MRGPRE ORF clone pF1KE0803

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Flexi ORF Clone pF1KE0803

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Clone info.

Species

human

Clone Name

pF1KE0803 Sequence (with vector) Restriction Map

Product ID

FXC11277

HaloTag Clone PID

FHC11277

ORF Sequence

DNA sequence (936 bp)
Protein sequence (312 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529239]

Gene

KIEE0803

Symbol

MRGPRE

Alias : GPR167, MGRF, MRGE

Description

MAS-related GPR, member E

Original Clone ID

cp01203

Features of the cloned ORF sequence

Description

Length: 936 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 312 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS41603.2 4.7e-135 100.0 MRGPRE CCDS7830.1 1.3e-37 39.1 MRGPRX3 CCDS7846.1 4.2e-37 39.4 MRGPRX1 CCDS7847.1 1.4e-36 39.2 MRGPRX2 CCDS31625.1 4.1e-36 38.6 MRGPRD The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 26 50 PR00237 GPCR IPR000276 58 79 PR00237 GPCR IPR000276 103 125 PR00237 GPCR IPR000276 215 239 PR00237 GPCR IPR000276 251 277 PR00237 GPCR HMMPfam IPR000276 41 200 PF00001 GPCR ProfileScan IPR017452 41 269 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 24 NLIILSLTEGLGLGGLLGNGAVL 46 SECONDARY 23 2 60 IYLLDVACADLIFLGCHMVAIVP 82 PRIMARY 23 3 103 RFFCYIVGLSLLAAVSVEQCLAA 125 PRIMARY 23 4 142 CVCALTWALCLLLHLLLSGACTQ 164 PRIMARY 23 5 178 WLVAAVLLALLCCTMCGASLMLL 200 PRIMARY 23 6 214 GFPGLILLTVLLFLFCGLPFGIY 236 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001034254 312 607232 100.0 100.0 XP_011518184 322 607229 39.1 92.6 NP_473372 322 607229 39.1 92.6 NP_671732 322 607227 39.4 90.4 NP_001290544 330 607228 39.2 92.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE0803 Download

>KIEE0803  936 bp
ATGATGGAGCCCAGAGAAGCTGGACAGCACGTGGGGGCCGCCAACGGCGCCCAGGAGGAT
GTGGCCTTCAACCTCATCATCCTGTCCCTCACCGAGGGGCTCGGCCTCGGTGGGCTGCTG
GGGAATGGGGCAGTCCTCTGGCTGCTCAGCTCCAATGTCTACAGAAACCCCTTCGCCATC
TACCTCCTGGACGTGGCCTGCGCGGATCTCATCTTCCTTGGCTGCCACATGGTGGCCATC
GTCCCCGACTTGCTGCAAGGCCGGCTGGACTTCCCGGGCTTCGTGCAGACCAGCCTGGCA
ACGCTGCGCTTCTTCTGCTACATCGTGGGCCTGAGTCTCCTGGCGGCCGTCAGCGTGGAG
CAGTGCCTGGCCGCCCTCTTCCCAGCCTGGTACTCGTGCCGCCGCCCACGCCACCTGACC
ACCTGTGTGTGCGCCCTCACCTGGGCCCTCTGCCTGCTGCTGCACCTGCTGCTCAGCGGC
GCCTGCACCCAGTTCTTCGGGGAGCCCAGCCGCCACTTGTGCCGGACGCTGTGGCTGGTG
GCAGCGGTGCTGCTGGCTCTGCTGTGTTGCACCATGTGTGGGGCCAGCCTTATGCTGCTG
CTGCGGGTGGAGCGAGGCCCCCAGCGGCCCCCACCCCGGGGCTTCCCTGGGCTCATCCTC
CTCACCGTCCTCCTCTTCCTCTTCTGCGGCCTGCCCTTCGGCATCTACTGGCTGTCCCGG
AACCTGCTCTGGTACATCCCCCACTACTTCTACCACTTCAGCTTCCTCATGGCCGCCGTG
CACTGCGCGGCCAAGCCCGTCGTCTACTTCTGCCTGGGCAGTGCCCAGGGCCGCAGGCTG
CCCCTCCGGCTGGTCCTCCAGCGAGCGCTGGGAGACGAGGCTGAGCTGGGGGCCGTCAGG
GAGACCTCCCGCCGGGGCCTGGTGGACATAGCAGCC

ORF Protein

Cloned ORF protein sequence for pF1KE0803 Download

>KIEE0803  312 aa
MMEPREAGQHVGAANGAQEDVAFNLIILSLTEGLGLGGLLGNGAVLWLLSSNVYRNPFAI
YLLDVACADLIFLGCHMVAIVPDLLQGRLDFPGFVQTSLATLRFFCYIVGLSLLAAVSVE
QCLAALFPAWYSCRRPRHLTTCVCALTWALCLLLHLLLSGACTQFFGEPSRHLCRTLWLV
AAVLLALLCCTMCGASLMLLLRVERGPQRPPPRGFPGLILLTVLLFLFCGLPFGIYWLSR
NLLWYIPHYFYHFSFLMAAVHCAAKPVVYFCLGSAQGRRLPLRLVLQRALGDEAELGAVR
ETSRRGLVDIAA

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE0803 Download

>pF1KE0803  4049 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGATGGAGCCC
AGAGAAGCTGGACAGCACGTGGGGGCCGCCAACGGCGCCCAGGAGGATGTGGCCTTCAAC
CTCATCATCCTGTCCCTCACCGAGGGGCTCGGCCTCGGTGGGCTGCTGGGGAATGGGGCA
GTCCTCTGGCTGCTCAGCTCCAATGTCTACAGAAACCCCTTCGCCATCTACCTCCTGGAC
GTGGCCTGCGCGGATCTCATCTTCCTTGGCTGCCACATGGTGGCCATCGTCCCCGACTTG
CTGCAAGGCCGGCTGGACTTCCCGGGCTTCGTGCAGACCAGCCTGGCAACGCTGCGCTTC
TTCTGCTACATCGTGGGCCTGAGTCTCCTGGCGGCCGTCAGCGTGGAGCAGTGCCTGGCC
GCCCTCTTCCCAGCCTGGTACTCGTGCCGCCGCCCACGCCACCTGACCACCTGTGTGTGC
GCCCTCACCTGGGCCCTCTGCCTGCTGCTGCACCTGCTGCTCAGCGGCGCCTGCACCCAG
TTCTTCGGGGAGCCCAGCCGCCACTTGTGCCGGACGCTGTGGCTGGTGGCAGCGGTGCTG
CTGGCTCTGCTGTGTTGCACCATGTGTGGGGCCAGCCTTATGCTGCTGCTGCGGGTGGAG
CGAGGCCCCCAGCGGCCCCCACCCCGGGGCTTCCCTGGGCTCATCCTCCTCACCGTCCTC
CTCTTCCTCTTCTGCGGCCTGCCCTTCGGCATCTACTGGCTGTCCCGGAACCTGCTCTGG
TACATCCCCCACTACTTCTACCACTTCAGCTTCCTCATGGCCGCCGTGCACTGCGCGGCC
AAGCCCGTCGTCTACTTCTGCCTGGGCAGTGCCCAGGGCCGCAGGCTGCCCCTCCGGCTG
GTCCTCCAGCGAGCGCTGGGAGACGAGGCTGAGCTGGGGGCCGTCAGGGAGACCTCCCGC
CGGGGCCTGGTGGACATAGCAGCCGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCT
CTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC
TGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACG
GGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAA
AACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGC
GCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCG
TTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTG
CTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGG
AAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGA
TCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTG
CACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAG
ACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTT
TTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTA
TCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCG
GGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTT
GCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGAT
CCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGG
ATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCA
GCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACT
CATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATC
GACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGAT
ATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCC
GCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGA
CTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGC
GGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGG
CCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCG
CCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGG
ACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGAC
CCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCA
TAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGT
GCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTC
CAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAG
AGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACAC
TAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT
TGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAA
GCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGG
GTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAA
AAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCC
CTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAAT
GTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATT
GCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTG
AACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCC
TCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCG
AGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAA
TTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAA
ACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGC
ATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGT
CGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGC
AACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGC
AGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCAT