(Circular) (Six-base) MAPSORT of: pFN21AB5408.seq Check: 5924 from: 1 to: 5956 pFN21AB5408 5956 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 3299 1937 Size: 130 1232 4594 Fragments arranged by size: 4594 1232 130 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 4019 5403 5671 1632 Size: 413 1974 1384 268 1917 Fragments arranged by size: 1974 1917 1384 413 268 AflII C'TTAA_G Cuts at: 820 1017 2654 820 Size: 197 1637 4122 Fragments arranged by size: 4122 1637 197 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2099 1184 Size: 915 5041 AlwNI CAG_nnn'CTG Cuts at: 2281 2966 3699 5271 2281 Size: 685 733 1572 2966 Fragments arranged by size: 2966 1572 733 685 ApaLI G'TGCA_C Cuts at: 2138 4013 5169 2138 Size: 1875 1156 2925 Fragments arranged by size: 2925 1875 1156 ApoI r'AATT_y Cuts at: 1493 2431 2808 3265 3491 3643 5752 1493 Size: 938 377 457 226 152 2109 1697 Fragments arranged by size: 2109 1697 938 457 377 226 152 AseI AT'TA_AT Cuts at: 160 1032 2163 4510 160 Size: 872 1131 2347 1606 Fragments arranged by size: 2347 1606 1131 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 AvrII C'CTAG_G Cuts at: 2947 2947 Size: 5956 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2013 2020 2108 2551 2939 Size: 325 217 627 226 7 88 443 388 Cuts at: 2939 3277 4732 618 Size: 338 1455 1842 Fragments arranged by size: 1842 1455 627 443 388 338 325 226 217 88 7 BanII G_rGCy'C Cuts at: 1391 2041 3087 3275 1391 Size: 650 1046 188 4072 Fragments arranged by size: 4072 1046 650 188 BbeI G_GCGC'C Cuts at: 2017 2024 2112 2017 Size: 7 88 5861 Fragments arranged by size: 5861 88 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2610 928 Size: 971 711 4274 Fragments arranged by size: 4274 971 711 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2330 2364 4147 4181 1903 Size: 34 393 34 1783 34 3678 Fragments arranged by size: 3678 1783 393 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3160 4567 136 Size: 107 122 71 631 605 1488 1407 1525 Fragments arranged by size: 1525 1488 1407 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2142 2556 2960 4017 5173 1790 Size: 352 414 404 1057 1156 2573 Fragments arranged by size: 2573 1156 1057 414 404 352 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2088 3687 4645 Size: 416 53 83 399 24 671 1599 958 Cuts at: 4645 442 Size: 1753 Fragments arranged by size: 1753 1599 958 671 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2538 2582 2594 2984 3215 4616 1239 Size: 1299 44 12 390 231 1401 2579 Fragments arranged by size: 2579 1401 1299 390 231 44 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2194 4081 4946 5244 2194 Size: 1887 865 298 2906 Fragments arranged by size: 2906 1887 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3016 3747 493 Size: 711 1812 731 2702 Fragments arranged by size: 2702 1812 731 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2014 2021 2109 3763 Size: 53 83 186 936 481 7 88 1654 Cuts at: 3763 4145 4780 275 Size: 382 635 1451 Fragments arranged by size: 1654 1451 936 635 481 382 186 88 83 53 7 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 1178 Size: 30 165 30 1041 30 4660 Fragments arranged by size: 4660 1041 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2118 1186 Size: 932 5024 BseYI C'CCAG_C Cuts at: 2072 2371 2400 2555 5159 5655 2072 Size: 299 29 155 2604 496 2373 Fragments arranged by size: 2604 2373 496 299 155 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2465 2681 1163 Size: 573 438 291 216 4438 Fragments arranged by size: 4438 573 438 291 216 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 3087 4017 4102 5173 2142 Size: 945 930 85 1071 2925 Fragments arranged by size: 2925 1071 945 930 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 BsmBI CGTCTCn'nnnn_ Cuts at: 2917 2917 Size: 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2142 2556 2960 3087 3275 4017 Size: 399 251 101 414 404 127 188 742 Cuts at: 4017 4102 5173 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 414 404 399 251 188 127 101 85 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2745 2755 3311 844 Size: 1901 10 556 3489 Fragments arranged by size: 3489 1901 556 10 BsrBI CCG'CTC Cuts at: 1394 1823 2393 3843 1394 Size: 429 570 1450 3507 Fragments arranged by size: 3507 1450 570 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2010 2327 4600 4787 1700 Size: 110 200 317 2273 187 2869 Fragments arranged by size: 2869 2273 317 200 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 2405 4016 5028 2405 Size: 1611 1012 3333 Fragments arranged by size: 3333 1611 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2146 2443 3151 794 Size: 1352 297 708 3599 Fragments arranged by size: 3599 1352 708 297 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 2635 1184 Size: 1451 4505 BstXI CCAn_nnnn'nTGG Cuts at: 2227 2783 2227 Size: 556 5400 BstYI r'GATC_y Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951 Size: 1314 367 385 17 1452 86 12 346 Cuts at: 5951 1972 Size: 1977 Fragments arranged by size: 1977 1452 1314 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2640 3028 513 Size: 552 939 636 388 3441 Fragments arranged by size: 3441 939 636 552 388 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2074 487 Size: 683 56 848 4369 Fragments arranged by size: 4369 848 683 56 BtsI GCAGTG_nn' Cuts at: 2468 3472 4266 4286 2468 Size: 1004 794 20 4138 Fragments arranged by size: 4138 1004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2878 2913 529 Size: 35 2314 35 3572 Fragments arranged by size: 3572 2314 35 35 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2899 3403 4292 Size: 54 1425 50 275 18 1069 504 889 Cuts at: 4292 5921 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1069 889 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2801 3886 1353 Size: 1448 1085 3423 Fragments arranged by size: 3423 1448 1085 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2115 3178 4543 4917 5063 387 Size: 1064 138 526 1063 1365 374 146 1280 Fragments arranged by size: 1365 1280 1064 1063 526 374 146 138 EcoICRI GAG'CTC Cuts at: 3085 3085 Size: 5956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2538 2582 2594 2984 3215 4019 Size: 393 413 493 44 12 390 231 804 Cuts at: 4019 4616 5403 5671 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 493 413 393 390 268 231 44 12 EcoNI CCTnn'n_nnAGG Cuts at: 2564 2564 Size: 5956 EcoO109I rG'GnC_Cy Cuts at: 2345 2618 2650 3093 2345 Size: 273 32 443 5208 Fragments arranged by size: 5208 443 273 32 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2017 2024 2112 2173 2824 5103 1678 Size: 339 7 88 61 651 2279 2531 Fragments arranged by size: 2531 2279 651 339 88 61 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 4597 4629 4671 Size: 32 135 32 872 32 2121 32 42 Cuts at: 4671 4703 1373 Size: 32 2626 Fragments arranged by size: 2626 2121 872 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 3300 3546 669 Size: 988 281 130 1232 246 3079 Fragments arranged by size: 3079 1232 988 281 246 130 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KasI G'GCGC_C Cuts at: 2013 2020 2108 2013 Size: 7 88 5861 Fragments arranged by size: 5861 88 7 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2892 5070 5254 1305 Size: 1587 2178 184 2007 Fragments arranged by size: 2178 2007 1587 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2901 10 Size: 54 1425 343 1069 3065 Fragments arranged by size: 3065 1425 1069 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2196 3038 3914 4273 4432 518 Size: 648 1030 842 876 359 159 2042 Fragments arranged by size: 2042 1030 876 842 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2683 2695 3142 3372 4049 5197 5442 1606 Size: 1077 12 447 230 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 677 447 245 230 12 NaeI GCC'GGC Cuts at: 2012 2012 Size: 5956 NarI GG'CG_CC Cuts at: 2014 2021 2109 2014 Size: 7 88 5861 Fragments arranged by size: 5861 88 7 NcoI C'CATG_G Cuts at: 513 1065 2004 3028 513 Size: 552 939 1024 3441 Fragments arranged by size: 3441 1024 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2010 2010 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2650 3093 2650 Size: 443 5513 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PstI C_TGCA'G Cuts at: 830 2151 2257 2475 2756 3308 830 Size: 1321 106 218 281 552 3478 Fragments arranged by size: 3478 1321 552 281 218 106 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2683 2695 3142 2683 Size: 12 447 5497 Fragments arranged by size: 5497 447 12 SacI G_AGCT'C Cuts at: 3087 3087 Size: 5956 SalI G'TCGA_C Cuts at: 1936 2066 3298 1936 Size: 130 1232 4594 Fragments arranged by size: 4594 1232 130 SanDI GG'GwC_CC Cuts at: 3093 3093 Size: 5956 SbfI CC_TGCA'GG Cuts at: 2756 3308 2756 Size: 552 5404 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 2147 2253 2471 2752 3304 4439 5120 Size: 219 1102 106 218 281 552 1135 681 Cuts at: 5120 5311 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1102 681 552 281 219 218 191 106 SfoI GGC'GCC Cuts at: 2015 2022 2110 2015 Size: 7 88 5861 Fragments arranged by size: 5861 88 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2173 2654 4060 4961 5223 820 Size: 197 926 230 481 1406 901 262 1553 Fragments arranged by size: 1553 1406 926 901 481 262 230 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 2660 2750 2952 1517 Size: 1143 90 202 4521 Fragments arranged by size: 4521 1143 202 90 StyI C'CwwG_G Cuts at: 513 1065 2004 2334 2947 3028 3088 3395 513 Size: 552 939 330 613 81 60 307 3074 Fragments arranged by size: 3074 939 613 552 330 307 81 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3983 4000 4153 4338 4783 325 Size: 1255 2403 17 153 185 445 1498 Fragments arranged by size: 2403 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 2890 1316 Size: 1574 4382 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2224 2224 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI