ZNF8 ORF clone pFN21AB5066

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Halotag ORF Clone pFN21AB5066

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Clone info.

Species

human

Clone Name

pFN21AB5066 Sequence (with vector) Restriction Map

Product ID

FHC01460

Flexi Clone PID

FXC01460

Original Clone PID

ORK01460

ORF Sequence

DNA sequence (1725 bp)
Protein sequence (575 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385025]

Gene

KIBB5066

Symbol

ZNF8

Alias : HF.18, Zfp128

Description

zinc finger protein 8

Original Clone ID

bm05643

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1725 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 575 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12974.1 1.3e-139 100.0 ZNF8 CCDS12504.1 5.3e-38 40.2 ZNF570 CCDS74355.1 5.6e-38 40.2 ZNF570 CCDS2011.1 2.8e-36 43.6 ZNF514 CCDS452.2 1.2e-35 39.7 ZFP69B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001909 25 65 PF01352 Krueppel-associated box IPR007087 257 279 PF00096 Zinc finger IPR007087 285 307 PF00096 Zinc finger IPR007087 341 363 PF00096 Zinc finger IPR007087 369 391 PF00096 Zinc finger IPR007087 397 419 PF00096 Zinc finger IPR007087 467 489 PF00096 Zinc finger HMMSmart IPR001909 25 85 SM00349 Krueppel-associated box IPR015880 257 279 SM00355 Zinc finger IPR015880 285 307 SM00355 Zinc finger IPR015880 313 335 SM00355 Zinc finger IPR015880 341 363 SM00355 Zinc finger IPR015880 369 391 SM00355 Zinc finger IPR015880 397 419 SM00355 Zinc finger IPR015880 467 489 SM00355 Zinc finger ProfileScan IPR001909 25 96 PS50805 Krueppel-associated box IPR007087 257 284 PS50157 Zinc finger IPR007087 285 312 PS50157 Zinc finger IPR007087 313 340 PS50157 Zinc finger IPR007087 341 368 PS50157 Zinc finger IPR007087 369 396 PS50157 Zinc finger IPR007087 397 424 PS50157 Zinc finger IPR007087 467 494 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_066575 575 194532 100.0 100.0 XP_011525495 549 194545 39.4 90.1 XP_016882567 549 194545 39.4 90.1 XP_016882568 549 194545 39.4 90.1 XP_011533137 457 604082 36.6 80.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5066 Download

>KIBB5066  1725 bp
ATGGACCCCGAGGACGAAGGGGTAGCGGGAGTGATGTCTGTGGGGCCGCCGGCGGCCCGG
CTTCAGGAACCAGTGACCTTCCGGGATGTGGCTGTGGACTTTACCCAGGAGGAATGGGGG
CAGCTGGACCCTACCCAGAGGATCCTCTACCGTGACGTGATGCTGGAGACCTTTGGTCAC
CTGCTCTCCATAGGTCCTGAGCTTCCGAAGCCTGAAGTCATCTCCCAGCTGGAGCAAGGG
ACCGAGCTATGGGTGGCTGAGAGAGGAACCACCCAGGGCTGCCATCCAGCCTGGGAGCCT
CGATCTGAAAGCCAAGCATCACGCAAGGAAGAGGGCCTGCCTGAAGAGGAGCCATCCCAT
GTCACGGGAAGGGAAGGATTCCCGACAGATGCTCCTTATCCCACCACGTTAGGGAAAGAC
AGGGAGTGTCAGAGCCAGAGTCTGGCACTCAAGGAGCAGAATAACTTGAAGCAGTTGGAA
TTTGGCCTCAAGGAAGCACCAGTTCAAGATCAAGGCTACAAAACTCTCAGACTCAGGGAA
AACTGCGTCCTGAGTTCAAGCCCAAATCCATTCCCAGAGATCTCTAGAGGGGAGTATTTG
TATACTTACGACTCACAGATTACAGACTCAGAACATAACTCCAGCTTAGTCAGTCAGCAG
ACAGGCTCCCCAGGAAAACAGCCCGGTGAAAACAGTGACTGTCACAGAGATTCCAGTCAG
GCCATTCCAATTACGGAACTCACAAAAAGCCAGGTGCAGGACAAACCCTACAAATGTACT
GACTGTGGGAAGTCGTTTAACCATAACGCACACCTCACCGTGCACAAGAGGATTCATACG
GGAGAAAGACCTTATATGTGCAAGGAGTGTGGGAAAGCCTTCAGCCAGAACTCCTCCCTC
GTCCAGCATGAGCGCATCCACACTGGAGACAAGCCCTACAAGTGTGCCGAATGTGGGAAG
TCTTTCTGCCATAGTACACACCTTACCGTCCATCGGAGGATTCACACTGGGGAGAAGCCC
TATGAGTGTCAGGACTGTGGGAGGGCCTTCAACCAGAACTCCTCCCTGGGGCGGCACAAG
AGGACACACACTGGGGAGAAGCCATACACCTGCAGTGTGTGTGGGAAATCCTTCTCTCGG
ACCACTTGCCTTTTCCTGCACCTGAGAACTCACACCGAGGAGAGGCCCTACGAGTGTAAC
CACTGCGGGAAGGGCTTCAGGCACAGCTCATCCCTGGCCCAGCACCAGCGGAAGCACGCG
GGGGAGAAGCCCTTTGAGTGCCGCCAGAGGCTGATCTTTGAGCAGACGCCAGCTCTCACA
AAGCATGAATGGACAGAAGCCCTGGGCTGTGACCCACCTTTGAGTCAAGATGAGAGGACT
CACCGAAGCGACAGACCCTTCAAATGTAATCAGTGTGGGAAGTGTTTCATTCAGAGCTCT
CACCTCATCCGGCACCAGATAACTCACACCAGAGAGGAGCAGCCCCATGGGCGAAGCCGG
CGGCGTGAACAATCCTCGAGCAGGAACTCACACCTGGTTCAGCATCAACACCCGAACTCC
AGAAAGAGCTCTGCAGGCGGAGCAAAGGCAGGGCAGCCGGAAAGCAGAGCCCTGGCTTTG
TTTGACATCCAAAAAATCATGCAAGAGAAAAACCCTGTGCACGTTATTGGGGTGGAAGAG
CCTTCTGTGGGTGCTTCCATGTTATTTGACATCAGAGAATCCACA

ORF Protein

Cloned ORF protein sequence for pFN21AB5066 Download

>KIBB5066  575 aa
MDPEDEGVAGVMSVGPPAARLQEPVTFRDVAVDFTQEEWGQLDPTQRILYRDVMLETFGH
LLSIGPELPKPEVISQLEQGTELWVAERGTTQGCHPAWEPRSESQASRKEEGLPEEEPSH
VTGREGFPTDAPYPTTLGKDRECQSQSLALKEQNNLKQLEFGLKEAPVQDQGYKTLRLRE
NCVLSSSPNPFPEISRGEYLYTYDSQITDSEHNSSLVSQQTGSPGKQPGENSDCHRDSSQ
AIPITELTKSQVQDKPYKCTDCGKSFNHNAHLTVHKRIHTGERPYMCKECGKAFSQNSSL
VQHERIHTGDKPYKCAECGKSFCHSTHLTVHRRIHTGEKPYECQDCGRAFNQNSSLGRHK
RTHTGEKPYTCSVCGKSFSRTTCLFLHLRTHTEERPYECNHCGKGFRHSSSLAQHQRKHA
GEKPFECRQRLIFEQTPALTKHEWTEALGCDPPLSQDERTHRSDRPFKCNQCGKCFIQSS
HLIRHQITHTREEQPHGRSRRREQSSSRNSHLVQHQHPNSRKSSAGGAKAGQPESRALAL
FDIQKIMQEKNPVHVIGVEEPSVGASMLFDIREST

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5066 Download

>pFN21AB5066  6430 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACCCCGAGGACGAAGGGGTAGCGGGAGTGAT
GTCTGTGGGGCCGCCGGCGGCCCGGCTTCAGGAACCAGTGACCTTCCGGGATGTGGCTGT
GGACTTTACCCAGGAGGAATGGGGGCAGCTGGACCCTACCCAGAGGATCCTCTACCGTGA
CGTGATGCTGGAGACCTTTGGTCACCTGCTCTCCATAGGTCCTGAGCTTCCGAAGCCTGA
AGTCATCTCCCAGCTGGAGCAAGGGACCGAGCTATGGGTGGCTGAGAGAGGAACCACCCA
GGGCTGCCATCCAGCCTGGGAGCCTCGATCTGAAAGCCAAGCATCACGCAAGGAAGAGGG
CCTGCCTGAAGAGGAGCCATCCCATGTCACGGGAAGGGAAGGATTCCCGACAGATGCTCC
TTATCCCACCACGTTAGGGAAAGACAGGGAGTGTCAGAGCCAGAGTCTGGCACTCAAGGA
GCAGAATAACTTGAAGCAGTTGGAATTTGGCCTCAAGGAAGCACCAGTTCAAGATCAAGG
CTACAAAACTCTCAGACTCAGGGAAAACTGCGTCCTGAGTTCAAGCCCAAATCCATTCCC
AGAGATCTCTAGAGGGGAGTATTTGTATACTTACGACTCACAGATTACAGACTCAGAACA
TAACTCCAGCTTAGTCAGTCAGCAGACAGGCTCCCCAGGAAAACAGCCCGGTGAAAACAG
TGACTGTCACAGAGATTCCAGTCAGGCCATTCCAATTACGGAACTCACAAAAAGCCAGGT
GCAGGACAAACCCTACAAATGTACTGACTGTGGGAAGTCGTTTAACCATAACGCACACCT
CACCGTGCACAAGAGGATTCATACGGGAGAAAGACCTTATATGTGCAAGGAGTGTGGGAA
AGCCTTCAGCCAGAACTCCTCCCTCGTCCAGCATGAGCGCATCCACACTGGAGACAAGCC
CTACAAGTGTGCCGAATGTGGGAAGTCTTTCTGCCATAGTACACACCTTACCGTCCATCG
GAGGATTCACACTGGGGAGAAGCCCTATGAGTGTCAGGACTGTGGGAGGGCCTTCAACCA
GAACTCCTCCCTGGGGCGGCACAAGAGGACACACACTGGGGAGAAGCCATACACCTGCAG
TGTGTGTGGGAAATCCTTCTCTCGGACCACTTGCCTTTTCCTGCACCTGAGAACTCACAC
CGAGGAGAGGCCCTACGAGTGTAACCACTGCGGGAAGGGCTTCAGGCACAGCTCATCCCT
GGCCCAGCACCAGCGGAAGCACGCGGGGGAGAAGCCCTTTGAGTGCCGCCAGAGGCTGAT
CTTTGAGCAGACGCCAGCTCTCACAAAGCATGAATGGACAGAAGCCCTGGGCTGTGACCC
ACCTTTGAGTCAAGATGAGAGGACTCACCGAAGCGACAGACCCTTCAAATGTAATCAGTG
TGGGAAGTGTTTCATTCAGAGCTCTCACCTCATCCGGCACCAGATAACTCACACCAGAGA
GGAGCAGCCCCATGGGCGAAGCCGGCGGCGTGAACAATCCTCGAGCAGGAACTCACACCT
GGTTCAGCATCAACACCCGAACTCCAGAAAGAGCTCTGCAGGCGGAGCAAAGGCAGGGCA
GCCGGAAAGCAGAGCCCTGGCTTTGTTTGACATCCAAAAAATCATGCAAGAGAAAAACCC
TGTGCACGTTATTGGGGTGGAAGAGCCTTCTGTGGGTGCTTCCATGTTATTTGACATCAG
AGAATCCACAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT