SASH3 ORF clone FHC11214

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11214

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Clone info.

Species

human

Clone Name

pFN21AE0646 Sequence (with vector) Restriction Map

Product ID

FHC11214

Flexi Clone PID

FXC11214

ORF Sequence

DNA sequence (1140 bp)
Protein sequence (380 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528892]

Gene

KIEE0646

Symbol

SASH3

Alias : 753P9, CXorf9, HACS2, SH3D6C, SLY

Description

SAM and SH3 domain containing 3

Original Clone ID

cs02146

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1140 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 380 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14614.1 8.5e-117 100.0 SASH3 CCDS5212.1 1.8e-34 45.6 SASH1 CCDS42906.1 2.9e-34 45.7 SAMSN1 CCDS58786.1 5.8e-34 44.7 SAMSN1 CCDS74774.1 3.9e-31 47.5 SAMSN1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR021090 19 174 PF12485 SAM/SH3 domain-containing IPR011511 177 229 PF07653 Variant SH3 IPR011510 253 313 PF07647 Sterile alpha motif HMMSmart IPR001452 176 233 SM00326 Src homology-3 domain IPR001660 249 316 SM00454 Sterile alpha motif domain ProfileScan IPR001660 252 316 PS50105 Sterile alpha motif domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_061863 380 300441 100.0 100.0 XP_006724826 430 300441 87.9 95.3 NP_001333436 1008 607955 45.6 88.9 NP_001333435 1123 607955 45.6 88.9 NP_001333434 1202 607955 45.6 88.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0646 Download

>KIEE0646  1140 bp
ATGCTGCGCCGCAAGCCCTCCAATGCCAGTGAGAAGGAGCCCACTCAGAAGAAAAAGCTC
TCCCTTCAGCGCTCCAGCAGCTTCAAGGATTTTGCCAAATCCAAACCCAGCTCCCCCGTG
GTGAGCGAGAAGGAGTTTAATCTGGATGATAACATTCCAGAAGATGACTCAGGTGTCCCC
ACCCCAGAAGATGCTGGGAAGAGTGGCAAAAAGCTGGGGAAGAAGTGGAGGGCAGTGATT
TCCCGAACCATGAACAGGAAGATGGGCAAGATGATGGTGAAGGCCCTGTCAGAAGAGATG
GCAGACACTCTGGAGGAGGGCTCTGCCTCCCCGACATCTCCAGACTACAGCCTGGACAGC
CCTGGCCCTGAGAAGATGGCGCTGGCCTTTTCTGAGCAAGAGGAGCATGAACTTCCGGTG
CTCAGCCGCCAGGCATCAACAGGCAGTGAGCTCTGCAGCCCCAGCCCAGGTTCTGGCAGC
TTCGGGGAGGAACCACCTGCCCCCCAGTACACAGGGCCTTTCTGTGGCCGGGCACGAGTC
CACACCGACTTCACTCCCAGCCCCTATGACCACGACTCGCTGAAACTGCAGAAAGGAGAT
GTGATCCAGATCATTGAAAAGCCACCTGTGGGCACGTGGCTGGGCCTACTCAATGGCAAG
GTGGGCTCTTTCAAATTCATCTATGTGGATGTGCTGCCCGAGGAGGCCGTGGGGCATGCC
CGCCCCAGCCGCCGACAGAGCAAGGGCAAGAGGCCCAAGCCTAAGACCCTGCATGAGCTG
CTGGAGCGCATCGGCCTGGAGGAGCACACATCCACCCTCCTGCTCAATGGCTACCAGACA
CTGGAAGACTTCAAAGAGCTGCGAGAAACACACCTCAATGAGCTGAACATCATGGATCCA
CAGCACCGGGCCAAGCTGCTCACGGCCGCCGAGCTGCTGCTGGACTATGACACTGGCAGT
GAGGAGGCTGAAGAGGGCGCCGAGAGCAGCCAGGAGCCAGTGGCACACACAGTGTCGGAA
CCCAAGGTGGACATCCCGCGCGACTCAGGCTGCTTTGAGGGCTCGGAGAGCGGGCGCGAT
GACGCAGAGCTGGCAGGCACTGAGGAGCAGCTGCAAGGCCTCTCCCTGGCCGGGGCACCT

ORF Protein

Cloned ORF protein sequence for pFN21AE0646 Download

>KIEE0646  380 aa
MLRRKPSNASEKEPTQKKKLSLQRSSSFKDFAKSKPSSPVVSEKEFNLDDNIPEDDSGVP
TPEDAGKSGKKLGKKWRAVISRTMNRKMGKMMVKALSEEMADTLEEGSASPTSPDYSLDS
PGPEKMALAFSEQEEHELPVLSRQASTGSELCSPSPGSGSFGEEPPAPQYTGPFCGRARV
HTDFTPSPYDHDSLKLQKGDVIQIIEKPPVGTWLGLLNGKVGSFKFIYVDVLPEEAVGHA
RPSRRQSKGKRPKPKTLHELLERIGLEEHTSTLLLNGYQTLEDFKELRETHLNELNIMDP
QHRAKLLTAAELLLDYDTGSEEAEEGAESSQEPVAHTVSEPKVDIPRDSGCFEGSESGRD
DAELAGTEEQLQGLSLAGAP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0646 Download

>pFN21AE0646  5845 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGCGCCGCAAGCCCTCCAATGCCAGTGAGAA
GGAGCCCACTCAGAAGAAAAAGCTCTCCCTTCAGCGCTCCAGCAGCTTCAAGGATTTTGC
CAAATCCAAACCCAGCTCCCCCGTGGTGAGCGAGAAGGAGTTTAATCTGGATGATAACAT
TCCAGAAGATGACTCAGGTGTCCCCACCCCAGAAGATGCTGGGAAGAGTGGCAAAAAGCT
GGGGAAGAAGTGGAGGGCAGTGATTTCCCGAACCATGAACAGGAAGATGGGCAAGATGAT
GGTGAAGGCCCTGTCAGAAGAGATGGCAGACACTCTGGAGGAGGGCTCTGCCTCCCCGAC
ATCTCCAGACTACAGCCTGGACAGCCCTGGCCCTGAGAAGATGGCGCTGGCCTTTTCTGA
GCAAGAGGAGCATGAACTTCCGGTGCTCAGCCGCCAGGCATCAACAGGCAGTGAGCTCTG
CAGCCCCAGCCCAGGTTCTGGCAGCTTCGGGGAGGAACCACCTGCCCCCCAGTACACAGG
GCCTTTCTGTGGCCGGGCACGAGTCCACACCGACTTCACTCCCAGCCCCTATGACCACGA
CTCGCTGAAACTGCAGAAAGGAGATGTGATCCAGATCATTGAAAAGCCACCTGTGGGCAC
GTGGCTGGGCCTACTCAATGGCAAGGTGGGCTCTTTCAAATTCATCTATGTGGATGTGCT
GCCCGAGGAGGCCGTGGGGCATGCCCGCCCCAGCCGCCGACAGAGCAAGGGCAAGAGGCC
CAAGCCTAAGACCCTGCATGAGCTGCTGGAGCGCATCGGCCTGGAGGAGCACACATCCAC
CCTCCTGCTCAATGGCTACCAGACACTGGAAGACTTCAAAGAGCTGCGAGAAACACACCT
CAATGAGCTGAACATCATGGATCCACAGCACCGGGCCAAGCTGCTCACGGCCGCCGAGCT
GCTGCTGGACTATGACACTGGCAGTGAGGAGGCTGAAGAGGGCGCCGAGAGCAGCCAGGA
GCCAGTGGCACACACAGTGTCGGAACCCAAGGTGGACATCCCGCGCGACTCAGGCTGCTT
TGAGGGCTCGGAGAGCGGGCGCGATGACGCAGAGCTGGCAGGCACTGAGGAGCAGCTGCA
AGGCCTCTCCCTGGCCGGGGCACCTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT