(Linear) (Six-base) MAPSORT of: KIEE0526.seq  Check: 2010  from: 1  to: 531



KIEE0526.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     427     531 
   Size:        427     104
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     531 
   Size:        508      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     101     120     479     531 
   Size:        101      19     359      52

  Fragments arranged by size: 

                 359     101      52      19
 
AvaI C'yCGr_G

Cuts at:      0     224     355     531 
   Size:        224     131     176

  Fragments arranged by size: 

                 224     176     131
 
BanII G_rGCy'C

Cuts at:      0     157     531 
   Size:        157     374
 
BbvCI CC'TCA_GC

Cuts at:      0     419     531 
   Size:        419     112
 
BglII A'GATC_T

Cuts at:      0     179     509     531 
   Size:        179     330      22

  Fragments arranged by size: 

                 330     179      22
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     261     531 
   Size:        261     270
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     176     531 
   Size:        176     355
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     228     531 
   Size:         32     196     303

  Fragments arranged by size: 

                 303     196      32
 
Bpu10I CC'TnA_GC

Cuts at:      0     419     443     531 
   Size:        419      24      88

  Fragments arranged by size: 

                 419      88      24
 
BseYI C'CCAG_C

Cuts at:      0     156     531 
   Size:        156     375
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     531 
   Size:        362     169
 
BsiHKAI G_wGCw'C

Cuts at:      0     444     531 
   Size:        444      87
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     444     531 
   Size:        157     287      87

  Fragments arranged by size: 

                 287     157      87
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     427     531 
   Size:        427     104
 
BsrBI CCG'CTC

Cuts at:      0     396     531 
   Size:        396     135
 
BstYI r'GATC_y

Cuts at:      0      98     106     179     509     531 
   Size:         98       8      73     330      22

  Fragments arranged by size: 

                 330      98      73      22       8
 
EarI CTCTTCn'nnn_

Cuts at:      0       9     531 
   Size:          9     522
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     228     508     531 
   Size:         32     196     280      23

  Fragments arranged by size: 

                 280     196      32      23
 
MspA1I CmG'CkG

Cuts at:      0     329     376     531 
   Size:        329      47     155

  Fragments arranged by size: 

                 329     155      47
 
NdeI CA'TA_TG

Cuts at:      0      85     531 
   Size:         85     446
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     432     531 
   Size:        432      99
 
PfoI T'CCnGG_A

Cuts at:      0      48     531 
   Size:         48     483
 
PstI C_TGCA'G

Cuts at:      0     334     531 
   Size:        334     197
 
PvuII CAG'CTG

Cuts at:      0     329     376     531 
   Size:        329      47     155

  Fragments arranged by size: 

                 329     155      47
 
SapI GCTCTTCn'nnn_

Cuts at:      0       9     531 
   Size:          9     522
 
SfcI C'TryA_G

Cuts at:      0     330     531 
   Size:        330     201
 
SmaI CCC'GGG

Cuts at:      0     226     357     531 
   Size:        226     131     174

  Fragments arranged by size: 

                 226     174     131
 
StyI C'CwwG_G

Cuts at:      0     426     531 
   Size:        426     105
 
TatI w'GTAC_w

Cuts at:      0     266     531 
   Size:        266     265
 
XmaI C'CCGG_G

Cuts at:      0     224     355     531 
   Size:        224     131     176

  Fragments arranged by size: 

                 224     176     131
 
XmnI GAAnn'nnTTC

Cuts at:      0     511     531 
   Size:        511      20
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     AvaI    BanII    BbvCI    BglII     BmrI
     BplI     BpmI   Bpu10I    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BstYI     EarI  Eco57MI   MspA1I     NdeI    PflMI     PfoI
     PstI    PvuII     SapI     SfcI     SmaI     StyI     TatI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI