GRAMD1A ORF clone pFN21AA1533

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Halotag ORF Clone pFN21AA1533

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Clone info.

Species

human

Clone Name

pFN21AA1533 Sequence (with vector) Restriction Map

Product ID

FHC00245

Flexi Clone PID

FXC00245

Original Clone PID

ORK00245

ORF Sequence

DNA sequence (2175 bp)
Protein sequence (725 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384616]

Gene

KIAA1533

Symbol

GRAMD1A

Alias : KIAA1533

Description

GRAM domain containing 1A

Original Clone ID

ee00796

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2175 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 725 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS42546.1 3.7e-212 99.2 GRAMD1A CCDS46046.1 1.8e-209 98.7 GRAMD1A CCDS53720.1 8.6e-78 46.7 GRAMD1B CCDS66253.1 7.9e-75 46.4 GRAMD1B CCDS81640.1 1.9e-74 47.2 GRAMD1B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004182 96 163 PF02893 GRAM HMMSmart IPR004182 96 163 SM00568 GRAM
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 609 IPSALVLISIVLIILIALNVLLF 631 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1533 Download

>KIAA1533  2175 bp
ATGGCATATGTTAAGGCTTGGAGCACCACACCCCACTCTGGCCGGAGCACGCCAAGCAGC
TCCCCATCGCTCCGGAAACGGCTGCAGCTCCTGCCCCCAAGCCGGCCCCCACCTGAGCCA
GAACCAGGCACCATGGTGGAGAAGGGATCAGATAGCTCCTCAGAGAAGGGTGGGGTGCCT
GGGACCCCCAGCACCCAGAGCCTAGGCAGCCGGAACTTCATCCGCAACAGCAAGAAGATG
CAGAGCTGGTACAGTATGCTGAGCCCCACTTATAAGCAGCGTAATGAGGACTTCCGGAAA
CTGTTCAGCAAACTCCCCGAAGCAGAACGCCTCATTGTGGATTACTCCTGCGCCCTGCAG
CGTGAGATCCTGCTCCAGGGCCGCCTCTACCTCTCTGAGAACTGGATCTGCTTCTACAGC
AACATCTTCCGCTGGGAGACCACGATCTCCATCCAGCTGAAGGAAGTGACATGTCTGAAG
AAGGAAAAGACGGCCAAGCTGATCCCCAACGCCATCCAGATCTGCACGGAGAGCGAGAAG
CATTTCTTCACTTCCTTTGGGGCCCGTGACCGCTGCTTCCTCCTCATCTTCCGCCTCTGG
CAGAATGCACTGCTTGAAAAGACGCTGAGTCCCCGCGAGCTCTGGCACCTGGTGCATCAG
TGCTACGGCTCAGAGCTGGGCCTCACCAGTGAGGATGAGGACTATGTCTCCCCCTTGCAG
CTGAACGGTCTGGGGACCCCCAAGGAAGTGGGAGATGTGATCGCCCTGAGCGACATCACC
TCCTCGGGGGCAGCTGACCGCAGCCAGGAGCCAAGCCCAGTGGGTTCGCGCCGTGGCCAT
GTCACGCCCAACCTTTCCCGAGCCAGCAGCGACGCAGACCATGGGGCAGAGGAGGACAAG
GAGGAGCAGGTAGACAGCCAGCCAGACGCCTCCTCCAGCCAGACAGTGACCCCGGTGGCT
GAACCCCCGAGCACAGAGCCCACCCAGCCTGACGGGCCCACCACCCTGGGCCCCTTGGAT
CTGCTGCCCAGTGAGGAGCTATTGACAGACACAAGTAACTCCTCTTCATCCACTGGGGAG
GAAGCGGACTTGGCTGCCCTGCTTCCCGACCTCTCCGGCCGCCTCCTCATCAACTCTGTC
TTCCATGTGGGCGCTGAGCGGCTCCAGCAGATGCTCTTCTCGGACTCGCCCTTCCTCCAG
GGCTTCCTACAGCAGTGCAAGTTCACAGACGTGACGCTGAGCCCCTGGAGTGGGGACAGC
AAGTGCCACCAGCGCCGGGTGCTGACGTACACCATCCCCATCAGCAACCCACTGGGCCCC
AAGAGCGCCTCCGTGGTGGAGACACAGACGCTGTTCCGGCGCGGCCCCCAGGCCGGCGGG
TGTGTGGTGGACTCCGAGGTGCTGACGCAGGGCATCCCCTACCAGGACTACTTCTACACT
GCCCACCGCTACTGCATCCTGGGTCTGGCCCGGAACAAGGCGCGGCTCCGAGTGTCTTCT
GAGATCCGCTACCGAAAGCAGCCGTGGAGCCTGGTGAAGTCGCTCATTGAGAAGAACTCG
TGGAGCGGCATTGAAGACTATTTCCACCATCTGGAGCGAGAGCTCGCCAAGGCTGAGAAG
CTGTCTCTGGAGGAAGGCGGGAAGGATGCCCGGGGCTTGCTATCCGGCCTGCGGCGGCGG
AAGCGGCCCCTGAGCTGGCGGGCTCACGGGGACGGGCCCCAGCACCCAGATCCTGACCCC
TGTGCCCGGGCCGGCATTCACACCTCGGGCTCCCTCAGCTCCCGCTTCTCCGAACCATCT
GTGGACCAGGGCCCCGGGGCAGGCATCCCCAGTGCCCTGGTTCTCATCAGCATTGTCCTT
ATCATCCTCATCGCCCTCAACGTCCTGCTCTTCTACCGCCTCTGGTCCCTGGAAAGGACA
GCCCACACCTTTGAGTCCTGGCACAGCCTGGCCCTGGCCAAGGGCAAGTTCCCCCAGACG
GCCACAGAGTGGGCCGAGATCCTGGCGCTGCAGAAGCAATTCCACAGCGTGGAGGTGCAC
AAGTGGAGGCAGATCCTGCGGGCCTCCGTGGAGCTCCTGGATGAGATGAAGTTCTCGCTG
GAGAAGCTGCACCAAGGCATCACAGTCTCAGACCCTCCCTTTGACACCCAGCCCCGGCCC
GATGACAGCTTTTCC

ORF Protein

Cloned ORF protein sequence for pFN21AA1533 Download

>KIAA1533  725 aa
MAYVKAWSTTPHSGRSTPSSSPSLRKRLQLLPPSRPPPEPEPGTMVEKGSDSSSEKGGVP
GTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQ
REILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEK
HFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVSPLQ
LNGLGTPKEVGDVIALSDITSSGAADRSQEPSPVGSRRGHVTPNLSRASSDADHGAEEDK
EEQVDSQPDASSSQTVTPVAEPPSTEPTQPDGPTTLGPLDLLPSEELLTDTSNSSSSTGE
EADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQGFLQQCKFTDVTLSPWSGDS
KCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYT
AHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEK
LSLEEGGKDARGLLSGLRRRKRPLSWRAHGDGPQHPDPDPCARAGIHTSGSLSSRFSEPS
VDQGPGAGIPSALVLISIVLIILIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQT
ATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDTQPRP
DDSFS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1533 Download

>pFN21AA1533  6880 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCATATGTTAAGGCTTGGAGCACCACACCCCA
CTCTGGCCGGAGCACGCCAAGCAGCTCCCCATCGCTCCGGAAACGGCTGCAGCTCCTGCC
CCCAAGCCGGCCCCCACCTGAGCCAGAACCAGGCACCATGGTGGAGAAGGGATCAGATAG
CTCCTCAGAGAAGGGTGGGGTGCCTGGGACCCCCAGCACCCAGAGCCTAGGCAGCCGGAA
CTTCATCCGCAACAGCAAGAAGATGCAGAGCTGGTACAGTATGCTGAGCCCCACTTATAA
GCAGCGTAATGAGGACTTCCGGAAACTGTTCAGCAAACTCCCCGAAGCAGAACGCCTCAT
TGTGGATTACTCCTGCGCCCTGCAGCGTGAGATCCTGCTCCAGGGCCGCCTCTACCTCTC
TGAGAACTGGATCTGCTTCTACAGCAACATCTTCCGCTGGGAGACCACGATCTCCATCCA
GCTGAAGGAAGTGACATGTCTGAAGAAGGAAAAGACGGCCAAGCTGATCCCCAACGCCAT
CCAGATCTGCACGGAGAGCGAGAAGCATTTCTTCACTTCCTTTGGGGCCCGTGACCGCTG
CTTCCTCCTCATCTTCCGCCTCTGGCAGAATGCACTGCTTGAAAAGACGCTGAGTCCCCG
CGAGCTCTGGCACCTGGTGCATCAGTGCTACGGCTCAGAGCTGGGCCTCACCAGTGAGGA
TGAGGACTATGTCTCCCCCTTGCAGCTGAACGGTCTGGGGACCCCCAAGGAAGTGGGAGA
TGTGATCGCCCTGAGCGACATCACCTCCTCGGGGGCAGCTGACCGCAGCCAGGAGCCAAG
CCCAGTGGGTTCGCGCCGTGGCCATGTCACGCCCAACCTTTCCCGAGCCAGCAGCGACGC
AGACCATGGGGCAGAGGAGGACAAGGAGGAGCAGGTAGACAGCCAGCCAGACGCCTCCTC
CAGCCAGACAGTGACCCCGGTGGCTGAACCCCCGAGCACAGAGCCCACCCAGCCTGACGG
GCCCACCACCCTGGGCCCCTTGGATCTGCTGCCCAGTGAGGAGCTATTGACAGACACAAG
TAACTCCTCTTCATCCACTGGGGAGGAAGCGGACTTGGCTGCCCTGCTTCCCGACCTCTC
CGGCCGCCTCCTCATCAACTCTGTCTTCCATGTGGGCGCTGAGCGGCTCCAGCAGATGCT
CTTCTCGGACTCGCCCTTCCTCCAGGGCTTCCTACAGCAGTGCAAGTTCACAGACGTGAC
GCTGAGCCCCTGGAGTGGGGACAGCAAGTGCCACCAGCGCCGGGTGCTGACGTACACCAT
CCCCATCAGCAACCCACTGGGCCCCAAGAGCGCCTCCGTGGTGGAGACACAGACGCTGTT
CCGGCGCGGCCCCCAGGCCGGCGGGTGTGTGGTGGACTCCGAGGTGCTGACGCAGGGCAT
CCCCTACCAGGACTACTTCTACACTGCCCACCGCTACTGCATCCTGGGTCTGGCCCGGAA
CAAGGCGCGGCTCCGAGTGTCTTCTGAGATCCGCTACCGAAAGCAGCCGTGGAGCCTGGT
GAAGTCGCTCATTGAGAAGAACTCGTGGAGCGGCATTGAAGACTATTTCCACCATCTGGA
GCGAGAGCTCGCCAAGGCTGAGAAGCTGTCTCTGGAGGAAGGCGGGAAGGATGCCCGGGG
CTTGCTATCCGGCCTGCGGCGGCGGAAGCGGCCCCTGAGCTGGCGGGCTCACGGGGACGG
GCCCCAGCACCCAGATCCTGACCCCTGTGCCCGGGCCGGCATTCACACCTCGGGCTCCCT
CAGCTCCCGCTTCTCCGAACCATCTGTGGACCAGGGCCCCGGGGCAGGCATCCCCAGTGC
CCTGGTTCTCATCAGCATTGTCCTTATCATCCTCATCGCCCTCAACGTCCTGCTCTTCTA
CCGCCTCTGGTCCCTGGAAAGGACAGCCCACACCTTTGAGTCCTGGCACAGCCTGGCCCT
GGCCAAGGGCAAGTTCCCCCAGACGGCCACAGAGTGGGCCGAGATCCTGGCGCTGCAGAA
GCAATTCCACAGCGTGGAGGTGCACAAGTGGAGGCAGATCCTGCGGGCCTCCGTGGAGCT
CCTGGATGAGATGAAGTTCTCGCTGGAGAAGCTGCACCAAGGCATCACAGTCTCAGACCC
TCCCTTTGACACCCAGCCCCGGCCCGATGACAGCTTTTCCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT