(Circular) (Six-base) MAPSORT of: pFN21AB5066.seq Check: 1718 from: 1 to: 6430 pFN21AB5066 6430 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2193 3122 2193 Size: 929 5501 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2606 3773 1937 Size: 669 1167 4594 Fragments arranged by size: 4594 1167 669 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 2238 2367 2869 3205 4493 5877 6145 Size: 419 187 129 502 336 1288 1384 268 Cuts at: 6145 1632 Size: 1917 Fragments arranged by size: 1917 1384 1288 502 419 336 268 187 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 2178 5159 2178 Size: 2981 3449 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6430 AlwNI CAG_nnn'CTG Cuts at: 2446 2703 3295 4173 5745 2446 Size: 257 592 878 1572 3131 Fragments arranged by size: 3131 1572 878 592 257 ApaLI G'TGCA_C Cuts at: 2825 3662 4487 5643 2825 Size: 837 825 1156 3612 Fragments arranged by size: 3612 1156 837 825 ApoI r'AATT_y Cuts at: 1493 2483 3739 3965 4117 6226 1493 Size: 990 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 990 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2012 3520 3755 1870 Size: 73 69 1508 235 4545 Fragments arranged by size: 4545 1508 235 73 69 BamHI G'GATC_C Cuts at: 2145 3760 4127 2145 Size: 1615 367 4448 Fragments arranged by size: 4448 1615 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3456 3751 5206 618 Size: 325 217 627 1669 295 1455 1842 Fragments arranged by size: 1842 1669 1455 627 325 295 217 BanII G_rGCy'C Cuts at: 1391 3443 3575 3616 3749 1391 Size: 2052 132 41 133 4072 Fragments arranged by size: 4072 2052 133 132 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5459 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4621 4655 1903 Size: 34 2684 34 3678 Fragments arranged by size: 3678 2684 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3493 5041 136 Size: 107 122 71 631 605 1821 1548 1525 Fragments arranged by size: 1821 1548 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2583 6425 2583 Size: 3842 2588 BlpI GC'TnA_GC Cuts at: 3847 3847 Size: 6430 Bme1580I G_kGCm'C Cuts at: 1790 2829 3666 4491 5647 1790 Size: 1039 837 825 1156 2573 Fragments arranged by size: 2573 1156 1039 837 825 BmgBI CAC'GTC Cuts at: 1318 2161 1318 Size: 843 5587 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3021 3105 4161 Size: 416 53 83 399 24 1604 84 1056 Cuts at: 4161 5119 442 Size: 958 1753 Fragments arranged by size: 1753 1604 1056 958 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2255 2629 2949 3547 5090 1239 Size: 950 66 374 320 598 1543 2579 Fragments arranged by size: 2579 1543 950 598 374 320 66 Bpu10I CC'TnA_GC Cuts at: 2202 2202 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2438 2477 4555 5420 5718 2438 Size: 39 2078 865 298 3150 Fragments arranged by size: 3150 2078 865 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2165 5093 882 Size: 617 666 2928 2219 Fragments arranged by size: 2928 2219 666 617 BsaAI yAC'GTr Cuts at: 493 1204 4221 493 Size: 711 3017 2702 Fragments arranged by size: 3017 2702 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3311 4237 4619 5254 Size: 53 83 186 936 1778 926 382 635 Cuts at: 5254 275 Size: 1451 Fragments arranged by size: 1778 1451 936 926 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4863 5261 5535 5682 6093 4863 Size: 398 274 147 411 5200 Fragments arranged by size: 5200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 2380 2410 2588 Size: 30 165 30 771 30 176 30 178 Cuts at: 2588 2618 3047 3077 3545 3575 1178 Size: 30 429 30 468 30 4033 Fragments arranged by size: 4033 771 468 429 178 176 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 2887 3055 3197 3494 1186 Size: 1180 521 168 142 297 4122 Fragments arranged by size: 4122 1180 521 297 168 142 BseYI C'CCAG_C Cuts at: 2229 3243 5633 6129 2229 Size: 1014 2390 496 2530 Fragments arranged by size: 2530 2390 1014 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2780 3146 1163 Size: 573 1044 366 4447 Fragments arranged by size: 4447 1044 573 366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3880 4641 5669 1540 Size: 275 186 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2829 3443 3575 3666 4491 4576 5647 2829 Size: 614 132 91 825 85 1071 3612 Fragments arranged by size: 3612 1071 825 614 132 91 85 BsmI GAATG_Cn' Cuts at: 3941 4034 3941 Size: 93 6337 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2829 3443 3575 3616 3666 3749 4491 Size: 399 1039 614 132 41 50 83 742 Cuts at: 4491 4576 5647 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1039 742 614 399 132 85 83 50 41 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2193 3122 3785 844 Size: 1349 929 663 3489 Fragments arranged by size: 3489 1349 929 663 BsrBI CCG'CTC Cuts at: 1394 1823 4317 1394 Size: 429 2494 3507 Fragments arranged by size: 3507 2494 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2053 3501 5074 5261 1700 Size: 110 243 1448 1573 187 2869 Fragments arranged by size: 2869 1573 1448 243 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6430 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 2180 1184 Size: 996 5434 BstYI r'GATC_y Cuts at: 1972 2145 2583 3760 4127 4512 4529 5981 6067 Size: 173 438 1177 367 385 17 1452 86 Cuts at: 6067 6079 6425 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1177 438 385 367 346 173 86 17 12 BstZ17I GTA'TAC Cuts at: 2607 2607 Size: 6430 BtgI C'CryG_G Cuts at: 513 1065 2004 3490 513 Size: 552 939 1486 3453 Fragments arranged by size: 3453 1486 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 3124 3205 3946 4740 4760 3124 Size: 81 741 794 20 4794 Fragments arranged by size: 4794 794 741 81 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2854 2889 529 Size: 35 2290 35 4070 Fragments arranged by size: 4070 2290 35 35 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3877 4766 6395 Size: 54 1425 50 275 18 2047 889 1629 Cuts at: 6395 8 Size: 43 Fragments arranged by size: 2047 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3877 1537 Size: 275 2065 4090 Fragments arranged by size: 4090 2065 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 2343 3675 4360 1353 Size: 975 15 1332 685 3423 Fragments arranged by size: 3423 1332 975 685 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3597 5017 5391 5537 387 Size: 1064 138 2008 1420 374 146 1280 Fragments arranged by size: 2008 1420 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3441 3573 3441 Size: 132 6298 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2189 2238 2255 2367 2629 2869 Size: 393 419 138 49 17 112 262 240 Cuts at: 2869 2949 3205 3547 4493 5090 5877 6145 1239 Size: 80 256 342 946 597 787 268 1524 Fragments arranged by size: 1524 946 787 597 419 393 342 268 262 256 240 138 112 80 49 17 EcoNI CCTnn'n_nnAGG Cuts at: 3523 3523 Size: 6430 EcoO109I rG'GnC_Cy Cuts at: 2198 2338 3048 3189 2198 Size: 140 710 141 5439 Fragments arranged by size: 5439 710 141 140 EcoRI G'AATT_C Cuts at: 3739 4117 3739 Size: 378 6052 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 5577 1678 Size: 3899 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2196 2291 2323 2342 Size: 32 135 32 592 32 95 32 19 Cuts at: 2342 2374 2380 2412 2428 2460 3301 3333 5071 Size: 32 6 32 16 32 841 32 1738 Cuts at: 5071 5103 5145 5177 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1738 841 592 135 95 42 32 32 32 32 32 32 32 32 32 32 19 16 6 HincII GTy'rAC Cuts at: 669 1657 1938 3774 4020 669 Size: 988 281 1836 246 3079 Fragments arranged by size: 3079 1836 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 5544 5728 1305 Size: 1155 3084 184 2007 Fragments arranged by size: 3084 2007 1155 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4608 Fragments arranged by size: 4608 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4388 4747 4906 518 Size: 648 3222 359 159 2042 Fragments arranged by size: 3222 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2233 3253 3846 4523 5671 5916 1606 Size: 522 105 1020 593 677 1148 245 2120 Fragments arranged by size: 2120 1148 1020 677 593 522 245 105 NaeI GCC'GGC Cuts at: 2055 3503 2055 Size: 1448 4982 NcoI C'CATG_G Cuts at: 513 1065 2004 3490 513 Size: 552 939 1486 3453 Fragments arranged by size: 3453 1486 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NgoMIV G'CCGG_C Cuts at: 2053 3501 2053 Size: 1448 4982 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 3788 5333 1132 Size: 97 2559 1545 2229 Fragments arranged by size: 2559 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2278 3070 3346 1348 Size: 930 792 276 4432 Fragments arranged by size: 4432 930 792 276 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 2446 1108 Size: 1338 5092 PfoI T'CCnGG_A Cuts at: 2085 2085 Size: 6430 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2198 2198 Size: 6430 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspXI vC'TCGA_Gb Cuts at: 3520 3520 Size: 6430 PstI C_TGCA'G Cuts at: 830 3119 3580 3782 830 Size: 2289 461 202 3478 Fragments arranged by size: 3478 2289 461 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2128 2233 2128 Size: 105 6325 SacI G_AGCT'C Cuts at: 3443 3575 3443 Size: 132 6298 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SapI GCTCTTCn'nnn_ Cuts at: 3675 3675 Size: 6430 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SexAI A'CCwGG_T Cuts at: 3537 3537 Size: 6430 SfcI C'TryA_G Cuts at: 826 1045 3115 3576 3778 4913 5594 5785 826 Size: 219 2070 461 202 1135 681 191 1471 Fragments arranged by size: 2070 1471 1135 681 461 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 2053 1700 Size: 353 6077 SmaI CCC'GGG Cuts at: 1872 3757 1872 Size: 1885 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 2492 3520 4534 5435 5697 Size: 197 926 510 39 1028 1014 901 262 Cuts at: 5697 820 Size: 1553 Fragments arranged by size: 1553 1028 1014 926 901 510 262 197 39 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6430 StyI C'CwwG_G Cuts at: 513 1065 2004 3490 3869 513 Size: 552 939 1486 379 3074 Fragments arranged by size: 3074 1486 939 552 379 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 2261 2291 3683 4457 4474 4627 Size: 1255 665 16 30 1392 774 17 153 Cuts at: 4627 4812 5257 325 Size: 185 445 1498 Fragments arranged by size: 1498 1392 1255 774 665 445 185 153 30 17 16 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2780 2978 4676 Size: 274 80 33 51 1443 803 198 1698 Cuts at: 4676 6388 96 Size: 1712 138 Fragments arranged by size: 1712 1698 1443 803 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2174 2206 2699 2731 3218 3250 3545 3577 6390 Size: 32 493 32 487 32 295 32 2813 Cuts at: 6390 6422 2174 Size: 32 2182 Fragments arranged by size: 2813 2182 493 487 295 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 2588 3766 2588 Size: 1178 5252 XhoI C'TCGA_G Cuts at: 1943 3520 1943 Size: 1577 4853 XmaI C'CCGG_G Cuts at: 1870 3755 1870 Size: 1885 4545 XmnI GAAnn'nnTTC Cuts at: 2882 2966 3134 3218 3428 4559 2882 Size: 84 168 84 210 1131 4753 Fragments arranged by size: 4753 1131 210 168 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI