PREB ORF clone pFN21AB5408

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Halotag ORF Clone pFN21AB5408

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Clone info.

Species

human

Clone Name

pFN21AB5408 Sequence (with vector) Restriction Map

Product ID

FHC06476

Flexi Clone PID

FXC06476

Original Clone PID

ORK06476

ORF Sequence

DNA sequence (1251 bp)
Protein sequence (417 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463192]

Gene

KIBB5408

Symbol

PREB

Alias : SEC12

Description

prolactin regulatory element binding

Original Clone ID

bm04789

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1251 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 417 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1738.1 4.7e-150 100.0 PREB CCDS82431.1 2.7e-87 86.1 PREB The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019781 160 182 PF00400 WD40 repeat IPR019781 189 222 PF00400 WD40 repeat HMMSmart IPR001680 143 182 SM00320 WD40 repeat IPR001680 185 223 SM00320 WD40 repeat IPR001680 289 328 SM00320 WD40 repeat IPR001680 331 382 SM00320 WD40 repeat ProfileScan IPR019782 160 191 PS50082 WD40 repeat 2 IPR017986 160 232 PS50294 WD40-repeat-containing domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 314 FLGLGTVTGSVAIYIAFSLQCLY 336 PRIMARY 23 2 392 WLLLLLCVGLIIVTILLLQSAFP 414 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_037520 417 606395 100.0 100.0 NP_001317413 345 606395 100.0 82.7 NP_001317415 350 606395 99.1 80.6 NP_001317416 292 606395 81.8 80.6 NP_001317414 359 606395 86.1 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5408 Download

>KIBB5408  1251 bp
ATGGGCCGGCGCCGGGCGCCAGAGCTGTACCGGGCTCCGTTCCCGTTGTACGCGCTTCAG
GTCGACCCCAGCACTGGGCTGCTCATCGCTGCGGGCGGAGGAGGCGCCGCCAAGACAGGC
ATAAAGAATGGCGTGCACTTTCTGCAGCTAGAGCTGATTAATGGGCGCTTGAGTGCCTCC
TTGCTGCACTCCCATGACACAGAGACACGGGCCACCATGAACTTGGCACTGGCTGGTGAC
ATCCTTGCTGCAGGGCAGGATGCCCACTGTCAGCTCCTGCGCTTCCAGGCACATCAACAG
CAGGGCAACAAGGCAGAGAAGGCCGGTTCCAAGGAGCAGGGGCCTCGACAAAGGAAGGGA
GCAGCCCCAGCAGAGAAGAAATGTGGAGCGGAAACCCAGCACGAGGGGCTAGAACTCAGG
GTAGAGAATTTGCAGGCGGTGCAGACAGACTTTAGCTCCGATCCACTGCAGAAAGTTGTG
TGCTTCAACCACGATAATACCCTGCTTGCCACTGGAGGAACAGATGGCTACGTCCGTGTC
TGGAAGGTGCCCAGCCTGGAGAAGGTTCTGGAGTTCAAAGCCCACGAAGGGGAGATTGAA
GACCTGGCTTTAGGGCCTGATGGCAAGTTGGTAACCGTGGGCCGGGACCTTAAGGCCTCT
GTGTGGCAGAAGGATCAGCTGGTGACACAGCTGCACTGGCAAGAAAATGGACCCACCTTT
TCCAGCACACCTTACCGCTACCAGGCCTGCAGGTTTGGGCAGGTTCCAGACCAGCCTGCT
GGCCTGCGACTCTTCACAGTGCAAATTCCCCACAAGCGCCTGCGCCAGCCCCCTCCCTGC
TACCTCACAGCCTGGGATGGCTCCAACTTCTTGCCCCTTCGGACCAAGTCCTGTGGCCAT
GAAGTCGTCTCCTGCCTCGATGTCAGTGAATCCGGCACCTTCCTAGGCCTGGGCACAGTC
ACTGGCTCTGTTGCCATCTACATAGCTTTCTCTCTCCAGTGCCTCTACTACGTGAGGGAG
GCCCATGGCATTGTGGTGACGGATGTGGCCTTTCTACCTGAGAAGGGTCGTGGTCCAGAG
CTCCTTGGGTCCCATGAAACTGCCCTGTTCTCTGTGGCTGTGGACAGTCGTTGCCAGCTG
CATCTGTTGCCCTCACGGCGGAGTGTTCCTGTGTGGCTCCTGCTCCTGCTGTGTGTCGGG
CTTATTATTGTGACCATCCTGCTGCTCCAGAGTGCCTTTCCAGGTTTCCTT

ORF Protein

Cloned ORF protein sequence for pFN21AB5408 Download

>KIBB5408  417 aa
MGRRRAPELYRAPFPLYALQVDPSTGLLIAAGGGGAAKTGIKNGVHFLQLELINGRLSAS
LLHSHDTETRATMNLALAGDILAAGQDAHCQLLRFQAHQQQGNKAEKAGSKEQGPRQRKG
AAPAEKKCGAETQHEGLELRVENLQAVQTDFSSDPLQKVVCFNHDNTLLATGGTDGYVRV
WKVPSLEKVLEFKAHEGEIEDLALGPDGKLVTVGRDLKASVWQKDQLVTQLHWQENGPTF
SSTPYRYQACRFGQVPDQPAGLRLFTVQIPHKRLRQPPPCYLTAWDGSNFLPLRTKSCGH
EVVSCLDVSESGTFLGLGTVTGSVAIYIAFSLQCLYYVREAHGIVVTDVAFLPEKGRGPE
LLGSHETALFSVAVDSRCQLHLLPSRRSVPVWLLLLLCVGLIIVTILLLQSAFPGFL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5408 Download

>pFN21AB5408  5956 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCCGGCGCCGGGCGCCAGAGCTGTACCGGGC
TCCGTTCCCGTTGTACGCGCTTCAGGTCGACCCCAGCACTGGGCTGCTCATCGCTGCGGG
CGGAGGAGGCGCCGCCAAGACAGGCATAAAGAATGGCGTGCACTTTCTGCAGCTAGAGCT
GATTAATGGGCGCTTGAGTGCCTCCTTGCTGCACTCCCATGACACAGAGACACGGGCCAC
CATGAACTTGGCACTGGCTGGTGACATCCTTGCTGCAGGGCAGGATGCCCACTGTCAGCT
CCTGCGCTTCCAGGCACATCAACAGCAGGGCAACAAGGCAGAGAAGGCCGGTTCCAAGGA
GCAGGGGCCTCGACAAAGGAAGGGAGCAGCCCCAGCAGAGAAGAAATGTGGAGCGGAAAC
CCAGCACGAGGGGCTAGAACTCAGGGTAGAGAATTTGCAGGCGGTGCAGACAGACTTTAG
CTCCGATCCACTGCAGAAAGTTGTGTGCTTCAACCACGATAATACCCTGCTTGCCACTGG
AGGAACAGATGGCTACGTCCGTGTCTGGAAGGTGCCCAGCCTGGAGAAGGTTCTGGAGTT
CAAAGCCCACGAAGGGGAGATTGAAGACCTGGCTTTAGGGCCTGATGGCAAGTTGGTAAC
CGTGGGCCGGGACCTTAAGGCCTCTGTGTGGCAGAAGGATCAGCTGGTGACACAGCTGCA
CTGGCAAGAAAATGGACCCACCTTTTCCAGCACACCTTACCGCTACCAGGCCTGCAGGTT
TGGGCAGGTTCCAGACCAGCCTGCTGGCCTGCGACTCTTCACAGTGCAAATTCCCCACAA
GCGCCTGCGCCAGCCCCCTCCCTGCTACCTCACAGCCTGGGATGGCTCCAACTTCTTGCC
CCTTCGGACCAAGTCCTGTGGCCATGAAGTCGTCTCCTGCCTCGATGTCAGTGAATCCGG
CACCTTCCTAGGCCTGGGCACAGTCACTGGCTCTGTTGCCATCTACATAGCTTTCTCTCT
CCAGTGCCTCTACTACGTGAGGGAGGCCCATGGCATTGTGGTGACGGATGTGGCCTTTCT
ACCTGAGAAGGGTCGTGGTCCAGAGCTCCTTGGGTCCCATGAAACTGCCCTGTTCTCTGT
GGCTGTGGACAGTCGTTGCCAGCTGCATCTGTTGCCCTCACGGCGGAGTGTTCCTGTGTG
GCTCCTGCTCCTGCTGTGTGTCGGGCTTATTATTGTGACCATCCTGCTGCTCCAGAGTGC
CTTTCCAGGTTTCCTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT