CWC25 ORF clone FHC10540

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10540

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Clone info.

Species

human

Clone Name

pFN21AB0960 Sequence (with vector) Restriction Map

Product ID

FHC10540

Flexi Clone PID

FXC10540

ORF Sequence

DNA sequence (1275 bp)
Protein sequence (425 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587360]

Gene

KIBB0960

Symbol

CWC25

Alias : CCDC49

Description

CWC25 spliceosome-associated protein homolog, transcript variant 1

Original Clone ID

cs02060

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1275 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 425 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45663.1 4.5e-91 100.0 CWC25 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019339 11 47 PF10197 CBF1-interacting co-repressor CIR IPR022209 64 157 PF12542 Pre-mRNA splicing factor Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB0960 Download

>KIBB0960  1275 bp
ATGGGGGGCGGAGACCTGAATCTGAAGAAGAGCTGGCACCCGCAGACCCTCAGGAATGTG
GAGAAAGTGTGGAAGGCCGAGCAGAAGCATGAGGCTGAGCGGAAGAAGATTGAGGAGCTT
CAGCGGGAGCTGCGAGAAGAGAGAGCCCGGGAAGAGATGCAGCGCTATGCGGAGGATGTT
GGGGCCGTCAAGAAAAAAGAAGAAAAGTTGGACTGGATGTACCAGGGTCCTGGTGGGATG
GTGAACCGTGACGAGTACCTGCTGGGGCGCCCCATTGACAAATATGTTTTTGAGAAGATG
GAGGAGAAGGAGGCAGGCTGCTCTTCTGAAACAGGACTTCTCCCAGGCTCTATCTTTGCC
CCATCAGGTGCCAATTCCCTTCTTGACATGGCCAGCAAGATCCGGGAGGACCCACTCTTC
ATCATCAGGAAGAAGGAGGAGGAGAAAAAACGAGAGGTATTAAATAATCCAGTGAAAATG
AAGAAAATCAAAGAATTGTTGCAAATGAGTCTGGAAAAAAAGGAGAAGAAGAAAAAGAAG
GAGAAGAAAAAGAAGCACAAGAAACATAAGCACAGAAGCTCGAGTAGTGATCGTTCCAGC
AGCGAGGATGAGCACAGTGCAGGGAGATCACAGAAGAAGATGGCAAATTCCTCCCCTGTT
TTGTCCAAAGTCCCTGGATATGGCTTACAGGTCAGGAACTCTGACCGTAACCAGGGTCTT
CAGGGTCCTCTGACAGCAGAGCAAAAGAGAGGGCATGGGATGAAGAACCATTCCAGATCC
AGAAGCTCCTCCCACTCACCCCCAAGACATGCCAGCAAGAAGAGCACCAGGGAAGCAGGG
TCCCGGGACAGGAGGTCTCGATCCCTGGGCAGAAGGTCACGGTCCCCAAGACCCAGCAAA
CTGCACAACTCTAAGGTGAACAGGAGAGAGACAGGCCAAACTAGGAGCCCATCACCTAAA
AAAGAGGTCTACCAAAGGCGACATGCTCCCGGATACACCAGAAAACTCTCTGCAGAGGAA
TTAGAGCGAAAACGGCAAGAGATGATGGAAAACGCCAAATGGAGGGAGGAGGAGAGACTG
AACATCCTCAAGAGGCATGCTAAGGATGAGGAACGGGAGCAGAGGCTAGAGAAGCTGGAC
TCCCGGGATGGGAAGTTCATCCACCGCATGAAGCTGGAGAGTGCATCTACTTCCTCCCTG
GAGGATCGGGTGAAGCGGAATATCTACTCTTTACAGAGAACTTCGGTAGCTCTGGAGAAG
AACTTTATGAAAAGA

ORF Protein

Cloned ORF protein sequence for pFN21AB0960 Download

>KIBB0960  425 aa
MGGGDLNLKKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDV
GAVKKKEEKLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKMEEKEAGCSSETGLLPGSIFA
PSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEKKKKK
EKKKKHKKHKHRSSSSDRSSSEDEHSAGRSQKKMANSSPVLSKVPGYGLQVRNSDRNQGL
QGPLTAEQKRGHGMKNHSRSRSSSHSPPRHASKKSTREAGSRDRRSRSLGRRSRSPRPSK
LHNSKVNRRETGQTRSPSPKKEVYQRRHAPGYTRKLSAEELERKRQEMMENAKWREEERL
NILKRHAKDEEREQRLEKLDSRDGKFIHRMKLESASTSSLEDRVKRNIYSLQRTSVALEK
NFMKR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB0960 Download

>pFN21AB0960  5980 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGGGCGGAGACCTGAATCTGAAGAAGAGCTG
GCACCCGCAGACCCTCAGGAATGTGGAGAAAGTGTGGAAGGCCGAGCAGAAGCATGAGGC
TGAGCGGAAGAAGATTGAGGAGCTTCAGCGGGAGCTGCGAGAAGAGAGAGCCCGGGAAGA
GATGCAGCGCTATGCGGAGGATGTTGGGGCCGTCAAGAAAAAAGAAGAAAAGTTGGACTG
GATGTACCAGGGTCCTGGTGGGATGGTGAACCGTGACGAGTACCTGCTGGGGCGCCCCAT
TGACAAATATGTTTTTGAGAAGATGGAGGAGAAGGAGGCAGGCTGCTCTTCTGAAACAGG
ACTTCTCCCAGGCTCTATCTTTGCCCCATCAGGTGCCAATTCCCTTCTTGACATGGCCAG
CAAGATCCGGGAGGACCCACTCTTCATCATCAGGAAGAAGGAGGAGGAGAAAAAACGAGA
GGTATTAAATAATCCAGTGAAAATGAAGAAAATCAAAGAATTGTTGCAAATGAGTCTGGA
AAAAAAGGAGAAGAAGAAAAAGAAGGAGAAGAAAAAGAAGCACAAGAAACATAAGCACAG
AAGCTCGAGTAGTGATCGTTCCAGCAGCGAGGATGAGCACAGTGCAGGGAGATCACAGAA
GAAGATGGCAAATTCCTCCCCTGTTTTGTCCAAAGTCCCTGGATATGGCTTACAGGTCAG
GAACTCTGACCGTAACCAGGGTCTTCAGGGTCCTCTGACAGCAGAGCAAAAGAGAGGGCA
TGGGATGAAGAACCATTCCAGATCCAGAAGCTCCTCCCACTCACCCCCAAGACATGCCAG
CAAGAAGAGCACCAGGGAAGCAGGGTCCCGGGACAGGAGGTCTCGATCCCTGGGCAGAAG
GTCACGGTCCCCAAGACCCAGCAAACTGCACAACTCTAAGGTGAACAGGAGAGAGACAGG
CCAAACTAGGAGCCCATCACCTAAAAAAGAGGTCTACCAAAGGCGACATGCTCCCGGATA
CACCAGAAAACTCTCTGCAGAGGAATTAGAGCGAAAACGGCAAGAGATGATGGAAAACGC
CAAATGGAGGGAGGAGGAGAGACTGAACATCCTCAAGAGGCATGCTAAGGATGAGGAACG
GGAGCAGAGGCTAGAGAAGCTGGACTCCCGGGATGGGAAGTTCATCCACCGCATGAAGCT
GGAGAGTGCATCTACTTCCTCCCTGGAGGATCGGGTGAAGCGGAATATCTACTCTTTACA
GAGAACTTCGGTAGCTCTGGAGAAGAACTTTATGAAAAGAGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT