(Circular) (Six-base) MAPSORT of: pFN21AB5584.seq Check: 9562 from: 1 to: 6628 pFN21AB5584 6628 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3971 1937 Size: 2034 4594 Acc65I G'GTAC_C Cuts at: 3621 3949 3621 Size: 328 6300 AclI AA'CG_TT Cuts at: 1563 4755 5128 1563 Size: 3192 373 3063 Fragments arranged by size: 3192 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3086 4691 6075 6343 1632 Size: 1454 1605 1384 268 1917 Fragments arranged by size: 1917 1605 1454 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 2233 5527 6504 1203 Size: 1030 3294 977 1327 Fragments arranged by size: 3294 1327 1030 977 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 5357 5357 Size: 6628 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6628 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6628 AlwNI CAG_nnn'CTG Cuts at: 4371 5943 4371 Size: 1572 5056 ApaLI G'TGCA_C Cuts at: 4685 5841 4685 Size: 1156 5472 ApoI r'AATT_y Cuts at: 1493 2334 2400 2412 2729 2779 2944 3008 3937 Size: 841 66 12 317 50 165 64 929 Cuts at: 3937 4163 4315 6424 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 841 317 226 165 152 66 64 50 12 AseI AT'TA_AT Cuts at: 160 1032 5182 160 Size: 872 4150 1606 Fragments arranged by size: 4150 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 3953 1870 Size: 73 2010 4545 Fragments arranged by size: 4545 2010 73 BamHI G'GATC_C Cuts at: 3958 4325 3958 Size: 367 6261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3621 3949 5404 618 Size: 325 217 627 1834 328 1455 1842 Fragments arranged by size: 1842 1834 1455 627 328 325 217 BanII G_rGCy'C Cuts at: 1391 3947 1391 Size: 2556 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2263 2763 928 Size: 971 364 500 4793 Fragments arranged by size: 4793 971 500 364 BbvCI CC'TCA_GC Cuts at: 3041 3041 Size: 6628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4819 4853 1903 Size: 34 2882 34 3678 Fragments arranged by size: 3678 2882 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2037 2692 4520 5730 2037 Size: 655 1828 1210 2935 Fragments arranged by size: 2935 1828 1210 655 BclI T'GATC_A Cuts at: 1548 2344 1548 Size: 796 5832 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5239 136 Size: 107 122 71 631 605 3567 1525 Fragments arranged by size: 3567 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6623 6623 Size: 6628 BlpI GC'TnA_GC Cuts at: 4045 4045 Size: 6628 Bme1580I G_kGCm'C Cuts at: 1790 3360 4689 5845 1790 Size: 1570 1329 1156 2573 Fragments arranged by size: 2573 1570 1329 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6628 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2931 2980 3856 Size: 416 53 83 399 24 1514 49 876 Cuts at: 3856 4359 5317 442 Size: 503 958 1753 Fragments arranged by size: 1753 1514 958 876 503 416 399 83 53 49 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3741 5288 1239 Size: 2502 1547 2579 Fragments arranged by size: 2579 2502 1547 Bpu10I CC'TnA_GC Cuts at: 3041 3041 Size: 6628 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2116 4753 5618 5916 2116 Size: 2637 865 298 2828 Fragments arranged by size: 2828 2637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2508 5291 882 Size: 617 1009 2783 2219 Fragments arranged by size: 2783 2219 1009 617 BsaAI yAC'GTr Cuts at: 493 1204 2507 3479 4419 493 Size: 711 1303 972 940 2702 Fragments arranged by size: 2702 1303 972 940 711 BsaBI GATnn'nnATC Cuts at: 6622 6622 Size: 6628 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4435 4817 5452 275 Size: 53 83 186 936 2902 382 635 1451 Fragments arranged by size: 2902 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3818 5061 5459 5733 5880 6291 3818 Size: 1243 398 274 147 411 4155 Fragments arranged by size: 4155 1243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6403 Fragments arranged by size: 6403 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2870 1186 Size: 1684 4944 BseYI C'CCAG_C Cuts at: 2014 2976 3162 3395 3666 5831 6327 2014 Size: 962 186 233 271 2165 496 2315 Fragments arranged by size: 2315 2165 962 496 271 233 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3567 1163 Size: 573 1831 4224 Fragments arranged by size: 4224 1831 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4078 4839 5867 1540 Size: 275 186 2077 761 1028 2301 Fragments arranged by size: 2301 2077 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4689 4774 5845 4689 Size: 85 1071 5472 Fragments arranged by size: 5472 1071 85 BsmI GAATG_Cn' Cuts at: 2162 2997 4139 4232 2162 Size: 835 1142 93 4558 Fragments arranged by size: 4558 1142 835 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3360 3947 4689 4774 5845 1391 Size: 399 1570 587 742 85 1071 2174 Fragments arranged by size: 2174 1570 1071 742 587 399 85 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 2347 2722 3487 4517 6247 2347 Size: 375 765 1030 1730 2728 Fragments arranged by size: 2728 1730 1030 765 375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2978 3983 844 Size: 2134 1005 3489 Fragments arranged by size: 3489 2134 1005 BsrBI CCG'CTC Cuts at: 1394 1823 4515 1394 Size: 429 2692 3507 Fragments arranged by size: 3507 2692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2943 5123 5297 65 Size: 1183 1695 2180 174 1396 Fragments arranged by size: 2180 1695 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 5272 5459 1700 Size: 110 433 3029 187 2869 Fragments arranged by size: 3029 2869 433 187 110 BsrGI T'GTAC_A Cuts at: 96 3763 96 Size: 3667 2961 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6628 BssSI C'ACGA_G Cuts at: 3318 4688 5700 3318 Size: 1370 1012 4246 Fragments arranged by size: 4246 1370 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3473 794 Size: 2679 3949 BstBI TT'CG_AA Cuts at: 5432 5432 Size: 6628 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6628 BstYI r'GATC_y Cuts at: 1972 3958 4325 4710 4727 6179 6265 6277 6623 Size: 1986 367 385 17 1452 86 12 346 Cuts at: 6623 1972 Size: 1977 Fragments arranged by size: 1986 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2281 513 Size: 552 1216 4860 Fragments arranged by size: 4860 1216 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5889 Fragments arranged by size: 5889 683 56 BtsI GCAGTG_nn' Cuts at: 2468 2883 3555 3665 4144 4938 4958 2468 Size: 415 672 110 479 794 20 4138 Fragments arranged by size: 4138 794 672 479 415 110 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6593 DraI TTT'AAA Cuts at: 3932 3932 Size: 6628 DraIII CAC_nnn'GTG Cuts at: 2151 3850 2151 Size: 1699 4929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5635 809 Size: 507 4319 1802 Fragments arranged by size: 4319 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3681 4075 4964 Size: 54 1425 50 275 18 1851 394 889 Cuts at: 4964 6593 8 Size: 1629 43 Fragments arranged by size: 1851 1629 1425 889 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4075 1537 Size: 275 2263 4090 Fragments arranged by size: 4090 2263 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2188 2304 4558 1353 Size: 835 116 2254 3423 Fragments arranged by size: 3423 2254 835 116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5215 5589 5735 387 Size: 1064 138 3626 374 146 1280 Fragments arranged by size: 3626 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3086 3741 4691 5288 6075 6343 1239 Size: 393 1454 655 950 597 787 268 1524 Fragments arranged by size: 1524 1454 950 787 655 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3058 3521 3058 Size: 463 6165 EcoRI G'AATT_C Cuts at: 2729 2779 3937 4315 2729 Size: 50 1158 378 5042 Fragments arranged by size: 5042 1158 378 50 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2780 2780 Size: 6628 FspI TGC'GCA Cuts at: 5134 5134 Size: 6628 HaeII r_GCGC'y Cuts at: 1678 3163 5775 1678 Size: 1485 2612 2531 Fragments arranged by size: 2612 2531 1485 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2035 2067 5269 5301 5343 Size: 32 135 32 463 32 3202 32 42 Cuts at: 5343 5375 1373 Size: 32 2626 Fragments arranged by size: 3202 2626 463 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2806 3972 4218 669 Size: 988 281 868 1166 246 3079 Fragments arranged by size: 3079 1166 988 868 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6628 HpaI GTT'AAC Cuts at: 4218 4218 Size: 6628 KpnI G_GTAC'C Cuts at: 3625 3953 3625 Size: 328 6300 MfeI C'AATT_G Cuts at: 2882 4227 2882 Size: 1345 5283 MluI A'CGCG_T Cuts at: 6504 6504 Size: 6628 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5742 5926 1305 Size: 4437 184 2007 Fragments arranged by size: 4437 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3683 10 Size: 54 1425 343 1851 2955 Fragments arranged by size: 2955 1851 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2599 3759 4586 4945 5104 518 Size: 648 1072 361 1160 827 359 159 2042 Fragments arranged by size: 2042 1160 1072 827 648 361 359 159 MspA1I CmG'CkG Cuts at: 1606 4044 4721 5869 6114 1606 Size: 2438 677 1148 245 2120 Fragments arranged by size: 2438 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2281 513 Size: 552 1216 4860 Fragments arranged by size: 4860 1216 552 NdeI CA'TA_TG Cuts at: 387 2596 3024 3496 387 Size: 2209 428 472 3519 Fragments arranged by size: 3519 2209 472 428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6628 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 2237 2576 3986 5531 1132 Size: 97 1008 339 1410 1545 2229 Fragments arranged by size: 2229 1545 1410 1008 339 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6628 PciI A'CATG_T Cuts at: 2233 5527 2233 Size: 3294 3334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6628 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4697 4729 6210 6242 4697 Size: 32 1481 32 5083 Fragments arranged by size: 5083 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3058 3521 3058 Size: 463 6165 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6628 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3797 3829 3797 Size: 32 6596 PstI C_TGCA'G Cuts at: 830 2989 3980 830 Size: 2159 991 3478 Fragments arranged by size: 3478 2159 991 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6628 SalI G'TCGA_C Cuts at: 1936 3970 1936 Size: 2034 4594 SbfI CC_TGCA'GG Cuts at: 3980 3980 Size: 6628 ScaI AGT'ACT Cuts at: 4876 6588 4876 Size: 1712 4916 SfcI C'TryA_G Cuts at: 826 1045 2587 2985 3291 3976 5111 5792 5983 Size: 219 1542 398 306 685 1135 681 191 Cuts at: 5983 826 Size: 1471 Fragments arranged by size: 1542 1471 1135 685 681 398 306 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6628 SmaI CCC'GGG Cuts at: 1872 3955 1872 Size: 2083 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2095 4732 5633 5895 820 Size: 197 926 152 2637 901 262 1553 Fragments arranged by size: 2637 1553 926 901 262 197 152 SnaBI TAC'GTA Cuts at: 493 4419 493 Size: 3926 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6628 SphI G_CATG'C Cuts at: 1132 3986 1132 Size: 2854 3774 SspI AAT'ATT Cuts at: 5 52 3800 4552 5 Size: 47 3748 752 2081 Fragments arranged by size: 3748 2081 752 47 StuI AGG'CCT Cuts at: 1517 2357 2826 1517 Size: 840 469 5319 Fragments arranged by size: 5319 840 469 StyI C'CwwG_G Cuts at: 513 1065 2281 2358 2930 4067 513 Size: 552 1216 77 572 1137 3074 Fragments arranged by size: 3074 1216 1137 572 552 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2589 2663 3838 4655 4672 4825 5010 Size: 1255 1009 74 1175 817 17 153 185 Cuts at: 5010 5455 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1175 1009 817 445 185 153 74 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3763 4874 6586 Size: 274 80 33 51 1443 1786 1111 1712 Cuts at: 6586 96 Size: 138 Fragments arranged by size: 1786 1712 1443 1111 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3628 3660 6588 6620 3628 Size: 32 2928 32 3636 Fragments arranged by size: 3636 2928 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6628 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6628 XmaI C'CCGG_G Cuts at: 1870 3953 1870 Size: 2083 4545 XmnI GAAnn'nnTTC Cuts at: 4757 4757 Size: 6628 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI