(Circular) (Six-base) MAPSORT of: pFN21AB3219.seq Check: 5827 from: 1 to: 6712 pFN21AB3219 6712 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3513 3513 Size: 6712 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4522 278 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3570 4055 1937 Size: 1633 485 4594 Fragments arranged by size: 4594 1633 485 Acc65I G'GTAC_C Cuts at: 3890 4033 3890 Size: 143 6569 AclI AA'CG_TT Cuts at: 1563 4839 5212 1563 Size: 3276 373 3063 Fragments arranged by size: 3276 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3326 3575 4775 6159 6427 1632 Size: 1694 249 1200 1384 268 1917 Fragments arranged by size: 1917 1694 1384 1200 268 249 AfeI AGC'GCT Cuts at: 3227 3227 Size: 6712 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6515 AflIII A'CryG_T Cuts at: 1203 2457 5611 6588 1203 Size: 1254 3154 977 1327 Fragments arranged by size: 3154 1327 1254 977 AgeI A'CCGG_T Cuts at: 2190 5543 2190 Size: 3353 3359 AhdI GACnn_n'nnGTC Cuts at: 5441 5441 Size: 6712 AleI CACnn'nnGTG Cuts at: 1166 2021 3650 1166 Size: 855 1629 4228 Fragments arranged by size: 4228 1629 855 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3945 1184 Size: 2761 3951 AlwNI CAG_nnn'CTG Cuts at: 2068 2608 3127 4455 6027 2068 Size: 540 519 1328 1572 2753 Fragments arranged by size: 2753 1572 1328 540 519 ApaI G_GGCC'C Cuts at: 2959 3439 3820 3943 2959 Size: 480 381 123 5728 Fragments arranged by size: 5728 480 381 123 ApaLI G'TGCA_C Cuts at: 2225 3532 4769 5925 2225 Size: 1307 1237 1156 3012 Fragments arranged by size: 3012 1307 1237 1156 ApoI r'AATT_y Cuts at: 1493 4021 4247 4399 6508 1493 Size: 2528 226 152 2109 1697 Fragments arranged by size: 2528 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2253 2253 Size: 6712 AseI AT'TA_AT Cuts at: 160 1032 5266 160 Size: 872 4234 1606 Fragments arranged by size: 4234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 AvaI C'yCGr_G Cuts at: 1870 1943 3211 3936 4037 1870 Size: 73 1268 725 101 4545 Fragments arranged by size: 4545 1268 725 101 73 BamHI G'GATC_C Cuts at: 4042 4409 4042 Size: 367 6345 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2522 3194 3371 3890 4033 Size: 325 217 627 735 672 177 519 143 Cuts at: 4033 5488 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 735 672 627 519 325 217 177 143 BanII G_rGCy'C Cuts at: 1391 2815 2959 3027 3290 3439 3459 3820 3943 Size: 1424 144 68 263 149 20 361 123 Cuts at: 3943 4031 1391 Size: 88 4072 Fragments arranged by size: 4072 1424 361 263 149 144 123 88 68 20 BbeI G_GCGC'C Cuts at: 2526 2526 Size: 6712 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2778 3854 928 Size: 971 879 1076 3786 Fragments arranged by size: 3786 1076 971 879 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3361 3395 4903 4937 1903 Size: 34 1424 34 1508 34 3678 Fragments arranged by size: 3678 1508 1424 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4604 5814 4604 Size: 1210 5502 BclI T'GATC_A Cuts at: 1548 3093 1548 Size: 1545 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5323 136 Size: 107 122 71 631 605 3651 1525 Fragments arranged by size: 3651 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2944 3985 6707 2944 Size: 1041 2722 2949 Fragments arranged by size: 2949 2722 1041 BlpI GC'TnA_GC Cuts at: 2072 2751 4129 2072 Size: 679 1378 4655 Fragments arranged by size: 4655 1378 679 Bme1580I G_kGCm'C Cuts at: 1790 2229 2959 3374 3439 3536 3820 3943 4773 Size: 439 730 415 65 97 284 123 830 Cuts at: 4773 5929 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 830 730 439 415 284 123 97 65 BmgBI CAC'GTC Cuts at: 1318 2170 2296 1318 Size: 852 126 5734 Fragments arranged by size: 5734 852 126 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3466 4443 5401 Size: 416 53 83 399 24 2049 977 958 Cuts at: 5401 442 Size: 1753 Fragments arranged by size: 2049 1753 977 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2411 2453 2534 2612 2989 3104 3296 Size: 806 366 42 81 78 377 115 192 Cuts at: 3296 3539 4010 5372 1239 Size: 243 471 1362 2579 Fragments arranged by size: 2579 1362 806 471 377 366 243 192 115 81 78 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3566 4837 5702 6000 3566 Size: 1271 865 298 4278 Fragments arranged by size: 4278 1271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2234 5375 882 Size: 617 735 3141 2219 Fragments arranged by size: 3141 2219 735 617 BsaAI yAC'GTr Cuts at: 493 1204 2460 2925 3337 3691 3913 4503 493 Size: 711 1256 465 412 354 222 590 2702 Fragments arranged by size: 2702 1256 711 590 465 412 354 222 BsaBI GATnn'nnATC Cuts at: 6706 6706 Size: 6712 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2523 3828 4519 4901 Size: 53 83 186 936 990 1305 691 382 Cuts at: 4901 5536 275 Size: 635 1451 Fragments arranged by size: 1451 1305 990 936 691 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2190 3019 5145 5543 5817 5964 6375 2190 Size: 829 2126 398 274 147 411 2527 Fragments arranged by size: 2527 2126 829 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6487 Fragments arranged by size: 6487 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2818 3092 1186 Size: 1632 274 4806 Fragments arranged by size: 4806 1632 274 BseYI C'CCAG_C Cuts at: 2611 2914 3539 5915 6411 2611 Size: 303 625 2376 496 2912 Fragments arranged by size: 2912 2376 625 496 303 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3605 3671 3992 1163 Size: 573 1869 66 321 3883 Fragments arranged by size: 3883 1869 573 321 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2080 4162 4923 5951 1540 Size: 275 186 79 2082 761 1028 2301 Fragments arranged by size: 2301 2082 1028 761 275 186 79 BsiHKAI G_wGCw'C Cuts at: 2229 2421 3536 3552 4773 4858 5929 2229 Size: 192 1115 16 1221 85 1071 3012 Fragments arranged by size: 3012 1221 1115 1071 192 85 16 BsmI GAATG_Cn' Cuts at: 3802 4223 4316 3802 Size: 421 93 6198 Fragments arranged by size: 6198 421 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2421 2815 2959 3027 3290 3374 Size: 399 439 192 394 144 68 263 84 Cuts at: 3374 3439 3459 3536 3552 3820 3943 4031 4773 Size: 65 20 77 16 268 123 88 742 Cuts at: 4773 4858 5929 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 394 268 263 192 144 123 88 85 84 77 68 65 20 16 BspEI T'CCGG_A Cuts at: 6375 6375 Size: 6712 BspHI T'CATG_A Cuts at: 2466 3792 4601 6331 2466 Size: 1326 809 1730 2847 Fragments arranged by size: 2847 1730 1326 809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 2722 3018 3513 3522 4067 844 Size: 1673 205 296 495 9 545 3489 Fragments arranged by size: 3489 1673 545 495 296 205 9 BsrBI CCG'CTC Cuts at: 1394 1823 4599 1394 Size: 429 2776 3507 Fragments arranged by size: 3507 2776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5207 5381 65 Size: 1183 3959 174 1396 Fragments arranged by size: 3959 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 2190 2213 2282 2445 2535 5356 Size: 110 310 70 23 69 163 90 2821 Cuts at: 5356 5543 1700 Size: 187 2869 Fragments arranged by size: 2869 2821 310 187 163 110 90 70 69 23 BsrGI T'GTAC_A Cuts at: 96 2025 96 Size: 1929 4783 BssHII G'CGCG_C Cuts at: 1925 2253 3588 1925 Size: 328 1335 5049 Fragments arranged by size: 5049 1335 328 BssSI C'ACGA_G Cuts at: 2473 4772 5784 2473 Size: 2299 1012 3401 Fragments arranged by size: 3401 2299 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2608 794 Size: 1814 4898 BstBI TT'CG_AA Cuts at: 5516 5516 Size: 6712 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6712 BstXI CCAn_nnnn'nTGG Cuts at: 2695 3487 3652 2695 Size: 792 165 5755 Fragments arranged by size: 5755 792 165 BstYI r'GATC_y Cuts at: 1972 2595 2944 3957 3985 4042 4409 4794 4811 Size: 623 349 1013 28 57 367 385 17 Cuts at: 4811 6263 6349 6361 6707 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1013 623 385 367 349 346 86 57 28 17 12 Bsu36I CC'TnA_GG Cuts at: 2805 2805 Size: 6712 BtgI C'CryG_G Cuts at: 513 1065 2004 2317 2895 2979 3666 513 Size: 552 939 313 578 84 687 3559 Fragments arranged by size: 3559 939 687 578 552 313 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2128 487 Size: 683 56 902 5071 Fragments arranged by size: 5071 902 683 56 BtsI GCAGTG_nn' Cuts at: 3929 4228 5022 5042 3929 Size: 299 794 20 5599 Fragments arranged by size: 5599 794 299 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6677 DraI TTT'AAA Cuts at: 4016 4016 Size: 6712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5719 809 Size: 507 4403 1802 Fragments arranged by size: 4403 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2118 2158 Size: 54 1425 50 275 18 247 41 40 Cuts at: 2158 2284 2383 2439 2452 2667 2722 3489 3786 Size: 126 99 56 13 215 55 767 297 Cuts at: 3786 3882 4159 5048 6677 8 Size: 96 277 889 1629 43 Fragments arranged by size: 1629 1425 889 767 297 277 275 247 215 126 99 96 56 55 54 50 43 41 40 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2077 4159 1537 Size: 275 265 2082 4090 Fragments arranged by size: 4090 2082 275 265 EarI CTCTTCn'nnn_ Cuts at: 1353 3332 4642 1353 Size: 1979 1310 3423 Fragments arranged by size: 3423 1979 1310 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5299 5673 5819 387 Size: 1064 138 3710 374 146 1280 Fragments arranged by size: 3710 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2411 2453 2534 2612 2989 3104 Size: 393 413 366 42 81 78 377 115 Cuts at: 3104 3296 3326 3539 3575 4010 4775 5372 6159 Size: 192 30 213 36 435 765 597 787 Cuts at: 6159 6427 1239 Size: 268 1524 Fragments arranged by size: 1524 787 765 597 435 413 393 377 366 268 213 192 115 81 78 42 36 30 EcoNI CCTnn'n_nnAGG Cuts at: 2708 2708 Size: 6712 EcoO109I rG'GnC_Cy Cuts at: 2322 2955 2956 3124 3436 3816 3940 2322 Size: 633 1 168 312 380 124 5094 Fragments arranged by size: 5094 633 380 312 168 124 1 EcoRI G'AATT_C Cuts at: 4021 4399 4021 Size: 378 6334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3625 3625 Size: 6712 FspI TGC'GCA Cuts at: 2799 3116 5218 2799 Size: 317 2102 4293 Fragments arranged by size: 4293 2102 317 FspAI rTGC'GCAy Cuts at: 3116 3116 Size: 6712 HaeII r_GCGC'y Cuts at: 1678 2526 2547 3092 3229 3488 5859 1678 Size: 848 21 545 137 259 2371 2531 Fragments arranged by size: 2531 2371 848 545 259 137 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5353 5385 5427 5459 1373 Size: 32 135 32 3781 32 42 32 2626 Fragments arranged by size: 3781 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3067 4056 4302 669 Size: 988 281 1129 989 246 3079 Fragments arranged by size: 3079 1129 989 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6712 HpaI GTT'AAC Cuts at: 4302 4302 Size: 6712 KasI G'GCGC_C Cuts at: 2522 2522 Size: 6712 KpnI G_GTAC'C Cuts at: 3894 4037 3894 Size: 143 6569 MfeI C'AATT_G Cuts at: 4311 4311 Size: 6712 MluI A'CGCG_T Cuts at: 6588 6588 Size: 6712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5826 6010 1305 Size: 4521 184 2007 Fragments arranged by size: 4521 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 3491 3884 10 Size: 54 1425 343 609 1050 393 2838 Fragments arranged by size: 2838 1425 1050 609 393 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 2232 3629 3650 4670 5029 5188 Size: 648 855 211 1397 21 1020 359 159 Cuts at: 5188 518 Size: 2042 Fragments arranged by size: 2042 1397 1020 855 648 359 211 159 21 MspA1I CmG'CkG Cuts at: 1606 2056 2076 2263 2319 2605 2659 3034 3445 Size: 450 20 187 56 286 54 375 411 Cuts at: 3445 4128 4805 5953 6198 1606 Size: 683 677 1148 245 2120 Fragments arranged by size: 2120 1148 683 677 450 411 375 286 245 187 56 54 20 NaeI GCC'GGC Cuts at: 2122 2284 2122 Size: 162 6550 NarI GG'CG_CC Cuts at: 2523 2523 Size: 6712 NcoI C'CATG_G Cuts at: 513 1065 2004 2979 513 Size: 552 939 975 4246 Fragments arranged by size: 4246 975 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6712 NgoMIV G'CCGG_C Cuts at: 2120 2282 2120 Size: 162 6550 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6712 NotI GC'GGCC_GC Cuts at: 4159 4159 Size: 6712 NspI r_CATG'y Cuts at: 1132 1229 3121 3262 4070 5615 1132 Size: 97 1892 141 808 1545 2229 Fragments arranged by size: 2229 1892 1545 808 141 97 PasI CC'CwG_GG Cuts at: 1348 3450 3594 1348 Size: 2102 144 4466 Fragments arranged by size: 4466 2102 144 PciI A'CATG_T Cuts at: 5611 5611 Size: 6712 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6712 PfoI T'CCnGG_A Cuts at: 2709 3135 3420 3978 2709 Size: 426 285 558 5443 Fragments arranged by size: 5443 558 426 285 PmeI GTTT'AAAC Cuts at: 4016 4016 Size: 6712 PmlI CAC'GTG Cuts at: 2460 3691 2460 Size: 1231 5481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4781 4813 6294 6326 4781 Size: 32 1481 32 5167 Fragments arranged by size: 5167 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6712 PsiI TTA'TAA Cuts at: 4282 4282 Size: 6712 PspOMI G'GGCC_C Cuts at: 2955 3435 3816 3939 2955 Size: 480 381 123 5728 Fragments arranged by size: 5728 480 381 123 PstI C_TGCA'G Cuts at: 830 2055 2604 2825 3444 3924 4064 830 Size: 1225 549 221 619 480 140 3478 Fragments arranged by size: 3478 1225 619 549 480 221 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6712 PvuII CAG'CTG Cuts at: 2056 2605 3034 3445 2056 Size: 549 429 411 5323 Fragments arranged by size: 5323 549 429 411 SacII CC_GC'GG Cuts at: 2320 2320 Size: 6712 SalI G'TCGA_C Cuts at: 1936 4054 1936 Size: 2118 4594 SbfI CC_TGCA'GG Cuts at: 4064 4064 Size: 6712 ScaI AGT'ACT Cuts at: 4960 6672 4960 Size: 1712 5000 SexAI A'CCwGG_T Cuts at: 2181 2406 2181 Size: 225 6487 SfcI C'TryA_G Cuts at: 826 1045 2051 2600 2821 3440 3920 4060 5195 Size: 219 1006 549 221 619 480 140 1135 Cuts at: 5195 5876 6067 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 619 549 480 221 219 191 140 SfoI GGC'GCC Cuts at: 2524 2524 Size: 6712 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6712 SmaI CCC'GGG Cuts at: 1872 3938 4039 1872 Size: 2066 101 4545 Fragments arranged by size: 4545 2066 101 SmlI C'TyrA_G Cuts at: 820 1017 1943 3581 4816 5717 5979 820 Size: 197 926 1638 1235 901 262 1553 Fragments arranged by size: 1638 1553 1235 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2925 4503 493 Size: 2432 1578 2702 Fragments arranged by size: 2702 2432 1578 SpeI A'CTAG_T Cuts at: 152 152 Size: 6712 SphI G_CATG'C Cuts at: 1132 3121 4070 1132 Size: 1989 949 3774 Fragments arranged by size: 3774 1989 949 SrfI GCCC'GGGC Cuts at: 3938 3938 Size: 6712 SspI AAT'ATT Cuts at: 5 52 4636 5 Size: 47 4584 2081 Fragments arranged by size: 4584 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6712 StyI C'CwwG_G Cuts at: 513 1065 2004 2547 2904 2979 3480 4151 513 Size: 552 939 543 357 75 501 671 3074 Fragments arranged by size: 3074 939 671 552 543 501 357 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3140 3389 4739 4756 4909 5094 5539 Size: 1255 1560 249 1350 17 153 185 445 Cuts at: 5539 325 Size: 1498 Fragments arranged by size: 1560 1498 1350 1255 445 249 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 4958 6670 Size: 274 80 33 51 1443 48 2933 1712 Cuts at: 6670 96 Size: 138 Fragments arranged by size: 2933 1712 1443 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3021 3053 6672 6704 3021 Size: 32 3619 32 3029 Fragments arranged by size: 3619 3029 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6712 XbaI T'CTAG_A Cuts at: 4048 4048 Size: 6712 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3079 3364 3079 Size: 285 6427 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6712 XmaI C'CCGG_G Cuts at: 1870 3936 4037 1870 Size: 2066 101 4545 Fragments arranged by size: 4545 2066 101 XmnI GAAnn'nnTTC Cuts at: 3608 4841 3608 Size: 1233 5479 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4520 276 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SwaI