DCDC2 ORF clone pFN21AA1154

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Halotag ORF Clone pFN21AA1154

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Clone info.

Species

human

Clone Name

pFN21AA1154 Sequence (with vector) Restriction Map

Product ID

FHC00190

Flexi Clone PID

FXC00190

ORF Sequence

DNA sequence (1428 bp)
Protein sequence (476 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385433]

Gene

KIAA1154

Symbol

DCDC2

Alias : DCDC2A, DFNB66, NPHP19, NSC, RU2, RU2S

Description

doublecortin domain containing 2, transcript variant 1

Original Clone ID

hh03679s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1428 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 476 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4550.1 7.4e-125 99.8 DCDC2 CCDS74481.1 4.7e-19 33.7 DCDC2C CCDS44100.1 2.4e-17 38.1 DCDC2B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003533 34 95 PF03607 Doublecortin domain IPR003533 156 216 PF03607 Doublecortin domain HMMSmart IPR003533 12 100 SM00537 Doublecortin domain IPR003533 134 221 SM00537 Doublecortin domain ProfileScan IPR003533 17 100 PS50309 Doublecortin domain IPR003533 139 221 PS50309 Doublecortin domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001182539 476 605755,610212,610212,616217 99.8 100.0 NP_057440 476 605755,610212,610212,616217 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1154 Download

>KIAA1154  1428 bp
ATGAGCGGCAGCAGCGCCAGGTCCAGCCACCTGTCTCAGCCCGTCGTGAAGAGCGTGCTT
GTGTACCGCAACGGGGACCCCTTCTACGCGGGGCGCCGCGTCGTCATCCATGAGAAGAAG
GTGTCCAGCTTCGAAGTCTTCCTGAAGGAGGTGACCGGCGGCGTTCAGGCACCCTTTGGG
GCCGTCAGGAACATCTACACCCCGCGGACTGGCCACCGAATCCGGAAGCTAGACCAGATC
CAGAGCGGGGGCAATTACGTGGCTGGAGGCCAGGAAGCCTTCAAGAAACTCAATTACTTG
GACATAGGAGAAATCAAGAAAAGACCAATGGAAGTTGTTAATACAGAGGTAAAACCAGTA
ATCCATAGCAGGATCAACGTGTCAGCTCGCTTTAGAAAACCGCTTCAGGAGCCGTGCACT
ATCTTCTTGATTGCAAATGGAGACCTCATAAACCCAGCTTCTCGCCTCCTTATCCCCAGA
AAAACCTTGAATCAGTGGGATCATGTACTACAAATGGTCACAGAAAAAATCACTCTGAGG
AGCGGGGCTGTTCACAGGCTTTATACTTTAGAAGGAAAACTTGTTGAGAGTGGAGCAGAG
TTGGAGAATGGGCAGTTTTATGTGGCTGTTGGCAGAGATAAGTTTAAGAAACTGCCTTAC
GGTGAGTTACTTTTTGACAAGTCAACGATGAGAAGGCCTTTTGGTCAGAAAGCTTCTTCA
CTACCTCCTATTGTAGGATCCAGAAAGTCTAAAGGGAGTGGAAATGATCGCCACTCTAAG
TCAACAGTTGGATCCAGTGACAACTCATCTCCTCAGCCCCTGAAGAGGAAAGGGAAAAAA
GAAGACGTGAATTCAGAAAAACTGACGAAATTGAAACAAAATGTAAAATTAAAGAATTCA
CAAGAAACCATTCCAAATAGTGATGAAGGCATTTTCAAAGCTGGAGCAGAGAGGTCTGAA
ACACGGGGGGCAGCAGAAGTCCAAGAAGATGAAGATACTCAGGTTGAGGTTCCAGTCGAT
CAGAGGCCAGCAGAAATAGTAGACGAGGAAGAAGATGGAGAGAAGGCAAACAAGGATGCA
GAACAGAAAGAAGACTTTTCAGGAATGAATGGTGACCTTGAAGAGGAAGGAGGTAGGGAG
GCTACAGATGCCCCTGAGCAAGTCGAGGAGATTCTGGATCACAGTGAGCAGCAGGCACGC
CCTGCTCGTGTAAATGGAGGCACCGATGAGGAGAATGGTGAGGAGCTGCAGCAGGTTAAT
AATGAGCTTCAACTGGTCCTAGACAAGGAAAGAAAGTCTCAAGGAGCTGGCAGTGGACAA
GATGAGGCTGATGTAGACCCTCAAAGACCACCAAGGCCAGAAGTAAAAATTACCAGTCCA
GAAGAAAATGAAAACAACCAACAAAACAAGGACTATGCTGCCGTGGCT

ORF Protein

Cloned ORF protein sequence for pFN21AA1154 Download

>KIAA1154  476 aa
MSGSSARSSHLSQPVVKSVLVYRNGDPFYAGRRVVIHEKKVSSFEVFLKEVTGGVQAPFG
AVRNIYTPRTGHRIRKLDQIQSGGNYVAGGQEAFKKLNYLDIGEIKKRPMEVVNTEVKPV
IHSRINVSARFRKPLQEPCTIFLIANGDLINPASRLLIPRKTLNQWDHVLQMVTEKITLR
SGAVHRLYTLEGKLVESGAELENGQFYVAVGRDKFKKLPYGELLFDKSTMRRPFGQKASS
LPPIVGSRKSKGSGNDRHSKSTVGSSDNSSPQPLKRKGKKEDVNSEKLTKLKQNVKLKNS
QETIPNSDEGIFKAGAERSETRGAAEVQEDEDTQVEVPVDQRPAEIVDEEEDGEKANKDA
EQKEDFSGMNGDLEEEGGREATDAPEQVEEILDHSEQQARPARVNGGTDEENGEELQQVN
NELQLVLDKERKSQGAGSGQDEADVDPQRPPRPEVKITSPEENENNQQNKDYAAVA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1154 Download

>pFN21AA1154  6133 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCGGCAGCAGCGCCAGGTCCAGCCACCTGTC
TCAGCCCGTCGTGAAGAGCGTGCTTGTGTACCGCAACGGGGACCCCTTCTACGCGGGGCG
CCGCGTCGTCATCCATGAGAAGAAGGTGTCCAGCTTCGAAGTCTTCCTGAAGGAGGTGAC
CGGCGGCGTTCAGGCACCCTTTGGGGCCGTCAGGAACATCTACACCCCGCGGACTGGCCA
CCGAATCCGGAAGCTAGACCAGATCCAGAGCGGGGGCAATTACGTGGCTGGAGGCCAGGA
AGCCTTCAAGAAACTCAATTACTTGGACATAGGAGAAATCAAGAAAAGACCAATGGAAGT
TGTTAATACAGAGGTAAAACCAGTAATCCATAGCAGGATCAACGTGTCAGCTCGCTTTAG
AAAACCGCTTCAGGAGCCGTGCACTATCTTCTTGATTGCAAATGGAGACCTCATAAACCC
AGCTTCTCGCCTCCTTATCCCCAGAAAAACCTTGAATCAGTGGGATCATGTACTACAAAT
GGTCACAGAAAAAATCACTCTGAGGAGCGGGGCTGTTCACAGGCTTTATACTTTAGAAGG
AAAACTTGTTGAGAGTGGAGCAGAGTTGGAGAATGGGCAGTTTTATGTGGCTGTTGGCAG
AGATAAGTTTAAGAAACTGCCTTACGGTGAGTTACTTTTTGACAAGTCAACGATGAGAAG
GCCTTTTGGTCAGAAAGCTTCTTCACTACCTCCTATTGTAGGATCCAGAAAGTCTAAAGG
GAGTGGAAATGATCGCCACTCTAAGTCAACAGTTGGATCCAGTGACAACTCATCTCCTCA
GCCCCTGAAGAGGAAAGGGAAAAAAGAAGACGTGAATTCAGAAAAACTGACGAAATTGAA
ACAAAATGTAAAATTAAAGAATTCACAAGAAACCATTCCAAATAGTGATGAAGGCATTTT
CAAAGCTGGAGCAGAGAGGTCTGAAACACGGGGGGCAGCAGAAGTCCAAGAAGATGAAGA
TACTCAGGTTGAGGTTCCAGTCGATCAGAGGCCAGCAGAAATAGTAGACGAGGAAGAAGA
TGGAGAGAAGGCAAACAAGGATGCAGAACAGAAAGAAGACTTTTCAGGAATGAATGGTGA
CCTTGAAGAGGAAGGAGGTAGGGAGGCTACAGATGCCCCTGAGCAAGTCGAGGAGATTCT
GGATCACAGTGAGCAGCAGGCACGCCCTGCTCGTGTAAATGGAGGCACCGATGAGGAGAA
TGGTGAGGAGCTGCAGCAGGTTAATAATGAGCTTCAACTGGTCCTAGACAAGGAAAGAAA
GTCTCAAGGAGCTGGCAGTGGACAAGATGAGGCTGATGTAGACCCTCAAAGACCACCAAG
GCCAGAAGTAAAAATTACCAGTCCAGAAGAAAATGAAAACAACCAACAAAACAAGGACTA
TGCTGCCGTGGCTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT