GPR22 ORF clone pFN21AB4126

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Halotag ORF Clone pFN21AB4126

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Clone info.

Species

human

Clone Name

pFN21AB4126 Sequence (with vector) Restriction Map

Product ID

FHC01367

Flexi Clone PID

FXC01367

ORF Sequence

DNA sequence (1299 bp)
Protein sequence (433 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384903]

Gene

KIBB4126

Symbol

GPR22

Description

G protein-coupled receptor 22

Original Clone ID

fk03274

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1299 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 433 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5744.1 9.5e-149 100.0 GPR22 CCDS3438.1 3.6e-13 23.9 CCKAR The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 43 67 PR00237 GPCR IPR000276 77 98 PR00237 GPCR IPR000276 122 144 PR00237 GPCR IPR000276 156 177 PR00237 GPCR IPR000276 212 235 PR00237 GPCR IPR000276 311 335 PR00237 GPCR IPR000276 349 375 PR00237 GPCR HMMPfam IPR000276 59 367 PF00001 GPCR ProfileScan IPR017452 58 367 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 44 LTGFLMLEIVLGLGSNLTVLVLY 66 PRIMARY 23 2 79 IITMNLHVLDVIICVGCIPLTIV 101 PRIMARY 23 3 116 CFHEACVSFASVSTAINVFAIT 137 SECONDARY 22 4 158 VMLMISIWIFSFFSFLIPFIEVN 180 PRIMARY 23 5 214 LVQIPIFFFTVVVMLITYTKILQ 236 PRIMARY 23 6 315 SLLIISTFLLCWTPISVLNTTIL 337 PRIMARY 23 7 349 RLCFLVMAYGTTIFHPLLYAFTR 371 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005286 433 601910 100.0 100.0 XP_011514358 433 601910 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4126 Download

>KIBB4126  1299 bp
ATGTGTTTTTCTCCCATTCTGGAAATCAACATGCAGTCTGAATCTAACATTACAGTGCGA
GATGACATTGATGACATCAACACCAATATGTACCAACCACTATCATATCCGTTAAGCTTT
CAAGTGTCTCTCACCGGATTTCTTATGTTAGAAATTGTGTTGGGACTTGGCAGCAACCTC
ACTGTATTGGTACTTTACTGCATGAAATCCAACTTAATCAACTCTGTCAGTAACATTATT
ACAATGAATCTTCATGTACTTGATGTAATAATTTGTGTGGGATGTATTCCTCTAACTATA
GTTATCCTTCTGCTTTCACTGGAGAGTAACACTGCTCTCATTTGCTGTTTCCATGAGGCT
TGTGTATCTTTTGCAAGTGTCTCAACAGCAATCAACGTTTTTGCTATCACTTTGGACAGA
TATGACATCTCTGTAAAACCTGCAAACCGAATTCTGACAATGGGCAGAGCTGTAATGTTA
ATGATATCCATTTGGATTTTTTCTTTTTTCTCTTTCCTGATTCCTTTTATTGAGGTAAAT
TTTTTCAGTCTTCAAAGTGGAAATACCTGGGAAAACAAGACACTTTTATGTGTCAGTACA
AATGAATACTACACTGAACTGGGAATGTATTATCACCTGTTAGTACAGATCCCAATATTC
TTTTTCACTGTTGTAGTAATGTTAATCACATACACCAAAATACTTCAGGCTCTTAATATT
CGAATAGGCACAAGATTTTCAACAGGGCAGAAGAAGAAAGCAAGAAAGAAAAAGACAATT
TCTCTAACCACACAACATGAGGCTACAGACATGTCACAAAGCAGTGGTGGGAGAAATGTA
GTCTTTGGTGTAAGAACTTCAGTTTCTGTAATAATTGCCCTCCGGCGAGCTGTGAAACGA
CACCGTGAACGACGAGAAAGACAAAAGAGAGTCTTCAGGATGTCTTTATTGATTATTTCT
ACATTTCTTCTCTGCTGGACACCAATTTCTGTTTTAAATACCACCATTTTATGTTTAGGC
CCAAGTGACCTTTTAGTAAAATTAAGATTGTGTTTTTTAGTCATGGCTTATGGAACAACT
ATATTTCACCCTCTATTATATGCATTCACTAGACAAAAATTTCAAAAGGTCTTGAAAAGT
AAAATGAAAAAGCGAGTTGTTTCTATAGTAGAAGCTGATCCCCTGCCTAATAATGCTGTA
ATACACAACTCTTGGATAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGATAGTGAA
ATAAGAGAAAAATGTTTAGTGCCTCAGGTTGTCACAGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB4126 Download

>KIBB4126  433 aa
MCFSPILEINMQSESNITVRDDIDDINTNMYQPLSYPLSFQVSLTGFLMLEIVLGLGSNL
TVLVLYCMKSNLINSVSNIITMNLHVLDVIICVGCIPLTIVILLLSLESNTALICCFHEA
CVSFASVSTAINVFAITLDRYDISVKPANRILTMGRAVMLMISIWIFSFFSFLIPFIEVN
FFSLQSGNTWENKTLLCVSTNEYYTELGMYYHLLVQIPIFFFTVVVMLITYTKILQALNI
RIGTRFSTGQKKKARKKKTISLTTQHEATDMSQSSGGRNVVFGVRTSVSVIIALRRAVKR
HRERRERQKRVFRMSLLIISTFLLCWTPISVLNTTILCLGPSDLLVKLRLCFLVMAYGTT
IFHPLLYAFTRQKFQKVLKSKMKKRVVSIVEADPLPNNAVIHNSWIDPKRNKKITFEDSE
IREKCLVPQVVTD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4126 Download

>pFN21AB4126  6004 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGTTTTTCTCCCATTCTGGAAATCAACATGCA
GTCTGAATCTAACATTACAGTGCGAGATGACATTGATGACATCAACACCAATATGTACCA
ACCACTATCATATCCGTTAAGCTTTCAAGTGTCTCTCACCGGATTTCTTATGTTAGAAAT
TGTGTTGGGACTTGGCAGCAACCTCACTGTATTGGTACTTTACTGCATGAAATCCAACTT
AATCAACTCTGTCAGTAACATTATTACAATGAATCTTCATGTACTTGATGTAATAATTTG
TGTGGGATGTATTCCTCTAACTATAGTTATCCTTCTGCTTTCACTGGAGAGTAACACTGC
TCTCATTTGCTGTTTCCATGAGGCTTGTGTATCTTTTGCAAGTGTCTCAACAGCAATCAA
CGTTTTTGCTATCACTTTGGACAGATATGACATCTCTGTAAAACCTGCAAACCGAATTCT
GACAATGGGCAGAGCTGTAATGTTAATGATATCCATTTGGATTTTTTCTTTTTTCTCTTT
CCTGATTCCTTTTATTGAGGTAAATTTTTTCAGTCTTCAAAGTGGAAATACCTGGGAAAA
CAAGACACTTTTATGTGTCAGTACAAATGAATACTACACTGAACTGGGAATGTATTATCA
CCTGTTAGTACAGATCCCAATATTCTTTTTCACTGTTGTAGTAATGTTAATCACATACAC
CAAAATACTTCAGGCTCTTAATATTCGAATAGGCACAAGATTTTCAACAGGGCAGAAGAA
GAAAGCAAGAAAGAAAAAGACAATTTCTCTAACCACACAACATGAGGCTACAGACATGTC
ACAAAGCAGTGGTGGGAGAAATGTAGTCTTTGGTGTAAGAACTTCAGTTTCTGTAATAAT
TGCCCTCCGGCGAGCTGTGAAACGACACCGTGAACGACGAGAAAGACAAAAGAGAGTCTT
CAGGATGTCTTTATTGATTATTTCTACATTTCTTCTCTGCTGGACACCAATTTCTGTTTT
AAATACCACCATTTTATGTTTAGGCCCAAGTGACCTTTTAGTAAAATTAAGATTGTGTTT
TTTAGTCATGGCTTATGGAACAACTATATTTCACCCTCTATTATATGCATTCACTAGACA
AAAATTTCAAAAGGTCTTGAAAAGTAAAATGAAAAAGCGAGTTGTTTCTATAGTAGAAGC
TGATCCCCTGCCTAATAATGCTGTAATACACAACTCTTGGATAGATCCTAAAAGAAACAA
AAAAATTACCTTTGAAGATAGTGAAATAAGAGAAAAATGTTTAGTGCCTCAGGTTGTCAC
AGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT