCTTTAACACACCTACTGGGATTCCTTGGGCAATGGTGAATTTGAAAAGTGGAGTAGGG
CGAAACTGGGGCTGGGCATCTGCAGGTAGCAGCATTCTGGCTGAATTTGGTACACTACAT
ATGGAGTTCATCCACCTCAGCTACTTGACAGGGGACCTGACTTACTACAAAAAGGTTATG
CACATTCGGAAACTACTTCAGAAAATGGATCGTCCAAATGGTCTTTATCCAAATTATTTG
AACCCCAGAACAGGGCGCTGGGGTCAGTATCATACATCTGTCGGTGGCCTGGGAGACAGT
TTTTATGAATACTTACTGAAAGCATGGTTGATGTCAGATAAAACAGACCATGAGGCAAGA
AAGATGTATGATGATGCTATTGAGGCTATAGAAAAACATCTTATTAAGAAGTCTCGTGGA
GGTCTTACCTTTATTGGAGAATGGAAGAATGGGCACTTGGAAAAAAAGATGGGGCATTTG
GCCTGCTTTGCTGGGGGAATGTTTGCACTAGGAGCAGATGGTTCCAGAGCAGATAAAGCT
GGTCATTATTTAGAGCTAGGGGCAGAAATTGCACGTACTTGTCATGAGTCATATGACAGA
ACTGCATTAAAGCTAGGTCCTGAATCATTCAAGTTTGATGGTGCAGTGGAGGCTGTGGCT
GTCCGGCAGGCTGAAAAGTATTATATCCTCCGTCCAGAAGTAATTGAAACCTATTGGTAC
CTATGGCGATTCACTCACGATCCAAGATACAGGCAGTGGGGCTGGGAAGCAGCACTGGCC
ATTGAAAAGTATTGCCGAGTTAATGGTGGGTTTTCTGGAGTCAAAGATGTATATTCCTCT
ACTCCTACACATGATGATGTACAGCAGAGCTTTTTTCTTGCTGAAACATTAAAATATTTG
TATCTGCTGTTCTCCGGTGATGACCTTTTACCTTTAGACCACTGGGTGTTTAATACAGAG
GCTCACCCTCTGCCTGTGTTACATTTAGCCAACACCACACTTTCAGGTAATCCTGCTGTT
CGA

ORF Protein

Cloned ORF protein sequence for pFN21AB5584 Download

>KIBB5584  641 aa
MTTPALLPLSGRRIPPLNLGPPSFPHHRATLRLSEKFILLLILSAFITLCFGAFFFLPDS
SKHKRFDLGLEDVLIPHVDAGKGAKNPGVFLIHGPDEHRHREEEERLRNKIRADHEKALE
EAKEKLRKSREEIRAEIQTEKNKVVQEMKIKENKPLPPVPIPNLVGIRGGDPEDNDIREK
REKIKEMMKHAWDNYRTYGWGHNELRPIARKGHSPNIFGSSQMGATIVDALDTLYIMGLH
DEFLDGQRWIEDNLDFSVNSEVSVFEVNIRFIGGLLAAYYLSGEEIFKIKAVQLAEKLLP
AFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVM
HIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEAR
KMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKA
GHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWY
LWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYL
YLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5584 Download

>pFN21AB5584  6628 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACTACCCCAGCCCTGCTGCCCCTCTCTGGACG
TAGGATACCACCTCTGAACCTGGGGCCGCCTTCCTTCCCACATCACAGGGCTACCTTGAG
ACTTTCTGAGAAGTTTATTCTTCTCCTTATTCTTAGTGCCTTCATCACTCTGTGTTTTGG
GGCATTCTTTTTCCTTCCAGACTCTTCAAAACACAAACGCTTTGATTTGGGTTTAGAAGA
TGTGTTAATTCCACATGTAGATGCCGGTAAAGGGGCTAAAAACCCCGGAGTCTTCCTGAT
CCATGGACCCGATGAACATAGACACAGGGAAGAGGAAGAACGTCTGAGAAATAAAATTCG
AGCTGATCATGAGAAGGCCTTGGAAGAAGCAAAAGAAAAATTAAGAAAGTCAAGAGAGGA
AATTCGAGCAGAAATTCAGACAGAGAAAAATAAGGTAGTCCAAGAAATGAAGATAAAAGA
GAACAAGCCACTGCCACCAGTCCCTATTCCCAACCTTGTAGGAATACGTGGTGGAGACCC
AGAAGATAATGACATAAGAGAGAAAAGGGAAAAAATTAAAGAGATGATGAAACATGCTTG
GGATAACTATAGGACATATGGGTGGGGACATAATGAACTCAGACCTATTGCAAGGAAAGG
ACACTCCCCTAACATATTTGGAAGTTCACAAATGGGTGCTACCATAGTAGATGCTTTGGA
TACCCTTTATATCATGGGACTTCATGATGAATTCCTAGATGGGCAAAGATGGATTGAAGA
CAACCTTGATTTCAGTGTGAATTCAGAGGTGTCTGTGTTTGAAGTCAACATTCGATTTAT
TGGAGGCCTACTTGCAGCATATTACCTATCAGGAGAGGAGATATTCAAGATTAAAGCAGT
GCAATTGGCTGAGAAACTCCTTCCTGCCTTTAACACACCTACTGGGATTCCTTGGGCAAT
GGTGAATTTGAAAAGTGGAGTAGGGCGAAACTGGGGCTGGGCATCTGCAGGTAGCAGCAT
TCTGGCTGAATTTGGTACACTACATATGGAGTTCATCCACCTCAGCTACTTGACAGGGGA
CCTGACTTACTACAAAAAGGTTATGCACATTCGGAAACTACTTCAGAAAATGGATCGTCC
AAATGGTCTTTATCCAAATTATTTGAACCCCAGAACAGGGCGCTGGGGTCAGTATCATAC
ATCTGTCGGTGGCCTGGGAGACAGTTTTTATGAATACTTACTGAAAGCATGGTTGATGTC
AGATAAAACAGACCATGAGGCAAGAAAGATGTATGATGATGCTATTGAGGCTATAGAAAA
ACATCTTATTAAGAAGTCTCGTGGAGGTCTTACCTTTATTGGAGAATGGAAGAATGGGCA
CTTGGAAAAAAAGATGGGGCATTTGGCCTGCTTTGCTGGGGGAATGTTTGCACTAGGAGC
AGATGGTTCCAGAGCAGATAAAGCTGGTCATTATTTAGAGCTAGGGGCAGAAATTGCACG
TACTTGTCATGAGTCATATGACAGAACTGCATTAAAGCTAGGTCCTGAATCATTCAAGTT
TGATGGTGCAGTGGAGGCTGTGGCTGTCCGGCAGGCTGAAAAGTATTATATCCTCCGTCC
AGAAGTAATTGAAACCTATTGGTACCTATGGCGATTCACTCACGATCCAAGATACAGGCA
GTGGGGCTGGGAAGCAGCACTGGCCATTGAAAAGTATTGCCGAGTTAATGGTGGGTTTTC
TGGAGTCAAAGATGTATATTCCTCTACTCCTACACATGATGATGTACAGCAGAGCTTTTT
TCTTGCTGAAACATTAAAATATTTGTATCTGCTGTTCTCCGGTGATGACCTTTTACCTTT
AGACCACTGGGTGTTTAATACAGAGGCTCACCCTCTGCCTGTGTTACATTTAGCCAACAC
CACACTTTCAGGTAATCCTGCTGTTCGAGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT