Restriction map for
pFN21AB0960
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MaeII MamI MfeI MluI MroI
MslI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 967 GTCTAC GT^[AC][GT]AC
AcsI 645 AAATTC [GA]^AATT[CT]
AcyI 266 GGCGCC G[GA]^CG[CT]C
AhdI 847 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 48 CCTCAGG CC^T[ACGT]AGG
Aor51HI 161 AGCGCT AGC^GCT
ApoI 645 AAATTC [GA]^AATT[CT]
AspEI 847 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 389 TGGCCA TGG^CCA
BbeI 266 GGCGCC GGCGC^C
BbsI 716 GTCTTC GAAGAC(2/6)
BciVI 991 GGATAC GTATCC(6/5)
BlpI 94 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 94 GCTGAGC GC^T[ACGT]AGC
BpuAI 716 GTCTTC GAAGAC(2/6)
BsaHI 266 GGCGCC G[GA]^CG[CT]C
BspMI 257 ACCTGC ACCTGC(4/8)
BsrBI 97 GAGCGG CCGCTC(-3/-3)
BstXI 222 CCAGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 48 CCTCAGG CC^T[ACGT]AGG
CelII 94 GCTGAGC GC^T[ACGT]AGC
EaeI 389 TGGCCA [CT]^GGCC[GA]
Eam1105I 847 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 161 AGCGCT AGC^GCT
Eco81I 48 CCTCAGG CC^T[ACGT]AGG
Hin1I 266 GGCGCC G[GA]^CG[CT]C
KasI 266 GGCGCC G^GCGCC
MluNI 389 TGGCCA TGG^CCA
MscI 389 TGGCCA TGG^CCA
MseI 460 TTAA T^TAA
MspA1I 121 CAGCGG C[AC]G^C[GT]G
NarI 266 GGCGCC GG^CGCC
PaeR7I 579 CTCGAG C^TCGAG
PstI 1010 CTGCAG CTGCA^G
SfcI 1010 CTGCAG C^T[GA][CT]AG
SfoI 266 GGCGCC GGC^GCC
SphI 1095 GCATGC GCATG^C
TfiI 18 GAATC G^A[AT]TC
Tru9I 460 TTAA T^TAA
XcmI 222 CCAGGGTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 579 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 219 GTAC GT^AC
255 GTAC
AspHI 610 GAGCAC G[AT]GC[AT]^C
822 GAGCAC
BanII 143 GAGCCC G[GA]GC[CT]^C
945 GAGCCC
BfaI 941 CTAG C^TAG
1126 CTAG
BsaI 11 GAGACC GGTCTC(1/5)
854 GGTCTC
BsgI 901 CTGCAC GTGCAG(16/14)
617 GTGCAG
BsiHKAI 610 GAGCAC G[AT]GC[AT]^C
822 GAGCAC
BsrI 212 ACTGG ACTGG(1/-1)
469 CCAGT
BstYI 398 AGATCC [GA]^GATC[CT]
775 AGATCC
CfoI 162 GCGC GCG^C
267 GCGC
FauI 39 CCCGC CCCGC(4/6)
123 GCGGG
HaeII 161 AGCGCT [GA]GCGC^[CT]
266 GGCGCC
HhaI 162 GCGC GCG^C
267 GCGC
HinP1I 162 GCGC G^CGC
267 GCGC
MaeI 941 CTAG C^TAG
1126 CTAG
MflI 398 AGATCC [GA]^GATC[CT]
775 AGATCC
PleI 1138 GACTC GAGTC(4/5)
507 GAGTC
RsaI 219 GTAC GT^AC
255 GTAC
SfaNI 156 GATGC GCATC(5/9)
1183 GCATC
SmlI 579 CTCGAG C^T[CT][GA]AG
1087 CTCAAG
TaqI 580 TCGA T^CGA
858 TCGA
Tsp45I 248 GTGAC ^GT[GC]AC
876 GTCAC
TspRI 470 CAGTG CA[GC]TG[ACGT][ACGT]^
615 CAGTG
TthHB8I 580 TCGA T^CGA
858 TCGA
XhoII 398 AGATCC [GA]^GATC[CT]
775 AGATCC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanI BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PpuMI SapI Sau3AI Sau96I ScrFI
SmaI TseI Tsp509I XmaCI XmaI