Restriction map for pF1KE0803
 (Linear) (Six-base) MAPSORT of: KIEE0803.seq  Check: 1452  from: 1  to: 936



KIEE0803.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     473     936 
   Size:        473     463
 
AccI GT'mk_AC

Cuts at:      0     158     803     936 
   Size:        158     645     133

  Fragments arranged by size: 

                 645     158     133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     741     936 
   Size:        741     195
 
AfeI AGC'GCT

Cuts at:      0     866     936 
   Size:        866      70
 
AleI CACnn'nnGTG

Cuts at:      0     424     936 
   Size:        424     512
 
AlwNI CAG_nnn'CTG

Cuts at:      0     366     936 
   Size:        366     570
 
ApaI G_GGCC'C

Cuts at:      0     447     936 
   Size:        447     489
 
ApaLI G'TGCA_C

Cuts at:      0     778     936 
   Size:        778     158
 
AvaI C'yCGr_G

Cuts at:      0     273     635     936 
   Size:        273     362     301

  Fragments arranged by size: 

                 362     301     273
 
BanI G'GyrC_C

Cuts at:      0      46     478     936 
   Size:         46     432     458

  Fragments arranged by size: 

                 458     432      46
 
BanII G_rGCy'C

Cuts at:      0      11     101     447     506     653     936 
   Size:         11      90     346      59     147     283

  Fragments arranged by size: 

                 346     283     147      90      59      11
 
BbeI G_GCGC'C

Cuts at:      0      50     482     936 
   Size:         50     432     454

  Fragments arranged by size: 

                 454     432      50
 
BglI GCCn_nnn'nGGC

Cuts at:      0     846     936 
   Size:        846      90
 
BlpI GC'TnA_GC

Cuts at:      0     145     472     881     936 
   Size:        145     327     409      55

  Fragments arranged by size: 

                 409     327     145      55
 
Bme1580I G_kGCm'C

Cuts at:      0     447     782     825     936 
   Size:        447     335      43     111

  Fragments arranged by size: 

                 447     335     111      43
 
BmgBI CAC'GTC

Cuts at:      0     192     936 
   Size:        192     744
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     483     936 
   Size:        483     453
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841     936 
   Size:        841      95
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     895     936 
   Size:        895      41
 
BsaAI yAC'GTr

Cuts at:      0      30     936 
   Size:         30     906
 
BsaHI Gr'CG_yC

Cuts at:      0      47     479     936 
   Size:         47     432     457

  Fragments arranged by size: 

                 457     432      47
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     649     661     936 
   Size:        649      12     275

  Fragments arranged by size: 

                 649     275      12
 
BseYI C'CCAG_C

Cuts at:      0     117     381     505     620     867     885     936 
   Size:        117     264     124     115     247      18      51

  Fragments arranged by size: 

                 264     247     124     117     115      51      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     445     468     936 
   Size:        304     141      23     468

  Fragments arranged by size: 

                 468     304     141      23
 
BsiEI CG_ry'CG

Cuts at:      0     347     936 
   Size:        347     589
 
BsiHKAI G_wGCw'C

Cuts at:      0     782     936 
   Size:        782     154
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     866     936 
   Size:        866      70
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     101     447     506     653     782     825     936 
   Size:         11      90     346      59     147     129      43     111

  Fragments arranged by size: 

                 346     147     129     111      90      59      43      11
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     473     731     936 
   Size:        473     258     205

  Fragments arranged by size: 

                 473     258     205
 
BsrFI r'CCGG_y

Cuts at:      0     260     936 
   Size:        260     676
 
BssSI C'ACGA_G

Cuts at:      0     393     936 
   Size:        393     543
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     468     936 
   Size:        468     468
 
BstYI r'GATC_y

Cuts at:      0     204     936 
   Size:        204     732
 
BtsI GCAGTG_nn'

Cuts at:      0     367     780     825     936 
   Size:        367     413      45     111

  Fragments arranged by size: 

                 413     367     111      45
 
DraIII CAC_nnn'GTG

Cuts at:      0     231     575     936 
   Size:        231     344     361

  Fragments arranged by size: 

                 361     344     231
 
EaeI y'GGCC_r

Cuts at:      0     233     344     368     770     788     936 
   Size:        233     111      24     402      18     148

  Fragments arranged by size: 

                 402     233     148     111      24      18
 
EagI C'GGCC_G

Cuts at:      0     344     936 
   Size:        344     592
 
EarI CTCTTCn'nnn_

Cuts at:      0     382     679     685     936 
   Size:        382     297       6     251

  Fragments arranged by size: 

                 382     297     251       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     741     841     936 
   Size:        741     100      95

  Fragments arranged by size: 

                 741     100      95
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      91     936 
   Size:         91     845
 
EcoO109I rG'GnC_Cy

Cuts at:      0     444     616     915     936 
   Size:        444     172     299      21

  Fragments arranged by size: 

                 444     299     172      21
 
HaeII r_GCGC'y

Cuts at:      0      50     482     868     936 
   Size:         50     432     386      68

  Fragments arranged by size: 

                 432     386      68      50
 
KasI G'GCGC_C

Cuts at:      0      46     478     936 
   Size:         46     432     458

  Fragments arranged by size: 

                 458     432      46
 
MscI TGG'CCA

Cuts at:      0     235     936 
   Size:        235     701
 
MslI CAynn'nnrTG

Cuts at:      0     424     936 
   Size:        424     512
 
MspA1I CmG'CkG

Cuts at:      0     476     544     624     936 
   Size:        476      68      80     312

  Fragments arranged by size: 

                 476     312      80      68
 
NaeI GCC'GGC

Cuts at:      0     262     936 
   Size:        262     674
 
NarI GG'CG_CC

Cuts at:      0      47     479     936 
   Size:         47     432     457

  Fragments arranged by size: 

                 457     432      47
 
NgoMIV G'CCGG_C

Cuts at:      0     260     936 
   Size:        260     676
 
PasI CC'CwG_GG

Cuts at:      0     646     825     936 
   Size:        646     179     111

  Fragments arranged by size: 

                 646     179     111
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     231     936 
   Size:        231     705
 
PfoI T'CCnGG_A

Cuts at:      0     185     715     936 
   Size:        185     530     221

  Fragments arranged by size: 

                 530     221     185
 
PmlI CAC'GTG

Cuts at:      0      30     936 
   Size:         30     906
 
PspOMI G'GGCC_C

Cuts at:      0     443     936 
   Size:        443     493
 
SfcI C'TryA_G

Cuts at:      0     159     936 
   Size:        159     777
 
SfoI GGC'GCC

Cuts at:      0      48     480     936 
   Size:         48     432     456

  Fragments arranged by size: 

                 456     432      48
 
SmaI CCC'GGG

Cuts at:      0     275     637     936 
   Size:        275     362     299

  Fragments arranged by size: 

                 362     299     275
 
StyI C'CwwG_G

Cuts at:      0     216     936 
   Size:        216     720
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     502     936 
   Size:        502     434
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      14      46     936 
   Size:         14      32     890

  Fragments arranged by size: 

                 890      32      14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     183     936 
   Size:        183     753
 
XmaI C'CCGG_G

Cuts at:      0     273     635     936 
   Size:        273     362     301

  Fragments arranged by size: 

                 362     301     273
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AleI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BpmI     BsaI    BsaAI    BsaHI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI    BssSI   BstAPI
    BstYI     BtsI   DraIII     EaeI     EagI     EarI  Eco57MI    EcoNI
 EcoO109I    HaeII     KasI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     PasI    PflMI     PfoI     PmlI   PspOMI     SfcI     SfoI
     SmaI     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaWI    BsaXI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     DrdI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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