ANKS1B ORF clone pFN21AB4374

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Halotag ORF Clone pFN21AB4374

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Clone info.

Species

human

Clone Name

pFN21AB4374 Sequence (with vector) Restriction Map

Product ID

FHC11525

Flexi Clone PID

FXC11525

Original Clone PID

ORK11525

ORF Sequence

DNA sequence (1242 bp)
Protein sequence (414 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529098]

Gene

KIBB4374

Symbol

ANKS1B

Alias : AIDA, AIDA-1, ANKS2, EB-1, EB1, cajalin-2

Description

ankyrin repeat and sterile alpha motif domain containing 1B, transcript variant 3

Original Clone ID

fh22735

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1242 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 414 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS55866.1 1e-173 100.0 ANKS1B CCDS55867.1 8.6e-164 96.8 ANKS1B CCDS55871.1 3.8e-148 100.0 ANKS1B CCDS55864.1 3.2e-91 95.4 ANKS1B CCDS55872.1 1.1e-90 86.2 ANKS1B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR021129 37 99 PF00536 Sterile alpha motif IPR021129 110 169 PF00536 Sterile alpha motif IPR006020 228 358 PF00640 Phosphotyrosine interaction domain HMMSmart IPR001660 33 102 SM00454 Sterile alpha motif domain IPR001660 107 175 SM00454 Sterile alpha motif domain IPR006020 223 361 SM00462 Phosphotyrosine interaction domain ProfileScan IPR001660 39 102 PS50105 Sterile alpha motif domain IPR001660 110 169 PS50105 Sterile alpha motif domain IPR006020 225 358 PS01179 Phosphotyrosine interaction domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_064525 414 607815 100.0 100.0 NP_001190999 426 607815 96.8 98.3 XP_016875154 425 607815 100.0 94.2 XP_006719577 1184 607815 99.0 93.5 XP_016875147 1188 607815 99.7 92.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4374 Download

>KIBB4374  1242 bp
ATGATGTGGCAATGCCACCTCTCAGCCCAGGACTACCGCTATTACCCCGTGGACGGCTAC
TCCCTGCTTAAACGCTTCCCTCTTCATCCTCTTACAGGACCCAGATGCCCTGTCCAAACA
GTGGGACAATGGTTGGAAAGCATTGGGCTACCTCAGTACGAGAACCACCTGATGGCTAAT
GGATTTGACAATGTGCAGTTTATGGGAAGCAATGTTATGGAAGATCAGGATTTGTTGGAA
ATTGGAATCCTTAATTCTGGGCACAGACAAAGAATTCTACAGGCAATCCAGCTCCTTCCA
AAGATGAGACCCATTGGGCATGATGGCTACCATCCCACCTCTGTAGCTGAGTGGCTGGAT
TCCATTGAACTGGGCGACTACACCAAAGCCTTTCTAATTAATGGCTACACTTCGATGGAC
CTGTTGAAAAAAATCTGGGAGGTTGAACTTATTAATGTTTTAAAAATCAATTTGATTGGC
CACAGGAAACGTATTTTGGCATCTCTGGGAGACAGGCTGCACGACGATCCCCCACAGAAG
CCCCCTCGGTCCATCACCCTCAGGACAGGAGACTGGGGAGAACCTTCCATTACCTTGCGA
CCTCCGAATGAAGCCACAGCCTCTACCCCGGTACAGTACTGGCAGCATCACCCAGAAAAG
CTTATCTTCCAGTCGTGTGATTACAAAGCTTTTTATTTAGGTTCTATGCTGATAAAAGAG
CTTAGGGGGACAGAATCAACCCAAGATGCTTGTGCAAAAATGCGGGCTAACTGTCAGAAG
TCTACAGAGCAAATGAAGAAGGTCCCTACTATTATTCTTTCTGTCTCATATAAAGGAGTC
AAATTTATTGATGCAACAAATAAGAACATAATTGCTGAGCATGAAATTCGTAATATCTCC
TGTGCTGCCCAGGACCCAGAAGACCTCTCAACATTTGCCTATATCACAAAAGATTTGAAG
TCTAATCACCACTACTGTCATGTGTTTACTGCCTTTGATGTGAATTTAGCCTATGAAATC
ATCCTAACCCTGGGACAGGCATTCGAAGTCGCTTACCAGCTAGCACTACAAGCAAGAAAA
GGGGGACACTCCTCCACACTTCCAGAAAGCTTTGAAAACAAACCCTCCAAACCCATCCCC
AAGCCCCGCGTTAGCATTCGCAAGTCCGTGGATCTTCTTCATGCCAGCCATACCGGGCAG
GAACCATCAGAGAGACACACTGAGGAGGCACTCAGAAAATTT

ORF Protein

Cloned ORF protein sequence for pFN21AB4374 Download

>KIBB4374  414 aa
MMWQCHLSAQDYRYYPVDGYSLLKRFPLHPLTGPRCPVQTVGQWLESIGLPQYENHLMAN
GFDNVQFMGSNVMEDQDLLEIGILNSGHRQRILQAIQLLPKMRPIGHDGYHPTSVAEWLD
SIELGDYTKAFLINGYTSMDLLKKIWEVELINVLKINLIGHRKRILASLGDRLHDDPPQK
PPRSITLRTGDWGEPSITLRPPNEATASTPVQYWQHHPEKLIFQSCDYKAFYLGSMLIKE
LRGTESTQDACAKMRANCQKSTEQMKKVPTIILSVSYKGVKFIDATNKNIIAEHEIRNIS
CAAQDPEDLSTFAYITKDLKSNHHYCHVFTAFDVNLAYEIILTLGQAFEVAYQLALQARK
GGHSSTLPESFENKPSKPIPKPRVSIRKSVDLLHASHTGQEPSERHTEEALRKF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4374 Download

>pFN21AB4374  5947 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGTGGCAATGCCACCTCTCAGCCCAGGACTA
CCGCTATTACCCCGTGGACGGCTACTCCCTGCTTAAACGCTTCCCTCTTCATCCTCTTAC
AGGACCCAGATGCCCTGTCCAAACAGTGGGACAATGGTTGGAAAGCATTGGGCTACCTCA
GTACGAGAACCACCTGATGGCTAATGGATTTGACAATGTGCAGTTTATGGGAAGCAATGT
TATGGAAGATCAGGATTTGTTGGAAATTGGAATCCTTAATTCTGGGCACAGACAAAGAAT
TCTACAGGCAATCCAGCTCCTTCCAAAGATGAGACCCATTGGGCATGATGGCTACCATCC
CACCTCTGTAGCTGAGTGGCTGGATTCCATTGAACTGGGCGACTACACCAAAGCCTTTCT
AATTAATGGCTACACTTCGATGGACCTGTTGAAAAAAATCTGGGAGGTTGAACTTATTAA
TGTTTTAAAAATCAATTTGATTGGCCACAGGAAACGTATTTTGGCATCTCTGGGAGACAG
GCTGCACGACGATCCCCCACAGAAGCCCCCTCGGTCCATCACCCTCAGGACAGGAGACTG
GGGAGAACCTTCCATTACCTTGCGACCTCCGAATGAAGCCACAGCCTCTACCCCGGTACA
GTACTGGCAGCATCACCCAGAAAAGCTTATCTTCCAGTCGTGTGATTACAAAGCTTTTTA
TTTAGGTTCTATGCTGATAAAAGAGCTTAGGGGGACAGAATCAACCCAAGATGCTTGTGC
AAAAATGCGGGCTAACTGTCAGAAGTCTACAGAGCAAATGAAGAAGGTCCCTACTATTAT
TCTTTCTGTCTCATATAAAGGAGTCAAATTTATTGATGCAACAAATAAGAACATAATTGC
TGAGCATGAAATTCGTAATATCTCCTGTGCTGCCCAGGACCCAGAAGACCTCTCAACATT
TGCCTATATCACAAAAGATTTGAAGTCTAATCACCACTACTGTCATGTGTTTACTGCCTT
TGATGTGAATTTAGCCTATGAAATCATCCTAACCCTGGGACAGGCATTCGAAGTCGCTTA
CCAGCTAGCACTACAAGCAAGAAAAGGGGGACACTCCTCCACACTTCCAGAAAGCTTTGA
AAACAAACCCTCCAAACCCATCCCCAAGCCCCGCGTTAGCATTCGCAAGTCCGTGGATCT
TCTTCATGCCAGCCATACCGGGCAGGAACCATCAGAGAGACACACTGAGGAGGCACTCAG
AAAATTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT