FEM1A ORF clone pFN21AB3219

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB3219

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AB3219 Sequence (with vector) Restriction Map

Product ID

FHC01253

Flexi Clone PID

FXC01253

ORF Sequence

DNA sequence (2007 bp)
Protein sequence (669 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384759]

Gene

KIBB3219

Symbol

FEM1A

Alias : EPRAP

Description

fem-1 homolog a (C. elegans)

Original Clone ID

fj02440

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2007 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 669 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12135.1 7.1e-199 100.0 FEM1A CCDS4118.1 3.1e-72 64.8 FEM1C CCDS10228.1 4.3e-25 35.8 FEM1B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan NULL 41 56 PR01415 NULL NULL 563 577 PR01415 NULL HMMPfam IPR002110 41 70 PF00023 Ankyrin repeat IPR002110 83 113 PF00023 Ankyrin repeat IPR002110 149 179 PF00023 Ankyrin repeat IPR002110 534 578 PF00023 Ankyrin repeat IPR002110 580 611 PF00023 Ankyrin repeat HMMSmart IPR002110 2 33 SM00248 Ankyrin repeat IPR002110 40 70 SM00248 Ankyrin repeat IPR002110 82 111 SM00248 Ankyrin repeat IPR002110 115 145 SM00248 Ankyrin repeat IPR002110 149 178 SM00248 Ankyrin repeat IPR002110 182 211 SM00248 Ankyrin repeat IPR002110 214 243 SM00248 Ankyrin repeat IPR002110 534 576 SM00248 Ankyrin repeat IPR002110 580 609 SM00248 Ankyrin repeat ProfileScan IPR020683 2 236 PS50297 Ankyrin repeat-containing domain IPR002110 40 63 PS50088 Ankyrin repeat IPR002110 82 114 PS50088 Ankyrin repeat IPR002110 149 181 PS50088 Ankyrin repeat IPR002110 182 203 PS50088 Ankyrin repeat IPR002110 214 236 PS50088 Ankyrin repeat IPR002110 534 579 PS50088 Ankyrin repeat IPR020683 534 612 PS50297 Ankyrin repeat-containing domain IPR002110 580 612 PS50088 Ankyrin repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_061178 669 613538 100.0 100.0 XP_005272092 617 608767 64.8 100.0 NP_064562 617 608767 64.8 100.0 NP_056137 627 613539 35.8 99.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3219 Download

>KIBB3219  2007 bp
ATGGACCTCCGCACCGCCGTGTACAACGCCGCCCGTGATGGCAAGCTGCAGCTGCTCCAG
AAGCTGCTCAGCGGCCGGAGCCGGGAGGAACTGGACGAGCTGACGGGCGAGGTGGCCGGC
GGGGGAACGCCGCTACTCATCGCCGCCCGCTACGGCCACCTGGACGTGGTGGAGTACCTG
GTGGACCGGTGCGGCGCGAGCGTGGAGGCCGGTGGCTCGGTGCACTTCGATGGCGAGACC
ATCGAGGGCGCGCCGCCGCTGTGGGCCGCCTCCGCAGCCGGCCACCTGGACGTGGTGCGG
AGCCTGCTGCGCCGCGGGGCCTCGGTGAACCGCACCACGCGCACCAACTCCACGCCTCTC
CGCGCCGCCTGCTTCGACGGCCACCTGGAGGTGGTGCGCTACCTGGTCGGCGAGCACCAG
GCCGACCTGGAGGTGGCCAACCGGCACGGCCACACGTGCCTCATGATCTCGTGCTACAAG
GGCCACCGTGAGATCGCCCGCTACCTGCTGGAGCAGGGCGCCCAGGTGAACCGGCGCAGC
GCCAAGGGCAACACGGCCCTGCATGACTGCGCCGAGTCCGGCAGCCTGGAGATCCTGCAG
CTGCTGCTGGGGTGCAAGGCCCGCATGGAACGTGACGGCTACGGCATGACCCCGCTGCTC
GCGGCCAGCGTGACGGGCCACACCAACATCGTGGAGTACCTCATCCAGGAGCAGCCCGGC
CAGGAGCAGGTCGCAGGGGGAGAGGCTCAGCCTGGGCTGCCCCAAGAAGACCCCTCCACC
AGCCAGGGGTGTGCGCAGCCTCAGGGGGCTCCGTGCTGCAGCTCCTCCCCAGAGGAACCA
CTGAACGGGGAATCTTACGAAAGCTGCTGTCCCACCAGCCGGGAAGCTGCCGTGGAAGCC
TTGGAATTGCTGGGAGCTACGTATGTGGATAAGAAACGAGATCTGCTTGGGGCCCTTAAA
CACTGGAGGCGGGCCATGGAGCTGCGTCACCAGGGGGGCGAGTACCTGCCCAAACCGGAG
CCCCCACAGCTGGTCCTGGCCTATGACTATTCCAGGGAGGTCAACACCACCGAGGAGCTG
GAGGCGCTGATCACCGACCCGGATGAGATGCGCATGCAGGCCCTGTTGATCCGGGAGCGC
ATCCTCGGTCCCTCGCACCCGGACACTTCCTATTACATCCGTTACAGGGGTGCCGTGTAC
GCCGACTCGGGCAATTTCGAGCGCTGCATCCGCTTGTGGAAGTACGCCCTGGACATGCAA
CAGAGCAACCTGGAGCCTCTGAGCCCCATGACCGCCAGCAGCTTCCTCTCCTTCGCGGAA
CTCTTCTCCTACGTGCTTCAGGACCGGGCCGCCAAAGGCAGCCTGGGCACCCAGATCGGC
TTTGCAGACCTCATGGGGGTTCTCACCAAAGGGGTCCGGGAAGTGGAACGGGCCCTGCAG
CTGCCCAGGGAGCCCGGAGACTCAGCCCAGTTCACCAAGGCGCTGGCCATCATCCTCCAC
CTGCTCTACCTGCTGGAGAAAGTGGAGTGCACCCCCAGCCAGGAGCACCTGAAGCACCAG
ACCGTCTACCGCCTGCTCAAGTGCGCGCCCAGGGGCAAGAACGGCTTCACCCCTCTGCAC
ATGGCTGTGGACAAGGACACCACAAACGTGGGCCGCTATCCCGTGGGCAGATTCCCCTCC
CTGCACGTGGTCAAAGTGCTGCTCGACTGCGGGGCCGACCCGGACAGCAGGGATTTTGAC
AACAACACCCCGCTACACATAGCAGCCCAGAACAACTGCCCGGCCATCATGAATGCCCTG
ATCGAAGCAGGGGCCCACATGGACGCCACCAATGCCTTCAAGAAGACGGCCTACGAGCTG
CTGGACGAGAAGCTGCTGGCCAGGGGTACCATGCAGCCCTTCAACTACGTGACCCTGCAG
TGCCTTGCGGCCCGGGCCCTGGATAAGAACAAGATCCCTTACAAGGGCTTCATCCCGGAA
GATCTGGAGGCGTTCATCGAACTGCAC

ORF Protein

Cloned ORF protein sequence for pFN21AB3219 Download

>KIBB3219  669 aa
MDLRTAVYNAARDGKLQLLQKLLSGRSREELDELTGEVAGGGTPLLIAARYGHLDVVEYL
VDRCGASVEAGGSVHFDGETIEGAPPLWAASAAGHLDVVRSLLRRGASVNRTTRTNSTPL
RAACFDGHLEVVRYLVGEHQADLEVANRHGHTCLMISCYKGHREIARYLLEQGAQVNRRS
AKGNTALHDCAESGSLEILQLLLGCKARMERDGYGMTPLLAASVTGHTNIVEYLIQEQPG
QEQVAGGEAQPGLPQEDPSTSQGCAQPQGAPCCSSSPEEPLNGESYESCCPTSREAAVEA
LELLGATYVDKKRDLLGALKHWRRAMELRHQGGEYLPKPEPPQLVLAYDYSREVNTTEEL
EALITDPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQ
QSNLEPLSPMTASSFLSFAELFSYVLQDRAAKGSLGTQIGFADLMGVLTKGVREVERALQ
LPREPGDSAQFTKALAIILHLLYLLEKVECTPSQEHLKHQTVYRLLKCAPRGKNGFTPLH
MAVDKDTTNVGRYPVGRFPSLHVVKVLLDCGADPDSRDFDNNTPLHIAAQNNCPAIMNAL
IEAGAHMDATNAFKKTAYELLDEKLLARGTMQPFNYVTLQCLAARALDKNKIPYKGFIPE
DLEAFIELH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3219 Download

>pFN21AB3219  6712 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACCTCCGCACCGCCGTGTACAACGCCGCCCG
TGATGGCAAGCTGCAGCTGCTCCAGAAGCTGCTCAGCGGCCGGAGCCGGGAGGAACTGGA
CGAGCTGACGGGCGAGGTGGCCGGCGGGGGAACGCCGCTACTCATCGCCGCCCGCTACGG
CCACCTGGACGTGGTGGAGTACCTGGTGGACCGGTGCGGCGCGAGCGTGGAGGCCGGTGG
CTCGGTGCACTTCGATGGCGAGACCATCGAGGGCGCGCCGCCGCTGTGGGCCGCCTCCGC
AGCCGGCCACCTGGACGTGGTGCGGAGCCTGCTGCGCCGCGGGGCCTCGGTGAACCGCAC
CACGCGCACCAACTCCACGCCTCTCCGCGCCGCCTGCTTCGACGGCCACCTGGAGGTGGT
GCGCTACCTGGTCGGCGAGCACCAGGCCGACCTGGAGGTGGCCAACCGGCACGGCCACAC
GTGCCTCATGATCTCGTGCTACAAGGGCCACCGTGAGATCGCCCGCTACCTGCTGGAGCA
GGGCGCCCAGGTGAACCGGCGCAGCGCCAAGGGCAACACGGCCCTGCATGACTGCGCCGA
GTCCGGCAGCCTGGAGATCCTGCAGCTGCTGCTGGGGTGCAAGGCCCGCATGGAACGTGA
CGGCTACGGCATGACCCCGCTGCTCGCGGCCAGCGTGACGGGCCACACCAACATCGTGGA
GTACCTCATCCAGGAGCAGCCCGGCCAGGAGCAGGTCGCAGGGGGAGAGGCTCAGCCTGG
GCTGCCCCAAGAAGACCCCTCCACCAGCCAGGGGTGTGCGCAGCCTCAGGGGGCTCCGTG
CTGCAGCTCCTCCCCAGAGGAACCACTGAACGGGGAATCTTACGAAAGCTGCTGTCCCAC
CAGCCGGGAAGCTGCCGTGGAAGCCTTGGAATTGCTGGGAGCTACGTATGTGGATAAGAA
ACGAGATCTGCTTGGGGCCCTTAAACACTGGAGGCGGGCCATGGAGCTGCGTCACCAGGG
GGGCGAGTACCTGCCCAAACCGGAGCCCCCACAGCTGGTCCTGGCCTATGACTATTCCAG
GGAGGTCAACACCACCGAGGAGCTGGAGGCGCTGATCACCGACCCGGATGAGATGCGCAT
GCAGGCCCTGTTGATCCGGGAGCGCATCCTCGGTCCCTCGCACCCGGACACTTCCTATTA
CATCCGTTACAGGGGTGCCGTGTACGCCGACTCGGGCAATTTCGAGCGCTGCATCCGCTT
GTGGAAGTACGCCCTGGACATGCAACAGAGCAACCTGGAGCCTCTGAGCCCCATGACCGC
CAGCAGCTTCCTCTCCTTCGCGGAACTCTTCTCCTACGTGCTTCAGGACCGGGCCGCCAA
AGGCAGCCTGGGCACCCAGATCGGCTTTGCAGACCTCATGGGGGTTCTCACCAAAGGGGT
CCGGGAAGTGGAACGGGCCCTGCAGCTGCCCAGGGAGCCCGGAGACTCAGCCCAGTTCAC
CAAGGCGCTGGCCATCATCCTCCACCTGCTCTACCTGCTGGAGAAAGTGGAGTGCACCCC
CAGCCAGGAGCACCTGAAGCACCAGACCGTCTACCGCCTGCTCAAGTGCGCGCCCAGGGG
CAAGAACGGCTTCACCCCTCTGCACATGGCTGTGGACAAGGACACCACAAACGTGGGCCG
CTATCCCGTGGGCAGATTCCCCTCCCTGCACGTGGTCAAAGTGCTGCTCGACTGCGGGGC
CGACCCGGACAGCAGGGATTTTGACAACAACACCCCGCTACACATAGCAGCCCAGAACAA
CTGCCCGGCCATCATGAATGCCCTGATCGAAGCAGGGGCCCACATGGACGCCACCAATGC
CTTCAAGAAGACGGCCTACGAGCTGCTGGACGAGAAGCTGCTGGCCAGGGGTACCATGCA
GCCCTTCAACTACGTGACCCTGCAGTGCCTTGCGGCCCGGGCCCTGGATAAGAACAAGAT
CCCTTACAAGGGCTTCATCCCGGAAGATCTGGAGGCGTTCATCGAACTGCACGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT