ARHGAP26 ORF clone pFN21AA0621

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AA0621

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Clone info.

Species

human

Clone Name

pFN21AA0621 Sequence (with vector) Restriction Map

Product ID

FHC01986

Flexi Clone PID

FXC01986

Original Clone PID

ORK01986

ORF Sequence

DNA sequence (2166 bp)
Protein sequence (722 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463225]

Gene

KIAA0621

Symbol

ARHGAP26

Alias : GRAF, GRAF1, OPHN1L, OPHN1L1

Description

Rho GTPase activating protein 26

Original Clone ID

fg03084

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2166 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 722 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS47297.1 6.3e-171 95.1 ARHGAP26 CCDS4277.1 6.6e-171 99.8 ARHGAP26 CCDS34075.1 3.8e-99 56.1 ARHGAP10 CCDS14388.1 5.8e-83 51.4 OPHN1 CCDS2184.1 1.4e-12 35.2 ARHGAP15 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013606 34 217 PF08397 IRSp53/MIM homology domain (IMD) IPR000198 392 542 PF00620 Rho GTPase-activating protein domain NULL 670 715 PF00018 NULL HMMSmart IPR001849 266 371 SM00233 Pleckstrin homology domain IPR000198 387 565 SM00324 Rho GTPase-activating protein domain NULL 667 722 SM00326 NULL ProfileScan IPR001849 265 369 PS50003 Pleckstrin homology domain IPR000198 383 568 PS50238 Rho GTPase-activating protein domain IPR001452 664 722 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005268459 722 605370,607785 100.0 100.0 XP_005268457 765 605370,607785 99.9 93.1 NP_001129080 759 605370,607785 95.1 99.3 NP_055886 814 605370,607785 99.8 92.1 XP_005268456 802 605370,607785 94.6 92.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0621 Download

>KIAA0621  2166 bp
ATGGGGCTCCCAGCGCTCGAGTTCAGCGACTGCTGCCTCGATAGTCCGCACTTCCGAGAG
ACGCTCAAGTCGCACGAAGCAGAGCTGGACAAGACCAACAAATTCATCAAGGAGCTCATC
AAGGACGGGAAGTCACTCATAAGCGCGCTCAAGAATTTGTCTTCAGCGAAGCGGAAGTTT
GCAGATTCCTTAAATGAATTTAAATTTCAGTGCATAGGAGATGCAGAAACAGATGATGAG
ATGTGTATAGCAAGGTCTTTGCAGGAGTTTGCCACTGTCCTCAGGAATCTTGAAGATGAA
CGGATACGGATGATTGAGAATGCCAGCGAGGTGCTCATCACTCCCTTGGAGAAGTTTCGA
AAGGAACAGATCGGGGCTGCCAAGGAAGCCAAAAAGAAGTATGACAAAGAGACAGAAAAG
TATTGTGGCATCTTAGAAAAACACTTGAATTTGTCTTCCAAAAAGAAAGAATCTCAGCTT
CAGGAGGCAGACAGCCAAGTGGACCTGGTCCGGCAGCATTTCTATGAAGTATCCCTGGAA
TATGTCTTCAAGGTGCAGGAAGTCCAAGAGAGAAAGATGTTTGAGTTTGTGGAGCCTCTG
CTGGCCTTCCTGCAAGGACTCTTCACTTTCTATCACCATGGTTACGAACTGGCCAAGGAT
TTCGGGGACTTCAAGACACAGTTAACCATTAGCATACAGAACACAAGAAATCGCTTTGAA
GGCACTAGATCAGAAGTGGAATCACTGATGAAAAAGATGAAGGAGAATCCCCTTGAGCAC
AAGACCATCAGTCCCTACACCATGGAGGGATACCTCTACGTGCAGGAGAAACGTCACTTT
GGAACTTCTTGGGTGAAGCACTACTGTACATATCAACGGGATTCCAAACAAATCACCATG
GTACCATTTGACCAAAAGTCAGGAGGAAAAGGGGGAGAAGATGAATCAGTTATCCTCAAA
TCCTGCACACGGCGGAAAACAGACTCCATTGAGAAGAGGTTTTGCTTTGATGTGGAAGCA
GTAGACAGGCCAGGGGTTATCACCATGCAAGCTTTGTCGGAAGAGGACCGGAGGCTCTGG
ATGGAAGCCATGGATGGCCGGGAACCTGTCTACAACTCGAACAAAGACAGCCAGAGTGAA
GGGACTGCGCAGTTGGACAGCATTGGCTTCAGCATAATCAGGAAATGCATCCATGCTGTG
GAAACCAGAGGGATCAACGAGCAAGGGCTGTATCGAATTGTGGGGGTCAACTCCAGAGTG
CAGAAGTTGCTGAGTGTCCTGATGGACCCCAAGACTGCTTCTGAGACAGAAACAGATATC
TGTGCTGAATGGGAGATAAAGACCATCACTAGTGCTCTGAAGACCTACCTAAGAATGCTT
CCAGGACCACTCATGATGTACCAGTTTCAAAGAAGTTTCATCAAAGCAGCAAAACTGGAG
AACCAGGAGTCTCGGGTCTCTGAAATCCACAGCCTTGTTCATCGGCTCCCAGAGAAAAAT
CGGCAGATGTTACAGCTGCTCATGAACCACTTGGCAAATGTTGCTAACAACCACAAGCAG
AATTTGATGACGGTGGCAAACCTTGGTGTGGTGTTTGGACCCACTCTGCTGAGGCCTCAG
GAAGAAACAGTAGCAGCCATCATGGACATCAAATTTCAGAACATTGTCATTGAGATCCTA
ATAGAAAACCACGAAAAGATATTTAACACCGTGCCCGATATGCCTCTCACCAATGCCCAG
CTGCACCTGTCTCGGAAGAAGAGCAGTGACTCCAAGCCCCCGTCCTGCAGCGAGAGGCCC
CTGACGCTCTTCCACACCGTTCAGTCAACAGAGAAACAGGAACAAAGGAACAGCATCATC
AACTCCAGTTTGGAATCTGTCTCATCAAATCCAAACAGCATCCTTAATTCCAGCAGCAGC
TTACAGCCCAACATGAACTCCAGTGACCCAGACCTGGCTGTGGTCAAACCCACCCGGCCC
AACTCACTCACACCGTTCCGGAAGGCAAAAGCCTTGTATGCCTGCAAAGCTGAACATGAC
TCAGAACTTTCGTTCACAGCAGGCACGGTCTTCGATAATGTTCACCCATCTCAGGAGCCT
GGCTGGTTGGAGGGGACTCTGAACGGAAAGACTGGCCTCATCCCTGAGAATTACGTGGAG
TTCCTC

ORF Protein

Cloned ORF protein sequence for pFN21AA0621 Download

>KIAA0621  722 aa
MGLPALEFSDCCLDSPHFRETLKSHEAELDKTNKFIKELIKDGKSLISALKNLSSAKRKF
ADSLNEFKFQCIGDAETDDEMCIARSLQEFATVLRNLEDERIRMIENASEVLITPLEKFR
KEQIGAAKEAKKKYDKETEKYCGILEKHLNLSSKKKESQLQEADSQVDLVRQHFYEVSLE
YVFKVQEVQERKMFEFVEPLLAFLQGLFTFYHHGYELAKDFGDFKTQLTISIQNTRNRFE
GTRSEVESLMKKMKENPLEHKTISPYTMEGYLYVQEKRHFGTSWVKHYCTYQRDSKQITM
VPFDQKSGGKGGEDESVILKSCTRRKTDSIEKRFCFDVEAVDRPGVITMQALSEEDRRLW
MEAMDGREPVYNSNKDSQSEGTAQLDSIGFSIIRKCIHAVETRGINEQGLYRIVGVNSRV
QKLLSVLMDPKTASETETDICAEWEIKTITSALKTYLRMLPGPLMMYQFQRSFIKAAKLE
NQESRVSEIHSLVHRLPEKNRQMLQLLMNHLANVANNHKQNLMTVANLGVVFGPTLLRPQ
EETVAAIMDIKFQNIVIEILIENHEKIFNTVPDMPLTNAQLHLSRKKSSDSKPPSCSERP
LTLFHTVQSTEKQEQRNSIINSSLESVSSNPNSILNSSSSLQPNMNSSDPDLAVVKPTRP
NSLTPFRKAKALYACKAEHDSELSFTAGTVFDNVHPSQEPGWLEGTLNGKTGLIPENYVE
FL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0621 Download

>pFN21AA0621  6871 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGCTCCCAGCGCTCGAGTTCAGCGACTGCTG
CCTCGATAGTCCGCACTTCCGAGAGACGCTCAAGTCGCACGAAGCAGAGCTGGACAAGAC
CAACAAATTCATCAAGGAGCTCATCAAGGACGGGAAGTCACTCATAAGCGCGCTCAAGAA
TTTGTCTTCAGCGAAGCGGAAGTTTGCAGATTCCTTAAATGAATTTAAATTTCAGTGCAT
AGGAGATGCAGAAACAGATGATGAGATGTGTATAGCAAGGTCTTTGCAGGAGTTTGCCAC
TGTCCTCAGGAATCTTGAAGATGAACGGATACGGATGATTGAGAATGCCAGCGAGGTGCT
CATCACTCCCTTGGAGAAGTTTCGAAAGGAACAGATCGGGGCTGCCAAGGAAGCCAAAAA
GAAGTATGACAAAGAGACAGAAAAGTATTGTGGCATCTTAGAAAAACACTTGAATTTGTC
TTCCAAAAAGAAAGAATCTCAGCTTCAGGAGGCAGACAGCCAAGTGGACCTGGTCCGGCA
GCATTTCTATGAAGTATCCCTGGAATATGTCTTCAAGGTGCAGGAAGTCCAAGAGAGAAA
GATGTTTGAGTTTGTGGAGCCTCTGCTGGCCTTCCTGCAAGGACTCTTCACTTTCTATCA
CCATGGTTACGAACTGGCCAAGGATTTCGGGGACTTCAAGACACAGTTAACCATTAGCAT
ACAGAACACAAGAAATCGCTTTGAAGGCACTAGATCAGAAGTGGAATCACTGATGAAAAA
GATGAAGGAGAATCCCCTTGAGCACAAGACCATCAGTCCCTACACCATGGAGGGATACCT
CTACGTGCAGGAGAAACGTCACTTTGGAACTTCTTGGGTGAAGCACTACTGTACATATCA
ACGGGATTCCAAACAAATCACCATGGTACCATTTGACCAAAAGTCAGGAGGAAAAGGGGG
AGAAGATGAATCAGTTATCCTCAAATCCTGCACACGGCGGAAAACAGACTCCATTGAGAA
GAGGTTTTGCTTTGATGTGGAAGCAGTAGACAGGCCAGGGGTTATCACCATGCAAGCTTT
GTCGGAAGAGGACCGGAGGCTCTGGATGGAAGCCATGGATGGCCGGGAACCTGTCTACAA
CTCGAACAAAGACAGCCAGAGTGAAGGGACTGCGCAGTTGGACAGCATTGGCTTCAGCAT
AATCAGGAAATGCATCCATGCTGTGGAAACCAGAGGGATCAACGAGCAAGGGCTGTATCG
AATTGTGGGGGTCAACTCCAGAGTGCAGAAGTTGCTGAGTGTCCTGATGGACCCCAAGAC
TGCTTCTGAGACAGAAACAGATATCTGTGCTGAATGGGAGATAAAGACCATCACTAGTGC
TCTGAAGACCTACCTAAGAATGCTTCCAGGACCACTCATGATGTACCAGTTTCAAAGAAG
TTTCATCAAAGCAGCAAAACTGGAGAACCAGGAGTCTCGGGTCTCTGAAATCCACAGCCT
TGTTCATCGGCTCCCAGAGAAAAATCGGCAGATGTTACAGCTGCTCATGAACCACTTGGC
AAATGTTGCTAACAACCACAAGCAGAATTTGATGACGGTGGCAAACCTTGGTGTGGTGTT
TGGACCCACTCTGCTGAGGCCTCAGGAAGAAACAGTAGCAGCCATCATGGACATCAAATT
TCAGAACATTGTCATTGAGATCCTAATAGAAAACCACGAAAAGATATTTAACACCGTGCC
CGATATGCCTCTCACCAATGCCCAGCTGCACCTGTCTCGGAAGAAGAGCAGTGACTCCAA
GCCCCCGTCCTGCAGCGAGAGGCCCCTGACGCTCTTCCACACCGTTCAGTCAACAGAGAA
ACAGGAACAAAGGAACAGCATCATCAACTCCAGTTTGGAATCTGTCTCATCAAATCCAAA
CAGCATCCTTAATTCCAGCAGCAGCTTACAGCCCAACATGAACTCCAGTGACCCAGACCT
GGCTGTGGTCAAACCCACCCGGCCCAACTCACTCACACCGTTCCGGAAGGCAAAAGCCTT
GTATGCCTGCAAAGCTGAACATGACTCAGAACTTTCGTTCACAGCAGGCACGGTCTTCGA
TAATGTTCACCCATCTCAGGAGCCTGGCTGGTTGGAGGGGACTCTGAACGGAAAGACTGG
CCTCATCCCTGAGAATTACGTGGAGTTCCTCGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT