(Circular) (Six-base) MAPSORT of: pFN21AA1154.seq Check: 3048 from: 1 to: 6133 pFN21AA1154 6133 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3045 3339 3476 1937 Size: 1108 294 137 4594 Fragments arranged by size: 4594 1108 294 137 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2168 2393 2846 4196 5580 5848 1632 Size: 536 225 453 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1350 536 453 268 225 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 2382 5032 6009 1203 Size: 1179 2650 977 1327 Fragments arranged by size: 2650 1327 1179 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 2824 3876 5448 2824 Size: 1052 1572 3509 Fragments arranged by size: 3509 1572 1052 ApaLI G'TGCA_C Cuts at: 2419 4190 5346 2419 Size: 1771 1156 3206 Fragments arranged by size: 3206 1771 1156 ApoI r'AATT_y Cuts at: 1493 2854 2899 3442 3668 3820 5929 1493 Size: 1361 45 543 226 152 2109 1697 Fragments arranged by size: 2109 1697 1361 543 226 152 45 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 3458 1870 Size: 73 1515 4545 Fragments arranged by size: 4545 1515 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2991 3024 2991 Size: 33 6100 BamHI G'GATC_C Cuts at: 2741 2795 3463 3830 2741 Size: 54 668 367 5044 Fragments arranged by size: 5044 668 367 54 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2097 2173 3224 3454 4909 Size: 325 217 627 310 76 1051 230 1455 Cuts at: 4909 618 Size: 1842 Fragments arranged by size: 1842 1455 1051 627 325 310 230 217 76 BanII G_rGCy'C Cuts at: 1391 3452 1391 Size: 2061 4072 BbeI G_GCGC'C Cuts at: 2101 2101 Size: 6133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2134 2853 3102 928 Size: 971 235 719 249 3959 Fragments arranged by size: 3959 971 719 249 235 BbvCI CC'TCA_GC Cuts at: 2817 2817 Size: 6133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4324 4358 1903 Size: 34 2387 34 3678 Fragments arranged by size: 3678 2387 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4744 136 Size: 107 122 71 631 605 3072 1525 Fragments arranged by size: 3072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6128 6128 Size: 6133 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2423 4194 5350 1790 Size: 633 1771 1156 2573 Fragments arranged by size: 2573 1771 1156 633 BmgBI CAC'GTC Cuts at: 1318 2851 1318 Size: 1533 4600 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3864 4822 442 Size: 416 53 83 399 24 2447 958 1753 Fragments arranged by size: 2447 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2289 2967 4793 1239 Size: 1050 678 1826 2579 Fragments arranged by size: 2579 1826 1050 678 Bpu10I CC'TnA_GC Cuts at: 2817 3159 2817 Size: 342 5791 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3288 4258 5123 5421 3288 Size: 970 865 298 4000 Fragments arranged by size: 4000 970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 4796 882 Size: 617 940 2357 2219 Fragments arranged by size: 2357 2219 940 617 BsaAI yAC'GTr Cuts at: 493 1204 2263 3924 493 Size: 711 1059 1661 2702 Fragments arranged by size: 2702 1661 1059 711 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2098 3940 4322 4957 Size: 53 83 186 936 565 1842 382 635 Cuts at: 4957 275 Size: 1451 Fragments arranged by size: 1842 1451 936 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2226 4566 4964 5238 5385 5796 2226 Size: 2340 398 274 147 411 2563 Fragments arranged by size: 2563 2340 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5908 Fragments arranged by size: 5908 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2557 2805 3185 3248 3260 1186 Size: 1371 248 380 63 12 4059 Fragments arranged by size: 4059 1371 380 248 63 12 BseYI C'CCAG_C Cuts at: 2458 5336 5832 2458 Size: 2878 496 2759 Fragments arranged by size: 2878 2759 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2423 4194 4279 5350 2423 Size: 1771 85 1071 3206 Fragments arranged by size: 3206 1771 1071 85 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2423 3452 4194 4279 5350 1391 Size: 399 633 1029 742 85 1071 2174 Fragments arranged by size: 2174 1071 1029 742 633 399 85 BspEI T'CCGG_A Cuts at: 2226 5796 2226 Size: 3570 2563 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3247 3488 844 Size: 2403 241 3489 Fragments arranged by size: 3489 2403 241 BsrBI CCG'CTC Cuts at: 1394 1823 2010 2250 2547 4020 1394 Size: 429 187 240 297 1473 3507 Fragments arranged by size: 3507 1473 429 297 240 187 BsrDI GCAATG_nn' Cuts at: 65 1248 4628 4802 65 Size: 1183 3380 174 1396 Fragments arranged by size: 3380 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2159 4777 4964 1700 Size: 110 349 2618 187 2869 Fragments arranged by size: 2869 2618 349 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 3210 4193 5205 3210 Size: 983 1012 4138 Fragments arranged by size: 4138 1012 983 BstAPI GCAn_nnn'nTGC Cuts at: 794 3206 794 Size: 2412 3721 BstBI TT'CG_AA Cuts at: 2136 4937 2136 Size: 2801 3332 BstEII G'GTnAC_C Cuts at: 1184 2155 3116 1184 Size: 971 961 4201 Fragments arranged by size: 4201 971 961 BstYI r'GATC_y Cuts at: 1972 2241 2741 2795 3463 3830 4215 4232 5684 Size: 269 500 54 668 367 385 17 1452 Cuts at: 5684 5770 5782 6128 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 668 500 385 367 346 269 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2207 3426 513 Size: 552 1142 1219 3220 Fragments arranged by size: 3220 1219 1142 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 3322 3649 4443 4463 3322 Size: 327 794 20 4992 Fragments arranged by size: 4992 794 327 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 3437 3437 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 3580 4469 Size: 54 1425 50 275 18 385 1365 889 Cuts at: 4469 6098 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1365 889 385 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2822 3120 4063 1353 Size: 695 774 298 943 3423 Fragments arranged by size: 3423 943 774 695 298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2289 2393 2846 2967 4196 4793 Size: 393 536 121 104 453 121 1229 597 Cuts at: 4793 5580 5848 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1229 787 597 536 453 393 268 121 121 104 EcoNI CCTnn'n_nnAGG Cuts at: 2150 2736 3125 3287 2150 Size: 586 389 162 4996 Fragments arranged by size: 4996 586 389 162 EcoO109I rG'GnC_Cy Cuts at: 2080 2080 Size: 6133 EcoRI G'AATT_C Cuts at: 2854 2899 3442 3820 2854 Size: 45 543 378 5167 Fragments arranged by size: 5167 543 378 45 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2600 2600 Size: 6133 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 2022 2101 5280 1678 Size: 344 79 3179 2531 Fragments arranged by size: 3179 2531 344 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2762 2794 2795 2827 3166 Size: 32 135 32 1190 32 1 32 339 Cuts at: 3166 3198 4774 4806 4848 4880 1373 Size: 32 1576 32 42 32 2626 Fragments arranged by size: 2626 1576 1190 339 135 42 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2688 2787 3477 3723 669 Size: 988 281 750 99 690 246 3079 Fragments arranged by size: 3079 988 750 690 281 246 99 HindIII A'AGCT_T Cuts at: 748 2715 748 Size: 1967 4166 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KasI G'GCGC_C Cuts at: 2097 2097 Size: 6133 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2586 2773 5247 5431 1305 Size: 1281 187 2474 184 2007 Fragments arranged by size: 2474 2007 1281 187 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2217 10 Size: 54 1425 343 385 3926 Fragments arranged by size: 3926 1425 385 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4091 4450 4609 518 Size: 648 2925 359 159 2042 Fragments arranged by size: 2925 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2209 3549 4226 5374 5619 1606 Size: 603 1340 677 1148 245 2120 Fragments arranged by size: 2120 1340 1148 677 603 245 NarI GG'CG_CC Cuts at: 2098 2098 Size: 6133 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5581 NdeI CA'TA_TG Cuts at: 387 387 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 3491 5036 1132 Size: 97 2262 1545 2229 Fragments arranged by size: 2262 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6133 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2080 Size: 6133 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6133 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PstI C_TGCA'G Cuts at: 830 3255 3485 830 Size: 2425 230 3478 Fragments arranged by size: 3478 2425 230 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 SacII CC_GC'GG Cuts at: 2210 2210 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SanDI GG'GwC_CC Cuts at: 2080 2080 Size: 6133 SapI GCTCTTCn'nnn_ Cuts at: 2048 2048 Size: 6133 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SfcI C'TryA_G Cuts at: 826 1045 3147 3251 3481 4616 5297 5488 826 Size: 219 2102 104 230 1135 681 191 1471 Fragments arranged by size: 2102 1471 1135 681 230 219 191 104 SfoI GGC'GCC Cuts at: 2099 2099 Size: 6133 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3303 4237 5138 5400 820 Size: 197 926 1360 934 901 262 1553 Fragments arranged by size: 1553 1360 934 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 2701 1517 Size: 1184 4949 StyI C'CwwG_G Cuts at: 513 1065 3356 3572 513 Size: 552 2291 216 3074 Fragments arranged by size: 3074 2291 552 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4160 4177 4330 4515 4960 325 Size: 1255 2580 17 153 185 445 1498 Fragments arranged by size: 2580 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2509 4379 6091 Size: 274 80 33 51 1443 532 1870 1712 Cuts at: 6091 96 Size: 138 Fragments arranged by size: 1870 1712 1443 532 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6093 6125 6093 Size: 32 6101 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 2913 4262 2913 Size: 1349 4784 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SexAI SfiI SrfI SwaI XcmI