(Circular) (Six-base) MAPSORT of: pFN21AA0621.seq Check: 1674 from: 1 to: 6871 pFN21AA0621 6871 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4681 278 Size: 53 83 186 936 3145 2468 Fragments arranged by size: 3145 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3027 3114 4214 1937 Size: 1090 87 1100 4594 Fragments arranged by size: 4594 1100 1090 87 Acc65I G'GTAC_C Cuts at: 2905 4192 2905 Size: 1287 5584 AclI AA'CG_TT Cuts at: 1563 4998 5371 1563 Size: 3435 373 3063 Fragments arranged by size: 3435 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 2468 3157 3383 4934 6318 6586 1632 Size: 519 317 689 226 1551 1384 268 1917 Fragments arranged by size: 1917 1551 1384 689 519 317 268 226 AfeI AGC'GCT Cuts at: 2019 2019 Size: 6871 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6674 AflIII A'CryG_T Cuts at: 1203 5770 6747 1203 Size: 4567 977 1327 Fragments arranged by size: 4567 1327 977 AgeI A'CCGG_T Cuts at: 5702 5702 Size: 6871 AhdI GACnn_n'nnGTC Cuts at: 2134 2793 2920 5600 2134 Size: 659 127 2680 3405 Fragments arranged by size: 3405 2680 659 127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6871 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2289 1184 Size: 1105 5766 AlwNI CAG_nnn'CTG Cuts at: 2034 4614 6186 2034 Size: 2580 1572 2719 Fragments arranged by size: 2719 2580 1572 ApaLI G'TGCA_C Cuts at: 4928 6084 4928 Size: 1156 5715 ApoI r'AATT_y Cuts at: 1493 2105 2158 2201 2207 2452 3565 3656 4180 Size: 612 53 43 6 245 1113 91 524 Cuts at: 4180 4406 4558 6667 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1113 612 524 245 226 152 91 53 43 6 AseI AT'TA_AT Cuts at: 160 1032 5425 160 Size: 872 4393 1606 Fragments arranged by size: 4393 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6871 AvaI C'yCGr_G Cuts at: 1870 1943 2021 3456 4196 1870 Size: 73 78 1435 740 4545 Fragments arranged by size: 4545 1435 740 78 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2889 2922 2889 Size: 33 6838 BamHI G'GATC_C Cuts at: 4201 4568 4201 Size: 367 6504 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2905 4192 5647 618 Size: 325 217 627 1118 1287 1455 1842 Fragments arranged by size: 1842 1455 1287 1118 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2121 4190 1391 Size: 622 108 2069 4072 Fragments arranged by size: 4072 2069 622 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2157 2451 2542 3371 4066 928 Size: 971 258 294 91 829 695 3733 Fragments arranged by size: 3733 971 829 695 294 258 91 BbvCI CC'TCA_GC Cuts at: 3614 3614 Size: 6871 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3737 3771 4057 4091 5062 5096 1903 Size: 34 1800 34 286 34 971 34 3678 Fragments arranged by size: 3678 1800 971 286 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2301 2545 2807 4763 5973 2301 Size: 244 262 1956 1210 3199 Fragments arranged by size: 3199 1956 1210 262 244 BclI T'GATC_A Cuts at: 1548 1548 Size: 6871 BfrBI ATG'CAT Cuts at: 3192 3192 Size: 6871 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5482 136 Size: 107 122 71 631 605 3810 1525 Fragments arranged by size: 3810 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6866 6866 Size: 6871 BlpI GC'TnA_GC Cuts at: 4288 4288 Size: 6871 Bme1580I G_kGCm'C Cuts at: 1790 3720 4932 6088 1790 Size: 1930 1212 1156 2573 Fragments arranged by size: 2573 1930 1212 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6871 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4602 5560 442 Size: 416 53 83 399 24 3185 958 1753 Fragments arranged by size: 3185 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6871 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2084 2084 Size: 6871 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3241 3461 3853 3928 5531 1239 Size: 2002 220 392 75 1603 2579 Fragments arranged by size: 2579 2002 1603 392 220 75 Bpu10I CC'TnA_GC Cuts at: 3614 3614 Size: 6871 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 2138 2798 4996 5861 6159 2054 Size: 84 660 2198 865 298 2766 Fragments arranged by size: 2766 2198 865 660 298 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3466 5534 882 Size: 617 1967 2068 2219 Fragments arranged by size: 2219 2068 1967 617 BsaAI yAC'GTr Cuts at: 493 1204 2824 4159 4662 493 Size: 711 1620 1335 503 2702 Fragments arranged by size: 2702 1620 1335 711 503 BsaBI GATnn'nnATC Cuts at: 6865 6865 Size: 6871 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4678 5060 5695 275 Size: 53 83 186 936 3145 382 635 1451 Fragments arranged by size: 3145 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3072 4002 5304 5702 5976 6123 6534 3072 Size: 930 1302 398 274 147 411 3409 Fragments arranged by size: 3409 1302 930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3926 3956 1178 Size: 30 165 30 2523 30 4093 Fragments arranged by size: 4093 2523 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6871 BseYI C'CCAG_C Cuts at: 2014 3741 6074 6570 2014 Size: 1727 2333 496 2315 Fragments arranged by size: 2333 2315 1727 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2579 2846 2953 3284 3731 1163 Size: 573 843 267 107 331 447 4303 Fragments arranged by size: 4303 843 573 447 331 267 107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4321 5082 6110 1540 Size: 275 186 2320 761 1028 2301 Fragments arranged by size: 2320 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2340 2784 3361 4932 5017 6088 2121 Size: 219 444 577 1571 85 1071 2904 Fragments arranged by size: 2904 1571 1071 577 444 219 85 BsmI GAATG_Cn' Cuts at: 2329 3384 4382 4475 2329 Size: 1055 998 93 4725 Fragments arranged by size: 4725 1055 998 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2057 2057 Size: 6871 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2121 2340 2784 3361 3720 4190 Size: 399 223 108 219 444 577 359 470 Cuts at: 4190 4932 5017 6088 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 577 470 444 399 359 223 219 108 85 BspEI T'CCGG_A Cuts at: 4002 6534 4002 Size: 2532 4339 BspHI T'CATG_A Cuts at: 3396 3525 4760 6490 3396 Size: 129 1235 1730 3777 Fragments arranged by size: 3777 1730 1235 129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4226 844 Size: 3382 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4758 1394 Size: 429 2935 3507 Fragments arranged by size: 3507 2935 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5366 5540 65 Size: 1183 4118 174 1396 Fragments arranged by size: 4118 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5515 5702 1700 Size: 110 3705 187 2869 Fragments arranged by size: 3705 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2870 96 Size: 2774 4097 BssHII G'CGCG_C Cuts at: 1925 2148 1925 Size: 223 6648 BssSI C'ACGA_G Cuts at: 4931 5943 4931 Size: 1012 5859 BstAPI GCAn_nnn'nTGC Cuts at: 794 3271 3545 794 Size: 2477 274 4120 Fragments arranged by size: 4120 2477 274 BstBI TT'CG_AA Cuts at: 2362 5675 2362 Size: 3313 3558 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6871 BstYI r'GATC_y Cuts at: 1972 3678 4201 4568 4953 4970 6422 6508 6520 Size: 1706 523 367 385 17 1452 86 12 Cuts at: 6520 6866 1972 Size: 346 1977 Fragments arranged by size: 1977 1706 1452 523 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2285 3621 2285 Size: 1336 5535 BtgI C'CryG_G Cuts at: 513 1065 2004 2641 2805 2901 3093 513 Size: 552 939 637 164 96 192 4291 Fragments arranged by size: 4291 939 637 552 192 164 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6132 Fragments arranged by size: 6132 683 56 BtsI GCAGTG_nn' Cuts at: 3775 4387 5181 5201 3775 Size: 612 794 20 5445 Fragments arranged by size: 5445 794 612 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6871 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6836 DraI TTT'AAA Cuts at: 2206 4175 2206 Size: 1969 4902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2071 5878 809 Size: 507 755 3807 1802 Fragments arranged by size: 3807 1802 755 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2655 3100 4318 Size: 54 1425 50 275 18 825 445 1218 Cuts at: 4318 5207 6836 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1218 889 825 445 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4318 1537 Size: 275 2506 4090 Fragments arranged by size: 4090 2506 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2630 2993 3060 3758 3818 4801 1353 Size: 1277 363 67 698 60 983 3423 Fragments arranged by size: 3423 1277 983 698 363 67 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2992 5458 5832 5978 387 Size: 1064 138 1403 2466 374 146 1280 Fragments arranged by size: 2466 1403 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2119 2119 Size: 6871 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2468 3157 3241 3383 3461 3853 Size: 393 519 317 689 84 142 78 392 Cuts at: 3853 3928 4934 5531 6318 6586 1239 Size: 75 1006 597 787 268 1524 Fragments arranged by size: 1524 1006 787 689 597 519 393 392 317 268 142 84 78 75 EcoO109I rG'GnC_Cy Cuts at: 3801 3801 Size: 6871 EcoRI G'AATT_C Cuts at: 4180 4558 4180 Size: 378 6493 EcoRV GAT'ATC Cuts at: 3322 3322 Size: 6871 FspI TGC'GCA Cuts at: 3153 5377 3153 Size: 2224 4647 HaeII r_GCGC'y Cuts at: 1678 2021 6018 1678 Size: 343 3997 2531 Fragments arranged by size: 3997 2531 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2779 2811 2906 Size: 32 135 32 548 32 627 32 95 Cuts at: 2906 2938 5512 5544 5586 5618 1373 Size: 32 2574 32 42 32 2626 Fragments arranged by size: 2626 2574 627 548 135 95 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2688 3253 3831 4215 4461 669 Size: 988 281 750 565 578 384 246 3079 Fragments arranged by size: 3079 988 750 578 565 384 281 246 HindIII A'AGCT_T Cuts at: 748 3054 748 Size: 2306 4565 HpaI GTT'AAC Cuts at: 2688 4461 2688 Size: 1773 5098 KpnI G_GTAC'C Cuts at: 2909 4196 2909 Size: 1287 5584 MfeI C'AATT_G Cuts at: 4470 4470 Size: 6871 MluI A'CGCG_T Cuts at: 6747 6747 Size: 6871 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3042 3138 4092 5985 6169 1305 Size: 1737 96 954 1893 184 2007 Fragments arranged by size: 2007 1893 1737 954 184 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 2657 10 Size: 54 1425 343 825 4224 Fragments arranged by size: 4224 1425 825 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2526 4829 5188 5347 518 Size: 648 1360 2303 359 159 2042 Fragments arranged by size: 2303 2042 1360 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3520 3745 4287 4964 6112 6357 1606 Size: 1914 225 542 677 1148 245 2120 Fragments arranged by size: 2120 1914 1148 677 542 245 225 NcoI C'CATG_G Cuts at: 513 1065 2004 2641 2805 2901 3093 513 Size: 552 939 637 164 96 192 4291 Fragments arranged by size: 4291 939 637 552 192 164 96 NdeI CA'TA_TG Cuts at: 387 387 Size: 6871 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6871 NotI GC'GGCC_GC Cuts at: 4318 4318 Size: 6871 NsiI A_TGCA'T Cuts at: 3194 3194 Size: 6871 NspI r_CATG'y Cuts at: 1132 1229 4229 5774 1132 Size: 97 3000 1545 2229 Fragments arranged by size: 3000 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6871 PciI A'CATG_T Cuts at: 5770 5770 Size: 6871 PflMI CCAn_nnn'nTGG Cuts at: 1108 3202 1108 Size: 2094 4777 PfoI T'CCnGG_A Cuts at: 3385 3385 Size: 6871 PmeI GTTT'AAAC Cuts at: 4175 4175 Size: 6871 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4940 4972 6453 6485 4940 Size: 32 1481 32 5326 Fragments arranged by size: 5326 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6871 PsiI TTA'TAA Cuts at: 4441 4441 Size: 6871 PspXI vC'TCGA_Gb Cuts at: 2021 2021 Size: 6871 PstI C_TGCA'G Cuts at: 830 3794 4223 830 Size: 2964 429 3478 Fragments arranged by size: 3478 2964 429 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6871 PvuII CAG'CTG Cuts at: 3520 3745 3520 Size: 225 6646 SacI G_AGCT'C Cuts at: 2121 2121 Size: 6871 SalI G'TCGA_C Cuts at: 1936 4213 1936 Size: 2277 4594 SapI GCTCTTCn'nnn_ Cuts at: 3758 3818 3758 Size: 60 6811 SbfI CC_TGCA'GG Cuts at: 4223 4223 Size: 6871 ScaI AGT'ACT Cuts at: 5119 6831 5119 Size: 1712 5159 SexAI A'CCwGG_T Cuts at: 2508 2508 Size: 6871 SfcI C'TryA_G Cuts at: 826 1045 3790 4219 5354 6035 6226 826 Size: 219 2745 429 1135 681 191 1471 Fragments arranged by size: 2745 1471 1135 681 429 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6871 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6871 SmaI CCC'GGG Cuts at: 1872 4198 1872 Size: 2326 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 2069 2153 2777 4975 5876 Size: 197 926 78 48 84 624 2198 901 Cuts at: 5876 6138 820 Size: 262 1553 Fragments arranged by size: 2198 1553 926 901 624 262 197 84 78 48 SnaBI TAC'GTA Cuts at: 493 4662 493 Size: 4169 2702 SpeI A'CTAG_T Cuts at: 152 3353 152 Size: 3201 3670 SphI G_CATG'C Cuts at: 1132 4229 1132 Size: 3097 3774 SspI AAT'ATT Cuts at: 5 52 4795 5 Size: 47 4743 2081 Fragments arranged by size: 4743 2081 47 StuI AGG'CCT Cuts at: 1517 3619 1517 Size: 2102 4769 StyI C'CwwG_G Cuts at: 513 1065 2004 2349 2385 2641 2658 2805 2901 Size: 552 939 345 36 256 17 147 96 Cuts at: 2901 3093 3586 4310 513 Size: 192 493 724 3074 Fragments arranged by size: 3074 939 724 552 493 345 256 192 147 96 36 17 SwaI ATTT'AAAT Cuts at: 2206 2206 Size: 6871 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2845 4104 4898 4915 5068 5253 5698 Size: 1255 1265 1259 794 17 153 185 445 Cuts at: 5698 325 Size: 1498 Fragments arranged by size: 1498 1265 1259 1255 794 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2870 5117 6829 Size: 274 80 33 51 1443 893 2247 1712 Cuts at: 6829 96 Size: 138 Fragments arranged by size: 2247 1712 1443 893 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6831 6863 6831 Size: 32 6839 Tth111I GACn'n_nGTC Cuts at: 1316 2510 1316 Size: 1194 5677 XbaI T'CTAG_A Cuts at: 4207 4207 Size: 6871 XhoI C'TCGA_G Cuts at: 1943 2021 1943 Size: 78 6793 XmaI C'CCGG_G Cuts at: 1870 4196 1870 Size: 2326 4545 XmnI GAAnn'nnTTC Cuts at: 5000 5000 Size: 6871 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4679 276 Size: 53 83 186 936 3145 2468 Fragments arranged by size: 3145 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BsiWI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI XcmI