(Circular) (Six-base) MAPSORT of: pFN21AB4126.seq Check: 7289 from: 1 to: 6004 pFN21AB4126 6004 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 2400 4131 4504 1563 Size: 837 1731 373 3063 Fragments arranged by size: 3063 1731 837 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2693 2847 2923 4067 5451 5719 1632 Size: 1061 154 76 1144 1384 268 1917 Fragments arranged by size: 1917 1384 1144 1061 268 154 76 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 2814 4903 5880 1203 Size: 1611 2089 977 1327 Fragments arranged by size: 2089 1611 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 2870 3747 5319 2870 Size: 877 1572 3555 Fragments arranged by size: 3555 1572 877 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 2454 2542 3122 3313 3539 3691 5800 1493 Size: 961 88 580 191 226 152 2109 1697 Fragments arranged by size: 2109 1697 961 580 226 191 152 88 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 3329 1870 Size: 73 1386 4545 Fragments arranged by size: 4545 1386 73 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3325 4780 618 Size: 325 217 627 1538 1455 1842 Fragments arranged by size: 1842 1538 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3323 1391 Size: 1932 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2546 2929 928 Size: 971 647 383 4003 Fragments arranged by size: 4003 971 647 383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BfrBI ATG'CAT Cuts at: 3107 3107 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4615 136 Size: 107 122 71 631 605 2943 1525 Fragments arranged by size: 2943 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 4065 5221 1790 Size: 2275 1156 2573 Fragments arranged by size: 2573 2275 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2633 3735 4693 Size: 416 53 83 399 24 1216 1102 958 Cuts at: 4693 442 Size: 1753 Fragments arranged by size: 1753 1216 1102 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 4664 1239 Size: 1106 2319 2579 Fragments arranged by size: 2579 2319 1106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4667 882 Size: 617 3168 2219 Fragments arranged by size: 3168 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2138 4437 4835 5109 5256 5667 2138 Size: 2299 398 274 147 411 2475 Fragments arranged by size: 2475 2299 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5779 Fragments arranged by size: 5779 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6004 BseYI C'CCAG_C Cuts at: 5207 5703 5207 Size: 496 5508 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5431 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4065 4150 5221 4065 Size: 85 1071 4848 Fragments arranged by size: 4848 1071 85 BsmI GAATG_Cn' Cuts at: 3107 3515 3608 3107 Size: 408 93 5503 Fragments arranged by size: 5503 408 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3323 4065 4150 5221 1391 Size: 399 1533 742 85 1071 2174 Fragments arranged by size: 2174 1533 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2452 3359 844 Size: 1608 907 3489 Fragments arranged by size: 3489 1608 907 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6004 BstBI TT'CG_AA Cuts at: 2725 4808 2725 Size: 2083 3921 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstYI r'GATC_y Cuts at: 1972 2652 3223 3334 3701 4086 4103 5555 5641 Size: 680 571 111 367 385 17 1452 86 Cuts at: 5641 5653 5999 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 680 571 385 367 346 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3288 3288 Size: 6004 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5452 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2334 2833 3520 4314 4334 2334 Size: 499 687 794 20 4004 Fragments arranged by size: 4004 794 687 499 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3000 3308 3000 Size: 308 5696 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969 Size: 54 1425 50 275 18 1621 889 1629 Cuts at: 5969 8 Size: 43 Fragments arranged by size: 1629 1621 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3934 1353 Size: 2581 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2693 2847 2923 4067 4664 5451 Size: 393 713 348 154 76 1144 597 787 Cuts at: 5451 5719 1239 Size: 268 1524 Fragments arranged by size: 1524 1144 787 713 597 393 348 268 154 76 EcoRI G'AATT_C Cuts at: 2454 3313 3691 2454 Size: 859 378 4767 Fragments arranged by size: 4767 859 378 EcoRV GAT'ATC Cuts at: 2490 2490 Size: 6004 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2953 2953 Size: 6004 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2415 2447 2770 2802 4645 Size: 32 135 32 843 32 323 32 1843 Cuts at: 4645 4677 4719 4751 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1843 843 323 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 2119 748 Size: 1371 4633 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2238 5118 5302 1305 Size: 933 2880 184 2007 Fragments arranged by size: 2880 2007 933 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2090 2590 3962 4321 4480 518 Size: 648 891 33 500 1372 359 159 2042 Fragments arranged by size: 2042 1372 891 648 500 359 159 33 MspA1I CmG'CkG Cuts at: 1606 3420 4097 5245 5490 1606 Size: 1814 677 1148 245 2120 Fragments arranged by size: 2120 1814 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5452 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NsiI A_TGCA'T Cuts at: 3109 3109 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2038 2818 3362 4907 1132 Size: 97 809 780 544 1545 2229 Fragments arranged by size: 2229 1545 809 780 544 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 2814 4903 2814 Size: 2089 3915 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PstI C_TGCA'G Cuts at: 830 3356 830 Size: 2526 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SfcI C'TryA_G Cuts at: 826 1045 2301 2808 3168 3352 4487 5168 5359 Size: 219 1256 507 360 184 1135 681 191 Cuts at: 5359 826 Size: 1471 Fragments arranged by size: 1471 1256 1135 681 507 360 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 2661 2722 3928 5 Size: 47 2609 61 1206 2081 Fragments arranged by size: 2609 2081 1206 61 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 3443 513 Size: 552 2378 3074 Fragments arranged by size: 3074 2378 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2260 2600 2647 Size: 274 80 33 51 1443 283 340 47 Cuts at: 2647 4250 5962 96 Size: 1603 1712 138 Fragments arranged by size: 1712 1603 1443 340 283 274 138 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5964 5996 5964 Size: 32 5972 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 4133 4133 Size: 6004 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI