(Circular) (Six-base) MAPSORT of: pFN21AE0646.seq Check: 5381 from: 1 to: 5845 pFN21AE0646 5845 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2509 2509 Size: 5845 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2994 3908 5292 5560 1632 Size: 422 940 914 1384 268 1917 Fragments arranged by size: 1917 1384 940 914 422 268 AfeI AGC'GCT Cuts at: 2075 2075 Size: 5845 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5845 AlwNI CAG_nnn'CTG Cuts at: 2477 2845 3104 3588 5160 2477 Size: 368 259 484 1572 3162 Fragments arranged by size: 3162 1572 484 368 259 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 2678 3154 3380 3532 5641 1493 Size: 1185 476 226 152 2109 1697 Fragments arranged by size: 2109 1697 1185 476 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2702 3170 1870 Size: 73 759 468 4545 Fragments arranged by size: 4545 759 468 73 BamHI G'GATC_C Cuts at: 2899 3175 3542 2899 Size: 276 367 5202 Fragments arranged by size: 5202 367 276 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2981 3139 3166 4621 618 Size: 325 217 627 1194 158 27 1455 1842 Fragments arranged by size: 1842 1455 1194 627 325 217 158 27 BanII G_rGCy'C Cuts at: 1391 2046 2327 2457 2672 3068 3164 1391 Size: 655 281 130 215 396 96 4072 Fragments arranged by size: 4072 655 396 281 215 130 96 BbeI G_GCGC'C Cuts at: 2985 2985 Size: 5845 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2856 928 Size: 971 957 3917 Fragments arranged by size: 3917 971 957 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4036 4070 1903 Size: 34 50 34 2015 34 3678 Fragments arranged by size: 3678 2015 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4456 136 Size: 107 122 71 631 605 2784 1525 Fragments arranged by size: 2784 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 2426 3262 2426 Size: 836 5009 Bme1580I G_kGCm'C Cuts at: 1790 2539 2639 3142 3906 5062 1790 Size: 749 100 503 764 1156 2573 Fragments arranged by size: 2573 1156 764 749 503 100 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2503 3576 4534 Size: 416 53 83 399 24 1086 1073 958 Cuts at: 4534 442 Size: 1753 Fragments arranged by size: 1753 1086 1073 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 2341 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2328 2336 2807 2822 4505 1239 Size: 823 266 8 471 15 1683 2579 Fragments arranged by size: 2579 1683 823 471 266 15 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2640 3636 493 Size: 711 1436 996 2702 Fragments arranged by size: 2702 1436 996 711 BsaBI GATnn'nnATC Cuts at: 2612 5839 2612 Size: 3227 2618 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2982 3652 4034 4669 Size: 53 83 186 936 1449 670 382 635 Cuts at: 4669 275 Size: 1451 Fragments arranged by size: 1451 1449 936 670 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2419 4278 4676 4950 5097 5508 2419 Size: 1859 398 274 147 411 2756 Fragments arranged by size: 2756 1859 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5620 Fragments arranged by size: 5620 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2420 2720 2819 2981 3122 1186 Size: 1147 87 300 99 162 141 3909 Fragments arranged by size: 3909 1147 300 162 141 99 87 BseYI C'CCAG_C Cuts at: 2111 2198 2218 2465 2561 2644 2729 5048 5544 Size: 87 20 247 96 83 85 2319 496 Cuts at: 5544 2111 Size: 2412 Fragments arranged by size: 2412 2319 496 247 96 87 85 83 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2931 3295 4056 5084 1540 Size: 275 186 930 364 761 1028 2301 Fragments arranged by size: 2301 1028 930 761 364 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 2457 2811 3906 3991 5062 2427 Size: 30 354 1095 85 1071 3210 Fragments arranged by size: 3210 1095 1071 354 85 30 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2327 2427 2457 2539 2639 2672 Size: 399 256 281 100 30 82 100 33 Cuts at: 2672 2811 3068 3142 3164 3906 3991 5062 1391 Size: 139 257 74 22 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 281 257 256 139 100 100 85 82 74 33 30 22 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2509 3200 844 Size: 1665 691 3489 Fragments arranged by size: 3489 1665 691 BsrBI CCG'CTC Cuts at: 1394 1823 3075 3732 1394 Size: 429 1252 657 3507 Fragments arranged by size: 3507 1252 657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4340 4514 65 Size: 1183 3092 174 1396 Fragments arranged by size: 3092 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4489 4676 1700 Size: 110 2679 187 2869 Fragments arranged by size: 2869 2679 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 2538 3905 4917 2538 Size: 1367 1012 3466 Fragments arranged by size: 3466 1367 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2782 794 Size: 1988 3857 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstYI r'GATC_y Cuts at: 1972 2899 3175 3542 3927 3944 5396 5482 5494 Size: 927 276 367 385 17 1452 86 12 Cuts at: 5494 5840 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 927 385 367 346 276 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2121 2712 513 Size: 552 1056 591 3646 Fragments arranged by size: 3646 1056 591 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3096 487 Size: 683 56 1870 3236 Fragments arranged by size: 3236 1870 683 56 BtsI GCAGTG_nn' Cuts at: 2244 2455 2968 3361 4155 4175 2244 Size: 211 513 393 794 20 3914 Fragments arranged by size: 3914 794 513 393 211 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DraIII CAC_nnn'GTG Cuts at: 3016 3016 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2177 4852 809 Size: 507 861 2675 1802 Fragments arranged by size: 2675 1802 861 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2530 2928 3132 Size: 54 1425 50 275 18 700 398 204 Cuts at: 3132 3292 4181 5810 8 Size: 160 889 1629 43 Fragments arranged by size: 1629 1425 889 700 398 275 204 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2928 3292 1537 Size: 275 1116 364 4090 Fragments arranged by size: 4090 1116 364 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 2292 2970 3775 1353 Size: 845 94 678 805 3423 Fragments arranged by size: 3423 845 805 678 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2455 2455 Size: 5845 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2062 2328 2336 2807 2822 2994 Size: 393 422 8 266 8 471 15 172 Cuts at: 2994 3908 4505 5292 5560 1239 Size: 914 597 787 268 1524 Fragments arranged by size: 1524 914 787 597 471 422 393 268 266 172 15 8 8 EcoO109I rG'GnC_Cy Cuts at: 2287 2519 2287 Size: 232 5613 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 2077 2387 2985 4992 1678 Size: 399 310 598 2007 2531 Fragments arranged by size: 2531 2007 598 399 310 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2592 2596 2624 2798 Size: 32 135 32 992 28 4 28 174 Cuts at: 2798 2830 4486 4518 4560 4592 1373 Size: 32 1656 32 42 32 2626 Fragments arranged by size: 2626 1656 992 174 135 42 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KasI G'GCGC_C Cuts at: 2981 2981 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3000 4959 5143 1305 Size: 1695 1959 184 2007 Fragments arranged by size: 2007 1959 1695 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4023 Fragments arranged by size: 4023 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2687 2880 3803 4162 4321 518 Size: 648 1521 193 923 359 159 2042 Fragments arranged by size: 2042 1521 923 648 359 193 159 MspA1I CmG'CkG Cuts at: 1606 3115 3261 3938 5086 5331 1606 Size: 1509 146 677 1148 245 2120 Fragments arranged by size: 2120 1509 1148 677 245 146 NarI GG'CG_CC Cuts at: 2982 2982 Size: 5845 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5293 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2723 3203 4748 1132 Size: 97 1494 480 1545 2229 Fragments arranged by size: 2229 1545 1494 480 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2477 2845 1108 Size: 1369 368 4108 Fragments arranged by size: 4108 1369 368 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PmlI CAC'GTG Cuts at: 2640 2640 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PstI C_TGCA'G Cuts at: 830 2462 2595 3197 830 Size: 1632 133 602 3478 Fragments arranged by size: 3478 1632 602 133 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 3115 3115 Size: 5845 SacI G_AGCT'C Cuts at: 2457 2457 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 2350 2458 2591 3193 4328 5009 5200 Size: 219 1305 108 133 602 1135 681 191 Cuts at: 5200 826 Size: 1471 Fragments arranged by size: 1471 1305 1135 681 602 219 191 133 108 SfoI GGC'GCC Cuts at: 2983 2983 Size: 5845 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 2723 3203 1132 Size: 1591 480 3774 Fragments arranged by size: 3774 1591 480 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 3123 1517 Size: 1606 4239 StyI C'CwwG_G Cuts at: 513 1065 3027 3284 513 Size: 552 1962 257 3074 Fragments arranged by size: 3074 1962 552 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3872 3889 4042 4227 4672 325 Size: 1255 2292 17 153 185 445 1498 Fragments arranged by size: 2292 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2511 4091 5803 Size: 274 80 33 51 1443 534 1580 1712 Cuts at: 5803 96 Size: 138 Fragments arranged by size: 1712 1580 1443 534 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5805 5837 5805 Size: 32 5813 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2119 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI