FLJ32756 ORF clone pF1KE0526

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Flexi ORF Clone pF1KE0526

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Clone info.

Species

human

Clone Name

pF1KE0526 Sequence (with vector) Restriction Map

Product ID

FXC05212

HaloTag Clone PID

FHC05212

ORF Sequence

DNA sequence (531 bp)
Protein sequence (177 aa)

Linker Type

Flexi type

more Linker info

The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site (GTTTAAAC) is tagged at the 3' end of the ORF instead of the stop codon. The resultant sequence encodes Val-stop in place of stop. By cutting the sequence using Pme I, the stop codon arising in the Pme I site can be omitted.

Vector Type

pF1K Vector sequence Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528137]

Gene

KIEE0526

Symbol

FLJ32756

Description

hypothetical LOC642757

Original Clone ID

oc01226

Features of the cloned ORF sequence

Description

Length: 531 bp

Restriction map A Restriction map B

Genomic Structure

Features of the protein sequence

Description

Length: 177 aa

Result of homology search against CCDS protein database ( FASTA output, Multiple alignment)

no hit
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE0526 Download

>KIEE0526  531 bp
ATGAGCCACTCCCGAAGAGCTGCTCCTACTCAGGACCAATGCCACACTCCAGGATTCCCC
ACCTCCCGTGAAACCAGTGGGAGCATATGGCAGGCCAGGATCTGTGGATCTTTACAGGCT
CTGGACACATGGCGAACACACATCCCCAGAAAGAGCCCAGCTCCCACTCAGGCATCACAG
ATCTGTCTGCTGTTGCCTGAAAGCCCCTGGAGAAATCCCACACCCCGGGGATTTCTTAAA
CCTTTGATTAACTGGGATGCTATCCTGTACTTCAAAGAAAAGAGGAATATCCAAGTAACA
ACACAAGCTCATCCCCAGAACCAGGCCAGCTGCAGCTCTCAGGAGGTAGCCACACCCGGG
CTAGTGCCACAGGCAGCTGCACCAAAGGTGTATGAGCGGTCACATGATAACCTGAACGCT
GAGGCCCAAGGACTGGCAGGTGCTCAGGTGTCGAAACCACAGAATCCCATTACCAGGCTC
TGTTCTCTGAAGGAACAGTCAATCTTGAAGATCTTCACTAAGCAGAGCATC

ORF Protein

Cloned ORF protein sequence for pF1KE0526 Download

>KIEE0526  177 aa
MSHSRRAAPTQDQCHTPGFPTSRETSGSIWQARICGSLQALDTWRTHIPRKSPAPTQASQ
ICLLLPESPWRNPTPRGFLKPLINWDAILYFKEKRNIQVTTQAHPQNQASCSSQEVATPG
LVPQAAAPKVYERSHDNLNAEAQGLAGAQVSKPQNPITRLCSLKEQSILKIFTKQSI

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE0526 Download

>pF1KE0526  3644 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGAGCCACTCC
CGAAGAGCTGCTCCTACTCAGGACCAATGCCACACTCCAGGATTCCCCACCTCCCGTGAA
ACCAGTGGGAGCATATGGCAGGCCAGGATCTGTGGATCTTTACAGGCTCTGGACACATGG
CGAACACACATCCCCAGAAAGAGCCCAGCTCCCACTCAGGCATCACAGATCTGTCTGCTG
TTGCCTGAAAGCCCCTGGAGAAATCCCACACCCCGGGGATTTCTTAAACCTTTGATTAAC
TGGGATGCTATCCTGTACTTCAAAGAAAAGAGGAATATCCAAGTAACAACACAAGCTCAT
CCCCAGAACCAGGCCAGCTGCAGCTCTCAGGAGGTAGCCACACCCGGGCTAGTGCCACAG
GCAGCTGCACCAAAGGTGTATGAGCGGTCACATGATAACCTGAACGCTGAGGCCCAAGGA
CTGGCAGGTGCTCAGGTGTCGAAACCACAGAATCCCATTACCAGGCTCTGTTCTCTGAAG
GAACAGTCAATCTTGAAGATCTTCACTAAGCAGAGCATCGTTTAAACGAATTCGAGCTCG
GTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAA
AGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCT
TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTC
GCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTAC
CTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCAT
CCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCC
CTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCC
TCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATC
TGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTT
GAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTAT
GACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAG
GGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGAC
GAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGAC
GTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTC
CTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGG
CTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAG
CGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCAT
CAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAG
GATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGC
TTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCG
TTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTG
CTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAG
TTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATC
AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAA
CATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTT
TTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG
GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCG
CTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAG
CGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTC
CAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAA
CTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG
TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCC
TAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC
CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGG
TTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTT
GATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGT
CATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAAC
GCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGA
TTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAAT
TTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCT
GCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCC
ACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTC
CGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGG
TCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCC
GAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGT
AGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTT
TTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATT
TGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCA
GGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTC
TTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT