(Circular) (Six-base) MAPSORT of: pFN21AA1533.seq Check: 1712 from: 1 to: 6880 pFN21AA1533 6880 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4690 278 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2916 4223 1937 Size: 979 1307 4594 Fragments arranged by size: 4594 1307 979 Acc65I G'GTAC_C Cuts at: 4201 4201 Size: 6880 AclI AA'CG_TT Cuts at: 1563 5007 5380 1563 Size: 3444 373 3063 Fragments arranged by size: 3444 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2483 2501 4943 6327 6595 1632 Size: 851 18 2442 1384 268 1917 Fragments arranged by size: 2442 1917 1384 851 268 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6683 AflIII A'CryG_T Cuts at: 1203 2474 5779 6756 1203 Size: 1271 3305 977 1327 Fragments arranged by size: 3305 1327 1271 977 AgeI A'CCGG_T Cuts at: 5711 5711 Size: 6880 AhdI GACnn_n'nnGTC Cuts at: 2631 5609 2631 Size: 2978 3902 AleI CACnn'nnGTG Cuts at: 1166 2138 3233 1166 Size: 972 1095 4813 Fragments arranged by size: 4813 1095 972 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2258 1184 Size: 1074 5806 AlwNI CAG_nnn'CTG Cuts at: 2095 3355 3737 4060 4623 6195 2095 Size: 1260 382 323 563 1572 2780 Fragments arranged by size: 2780 1572 1260 563 382 323 ApaI G_GGCC'C Cuts at: 2569 3003 3017 3323 3723 3818 2569 Size: 434 14 306 400 95 5631 Fragments arranged by size: 5631 434 400 306 95 14 ApaLI G'TGCA_C Cuts at: 4040 4937 6093 4040 Size: 897 1156 4827 Fragments arranged by size: 4827 1156 897 ApoI r'AATT_y Cuts at: 1493 4189 4415 4567 6676 1493 Size: 2696 226 152 2109 1697 Fragments arranged by size: 2696 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5434 160 Size: 872 4402 1606 Fragments arranged by size: 4402 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 AvaI C'yCGr_G Cuts at: 1870 1943 2788 2862 2971 3654 3750 3769 3818 Size: 73 845 74 109 683 96 19 49 Cuts at: 3818 4205 1870 Size: 387 4545 Fragments arranged by size: 4545 845 683 387 109 96 74 73 49 19 AvrII C'CTAG_G Cuts at: 2206 2206 Size: 6880 BamHI G'GATC_C Cuts at: 4210 4577 4210 Size: 367 6513 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2179 2649 4201 5656 Size: 325 217 627 345 47 470 1552 1455 Cuts at: 5656 618 Size: 1842 Fragments arranged by size: 1842 1552 1455 627 470 345 325 217 47 BanII G_rGCy'C Cuts at: 1391 2270 2569 2646 2985 3003 3017 3248 3323 Size: 879 299 77 339 18 14 231 75 Cuts at: 3323 3609 3709 3723 3776 3818 4080 4199 1391 Size: 286 100 14 53 42 262 119 4072 Fragments arranged by size: 4072 879 339 299 286 262 231 119 100 77 75 53 42 18 14 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3136 3492 3585 928 Size: 971 1237 356 93 4223 Fragments arranged by size: 4223 1237 971 356 93 BbvCI CC'TCA_GC Cuts at: 3779 3779 Size: 6880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5071 5105 1903 Size: 34 3134 34 3678 Fragments arranged by size: 3678 3134 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4772 5982 4772 Size: 1210 5670 BclI T'GATC_A Cuts at: 1548 1548 Size: 6880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2998 5491 136 Size: 107 122 71 631 605 1326 2493 1525 Fragments arranged by size: 2493 1525 1326 631 605 122 107 71 BglII A'GATC_T Cuts at: 2523 6875 2523 Size: 4352 2528 BlpI GC'TnA_GC Cuts at: 2264 3159 3242 4297 2264 Size: 895 83 1055 4847 Fragments arranged by size: 4847 1055 895 83 Bme1580I G_kGCm'C Cuts at: 1790 2569 3003 3017 3323 3723 3751 3818 3841 Size: 779 434 14 306 400 28 67 23 Cuts at: 3841 4044 4941 6097 1790 Size: 203 897 1156 2573 Fragments arranged by size: 2573 1156 897 779 434 400 306 203 67 28 23 14 BmgBI CAC'GTC Cuts at: 1318 3235 1318 Size: 1917 4963 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2816 3027 3087 Size: 416 53 83 399 24 1399 211 60 Cuts at: 3087 3326 3828 4611 5569 442 Size: 239 502 783 958 1753 Fragments arranged by size: 1753 1399 958 783 502 416 399 239 211 83 60 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6880 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3577 3577 Size: 6880 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2923 3152 3185 3271 3617 3653 4124 Size: 1124 560 229 33 86 346 36 471 Cuts at: 4124 5540 1239 Size: 1416 2579 Fragments arranged by size: 2579 1416 1124 560 471 346 229 86 36 33 Bpu10I CC'TnA_GC Cuts at: 2118 2771 3695 3779 2118 Size: 653 924 84 5219 Fragments arranged by size: 5219 924 653 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5005 5870 6168 5005 Size: 865 298 5717 Fragments arranged by size: 5717 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2435 5543 882 Size: 617 936 3108 2219 Fragments arranged by size: 3108 2219 936 617 BsaAI yAC'GTr Cuts at: 493 1204 4671 493 Size: 711 3467 2702 Fragments arranged by size: 3467 2702 711 BsaBI GATnn'nnATC Cuts at: 2453 6874 2453 Size: 4421 2459 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2931 4687 5069 5704 Size: 53 83 186 936 1398 1756 382 635 Cuts at: 5704 275 Size: 1451 Fragments arranged by size: 1756 1451 1398 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2076 2298 5313 5711 5985 6132 6543 2076 Size: 222 3015 398 274 147 411 2413 Fragments arranged by size: 3015 2413 411 398 274 222 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2726 2768 2798 2921 Size: 30 165 30 1293 30 42 30 123 Cuts at: 2921 2951 1178 Size: 30 5107 Fragments arranged by size: 5107 1293 165 123 42 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2152 2576 2776 2909 2921 2926 3053 3055 Size: 966 424 200 133 12 5 127 2 Cuts at: 3055 3119 1186 Size: 64 4947 Fragments arranged by size: 4947 966 424 200 133 127 64 12 5 2 BseYI C'CCAG_C Cuts at: 2192 2436 2680 2988 3723 4152 6083 6579 2192 Size: 244 244 308 735 429 1931 496 2493 Fragments arranged by size: 2493 1931 735 496 429 308 244 244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2512 4097 1163 Size: 573 776 1585 3946 Fragments arranged by size: 3946 1585 776 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 4330 5091 6119 1540 Size: 275 186 1123 1206 761 1028 2301 Fragments arranged by size: 2301 1206 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 2054 2646 2978 3609 4044 4080 4941 5026 Size: 24 592 332 631 435 36 861 85 Cuts at: 5026 6097 2030 Size: 1071 2813 Fragments arranged by size: 2813 1071 861 631 592 435 332 85 36 24 BsmI GAATG_Cn' Cuts at: 2614 3759 4391 4484 2614 Size: 1145 632 93 5010 Fragments arranged by size: 5010 1145 632 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2054 2270 2569 2646 2978 2985 Size: 399 240 24 216 299 77 332 7 Cuts at: 2985 3003 3017 3248 3323 3609 3709 3723 3751 Size: 18 14 231 75 286 100 14 28 Cuts at: 3751 3776 3818 3841 4044 4080 4199 4941 5026 Size: 25 42 23 203 36 119 742 85 Cuts at: 5026 6097 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 332 299 286 240 231 216 203 119 100 85 77 75 42 36 28 25 24 23 18 14 14 7 BspEI T'CCGG_A Cuts at: 2076 2298 6543 2076 Size: 222 4245 2413 Fragments arranged by size: 4245 2413 222 BspHI T'CATG_A Cuts at: 4769 6499 4769 Size: 1730 5150 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2902 4235 844 Size: 2058 1333 3489 Fragments arranged by size: 3489 2058 1333 BsrBI CCG'CTC Cuts at: 1394 1823 3163 3570 4767 1394 Size: 429 1340 407 1197 3507 Fragments arranged by size: 3507 1340 1197 429 407 BsrDI GCAATG_nn' Cuts at: 65 1248 5375 5549 65 Size: 1183 4127 174 1396 Fragments arranged by size: 4127 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2106 3377 3755 5524 5711 1700 Size: 110 296 1271 378 1769 187 2869 Fragments arranged by size: 2869 1769 1271 378 296 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6880 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6880 BssSI C'ACGA_G Cuts at: 3562 4940 5952 3562 Size: 1378 1012 4490 Fragments arranged by size: 4490 1378 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2095 2656 4060 794 Size: 1301 561 1404 3614 Fragments arranged by size: 3614 1404 1301 561 BstBI TT'CG_AA Cuts at: 5684 5684 Size: 6880 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6880 BstXI CCAn_nnnn'nTGG Cuts at: 2752 3010 3841 2752 Size: 258 831 5791 Fragments arranged by size: 5791 831 258 BstYI r'GATC_y Cuts at: 1972 2370 2409 2523 3022 3507 3733 4002 4056 Size: 398 39 114 499 485 226 269 54 Cuts at: 4056 4210 4577 4962 4979 6431 6517 6529 6875 Size: 154 367 385 17 1452 86 12 346 Cuts at: 6875 1972 Size: 1977 Fragments arranged by size: 1977 1452 499 485 398 385 367 346 269 226 154 114 86 54 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2836 2884 3336 3527 4071 Size: 552 939 132 700 48 452 191 544 Cuts at: 4071 513 Size: 3322 Fragments arranged by size: 3322 939 700 552 544 452 191 132 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2055 3859 487 Size: 683 56 829 1804 3508 Fragments arranged by size: 3508 1804 829 683 56 BtsI GCAGTG_nn' Cuts at: 2612 3224 3441 4396 5190 5210 2612 Size: 612 217 955 794 20 4282 Fragments arranged by size: 4282 955 794 612 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6880 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2805 2840 529 Size: 35 2241 35 4569 Fragments arranged by size: 4569 2241 35 35 DraI TTT'AAA Cuts at: 4184 4184 Size: 6880 DraIII CAC_nnn'GTG Cuts at: 2656 3993 4033 2656 Size: 1337 40 5503 Fragments arranged by size: 5503 1337 40 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5887 809 Size: 507 4571 1802 Fragments arranged by size: 4571 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2496 2839 Size: 54 1425 50 275 18 214 452 343 Cuts at: 2839 3121 3960 3984 4327 5216 6845 8 Size: 282 839 24 343 889 1629 43 Fragments arranged by size: 1629 1425 889 839 452 343 343 282 275 214 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 4327 1537 Size: 275 1309 1206 4090 Fragments arranged by size: 4090 1309 1206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3073 3185 3899 4810 1353 Size: 1720 112 714 911 3423 Fragments arranged by size: 3423 1720 911 714 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2585 3697 5467 5841 5987 387 Size: 1064 138 996 1112 1770 374 146 1280 Fragments arranged by size: 1770 1280 1112 1064 996 374 146 138 EcoICRI GAG'CTC Cuts at: 2644 3607 4078 2644 Size: 963 471 5446 Fragments arranged by size: 5446 963 471 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2483 2501 2923 3152 3185 3271 Size: 393 731 120 18 422 229 33 86 Cuts at: 3271 3617 3653 4124 4943 5540 6327 6595 1239 Size: 346 36 471 819 597 787 268 1524 Fragments arranged by size: 1524 819 787 731 597 471 422 393 346 268 229 120 86 36 33 18 EcoNI CCTnn'n_nnAGG Cuts at: 2378 3962 2378 Size: 1584 5296 EcoO109I rG'GnC_Cy Cuts at: 2187 2565 2739 3014 3320 3720 3814 3815 2187 Size: 378 174 275 306 400 94 1 5252 Fragments arranged by size: 5252 400 378 306 275 174 94 1 EcoRI G'AATT_C Cuts at: 4189 4567 4189 Size: 378 6502 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 6880 FspI TGC'GCA Cuts at: 5386 5386 Size: 6880 HaeII r_GCGC'y Cuts at: 1678 3159 3280 3333 4013 6027 1678 Size: 1481 121 53 680 2014 2531 Fragments arranged by size: 2531 2014 1481 680 121 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2147 2167 2179 2617 Size: 32 135 32 563 12 20 12 438 Cuts at: 2617 2649 2696 2728 2748 2768 2780 2800 2921 Size: 32 47 32 20 20 12 20 121 Cuts at: 2921 2953 3611 3643 5521 5553 5595 5627 1373 Size: 32 658 32 1878 32 42 32 2626 Fragments arranged by size: 2626 1878 658 563 438 135 121 47 42 32 32 32 32 32 32 32 32 20 20 20 20 12 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4224 4470 669 Size: 988 281 2286 246 3079 Fragments arranged by size: 3079 2286 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6880 HpaI GTT'AAC Cuts at: 4470 4470 Size: 6880 KpnI G_GTAC'C Cuts at: 4205 4205 Size: 6880 MfeI C'AATT_G Cuts at: 4479 4479 Size: 6880 MluI A'CGCG_T Cuts at: 6756 6756 Size: 6880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5994 6178 1305 Size: 4689 184 2007 Fragments arranged by size: 4689 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2841 3962 10 Size: 54 1425 343 1009 1121 2928 Fragments arranged by size: 2928 1425 1121 1009 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 3233 4838 5197 5356 518 Size: 648 972 1095 1605 359 159 2042 Fragments arranged by size: 2042 1605 1095 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2436 2461 2577 2725 2798 4296 4973 6121 Size: 830 25 116 148 73 1498 677 1148 Cuts at: 6121 6366 1606 Size: 245 2120 Fragments arranged by size: 2120 1498 1148 830 677 245 148 116 73 25 NaeI GCC'GGC Cuts at: 2108 3379 3757 2108 Size: 1271 378 5231 Fragments arranged by size: 5231 1271 378 NcoI C'CATG_G Cuts at: 513 1065 2004 2136 2884 513 Size: 552 939 132 748 4509 Fragments arranged by size: 4509 939 748 552 132 NdeI CA'TA_TG Cuts at: 387 2011 387 Size: 1624 5256 NgoMIV G'CCGG_C Cuts at: 2106 3377 3755 2106 Size: 1271 378 5231 Fragments arranged by size: 5231 1271 378 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6880 NotI GC'GGCC_GC Cuts at: 4327 4327 Size: 6880 NspI r_CATG'y Cuts at: 1132 1229 2478 4238 5783 1132 Size: 97 1249 1760 1545 2229 Fragments arranged by size: 2229 1760 1545 1249 97 PasI CC'CwG_GG Cuts at: 1348 3010 1348 Size: 1662 5218 PciI A'CATG_T Cuts at: 2474 5779 2474 Size: 3305 3575 PflMI CCAn_nnn'nTGG Cuts at: 1108 3595 3993 4033 1108 Size: 2487 398 40 3955 Fragments arranged by size: 3955 2487 398 40 PfoI T'CCnGG_A Cuts at: 4080 4080 Size: 6880 PmeI GTTT'AAAC Cuts at: 4184 4184 Size: 6880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 4949 4981 6462 6494 4079 Size: 32 838 32 1481 32 4465 Fragments arranged by size: 4465 1481 838 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2187 2739 2187 Size: 552 6328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6880 PsiI TTA'TAA Cuts at: 2277 4450 2277 Size: 2173 4707 PspOMI G'GGCC_C Cuts at: 2565 2999 3013 3319 3719 3814 2565 Size: 434 14 306 400 95 5631 Fragments arranged by size: 5631 434 400 306 95 14 PstI C_TGCA'G Cuts at: 830 2091 2364 4017 4232 830 Size: 1261 273 1653 215 3478 Fragments arranged by size: 3478 1653 1261 273 215 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6880 PvuII CAG'CTG Cuts at: 2461 2725 2798 2461 Size: 264 73 6543 Fragments arranged by size: 6543 264 73 SacI G_AGCT'C Cuts at: 2646 3609 4080 2646 Size: 963 471 5446 Fragments arranged by size: 5446 963 471 SalI G'TCGA_C Cuts at: 1936 4222 1936 Size: 2286 4594 SanDI GG'GwC_CC Cuts at: 2187 2739 2187 Size: 552 6328 SapI GCTCTTCn'nnn_ Cuts at: 3185 3899 3185 Size: 714 6166 SbfI CC_TGCA'GG Cuts at: 4232 4232 Size: 6880 ScaI AGT'ACT Cuts at: 5128 6840 5128 Size: 1712 5168 SexAI A'CCwGG_T Cuts at: 2652 2652 Size: 6880 SfcI C'TryA_G Cuts at: 826 1045 2087 2360 2419 3212 4013 4228 5363 Size: 219 1042 273 59 793 801 215 1135 Cuts at: 5363 6044 6235 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1042 801 793 681 273 219 215 191 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6880 SmaI CCC'GGG Cuts at: 1872 3656 3752 3820 4207 1872 Size: 1784 96 68 387 4545 Fragments arranged by size: 4545 1784 387 96 68 SmlI C'TyrA_G Cuts at: 820 1017 1943 4984 5885 6147 820 Size: 197 926 3041 901 262 1553 Fragments arranged by size: 3041 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4671 493 Size: 4178 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6880 SphI G_CATG'C Cuts at: 1132 4238 1132 Size: 3106 3774 SrfI GCCC'GGGC Cuts at: 3752 3752 Size: 6880 SspI AAT'ATT Cuts at: 5 52 4804 5 Size: 47 4752 2081 Fragments arranged by size: 4752 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6880 StyI C'CwwG_G Cuts at: 513 1065 2004 2136 2206 2745 2884 3018 3612 Size: 552 939 132 70 539 139 134 594 Cuts at: 3612 3963 4117 4319 513 Size: 351 154 202 3074 Fragments arranged by size: 3074 939 594 552 539 351 202 154 139 134 132 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 3002 3744 4166 4907 4924 5077 Size: 1255 633 789 742 422 741 17 153 Cuts at: 5077 5262 5707 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 789 742 741 633 445 422 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5126 6838 96 Size: 274 80 33 51 1443 3149 1712 138 Fragments arranged by size: 3149 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3251 3283 6840 6872 3251 Size: 32 3557 32 3259 Fragments arranged by size: 3557 3259 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2708 1316 Size: 1392 5488 XbaI T'CTAG_A Cuts at: 4216 4216 Size: 6880 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6880 XmaI C'CCGG_G Cuts at: 1870 3654 3750 3818 4205 1870 Size: 1784 96 68 387 4545 Fragments arranged by size: 4545 1784 387 96 68 XmnI GAAnn'nnTTC Cuts at: 2306 2546 4022 5009 2306 Size: 240 1476 987 4177 Fragments arranged by size: 4177 1476 987 240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4688 276 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SwaI XcmI